##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553119_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1286408
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.693948576190447	31.0	31.0	34.0	30.0	34.0
2	31.891577166808663	33.0	31.0	34.0	30.0	34.0
3	32.050223568261394	33.0	31.0	34.0	30.0	34.0
4	35.72490298567795	37.0	35.0	37.0	33.0	37.0
5	35.50998672271938	37.0	35.0	37.0	33.0	37.0
6	35.53858962319886	37.0	35.0	37.0	33.0	37.0
7	35.69838651500923	37.0	35.0	37.0	35.0	37.0
8	35.7653232877905	37.0	35.0	37.0	35.0	37.0
9	37.47726615506122	39.0	37.0	39.0	35.0	39.0
10	36.964036293306634	39.0	37.0	39.0	33.0	39.0
11	36.93744519623634	39.0	37.0	39.0	33.0	39.0
12	36.881043183811045	39.0	37.0	39.0	33.0	39.0
13	36.87202582695381	39.0	37.0	39.0	33.0	39.0
14	38.02874671177418	40.0	37.0	41.0	33.0	41.0
15	38.019419188935395	40.0	37.0	41.0	33.0	41.0
16	37.93209308399823	40.0	37.0	41.0	33.0	41.0
17	37.890778819783456	40.0	37.0	41.0	33.0	41.0
18	37.827942612297186	40.0	37.0	41.0	33.0	41.0
19	37.87946670107773	40.0	37.0	41.0	33.0	41.0
20	37.83125804565892	40.0	37.0	41.0	33.0	41.0
21	37.85472338480482	40.0	37.0	41.0	33.0	41.0
22	37.82427037145291	40.0	37.0	41.0	33.0	41.0
23	37.70091448436266	39.0	37.0	41.0	33.0	41.0
24	37.57512857507105	39.0	36.0	41.0	32.0	41.0
25	37.6429087816618	39.0	36.0	41.0	32.0	41.0
26	37.542264973476534	39.0	36.0	41.0	32.0	41.0
27	37.531021262305586	39.0	36.0	41.0	32.0	41.0
28	37.481674554262725	39.0	36.0	41.0	32.0	41.0
29	37.18569069844093	39.0	36.0	41.0	31.0	41.0
30	37.28320641662676	39.0	36.0	41.0	32.0	41.0
31	37.16996085223351	39.0	36.0	41.0	31.0	41.0
32	37.04910261752104	39.0	36.0	41.0	31.0	41.0
33	37.09414042823117	39.0	36.0	41.0	31.0	41.0
34	36.925572602160436	39.0	35.0	41.0	31.0	41.0
35	36.85860939919528	39.0	35.0	41.0	31.0	41.0
36	36.753934987966495	39.0	35.0	40.0	30.0	41.0
37	36.65669134520308	39.0	35.0	40.0	30.0	41.0
38	36.646430214986225	39.0	35.0	40.0	30.0	41.0
39	36.52662063668758	39.0	35.0	40.0	30.0	41.0
40	36.466912519200754	39.0	35.0	40.0	30.0	41.0
41	36.450800212685245	39.0	35.0	40.0	30.0	41.0
42	36.35866459163811	39.0	35.0	40.0	30.0	41.0
43	36.26036762831077	39.0	35.0	40.0	30.0	41.0
44	36.18826919608709	38.0	35.0	40.0	30.0	41.0
45	35.95642828713752	38.0	35.0	40.0	29.0	41.0
46	35.79360202983812	38.0	34.0	40.0	28.0	41.0
47	35.68077079744529	38.0	34.0	40.0	28.0	41.0
48	35.62631995447789	38.0	34.0	40.0	28.0	41.0
49	35.468094881250735	38.0	34.0	40.0	28.0	41.0
50	35.393952773925534	38.0	34.0	40.0	28.0	41.0
51	34.05211021697626	36.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	11.0
12	11.0
13	19.0
14	31.0
15	42.0
16	104.0
17	181.0
18	374.0
19	655.0
20	1209.0
21	1908.0
22	2728.0
23	3930.0
24	5268.0
25	6790.0
26	9054.0
27	11285.0
28	14583.0
29	18820.0
30	24487.0
31	31934.0
32	41032.0
33	54682.0
34	80559.0
35	98540.0
36	120928.0
37	178276.0
38	273035.0
39	305851.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.34642197498772	33.101162306204564	20.81851170079788	25.73390401800984
2	21.21613049670089	30.860038183842143	21.91505338897146	26.008777930485504
3	20.521716282858936	28.939729852426293	23.588939123512915	26.94961474120186
4	20.42003781071013	28.812398554735356	21.286403691519332	29.48115994303518
5	17.774298667296843	33.168403803458936	20.54651401421633	28.510783515027892
6	20.940012810865603	33.08273891331522	21.816484350221703	24.16076392559748
7	83.02334873539343	5.135151522689536	6.714199538560084	5.127300203356945
8	85.56974148170721	3.0834696301639917	4.69563311173438	6.651155776394425
9	77.83611420326989	6.576607110652296	9.63279146273966	5.954487223338163
10	34.04021119271646	27.21189544841139	16.240181964042513	22.507711394829634
11	23.20531277790561	25.305968246466126	30.95130005410414	20.53741892152412
12	21.8213817078252	20.517129868595344	38.269895709603794	19.39159271397566
13	23.68191118214439	23.732672682383814	30.631261621507328	21.954154513964465
