FastQCFastQC Report
Sat 18 Jun 2016
SRR3553121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252841
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT167101.3337686107015974No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC145591.1620788272414457No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGT144231.1512234992309478No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT116080.9265341731313073No Hit
AACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG40560.32374419419543266No Hit
AATGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT35100.28016324497681666No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT32280.2576544030726964No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGGAATC21480.17145032769521432No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTAT20830.1662621194549029No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC18680.14910112296771896No Hit
AATGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC17550.14008162248840833No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16500.13170067071559757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACT207.03353E-445.032
AGTCCGG1450.043.4482732
ATACGCG603.6379788E-1241.2499961
CGTAGCG501.0822987E-940.51
CGCGAGG2350.038.297872
ACGGGAC5850.038.0769235
ACAAGGG13050.037.0689663
CTATGCG552.750312E-936.8181841
CGCTAAG552.750312E-936.8181841
CGGTACG552.750312E-936.81818413
ACCACGG2450.036.7346952
TAGGGCA4350.036.7241365
CCGCAGG2950.036.610172
TATGGGC4800.036.56254
ACGGGTC2900.036.4655155
TAGGGCC4950.036.363645
AATGGGC6250.036.364
TACGGCT20450.036.1980446
AACGGGA9300.036.048394
ATAGGGC5250.036.04
Produced by FastQC (version 0.11.5)