##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1252841
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.694840765907244	31.0	31.0	34.0	30.0	34.0
2	31.898980796445837	33.0	31.0	34.0	30.0	34.0
3	32.02896935844213	33.0	31.0	34.0	30.0	34.0
4	35.69115554168486	37.0	35.0	37.0	33.0	37.0
5	35.452789300477875	37.0	35.0	37.0	33.0	37.0
6	35.464876229306036	37.0	35.0	37.0	33.0	37.0
7	35.56595050768613	37.0	35.0	37.0	33.0	37.0
8	35.67812356077108	37.0	35.0	37.0	35.0	37.0
9	37.404397684941664	39.0	37.0	39.0	35.0	39.0
10	36.92381635019927	39.0	37.0	39.0	33.0	39.0
11	36.925753547337614	39.0	37.0	39.0	33.0	39.0
12	36.846010786684026	39.0	37.0	39.0	33.0	39.0
13	36.88847906478156	39.0	37.0	39.0	33.0	39.0
14	38.00608616735882	40.0	37.0	41.0	33.0	41.0
15	38.038737557279816	40.0	37.0	41.0	33.0	41.0
16	37.89452851558977	40.0	37.0	41.0	33.0	41.0
17	37.85743282667154	40.0	37.0	41.0	33.0	41.0
18	37.766472361616515	40.0	37.0	41.0	33.0	41.0
19	37.81429087968864	40.0	37.0	41.0	33.0	41.0
20	37.85076797454745	40.0	37.0	41.0	33.0	41.0
21	37.88914235725044	40.0	37.0	41.0	33.0	41.0
22	37.88391344153009	40.0	37.0	41.0	33.0	41.0
23	37.768768742402266	40.0	37.0	41.0	33.0	41.0
24	37.6497049505883	39.0	37.0	41.0	32.0	41.0
25	37.70801642027999	39.0	37.0	41.0	33.0	41.0
26	37.66357582486525	39.0	37.0	41.0	33.0	41.0
27	37.62541775053658	40.0	37.0	41.0	33.0	41.0
28	37.591881172471204	40.0	36.0	41.0	33.0	41.0
29	37.43057977827993	39.0	36.0	41.0	32.0	41.0
30	37.452567404802366	39.0	36.0	41.0	32.0	41.0
31	37.41285925348867	39.0	36.0	41.0	32.0	41.0
32	37.351168264767836	39.0	36.0	41.0	32.0	41.0
33	37.3059614109053	39.0	36.0	41.0	32.0	41.0
34	37.191516720796976	39.0	36.0	41.0	31.0	41.0
35	37.161621466730416	39.0	36.0	41.0	31.0	41.0
36	37.07171859797053	39.0	36.0	41.0	31.0	41.0
37	36.97138343971821	39.0	35.0	41.0	31.0	41.0
38	36.956819740094716	39.0	35.0	40.0	31.0	41.0
39	36.77570817047015	39.0	35.0	40.0	31.0	41.0
40	36.66134329895014	39.0	35.0	40.0	30.0	41.0
41	36.52785149911281	39.0	35.0	40.0	30.0	41.0
42	36.53820476820283	39.0	35.0	40.0	30.0	41.0
43	36.5076885255192	39.0	35.0	40.0	30.0	41.0
44	36.43804521084479	39.0	35.0	40.0	30.0	41.0
45	36.27337786678437	38.0	35.0	40.0	30.0	41.0
46	36.021079290987444	38.0	35.0	40.0	30.0	41.0
47	35.92382832298751	38.0	35.0	40.0	29.0	41.0
48	35.806364893869215	38.0	35.0	40.0	29.0	41.0
49	35.69143969585925	38.0	34.0	40.0	28.0	41.0
50	35.61119727084283	38.0	34.0	40.0	28.0	41.0
51	34.21443822480267	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	3.0
12	12.0
13	13.0
14	18.0
15	43.0
16	70.0
17	125.0
18	288.0
19	537.0
20	1069.0
21	1739.0
22	2524.0
23	3517.0
24	4487.0
25	5752.0
26	7266.0
27	9302.0
28	12355.0
29	16564.0
30	22026.0
31	29910.0
32	39219.0
33	53668.0
34	77349.0
35	97619.0
36	121175.0
37	173815.0
38	269135.0
39	303172.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.730629026348915	34.9539965566261	20.96427240168545	25.35110201533954
2	20.87431685265728	32.6131568171859	21.579992991927945	24.932533338228875
3	20.065435278698573	27.33323701890344	26.63171144622502	25.969616256172973
