SRR3553124_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	922637
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	5320	0.5766081351604152	Illumina Single End Adapter 2 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	1929	0.20907464148955654	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	1668	0.18078615967059636	No Hit
AACCGAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATC	1512	0.1638781015719075	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGT	1435	0.15553245751037517	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC	1395	0.15119705799789082	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	1387	0.15032997809539395	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1253	0.13580638972857148	No Hit
AACCGAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGA	1043	0.11304554228802877	No Hit
GACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	925	0.10025611372620001	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCACGC	30	2.165034E-6	45.000004	8
AGCGCGC	20	7.032562E-4	45.0	30
AACCGAG	1435	0.0	41.236935	1
ACCGAGG	1520	0.0	40.11513	2
CGCTACG	45	1.927583E-8	40.000004	1
CGAGGGA	1695	0.0	39.955753	4
AAGCGCG	80	0.0	39.375	1
ACTAGCG	40	3.4572258E-7	39.375	1
ACTAGGG	880	0.0	39.11932	3
CGCCTCG	35	6.2469517E-6	38.571426	1
TACTACG	35	6.2469517E-6	38.571426	1
AGGGAAT	2605	0.0	37.744724	6
CACTAGG	310	0.0	37.74194	2
GGGAATG	3340	0.0	37.58982	7
AATGCGC	30	1.1397471E-4	37.500004	37
CGCTATG	60	1.546141E-10	37.500004	1
CGACTAG	60	1.546141E-10	37.500004	1
CATACGG	230	0.0	37.173916	2
CAACGGG	840	0.0	36.964287	3
CCGAGGG	1775	0.0	36.887325	3