##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515026
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739180157894943	31.0	31.0	34.0	30.0	34.0
2	31.9392516105983	33.0	31.0	34.0	30.0	34.0
3	32.095311693001904	34.0	31.0	34.0	30.0	34.0
4	35.77753161976288	37.0	35.0	37.0	35.0	37.0
5	35.5634705820677	37.0	35.0	37.0	33.0	37.0
6	35.58702084943285	37.0	35.0	37.0	33.0	37.0
7	35.813461844644735	37.0	35.0	37.0	35.0	37.0
8	35.874616038801925	37.0	35.0	37.0	35.0	37.0
9	37.57408558014547	39.0	37.0	39.0	35.0	39.0
10	37.049842143891766	39.0	37.0	39.0	33.0	39.0
11	37.005689033175024	39.0	37.0	39.0	33.0	39.0
12	37.05699323917627	39.0	37.0	39.0	33.0	39.0
13	37.07464283356568	39.0	37.0	39.0	33.0	39.0
14	38.25206688594362	40.0	38.0	41.0	33.0	41.0
15	38.218548189800124	40.0	38.0	41.0	33.0	41.0
16	38.12717416208114	40.0	37.0	41.0	33.0	41.0
17	38.121619102724914	40.0	37.0	41.0	33.0	41.0
18	38.067984917266315	40.0	37.0	41.0	33.0	41.0
19	38.14584700578223	40.0	37.0	41.0	34.0	41.0
20	38.06737135600921	40.0	37.0	41.0	33.0	41.0
21	38.06543553141007	40.0	37.0	41.0	33.0	41.0
22	38.052232314485096	40.0	37.0	41.0	33.0	41.0
23	37.916091614792265	40.0	37.0	41.0	33.0	41.0
24	37.796396686769214	40.0	37.0	41.0	33.0	41.0
25	37.88619215340585	40.0	37.0	41.0	33.0	41.0
26	37.81393754878395	40.0	37.0	41.0	33.0	41.0
27	37.775135624220916	40.0	37.0	41.0	33.0	41.0
28	37.77829662968472	40.0	37.0	41.0	33.0	41.0
29	37.62019975690548	40.0	37.0	41.0	33.0	41.0
30	37.614722363531165	40.0	37.0	41.0	33.0	41.0
31	37.588257680194786	40.0	37.0	41.0	33.0	41.0
32	37.476290517371936	39.0	36.0	41.0	32.0	41.0
33	37.46129127461526	39.0	36.0	41.0	32.0	41.0
34	37.43249467017199	39.0	36.0	41.0	32.0	41.0
35	37.31937416751776	39.0	36.0	41.0	32.0	41.0
36	37.23761713000897	39.0	36.0	41.0	32.0	41.0
37	37.12159386128079	39.0	36.0	41.0	31.0	41.0
38	37.0552593461301	39.0	36.0	41.0	31.0	41.0
39	36.94712111621549	39.0	35.0	41.0	31.0	41.0
40	36.890988027788886	39.0	35.0	41.0	31.0	41.0
41	36.80285849646425	39.0	35.0	40.0	31.0	41.0
42	36.685817803373034	39.0	35.0	40.0	30.0	41.0
43	36.569555711750475	39.0	35.0	40.0	30.0	41.0
44	36.4941575765107	39.0	35.0	40.0	30.0	41.0
45	36.283812856049984	39.0	35.0	40.0	30.0	41.0
46	36.141313254088146	38.0	35.0	40.0	30.0	41.0
47	36.01268285484616	38.0	35.0	40.0	29.0	41.0
48	35.909800670257425	38.0	35.0	40.0	29.0	41.0
49	35.74028107318854	38.0	34.0	40.0	28.0	41.0
50	35.64986039539751	38.0	34.0	40.0	28.0	41.0
51	34.30623696667741	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	9.0
13	5.0
14	11.0
15	19.0
16	40.0
17	62.0
18	111.0
19	238.0
20	366.0
21	586.0
22	902.0
23	1259.0
24	1723.0
25	2233.0
26	2713.0
27	3562.0
28	4800.0
29	6517.0
30	8449.0
31	11183.0
32	14805.0
33	19999.0
34	28837.0
35	37770.0
36	49291.0
37	73161.0
38	114971.0
39	131362.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.04423077669865	31.64112103078291	21.95578475649773	26.35886343602071
2	20.52517736968619	29.28415264472085	22.79515985600727	27.39551012958569
3	20.476441966036667	30.04877423664048	22.538279620834675	26.936504176488178
4	19.06952270370816	29.817329610543936	21.895593620516244	29.21755406523166
5	17.64202195617309	33.150559389234715	20.793513337190745	28.41390531740145
6	20.75798115046619	33.646068353830685	20.62847312562861	24.96747737007452
7	85.38695133837903	3.814953031497439	5.910187058517434	4.8879085716060935
8	88.47223246981706	2.7878204207166237	4.120568670319557	4.619378439146762
9	79.22726231297061	6.632869020204805	8.795478286533106	5.344390380291481
10	31.458994303200228	30.63379324538955	15.292820168302182	22.614392283108035
11	19.63221274265765	25.79947420130246	34.28661853964654	20.281694516393348
12	18.49401777774326	21.768221410181233	40.65600571621627	19.081755095859236
13	23.096309700869472	24.14751876604288	32.139736634655335	20.616434898432313
14	19.488919006030763	28.739325781611026	31.269100977426383	20.50265423493183