4	21.90317845600519	27.781338573689716	21.164457421173157	29.15102554913193
5	17.32015475227902	35.12177522925894	20.427731851048936	27.130338167413104
6	20.70973092355694	32.29954958370615	24.384658548051988	22.60606094468492
7	79.23607225497888	7.310424866363728	7.723645698057455	5.729857180599932
8	81.89466979449108	3.1019099789997293	5.3662835108365705	9.637136715672618
9	76.01627022104161	6.1484258577105955	10.96076836565853	6.874535555589256
10	32.655141394638264	26.271969068700656	17.125477215384873	23.947412321276204
11	22.380493614113842	25.050984123284596	30.975917933720243	21.59260432888132
12	20.324606234949208	21.30916852178369	39.74398985984654	18.622235383420563
13	23.582801009864777	25.272001794321863	29.326466806242774	21.818730389570582
14	18.211728383729458	27.35207420574518	33.36193499414531	21.07426241638005
15	17.21631076888448	32.606531874355966	27.594403439861882	22.582753916897673
16	17.373872662213323	30.736222713017852	29.544291733747542	22.34561289102129
17	16.539688595759557	30.641318411514312	30.560063088612203	22.25892990411393
18	17.210084918996106	29.928618236472147	29.517233232309607	23.34406361222214
19	17.022990147991646	33.25545699733645	32.48943800530155	17.23211484937035
20	17.982728853860944	32.240004916825036	30.5594245399057	19.217841689408313
21	17.150220977761744	31.267734692590682	29.986486712998694	21.59555761664888
22	16.10459747086821	32.002145523653844	30.411281239997734	21.481975765480215
23	18.94709703785237	29.617565197818397	27.262118656717014	24.17321910761222
24	14.641602565688702	33.934473728110746	28.397138982520527	23.02678472368002
25	17.078464066868822	29.49001509369505	30.133113459728726	23.2984073797074
26	20.272325059604533	31.02827892765323	27.932115887012	20.767280125730238
27	16.004425142536043	30.030386936570565	30.492376925723214	23.472810995170175
28	20.32923571307133	28.245483664726812	31.06052563733147	20.364754984870387
29	21.693335387331672	32.23856818223542	26.80515723862805	19.262939191804865
30	20.755626611836618	31.477577761264197	26.9621604018387	20.804635225060483
31	23.214278587626044	31.715596791612022	25.33258410285104	19.737540517910894
32	22.645012415781412	30.001492607601442	25.64299859279829	21.710496383818857
33	23.312455451250397	28.978537579788654	28.26575758615818	19.443249382802765
34	20.44034318800231	30.577463540864326	28.212039676223878	20.77015359490949
35	21.70730364028636	30.82386352298496	28.966006061423595	18.50282677530509
36	23.469538433049365	30.17174565647197	29.24896295699135	17.109752953487316
37	22.669516722393343	33.2473953199169	24.005679890744318	20.077408066945445
38	25.70413963144565	29.73529761557931	27.28774042356532	17.272822329409717
39	22.78541331262307	29.894056787732843	27.962846043512307	19.357683856131782
40	22.129543972459395	32.98503162013376	25.59750199746017	19.287922409946674
41	18.284842210623694	31.27611564436349	31.191986852282135	19.247055292730682
42	21.36895264442974	30.86081952937364	27.672785293584738	20.097442532611883
43	20.01778358147602	28.211880039047255	29.98784362900001	21.782492750476717
44	20.92635857223702	26.83461029771535	27.649957177327373	24.58907395272026