14	19.73448548205546	26.93554455507117	31.630711251795695	21.69925871107767
15	18.43287666121479	32.21987114507994	28.3119352491589	21.035316944546363
16	19.089278051753407	28.919829478672398	29.383135055130253	22.60775741444394
17	18.13483746991623	29.44073730884758	31.252215471296818	21.172209749939366
18	18.092860119029112	29.22851847936269	30.24017263574231	22.438448765865886
19	18.26830989857028	33.1675487092742	30.973143823732443	17.59099756842308
20	19.40869459767041	31.13607813384245	29.956670045584293	19.498557222902843
21	18.798934708117486	30.608951436869177	29.661429344344874	20.930684510668467
22	17.049334270309263	30.820004228829422	30.205424717507977	21.925236783353338
23	18.927820722507946	29.63041274618939	28.176752632135372	23.265013899167293
24	16.221758571153163	31.849459891418586	29.112381141908322	22.816400395519928
25	18.603662290657397	29.61844142760306	28.85655250900181	22.921343772737732
26	19.623556445544494	30.432957506483167	29.219112443330573	20.724373604641762
27	18.072959745275217	28.711497440936313	29.378470905031683	23.837071908756784
28	21.583587788633153	27.882444760915664	29.404123730573815	21.129843719877364
29	19.53517080117661	29.856235346795106	30.163058687445975	20.44553516458231
30	22.713711357516434	28.683357068674947	27.79025006063395	20.81268151317467
31	21.554436850517096	29.70426178941673	27.27447279556719	21.46682856449898
32	21.14974409363126	27.177847152691836	30.471436744796364	21.20097200888054
33	22.46884347734156	26.29150316229377	30.853819317044046	20.385834043320624
34	19.802426601824617	25.786064763278837	30.88957780113308	23.52193083376347
35	21.280884447236026	24.981343399605723	33.745903321496755	19.991868831661495
36	21.16645729815113	26.436169551184385	32.91078724634797	19.486585904316517
37	21.99395526147225	27.193005640512187	30.450448069352802	20.362591028662756
38	21.6440662682446	26.923184557309966	32.41724243008439	19.015506744361044
39	18.887009409145467	26.089389991355777	33.29845585537403	21.725144744124727
40	19.49777986455308	27.015146050086752	31.182253219818286	22.30482086554188
41	18.00882768141989	25.281170515108737	34.36607981293648	22.343921990534884
42	20.822476228381664	26.856176267560528	31.074433616706365	21.246913887351447
43	19.07994975155627	26.134787718981844	32.034315706991876	22.75094682247001
44	22.094545431931394	24.749535139706843	29.297081485811656	23.85883794255011
45	22.924142262796874	25.766630804534795	30.765278201006215	20.543948731662116
46	18.2323959428113	26.703347615997412	34.18215682738291	20.88209961380837
47	19.41514667197343	26.72806761151983	31.356303754329883	22.500481962176853
48	19.888480171143215	24.491607639255975	34.10387684156193	21.51603534803888
49	20.002596376888203	24.891092095198413	31.966374587222717	23.139936940690667
50	19.433259121522877	24.145916381117033	30.112763602216404	26.308060895143687
51	19.20961312429649	23.70072325420862	33.830946324960664	23.258717296534225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2110.0
1	1613.0
2	1116.0
3	825.5
4	535.0
5	635.5
6	736.0
7	760.5
8	785.0
9	926.0
10	1067.0
11	1346.5
12	1626.0
13	1704.0
14	1782.0
15	2263.5
16	2745.0
17	3230.0
18	3715.0
19	4242.5
20	4770.0
21	6251.5
22	7733.0
23	10226.0
24	12719.0
25	15892.0
26	20962.5
27	22860.0
28	28066.0
29	33272.0
30	36898.5
31	40525.0
32	45200.5
33	49876.0
34	52135.5
35	54395.0
36	57895.5
37	61396.0
38	63411.0
39	65426.0
40	66102.5
41	66779.0
42	69114.5
43	71450.0
44	84063.0
45	96676.0
46	105209.0
47	113742.0
48	120920.0
49	128098.0
50	117751.5
51	107405.0
52	93731.0
53	80057.0
54	67403.0
55	54749.0
56	48204.0
57	41659.0
58	36932.0
59	32205.0
60	30908.5
61	29612.0
62	27343.5
63	25075.0
64	20696.5
65	16318.0
66	13760.5
67	11203.0
68	9058.0
69	6913.0
70	6003.0
71	5093.0
72	4759.0
73	4425.0
74	3683.0
75	2290.5
76	1640.0
77	1373.5
78	1107.0
79	773.5
80	440.0
81	395.0
82	350.0
83	217.0
84	84.0
85	64.5
86	45.0
87	31.5
88	18.0
89	34.5
90	51.0
91	30.5
92	10.0
93	8.0
