FastQCFastQC Report
Sat 18 Jun 2016
SRR3553125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791275
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT48130.60825882278601No Hit
GCCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTC20310.2566743546807368No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT19690.24883889924488958No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC19140.24188809200341221No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT16770.21193643170831886No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC10610.1340873906037724No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8630.10906448453445389No Hit
GCCCCTGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAG8240.10413573030867904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGT207.0319534E-445.024
ACGTGTA253.8896993E-545.027
TCGTACG207.0319534E-445.012
CCGACAG406.8102963E-945.01
GTCCCGT207.0319534E-445.027
TAGTGCG207.0319534E-445.01
TATCCCG351.2112832E-745.01
CGGACCA207.0319534E-445.06
TAGCGCG650.041.5384641
CCCCTGG15250.041.4590152
ATCTACG603.6379788E-1241.2499961
GGCGAGT556.002665E-1140.909098
CGCTACG451.9270374E-840.01
AAGGGTC2100.039.6428575
ATAGGGC4850.039.432994
CACTGCG800.039.3751
TGCGCGA403.4564073E-739.37517
ACGTGCG403.4564073E-739.3751
ATAGGGA4200.039.1071434
GGGAATG30700.039.063527