15	18.232283418701194	34.55204203282941	28.633700046211256	18.581974502258138
16	17.949190914633437	32.640293888075554	28.00577058245603	21.40474461483498
17	16.945940593290434	32.710387436750764	30.756505496809872	19.58716647314893
18	17.45271112526358	31.31686555630201	30.75553467203597	20.474888646398433
19	17.140299713024195	34.77707921541825	31.238617079526083	16.84400399203147
20	17.306699079269787	33.11696885205795	30.972416926524097	18.603915142148164
21	17.306504914315006	33.91479265124479	29.369973554733157	19.408728879707045
22	15.786969978214692	32.22885834889889	31.53840776970483	20.44576390318159
23	16.6321700263676	32.19216117244566	29.334829697918163	21.84083910326857
24	14.277337454808107	33.73188926384299	30.2776947183249	21.713078563024002
25	16.49703121784143	32.62495485664802	30.43069670269074	20.44731722281982
26	17.927056109788634	33.59713878522638	29.348032914843138	19.127772190141854
27	15.084092841914778	33.187256565687946	29.81771794045349	21.910932651943785
28	17.91793035691402	31.274343431205416	31.897224606136387	18.910501605744177
29	19.118258107357686	32.28691367037781	29.145907196918213	19.448921025346294
30	16.69876860585679	34.28525938496309	28.99659434669318	20.019377662486942
31	20.215872596723273	32.63466310438696	27.123679192895118	20.025785105994647
32	17.907251284401177	32.64553634185459	29.694617359123615	19.75259501462062
33	18.176363911724845	30.61961920369069	30.141196755115274	21.06282012946919
34	18.45168981760144	29.201438373985	32.404189303064314	19.942682505349243
35	17.807838827554338	31.168911860760428	30.822133251525162	20.20111606016007
36	20.312566744203206	31.100759961633006	30.755146342126416	17.831526952037375
37	19.18291503729909	33.51927863836001	27.740929584137504	19.556876740203407
38	19.842105058773733	31.720340332332736	30.23381343854485	18.203741170348682
39	18.68818273252224	29.896937242003318	30.444288249525265	20.970591775949178
40	19.54911014201225	31.762279962565	27.863836000512592	20.82477389491016
41	18.860601212365978	30.360797318970306	31.013191567027683	19.765409901636033
42	20.30208183664514	31.688691444703764	29.220854869462904	18.788371849188195
43	18.174616427131834	30.091684691646638	30.50913934442145	21.224559536800083
44	20.36537961190309	28.608652767044774	28.94397564394807	22.081991977104067
45	20.79623164655765	28.549626620791962	30.35943816428685	20.29470356836354
46	17.283593449651086	29.669958409866688	32.497582646313	20.548865494169227
47	18.927976451674283	29.886064004535694	29.552682777180184	21.63327676660984
48	19.20155487295787	29.29988000605795	32.48845689343839	19.010108227545793
49	19.565031668304126	27.93315288936869	30.750874713121277	21.750940729205904
50	18.519453386819308	27.560550341147827	28.87737706445888	25.042619207573985
51	18.485668684687763	26.969318053845825	32.5336973279019	22.011315933564518
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1137.0
1	882.5
2	628.0
3	459.0
4	290.0
5	324.5
6	359.0
7	397.0
8	435.0
9	498.5
10	562.0
11	679.0
12	796.0
13	920.0
14	1044.0
15	1248.0
16	1452.0
17	1723.5
18	1995.0
19	2281.0
20	2567.0
21	3354.0
22	4141.0
23	5028.5
24	5916.0
25	7355.0
26	10147.0
27	11500.0
28	14284.5
29	17069.0
30	19245.0
31	21421.0
32	22578.0
33	23735.0
34	25680.0
35	27625.0
36	28496.0
37	29367.0
38	29608.5
39	29850.0
40	29686.5
41	29523.0
42	30621.5
43	31720.0
44	34054.0
45	36388.0
46	41010.0
47	45632.0
48	48685.5
49	51739.0
50	48329.5
51	44920.0
52	37832.0
53	30744.0
54	25020.5
55	19297.0
56	15376.5
57	11456.0
58	9554.0
59	7652.0
60	6294.0
61	4936.0
62	4534.5
63	4133.0
64	3241.5
65	2350.0
66	1840.0
67	1330.0
68	1173.0
69	1016.0
70	719.5
71	423.0
72	381.5
73	340.0
74	333.0
75	258.5
76	191.0
77	146.0
78	101.0
79	73.0
80	45.0
81	48.0
82	51.0
83	30.0
84	9.0
85	9.5
86	10.0
87	7.0
88	4.0
89	4.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	515026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.606141720377266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.61606439314534	30.298704853648555
