##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	922637
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.701992224460973	31.0	31.0	34.0	30.0	34.0
2	31.91165648028423	33.0	31.0	34.0	30.0	34.0
3	32.08269557800088	34.0	31.0	34.0	30.0	34.0
4	35.75073512117984	37.0	35.0	37.0	35.0	37.0
5	35.52047013072313	37.0	35.0	37.0	33.0	37.0
6	34.866642027146106	37.0	35.0	37.0	32.0	37.0
7	35.38368285685486	37.0	35.0	37.0	33.0	37.0
8	35.80076238000427	37.0	35.0	37.0	35.0	37.0
9	37.415911132980796	39.0	37.0	39.0	35.0	39.0
10	36.99078185678658	39.0	37.0	39.0	33.0	39.0
11	36.97716545076774	39.0	37.0	39.0	33.0	39.0
12	36.93857931125676	39.0	37.0	39.0	33.0	39.0
13	36.91117850248798	39.0	37.0	39.0	33.0	39.0
14	38.08262187620917	40.0	37.0	41.0	33.0	41.0
15	38.06996901273198	40.0	37.0	41.0	33.0	41.0
16	37.96301687445875	40.0	37.0	41.0	33.0	41.0
17	37.981751219602074	40.0	37.0	41.0	33.0	41.0
18	37.95560225744253	40.0	37.0	41.0	33.0	41.0
19	37.99481377833319	40.0	37.0	41.0	33.0	41.0
20	37.90720510883479	40.0	37.0	41.0	33.0	41.0
21	37.882821738126694	40.0	37.0	41.0	33.0	41.0
22	37.866116359955214	40.0	37.0	41.0	33.0	41.0
23	37.77614056232299	40.0	37.0	41.0	33.0	41.0
24	37.66676601957216	39.0	36.0	41.0	32.0	41.0
25	37.66411275507052	39.0	37.0	41.0	33.0	41.0
26	37.654392789363534	39.0	37.0	41.0	33.0	41.0
27	37.61421013898207	40.0	37.0	41.0	33.0	41.0
28	37.46650199374185	39.0	36.0	41.0	32.0	41.0
29	37.29253650135427	39.0	36.0	41.0	32.0	41.0
30	37.348964977558886	39.0	36.0	41.0	32.0	41.0
31	37.28898472530367	39.0	36.0	41.0	32.0	41.0
32	37.30449244935983	39.0	36.0	41.0	32.0	41.0
33	37.08244087327952	39.0	36.0	41.0	31.0	41.0
34	37.1363201345708	39.0	36.0	41.0	31.0	41.0
35	37.053944292283965	39.0	36.0	41.0	31.0	41.0
36	37.06235713503794	39.0	36.0	41.0	31.0	41.0
37	36.93972385672805	39.0	35.0	41.0	31.0	41.0
38	36.78446669708672	39.0	35.0	41.0	31.0	41.0
39	36.766021739860854	39.0	35.0	40.0	31.0	41.0
40	36.723416685001794	39.0	35.0	40.0	31.0	41.0
41	36.672176598163745	39.0	35.0	40.0	31.0	41.0
42	36.5910146677404	39.0	35.0	40.0	30.0	41.0
43	36.56821263400449	39.0	35.0	40.0	30.0	41.0
44	36.49068160067285	39.0	35.0	40.0	30.0	41.0
45	36.28985288905604	38.0	35.0	40.0	30.0	41.0
46	36.151891805769765	38.0	35.0	40.0	30.0	41.0
47	36.03814826416023	38.0	35.0	40.0	30.0	41.0
48	35.94391943960626	38.0	35.0	40.0	29.0	41.0
49	35.84337610566236	38.0	35.0	40.0	29.0	41.0
50	35.74644307566248	38.0	34.0	40.0	29.0	41.0
51	34.54881172118612	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	7.0
12	9.0
13	8.0
14	15.0
15	29.0
16	62.0
17	103.0
18	237.0
19	477.0
20	965.0
21	1645.0
22	2388.0
23	2993.0
24	3738.0
25	4339.0
26	5361.0
27	6880.0
28	8866.0
29	11795.0
30	15608.0
31	20737.0
32	27929.0
33	37643.0
34	56376.0
35	70682.0
36	87721.0
37	130275.0
38	197828.0
39	227840.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.046778960739704	32.87078233368052	20.892507020637584	26.18993168494218
2	20.546000214602277	30.90803858939106	22.033367402347835	26.51259379365883
3	20.40824289509309	28.838969172057915	22.408487845165542	28.344300087683454