45	25.196812684131505	27.504767165186962	28.075789345974467	19.222630804707062
46	19.430638045849395	26.821440230643795	33.79335446397428	19.954567259532535
47	21.062848358251365	28.88578838016955	27.40020481449761	22.651158447081475
48	19.515884298167126	27.290853348509508	33.602508219319134	19.590754134004236
49	20.980555393701195	26.459542751234995	30.10014838275567	22.45975347230814
50	19.96246929977547	25.405378655392024	27.969391167753926	26.66276087707858
51	19.852000373550993	24.950572339187495	32.8593173435416	22.338109943719914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2010.0
1	1616.5
2	1223.0
3	879.5
4	536.0
5	574.0
6	612.0
7	665.0
8	718.0
9	868.0
10	1018.0
11	1245.5
12	1473.0
13	1569.0
14	1665.0
15	1886.0
16	2107.0
17	2780.0
18	3453.0
19	4464.0
20	5475.0
21	6922.5
22	8370.0
23	10218.0
24	12066.0
25	14355.5
26	20952.5
27	25260.0
28	29520.5
29	33781.0
30	37282.0
31	40783.0
32	45069.0
33	49355.0
34	52724.5
35	56094.0
36	58205.0
37	60316.0
38	60495.0
39	60674.0
40	63083.5
41	65493.0
42	68494.5
43	71496.0
44	96210.5
45	120925.0
46	124069.5
47	127214.0
48	126787.5
49	126361.0
50	112510.5
51	98660.0
52	84690.0
53	70720.0
54	58573.0
55	46426.0
56	40399.0
57	34372.0
58	29718.5
59	25065.0
60	24196.0
61	23327.0
62	19829.0
63	16331.0
64	14711.5
65	13092.0
66	11186.0
67	9280.0
68	7886.0
69	6492.0
70	5556.5
71	4621.0
72	3963.5
73	3306.0
74	2836.0
75	2050.0
76	1734.0
77	1305.5
78	877.0
79	739.0
80	601.0
81	447.5
82	294.0
83	187.0
84	80.0
85	53.5
86	27.0
87	28.5
88	30.0
89	20.5
90	11.0
91	6.5
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1252841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.914770793073274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90930588710638	21.37070639200822
2	10.57550091702759	6.11576370075578
3	4.103603302452281	3.5596424674831866
4	2.0789409659517153	2.404484060912968
5	1.3356588757158294	1.9310135124528574
6	0.9824077683955155	1.7043657269094565
7	0.6910701947484326	1.3987495398152805
8	0.5591066108122859	1.2933151600423414
9	0.45544581938623757	1.1852200328594857
>10	4.350338525719815	27.05722949160707
>50	0.695559032909408	13.938771205909797
>100	0.2541447404620482	10.604212731692446
>500	0.005016014613270747	1.0167893350530697
>1k	0.002786674785150415	1.8027282505065638
>5k	0.0	0.0
>10k+	0.0011146699140601659	4.617008391991512
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	16710	1.3337686107015974	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC	14559	1.1620788272414457	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGT	14423	1.1512234992309478	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	11608	0.9265341731313073	No Hit
AACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG	4056	0.32374419419543266	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT	3510	0.28016324497681666	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT	3228	0.2576544030726964	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGGAATC	2148	0.17145032769521432	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTAT	2083	0.1662621194549029	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	1868	0.14910112296771896	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	1755	0.14008162248840833	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1650	0.13170067071559757	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1927435324993357E-4	0.0	0.0	1.0614275873794041	0.0
