##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	791275
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.770304887681274	31.0	31.0	34.0	30.0	34.0
2	31.988091371520646	33.0	31.0	34.0	30.0	34.0
3	32.1381542447316	34.0	31.0	34.0	30.0	34.0
4	35.80168967805125	37.0	35.0	37.0	35.0	37.0
5	35.5846665192253	37.0	35.0	37.0	33.0	37.0
6	35.5967255378977	37.0	35.0	37.0	33.0	37.0
7	35.750910871694416	37.0	35.0	37.0	35.0	37.0
8	35.81031499794635	37.0	35.0	37.0	35.0	37.0
9	37.4164152791381	39.0	37.0	39.0	35.0	39.0
10	37.01537518561815	39.0	37.0	39.0	33.0	39.0
11	37.01429591482102	39.0	37.0	39.0	33.0	39.0
12	36.99825345170769	39.0	37.0	39.0	33.0	39.0
13	36.9887940349436	39.0	37.0	39.0	33.0	39.0
14	38.14688824997631	40.0	37.0	41.0	33.0	41.0
15	38.172904489589584	40.0	37.0	41.0	33.0	41.0
16	38.06650153233705	40.0	37.0	41.0	33.0	41.0
17	38.02816340715933	40.0	37.0	41.0	33.0	41.0
18	37.96879719440144	40.0	37.0	41.0	33.0	41.0
19	38.06100407570061	40.0	37.0	41.0	33.0	41.0
20	37.97605130959527	40.0	37.0	41.0	33.0	41.0
21	37.95010963318695	40.0	37.0	41.0	33.0	41.0
22	37.921970237907175	40.0	37.0	41.0	33.0	41.0
23	37.78497235474393	40.0	37.0	41.0	33.0	41.0
24	37.72196518277464	40.0	37.0	41.0	33.0	41.0
25	37.68354617547629	40.0	37.0	41.0	33.0	41.0
26	37.672304824492116	40.0	37.0	41.0	33.0	41.0
27	37.66086758712205	40.0	37.0	41.0	33.0	41.0
28	37.49816814634609	40.0	36.0	41.0	32.0	41.0
29	37.33785725569492	40.0	36.0	41.0	32.0	41.0
30	37.40812233420745	40.0	36.0	41.0	32.0	41.0
31	37.325488610154494	39.0	36.0	41.0	32.0	41.0
32	37.33001421756027	40.0	36.0	41.0	32.0	41.0
33	37.23112950617674	39.0	36.0	41.0	31.0	41.0
34	37.225951786673406	39.0	36.0	41.0	31.0	41.0
35	37.14755552747149	39.0	36.0	41.0	31.0	41.0
36	37.03623013490885	39.0	35.0	41.0	31.0	41.0
37	36.9930011689994	39.0	35.0	41.0	31.0	41.0
38	36.90914915800449	39.0	35.0	41.0	31.0	41.0
39	36.82445673122492	39.0	35.0	41.0	31.0	41.0
40	36.729809484692424	39.0	35.0	41.0	31.0	41.0
41	36.6820763956905	39.0	35.0	40.0	31.0	41.0
42	36.62454266847809	39.0	35.0	40.0	30.0	41.0
43	36.56087201036302	39.0	35.0	40.0	30.0	41.0
44	36.51918233231178	39.0	35.0	40.0	30.0	41.0
45	36.36721367413352	39.0	35.0	40.0	30.0	41.0
46	36.240140279927964	38.0	35.0	40.0	30.0	41.0
47	36.103396417174814	38.0	35.0	40.0	30.0	41.0
48	36.02919086284793	38.0	35.0	40.0	30.0	41.0
49	35.86721304224195	38.0	35.0	40.0	29.0	41.0
50	35.7605080408202	38.0	34.0	40.0	29.0	41.0
51	34.556926479416134	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	6.0
12	6.0
13	8.0
14	13.0
15	24.0
16	54.0
17	92.0
18	160.0
19	381.0
20	833.0
21	1380.0
22	1940.0
23	2390.0
24	3008.0
25	3687.0
26	4511.0
27	5829.0
28	7539.0
29	9803.0
30	13237.0
31	17597.0
32	23310.0
33	31378.0
34	46930.0
35	59335.0
36	72752.0
37	108319.0
38	170379.0
39	206310.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.701273261508323	31.20849262266595	22.04239992417301	26.047834191652715
2	22.10053394837446	29.10227165018483	21.461565195412465	27.335629206028244