4	19.24137011630793	28.627618445824304	21.3867425650608	30.74426887280697
5	19.171678569144746	31.885020869529402	20.594556689142102	28.34874387218375
6	19.336857290570396	36.07702704313831	20.636935219376635	23.94918044691466
7	84.05364189816797	4.419181108063085	6.577126215402157	4.95005077836679
8	87.10933985955474	2.806846029370164	4.478359311408496	5.6054547996666075
9	79.11670570332645	6.000409695253929	9.31915802206068	5.5637265793589465
10	34.06767775408964	26.793636067055626	15.612315569395113	23.526370609459622
11	22.866306033683887	26.57090491710174	30.05233911061447	20.510449938599905
12	21.3348261558988	20.473382272768166	38.567388908097115	19.62440266323592
13	25.521629850092726	23.486701703920392	29.87653866038323	21.115129785603656
14	20.27926475959668	28.21543033717486	30.054723580346334	21.45058132288213
15	18.985039620132294	32.15760911387686	29.513448951212666	19.343902314778187
16	18.89258722552857	31.06649744157236	27.991615337342857	22.049299995556215
17	17.671955492788605	30.235726509992556	30.22109453663792	21.87122346058092
18	19.22402851825799	29.60264979618203	30.009527040428686	21.163794645131293
19	18.679285569514338	33.81795874217054	30.004649715977138	17.49810597233798
20	18.876763017307997	30.968300642614594	30.18164240107431	19.9732939390031
21	19.181324833060025	32.29785928810573	28.56681446766171	19.95400141117254
22	16.986745599840457	31.38168098612997	30.084204297031224	21.547369116998343
23	18.908411433749134	29.944712817717047	28.057079869981365	23.08979587855245
24	16.13245512590542	31.508274651894514	28.974016866871803	23.38525335532826
25	17.70208651940037	30.89416531095111	29.615439224743863	21.788308944904657
26	18.598863908557753	30.546791425013303	28.95082247948001	21.90352218694893
27	15.882736113986324	29.387613980362808	29.787988125340735	24.94166178031013
28	18.039055446508215	30.219685531796365	31.229725233217398	20.511533788478026
29	19.00270637314567	31.725803322433414	26.591172909822607	22.68031739459831
30	18.65619956711036	30.518069403243096	30.32275965520568	20.502971374440868
31	19.26456450369972	32.27271397093332	26.379497028625558	22.083224496741405
32	20.708902851283874	29.40528073337618	28.27623431533745	21.60958210000249
33	19.36482061742592	29.788204895316362	29.70041305518855	21.146561432069166
34	19.247006135674162	27.80866147791602	30.87552309304743	22.068809293362396
35	19.06340196632045	29.238367852145537	30.24623985381033	21.45199032772369
36	19.700272154704397	29.758507408655845	31.081996494829493	19.459223941810265
37	19.629605142650902	32.2493028135659	27.8792201049817	20.241871938801502
38	22.44859029065602	31.71724090839626	27.870224150993295	17.963944649954424
39	22.77645487878765	29.04847735349872	27.86751452629799	20.307553241415636
40	23.293559655639218	30.334031693938137	26.38058087850368	19.991827771918967
41	19.50203601199605	28.917223133258258	30.357659621281176	21.223081233464516
42	19.81982079626115	29.80175301879287	29.49892536284584	20.879500822100134
43	17.32891700636328	28.478372317607032	30.77938560885809	23.4133250671716