2	9.89138521652815	8.033019798263732
3	3.8708797729786393	4.715444779323373
4	2.1826358723421264	3.5451368622521455
5	1.4981472983435127	3.04169907572685
6	1.1132822711924177	2.712365860729368
7	0.866737757990136	2.4636413364744665
8	0.7443429604774189	2.4179916593368995
9	0.6067841853422763	2.2175248161322925
>10	4.4494363023949335	33.40941411366951
>50	0.12142785695845396	3.171249931076258
>100	0.03599640028389141	2.6353024562306517
>500	0.002399760018926094	0.6749040383025252
>1k	4.799520037852188E-4	0.6636004188334099
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	3405	0.6611316710224339	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	922	0.17902008830622143	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	918	0.1782434284871055	No Hit
AACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	579	0.1124215088170306	No Hit
TACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	539	0.10465491062587132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.824948643369461E-4	0.0	0.0	0.7822906028045186	0.0
2	5.824948643369461E-4	0.0	0.0	1.1912019975690549	0.0
3	5.824948643369461E-4	0.0	0.0	1.6661294769584447	0.0
4	5.824948643369461E-4	0.0	0.0	3.6287488398643952	0.0
5	5.824948643369461E-4	0.0	0.0	3.9357236333699657	0.0
6	5.824948643369461E-4	0.0	0.0	5.619910451122856	0.0
7	5.824948643369461E-4	0.0	0.0	6.804705005184204	0.0
8	5.824948643369461E-4	0.0	0.0	7.744657551269256	0.0
9	5.824948643369461E-4	0.0	0.0	9.179148237176376	0.0
10	5.824948643369461E-4	0.0	0.0	11.521554251630015	0.0
11	5.824948643369461E-4	0.0	0.0	13.751150427357066	0.0
12	5.824948643369461E-4	0.0	0.0	14.809737760812075	0.0
13	5.824948643369461E-4	0.0	0.0	15.206222598470756	0.0
14	5.824948643369461E-4	0.0	0.0	15.60251327117466	0.0
15	5.824948643369461E-4	0.0	0.0	15.986959881637043	0.0
16	5.824948643369461E-4	0.0	0.0	16.807306815578244	0.0
17	5.824948643369461E-4	0.0	0.0	18.025691906816355	0.0
18	5.824948643369461E-4	0.0	0.0	19.22427217266701	0.0
19	5.824948643369461E-4	0.0	0.0	19.893752936744942	0.0
20	5.824948643369461E-4	0.0	0.0	20.555467102631713	0.0
21	5.824948643369461E-4	0.0	0.0	21.238151083634612	0.0
22	5.824948643369461E-4	0.0	0.0	22.050537254429873	0.0
23	5.824948643369461E-4	0.0	0.0	22.84836105361671	0.0
24	5.824948643369461E-4	0.0	0.0	23.46658226963299	0.0
25	5.824948643369461E-4	0.0	0.0	24.04830047415082	0.0
26	5.824948643369461E-4	0.0	0.0	24.535654510646065	0.0
27	5.824948643369461E-4	0.0	0.0	25.09543207527387	0.0
28	5.824948643369461E-4	0.0	0.0	25.628997371006513	0.0
29	5.824948643369461E-4	0.0	0.0	26.15168942927153	0.0
30	5.824948643369461E-4	0.0	0.0	26.884079638697852	0.0
31	5.824948643369461E-4	0.0	0.0	27.415120790018367	0.0
32	5.824948643369461E-4	0.0	0.0	27.909270599930878	0.0
33	5.824948643369461E-4	0.0	0.0	28.394488821923552	0.0
34	5.824948643369461E-4	0.0	0.0	28.865338837262584	0.0
35	5.824948643369461E-4	0.0	0.0	29.34706209006924	0.0
36	5.824948643369461E-4	0.0	0.0	29.823348724142082	0.0
37	5.824948643369461E-4	0.0	0.0	30.3155568845068	0.0
38	5.824948643369461E-4	0.0	0.0	30.78834854939362	0.0
39	5.824948643369461E-4	0.0	0.0	31.287546648130384	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAG	20	7.029654E-4	45.000004	1
GCCGGCA	20	7.029654E-4	45.000004	23
CGCTCAG	60	0.0	45.000004	1
CACTTCG	20	7.029654E-4	45.000004	21
ACGCGCG	55	1.8189894E-12	44.999996	1
CTCACGG	155	0.0	42.096775	2
TATGGGT	120	0.0	41.250004	4
TACGCAG	60	3.6379788E-12	41.250004	1
CGCTCCG	45	1.9246727E-8	40.0	1
CTGTACG	40	3.4532604E-7	39.375004	1
ACGGGTC	160	0.0	39.375004	5
CGGATTA	40	3.4532604E-7	39.375004	40
TAGGGCC	195	0.0	39.23077	5
ACGTAGG	75	0.0	39.000004	2
AGGGCCC	145	0.0	38.793102	6
TAGCAGG	215	0.0	38.720932	2
GCCACGG	105	0.0	38.57143	2
CCTAGCG	35	6.2415766E-6	38.571426	1
CCATGCG	35	6.2415766E-6	38.571426	1
GTAGCAT	35	6.2415766E-6	38.571426	29
>>END_MODULE