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1286408.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.43733125613157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76889153577046	22.45328188071886
2	10.798919148949281	6.573805586895035
3	4.010192913921436	3.661787103660548
4	2.122679669425746	2.5843481699586914
5	1.3169863558654582	2.004277498664126
6	0.9184536495016372	1.6773166783970574
7	0.6928579323049039	1.4762122479301918
8	0.5465615773606014	1.3308700625598724
9	0.47582397682773236	1.3034530802084006
>10	4.395621795496156	29.15420798306732
>50	0.7595756802951755	16.04506049671712
>100	0.1862141624333045	8.042476574194916
>500	0.003868715275776396	0.7871420165273375
>1k	0.002321229165465838	0.9492491536699477
>5k	0.0010316574068737056	1.9565114668306405
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	8509	0.6614542198120658	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT	5870	0.45630935131000433	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGT	5429	0.42202784808552185	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCC	5115	0.3976187959030105	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	1919	0.14917506731923308	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1597	0.12414412845691258	No Hit
AACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG	1461	0.1135720549001561	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTT	1453	0.11295016822034688	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT	1392	0.10820828228680171	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7822557073650039	0.0
2	0.0	0.0	0.0	1.1312896064079203	0.0
3	0.0	0.0	0.0	1.6190819708832656	0.0
4	0.0	0.0	0.0	3.768477807973831	0.0
5	0.0	0.0	0.0	4.096756239078116	0.0
6	0.0	0.0	0.0	5.7834683863906315	0.0
7	0.0	0.0	0.0	7.008274202274862	0.0
8	0.0	0.0	0.0	7.939860448629051	0.0
9	0.0	0.0	0.0	9.68347522714411	0.0
10	0.0	0.0	0.0	11.876714075161225	0.0
11	0.0	0.0	0.0	14.320806462646377	0.0
12	0.0	0.0	0.0	15.29110515481869	0.0
13	0.0	0.0	0.0	15.706369985261286	0.0
14	0.0	0.0	0.0	16.14347858533218	0.0
15	0.0	0.0	0.0	16.484117014197672	0.0
16	0.0	0.0	0.0	17.205661034446305	0.0
17	0.0	0.0	0.0	18.25043065652577	0.0
18	0.0	0.0	0.0	19.429061386434164	0.0
19	0.0	0.0	0.0	19.99070279413685	0.0
20	0.0	0.0	0.0	20.53726344985417	0.0
21	0.0	0.0	0.0	21.16521352479151	0.0
22	0.0	0.0	0.0	21.855196796039827	0.0
23	0.0	0.0	0.0	22.545646482297997	0.0
24	0.0	0.0	0.0	23.10697694666078	0.0
25	7.773583497615064E-5	0.0	0.0	23.606896101392405	0.0
26	7.773583497615064E-5	0.0	0.0	24.037241683820373	0.0
27	7.773583497615064E-5	0.0	0.0	24.52099178487696	0.0
28	7.773583497615064E-5	0.0	0.0	24.977534343691893	0.0
29	7.773583497615064E-5	0.0	0.0	25.463538783962786	0.0
30	2.3320750492845195E-4	0.0	0.0	26.107191497565314	0.0
31	2.3320750492845195E-4	0.0	0.0	26.546554436850517	0.0
32	2.3320750492845195E-4	0.0	0.0	26.97239134084987	0.0
33	2.3320750492845195E-4	0.0	0.0	27.39511881145018	0.0
34	2.3320750492845195E-4	0.0	0.0	27.82258816798403	0.0
35	2.3320750492845195E-4	0.0	0.0	28.26482733316335	0.0
36	2.3320750492845195E-4	0.0	0.0	28.69113065217256	0.0
37	2.3320750492845195E-4	0.0	0.0	29.105929067605302	0.0
38	2.3320750492845195E-4	0.0	0.0	29.551122194513717	0.0
39	2.3320750492845195E-4	0.0	0.0	29.99755909478175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAC	25	3.8910654E-5	45.000004	11
CCGAACG	35	6.248869E-6	38.57143	1
CGGGTAG	215	0.0	37.67442	6
GCGGGAC	610	0.0	37.62295	5
CGTATCT	30	1.1399977E-4	37.499996	20
CGCCTAT	30	1.1399977E-4	37.499996	13
TCGTGTC	30	1.1399977E-4	37.499996	44
AGGGCGT	390	0.0	36.923077	6
ACGGGCG	435	0.0	36.724136	5
CTAACCG	80	0.0	36.5625	1
TAGGGAC	625	0.0	36.36	5
TAACCGG	260	0.0	36.346153	2
ACACGGG	1065	0.0	36.33803	3
CACGGGC	830	0.0	36.3253	4
ACGGGCC	560	0.0	36.160717	5
CCCATAG	50	4.879803E-8	36.000004	1
TAACTCG	25	0.0021072014	36.000004	1
ACGGGAT	1225	0.0	36.0	5
TACGGGA	820	0.0	35.945118	4
ACGGGTA	415	0.0	35.78313	5
>>END_MODULE