3	20.458626899624026	28.701652396448768	22.020157341000285	28.81956336292692
4	18.961296641496318	28.97033269091024	21.008751698208588	31.059618969384857
5	17.437300559224038	33.56683833054248	19.182079555148338	29.813781555085146
6	19.91204069381694	34.30728886923004	22.80484028940634	22.97583014754668
7	85.67173233073204	4.283845692079239	5.524501595526208	4.519920381662507
8	87.76430444535718	3.034975198255979	3.9898897349214875	5.210830621465357
9	79.4406495845313	6.626899624024516	8.421977188714417	5.510473602729771
10	33.306246248143815	28.83283308584247	14.997819974092447	22.863100691921268
11	22.685791918106855	28.321885564437142	28.755363179678366	20.236959337777638
12	21.065116426021294	22.19127357745411	37.770307415247544	18.973302581277053
13	25.753372721241035	24.237591229345046	29.21247353954061	20.796562509873308
14	19.636409592113992	29.83223278885343	29.422893431487157	21.108464187545415
15	19.152127894853244	32.242519983570816	28.855454803955645	19.749897317620295
16	19.243120280559857	31.005529051214815	27.771508009225617	21.979842658999715
17	17.531452402767687	30.229945341379423	29.783703516476574	22.454898739376322
18	19.987235790338378	29.376386212126	29.24293071308963	21.393447284445987
19	19.698840478973807	32.98840478973808	29.543331964234937	17.76942276705317
20	19.46087011468832	30.967362800543423	28.71694417238002	20.854822912388236
21	19.86313228649964	31.880572493760074	28.875169820858744	19.38112539888155
22	17.889861299800955	31.104736027297715	29.116173264667783	21.889229408233547
23	18.403083630848947	30.39954503807147	27.698840478973807	23.498530852105777
24	16.568070519098924	31.41676408328331	28.60522574326246	23.409939654355313
25	17.557612713658337	31.88878708413636	28.688761808473668	21.864838393731638
26	19.672427411456194	30.630564595115477	28.85090518467031	20.846102808758015
27	17.383716154307923	30.084989415816242	28.16555559066064	24.36573883921519
28	19.006919212663107	29.863953745537263	30.649774098764652	20.479352943034975
29	19.627815866797256	32.1345929038577	26.709045527787435	21.528545701557615
30	19.05393194527819	30.3021073583773	28.397586174212503	22.246374522132
31	19.063031183848853	33.253040978168144	26.09320400619254	21.590723831790466
32	19.981675144545196	29.162301349088498	27.274209345676283	23.581814160690026
33	18.356070898233863	31.079207607974475	28.74196707844934	21.822754415342327
34	16.866828852168968	29.66579254999842	30.033047928975385	23.43433066885722
35	19.808789611702633	29.471422703864015	29.32305456383685	21.396733120596505
36	19.079586742914916	31.01526018135288	28.70329531452403	21.201857761208174
37	18.668604467473383	32.13977441471044	25.796973239392116	23.394647878424063
38	19.153644434615018	32.71353195791602	27.460617358061356	20.6722062494076
39	18.73229913746801	31.269912483017915	27.321601213231812	22.676187166282265
40	19.79387697071183	32.900950996808945	26.437079397175445	20.868092635303782
41	18.879277116046886	30.039240466335976	29.472180973744905	21.60930144387223
