FastQCFastQC Report
Sat 18 Jun 2016
SRR3553119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286408
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT85090.6614542198120658No Hit
AATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT58700.45630935131000433No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGT54290.42202784808552185No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCC51150.3976187959030105No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC19190.14917506731923308No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15970.12414412845691258No Hit
AACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG14610.1135720549001561No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTT14530.11295016822034688No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT13920.10820828228680171No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAC253.8910654E-545.00000411
CCGAACG356.248869E-638.571431
CGGGTAG2150.037.674426
GCGGGAC6100.037.622955
CGTATCT301.1399977E-437.49999620
CGCCTAT301.1399977E-437.49999613
TCGTGTC301.1399977E-437.49999644
AGGGCGT3900.036.9230776
ACGGGCG4350.036.7241365
CTAACCG800.036.56251
TAGGGAC6250.036.365
TAACCGG2600.036.3461532
ACACGGG10650.036.338033
CACGGGC8300.036.32534
ACGGGCC5600.036.1607175
CCCATAG504.879803E-836.0000041
TAACTCG250.002107201436.0000041
ACGGGAT12250.036.05
TACGGGA8200.035.9451184
ACGGGTA4150.035.783135