44	20.354483941138284	26.845118936266378	28.985722445555513	23.814674677039832
45	21.39259535440265	28.224534676151077	29.63234728284255	20.750522686603723
46	19.025142065622774	27.721411562727273	32.0586536200044	21.194792751645554
47	20.084388551510507	28.912996118733584	29.194797087045067	21.807818242710837
48	20.48617170132999	27.159218630945865	31.794736174681915	20.559873493042225
49	19.731595416182095	27.03663520973037	30.146092125071938	23.085677249015593
50	19.07900940456539	25.961456130634257	29.13746142849246	25.822073036307884
51	19.764869607440414	25.255219550050562	32.05583561032129	22.92407523218774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1539.0
1	1236.5
2	934.0
3	659.5
4	385.0
5	411.5
6	438.0
7	515.0
8	592.0
9	707.5
10	823.0
11	996.0
12	1169.0
13	1356.5
14	1544.0
15	1804.5
16	2065.0
17	2433.0
18	2801.0
19	3621.0
20	4441.0
21	5609.0
22	6777.0
23	7946.5
24	9116.0
25	11353.5
26	16595.5
27	19600.0
28	23037.5
29	26475.0
30	28772.0
31	31069.0
32	34149.5
33	37230.0
34	39834.0
35	42438.0
36	43923.0
37	45408.0
38	46265.5
39	47123.0
40	48224.0
41	49325.0
42	49656.0
43	49987.0
44	55487.0
45	60987.0
46	70058.5
47	79130.0
48	84887.5
49	90645.0
50	83272.5
51	75900.0
52	67059.5
53	58219.0
54	48669.5
55	39120.0
56	34071.0
57	29022.0
58	25632.0
59	22242.0
60	21285.5
61	20329.0
62	18322.0
63	16315.0
64	14046.0
65	11777.0
66	10054.0
67	8331.0
68	6746.5
69	5162.0
70	4351.5
71	3541.0
72	2966.5
73	2392.0
74	2220.0
75	1616.5
76	1185.0
77	857.0
78	529.0
79	557.5
80	586.0
81	373.5
82	161.0
83	136.0
84	111.0
85	61.5
86	12.0
87	10.0
88	8.0
89	7.0
90	6.0
91	6.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	922637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.936754845576345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16291645221085	24.426857978194292
2	10.15899908057848	6.6920892438689785
3	3.901980688951846	3.8555574409254008
4	2.025101080686419	2.6680103132832125
5	1.3534837757670126	2.228968165495156
6	0.9765786242735001	1.929919844107589
7	0.7331005436333164	1.6902167017966503
8	0.6236079126265701	1.6431696750354348
9	0.5274706173143642	1.5635853369655228
>10	4.880213618561264	33.415543061595784
>50	0.5650532423044163	12.673044331142647
>100	0.08445633516179736	4.449423542775754
>500	0.004021730245798444	0.8934612026202897
>1k	0.002681153497198963	1.282901667513344
>5k	3.3514418714987036E-4	0.5872514946800026
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	5320	0.5766081351604152	Illumina Single End Adapter 2 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	1929	0.20907464148955654	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	1668	0.18078615967059636	No Hit
AACCGAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATC	1512	0.1638781015719075	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGT	1435	0.15553245751037517	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCC	1395	0.15119705799789082	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	1387	0.15032997809539395	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1253	0.13580638972857148	No Hit
AACCGAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGA	1043	0.11304554228802877	No Hit
GACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	925	0.10025611372620001	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1676997562421625E-4	0.0	0.0	0.6388211181645652	0.0
2	2.1676997562421625E-4	0.0	0.0	1.0065713818110482	0.0
3	2.1676997562421625E-4	0.0	0.0	1.4479150521819524	0.0
4	2.1676997562421625E-4	0.0	0.0	3.4569391862671885	0.0
5	2.1676997562421625E-4	0.0	0.0	3.759224917275158	0.0
6	2.1676997562421625E-4	0.0	0.0	5.420658395446964	0.0
7	2.1676997562421625E-4	0.0	0.0	6.630451629405714	0.0
8	2.1676997562421625E-4	0.0	0.0	7.637781706131447	0.0
9	2.1676997562421625E-4	0.0	0.0	9.086888993179333	0.0
10	2.1676997562421625E-4	0.0	0.0	11.445888252909866	0.0
11	2.1676997562421625E-4	0.0	0.0	13.545847391769462	0.0
12	2.1676997562421625E-4	0.0	0.0	14.544615054458037	0.0
13	2.1676997562421625E-4	0.0	0.0	14.964173342278707	0.0
14	2.1676997562421625E-4	0.0	0.0	15.338426705193918	0.0
15	2.1676997562421625E-4	0.0	0.0	15.660655273959314	0.0
16	2.1676997562421625E-4	0.0	0.0	16.347165786761206	0.0
17	2.1676997562421625E-4	0.0	0.0	17.3487514591329	0.0
18	2.1676997562421625E-4	0.0	0.0	18.602548998143366	0.0
19	2.1676997562421625E-4	0.0	0.0	19.13439413333738	0.0
20	2.1676997562421625E-4	0.0	0.0	19.832718609810794	0.0
21	2.1676997562421625E-4	0.0	0.0	20.414637609374	0.0
22	2.1676997562421625E-4	0.0	0.0	21.081747209357527	0.0
23	2.1676997562421625E-4	0.0	0.0	21.772267966708466	0.0
24	2.1676997562421625E-4	0.0	0.0	22.291973983267525	0.0
25	2.1676997562421625E-4	0.0	0.0	22.75022571173712	0.0
26	2.1676997562421625E-4	0.0	0.0	23.159270655740016	0.0
27	2.1676997562421625E-4	0.0	0.0	23.705856149276475	0.0
28	3.2515496343632434E-4	0.0	0.0	24.15587061867235	0.0
29	3.2515496343632434E-4	0.0	0.0	24.59277050454296	0.0
30	4.335399512484325E-4	0.0	0.0	25.304317949529448	0.0
31	4.335399512484325E-4	0.0	0.0	25.744144230070983	0.0
32	4.335399512484325E-4	0.0	0.0	26.16662891256258	0.0
33	4.335399512484325E-4	0.0	0.0	26.598868243957266	0.0
34	4.335399512484325E-4	0.0	0.0	27.004011328398928	0.0
35	4.335399512484325E-4	0.0	0.0	27.425520546000214	0.0
36	4.335399512484325E-4	0.0	0.0	27.837383499686226	0.0
37	4.335399512484325E-4	0.0	0.0	28.250330303250358	0.0
38	4.335399512484325E-4	0.0	0.0	28.66175971698512	0.0
39	4.335399512484325E-4	0.0	0.0	29.079475460012983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCACGC	30	2.165034E-6	45.000004	8
AGCGCGC	20	7.032562E-4	45.0	30
AACCGAG	1435	0.0	41.236935	1
ACCGAGG	1520	0.0	40.11513	2
CGCTACG	45	1.927583E-8	40.000004	1
CGAGGGA	1695	0.0	39.955753	4
AAGCGCG	80	0.0	39.375	1
ACTAGCG	40	3.4572258E-7	39.375	1
ACTAGGG	880	0.0	39.11932	3
CGCCTCG	35	6.2469517E-6	38.571426	1
TACTACG	35	6.2469517E-6	38.571426	1
AGGGAAT	2605	0.0	37.744724	6
CACTAGG	310	0.0	37.74194	2
GGGAATG	3340	0.0	37.58982	7
AATGCGC	30	1.1397471E-4	37.500004	37
CGCTATG	60	1.546141E-10	37.500004	1
CGACTAG	60	1.546141E-10	37.500004	1
CATACGG	230	0.0	37.173916	2
CAACGGG	840	0.0	36.964287	3
CCGAGGG	1775	0.0	36.887325	3
>>END_MODULE