42	20.87251587627563	29.323939212031213	28.439038261034405	21.364506650658747
43	19.06442134529715	27.66244352469116	30.312091245142337	22.961043884869355
44	19.928343496256044	26.977220308994976	28.578433540804397	24.516002653944582
45	20.433603993554705	28.558339388960853	29.50402830874222	21.50402830874222
46	17.481659347256013	29.032005307889165	31.47439259423083	22.01194275062399
47	18.977599443935418	30.693374616915737	28.430065400777227	21.898960538371615
48	19.06935009952292	28.7728033869388	30.521879245521465	21.63596726801681
49	20.476067106884457	28.00783545543585	28.919528608890715	22.59656882878898
50	18.550187987741303	27.756216233294367	28.08530536159995	25.608290417364383
51	18.856023506366306	26.15007424725917	31.441534232725665	23.55236801364886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1138.0
1	896.0
2	654.0
3	477.0
4	300.0
5	310.0
6	320.0
7	369.5
8	419.0
9	474.0
10	529.0
11	659.5
12	790.0
13	867.0
14	944.0
15	1193.5
16	1443.0
17	1893.0
18	2343.0
19	2915.5
20	3488.0
21	4122.5
22	4757.0
23	6874.5
24	8992.0
25	11171.0
26	15057.0
27	16764.0
28	18391.0
29	20018.0
30	23616.0
31	27214.0
32	29706.0
33	32198.0
34	34562.0
35	36926.0
36	39442.5
37	41959.0
38	41878.0
39	41797.0
40	41686.5
41	41576.0
42	43037.0
43	44498.0
44	49392.0
45	54286.0
46	58632.0
47	62978.0
48	66787.5
49	70597.0
50	67704.5
51	64812.0
52	55697.0
53	46582.0
54	41141.5
55	35701.0
56	30399.5
57	25098.0
58	23193.0
59	21288.0
60	19547.5
61	17807.0
62	16321.0
63	14835.0
64	12384.0
65	9933.0
66	8656.5
67	7380.0
68	6695.0
69	6010.0
70	5317.5
71	4625.0
72	3815.5
73	3006.0
74	2485.5
75	1463.0
76	961.0
77	685.5
78	410.0
79	327.0
80	244.0
81	206.0
82	168.0
83	123.5
84	79.0
85	50.5
86	22.0
87	38.0
88	54.0
89	31.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	791275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.267743291015048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9297594426486	22.67954723667599
2	9.686964492609224	5.864051090629476
3	3.665866533492996	3.3287252152466764
4	1.9661894086656042	2.3804846513201277
5	1.2809804237560503	1.9386193313531905
6	0.9323338800389953	1.6931785525521796
7	0.755150691452878	1.5999695092449757
8	0.5866381362260967	1.4204970009608808
9	0.5072829637562958	1.381887947059277
>10	4.720390144732798	31.01819879447633
>50	0.7855202661299233	16.506958309058323
>100	0.17703641963800054	7.793106970318253
>500	0.0033641124871829084	0.6809486230352559
>1k	0.002523084365387181	1.7138267680691068
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	4813	0.60825882278601	No Hit
GCCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTC	2031	0.2566743546807368	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	1969	0.24883889924488958	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	1914	0.24188809200341221	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGT	1677	0.21193643170831886	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1061	0.1340873906037724	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	863	0.10906448453445389	No Hit
GCCCCTGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAG	824	0.10413573030867904	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.527566269628132E-4	0.0	0.0	0.6871188903984077	0.0
2	2.527566269628132E-4	0.0	0.0	1.0018008909671101	0.0
3	2.527566269628132E-4	0.0	0.0	1.38005118321696	0.0
4	2.527566269628132E-4	0.0	0.0	2.9974408391520013	0.0
5	2.527566269628132E-4	0.0	0.0	3.2383179046475625	0.0
6	2.527566269628132E-4	0.0	0.0	4.51840384190073	0.0
7	2.527566269628132E-4	0.0	0.0	5.427948564026413	0.0
8	2.527566269628132E-4	0.0	0.0	6.2961675776436765	0.0
9	2.527566269628132E-4	0.0	0.0	7.5662696281318125	0.0
10	2.527566269628132E-4	0.0	0.0	9.423904457995008	0.0
11	2.527566269628132E-4	0.0	0.0	11.207987109412025	0.0
12	3.791349404442198E-4	0.0	0.0	12.019967773530063	0.0
13	3.791349404442198E-4	0.0	0.0	12.392404663359768	0.0
14	3.791349404442198E-4	0.0	0.0	12.710751635019431	0.0
15	3.791349404442198E-4	0.0	0.0	12.97930555116742	0.0
16	3.791349404442198E-4	0.0	0.0	13.630533000537108	0.0
17	3.791349404442198E-4	0.0	0.0	14.512400872010364	0.0
18	3.791349404442198E-4	0.0	0.0	15.772392657419987	0.0
19	3.791349404442198E-4	0.0	0.0	16.216486050993648	0.0
20	3.791349404442198E-4	0.0	0.0	16.821459037629143	0.0
21	3.791349404442198E-4	0.0	0.0	17.337967204827653	0.0
22	3.791349404442198E-4	0.0	0.0	17.9153897191242	0.0
23	3.791349404442198E-4	0.0	0.0	18.51050519730814	0.0
24	3.791349404442198E-4	0.0	0.0	18.95535686076269	0.0
25	3.791349404442198E-4	0.0	0.0	19.34156898676187	0.0
26	3.791349404442198E-4	0.0	0.0	19.676850652428044	0.0
27	3.791349404442198E-4	0.0	0.0	20.201447031689362	0.0
28	3.791349404442198E-4	0.0	0.0	20.635809295124957	0.0
29	3.791349404442198E-4	0.0	0.0	21.02783482354428	0.0
30	3.791349404442198E-4	0.0	0.0	21.66768822470064	0.0
31	3.791349404442198E-4	0.0	0.0	22.049603488041452	0.0
32	3.791349404442198E-4	0.0	0.0	22.40535844049161	0.0
33	3.791349404442198E-4	0.0	0.0	22.776910682126946	0.0
34	3.791349404442198E-4	0.0	0.0	23.113582509241414	0.0
35	3.791349404442198E-4	0.0	0.0	23.467568165302833	0.0
36	3.791349404442198E-4	0.0	0.0	23.800069508072415	0.0
37	3.791349404442198E-4	0.0	0.0	24.13105431108022	0.0
38	3.791349404442198E-4	0.0	0.0	24.50829357682222	0.0
39	3.791349404442198E-4	0.0	0.0	24.873147767843037	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGGT	20	7.0319534E-4	45.0	24
ACGTGTA	25	3.8896993E-5	45.0	27
TCGTACG	20	7.0319534E-4	45.0	12
CCGACAG	40	6.8102963E-9	45.0	1
GTCCCGT	20	7.0319534E-4	45.0	27
TAGTGCG	20	7.0319534E-4	45.0	1
TATCCCG	35	1.2112832E-7	45.0	1
CGGACCA	20	7.0319534E-4	45.0	6
TAGCGCG	65	0.0	41.538464	1
CCCCTGG	1525	0.0	41.459015	2
ATCTACG	60	3.6379788E-12	41.249996	1
GGCGAGT	55	6.002665E-11	40.90909	8
CGCTACG	45	1.9270374E-8	40.0	1
AAGGGTC	210	0.0	39.642857	5
ATAGGGC	485	0.0	39.43299	4
CACTGCG	80	0.0	39.375	1
TGCGCGA	40	3.4564073E-7	39.375	17
ACGTGCG	40	3.4564073E-7	39.375	1
ATAGGGA	420	0.0	39.107143	4
GGGAATG	3070	0.0	39.06352	7
>>END_MODULE