2	3.1927435324993357E-4	0.0	0.0	1.455571776466447	0.0
3	3.1927435324993357E-4	0.0	0.0	2.2166420160259763	0.0
4	3.1927435324993357E-4	0.0	0.0	5.355747457179323	0.0
5	3.1927435324993357E-4	0.0	0.0	5.77543359452636	0.0
6	3.1927435324993357E-4	0.0	0.0	7.513722810795624	0.0
7	3.1927435324993357E-4	0.0	0.0	8.83488008454385	0.0
8	3.1927435324993357E-4	0.0	0.0	9.818484548318581	0.0
9	3.1927435324993357E-4	0.0	0.0	12.263966457036448	0.0
10	3.1927435324993357E-4	0.0	0.0	14.34611415175589	0.0
11	3.1927435324993357E-4	0.0	0.0	17.37443139233151	0.0
12	3.1927435324993357E-4	0.0	0.0	18.302162844287505	0.0
13	3.1927435324993357E-4	0.0	0.0	18.76207755014403	0.0
14	3.1927435324993357E-4	0.0	0.0	19.27243760381405	0.0
15	3.1927435324993357E-4	0.0	0.0	19.618371365560353	0.0
16	3.1927435324993357E-4	0.0	0.0	20.34328378461433	0.0
17	3.1927435324993357E-4	0.0	0.0	21.369671011724552	0.0
18	3.1927435324993357E-4	0.0	0.0	22.709106742196337	0.0
19	3.1927435324993357E-4	0.0	0.0	23.25881735990441	0.0
20	3.1927435324993357E-4	0.0	0.0	23.805255415491672	0.0
21	3.1927435324993357E-4	0.0	0.0	24.417463987848418	0.0
22	3.1927435324993357E-4	0.0	0.0	25.06678820377047	0.0
23	3.1927435324993357E-4	0.0	0.0	25.721699720874398	0.0
24	3.1927435324993357E-4	0.0	0.0	26.255127346566724	0.0
25	3.1927435324993357E-4	0.0	0.0	26.735395792442937	0.0
26	3.1927435324993357E-4	0.0	0.0	27.163063788621223	0.0
27	3.1927435324993357E-4	0.0	0.0	27.614517724116627	0.0
28	3.1927435324993357E-4	0.0	0.0	28.064135832080847	0.0
29	3.1927435324993357E-4	0.0	0.0	28.518622873932127	0.0
30	3.1927435324993357E-4	0.0	0.0	29.13945185382662	0.0
31	3.1927435324993357E-4	0.0	0.0	29.586276311199907	0.0
32	3.1927435324993357E-4	0.0	0.0	30.001811881954694	0.0
33	3.1927435324993357E-4	0.0	0.0	30.43139552425248	0.0
34	3.1927435324993357E-4	0.0	0.0	30.8138861994459	0.0
35	3.1927435324993357E-4	0.0	0.0	31.23405124832281	0.0
36	3.1927435324993357E-4	0.0	0.0	31.641684778834666	0.0
37	3.1927435324993357E-4	0.0	0.0	32.02369654249821	0.0
38	3.9909294156241694E-4	0.0	0.0	32.410577240048816	0.0
39	3.9909294156241694E-4	0.0	0.0	32.82459625762567	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCACT	20	7.03353E-4	45.0	32
AGTCCGG	145	0.0	43.448273	2
ATACGCG	60	3.6379788E-12	41.249996	1
CGTAGCG	50	1.0822987E-9	40.5	1
CGCGAGG	235	0.0	38.29787	2
ACGGGAC	585	0.0	38.076923	5
ACAAGGG	1305	0.0	37.068966	3
CTATGCG	55	2.750312E-9	36.818184	1
CGCTAAG	55	2.750312E-9	36.818184	1
CGGTACG	55	2.750312E-9	36.818184	13
ACCACGG	245	0.0	36.734695	2
TAGGGCA	435	0.0	36.724136	5
CCGCAGG	295	0.0	36.61017	2
TATGGGC	480	0.0	36.5625	4
ACGGGTC	290	0.0	36.465515	5
TAGGGCC	495	0.0	36.36364	5
AATGGGC	625	0.0	36.36	4
TACGGCT	2045	0.0	36.198044	6
AACGGGA	930	0.0	36.04839	4
ATAGGGC	525	0.0	36.0	4
>>END_MODULE