##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1082968
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.644778977772198	31.0	31.0	34.0	30.0	34.0
2	31.85959141913704	33.0	31.0	34.0	30.0	34.0
3	32.007482215540996	33.0	31.0	34.0	30.0	34.0
4	35.69521444770298	37.0	35.0	37.0	33.0	37.0
5	35.45580017138087	37.0	35.0	37.0	33.0	37.0
6	35.48319710277681	37.0	35.0	37.0	33.0	37.0
7	35.636883084264724	37.0	35.0	37.0	35.0	37.0
8	35.72956726329864	37.0	35.0	37.0	35.0	37.0
9	37.427934158719374	39.0	37.0	39.0	35.0	39.0
10	36.892632099932776	39.0	37.0	39.0	32.0	39.0
11	36.89535147852938	39.0	37.0	39.0	33.0	39.0
12	36.86452785308523	39.0	37.0	39.0	33.0	39.0
13	36.86265245141131	39.0	37.0	39.0	33.0	39.0
14	38.00857089036795	40.0	37.0	41.0	33.0	41.0
15	38.01154697091696	40.0	37.0	41.0	33.0	41.0
16	37.89231353096306	40.0	37.0	41.0	33.0	41.0
17	37.87511727031639	40.0	37.0	41.0	33.0	41.0
18	37.80577911812722	40.0	37.0	41.0	33.0	41.0
19	37.865784584586066	40.0	37.0	41.0	33.0	41.0
20	37.83686313907705	40.0	37.0	41.0	33.0	41.0
21	37.84275989687599	40.0	37.0	41.0	33.0	41.0
22	37.82440847744347	40.0	37.0	41.0	33.0	41.0
23	37.69580541622652	39.0	37.0	41.0	33.0	41.0
24	37.57768373580752	39.0	36.0	41.0	32.0	41.0
25	37.6346281699921	39.0	36.0	41.0	32.0	41.0
26	37.56821254182949	39.0	36.0	41.0	32.0	41.0
27	37.534060101498845	39.0	36.0	41.0	32.0	41.0
28	37.46420762201653	39.0	36.0	41.0	32.0	41.0
29	37.29625252085011	39.0	36.0	41.0	32.0	41.0
30	37.34912573594049	39.0	36.0	41.0	32.0	41.0
31	37.2259494278686	39.0	36.0	41.0	31.0	41.0
32	37.16261052958167	39.0	36.0	41.0	31.0	41.0
33	37.14618345140392	39.0	36.0	41.0	31.0	41.0
34	37.06339153142106	39.0	36.0	41.0	31.0	41.0
35	37.04275103234814	39.0	36.0	41.0	31.0	41.0
36	36.922801043059444	39.0	35.0	40.0	31.0	41.0
37	36.79876598385179	39.0	35.0	40.0	31.0	41.0
38	36.71044019767897	39.0	35.0	40.0	31.0	41.0
39	36.63615176071684	39.0	35.0	40.0	30.0	41.0
40	36.597814524528886	39.0	35.0	40.0	30.0	41.0
41	36.52102739877817	39.0	35.0	40.0	30.0	41.0
42	36.497498541046454	39.0	35.0	40.0	30.0	41.0
43	36.449040968892895	39.0	35.0	40.0	30.0	41.0
44	36.35246655487512	39.0	35.0	40.0	30.0	41.0
45	36.15187521699625	38.0	35.0	40.0	29.0	41.0
46	35.985053113296054	38.0	35.0	40.0	29.0	41.0
47	35.88971511623612	38.0	35.0	40.0	29.0	41.0
48	35.81425859305169	38.0	34.0	40.0	29.0	41.0
49	35.644883320652134	38.0	34.0	40.0	28.0	41.0
50	35.571833147424485	38.0	34.0	40.0	28.0	41.0
51	34.28140720686115	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	4.0
11	4.0
12	11.0
13	12.0
14	21.0
15	43.0
16	88.0
17	162.0
18	284.0
19	497.0
20	933.0
21	1440.0
22	2163.0
23	3049.0
24	3836.0
25	5054.0
26	6514.0
27	8540.0
28	11210.0
29	14940.0
30	19677.0
31	26427.0
32	34642.0
33	46337.0
34	66942.0
35	85198.0
36	107661.0
37	154575.0
38	230653.0
39	251989.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.37277925109514	33.37688648233373	20.894984893367116	26.355349373204014
2	21.968054457749446	30.619094931706197	21.317804404192923	26.095046206351434
3	20.296259907956653	28.73547510175739	23.767922967252957	27.200342023033
4	21.00542213620347	29.423491737521328	20.561364694062984	29.009721432212217
5	17.635054775395027	34.46020565704619	19.909821896861217	27.994917670697568
6	20.42322580168574	33.50283664891299	22.380162664086104	23.69377488531517
7	82.70179728302222	5.529803281352727	6.6298357846215215	5.138563651003539
8	84.90601753699094	3.088456907313974	4.8755826580286765	7.129942897666414
9	77.43709878777581	6.906667602366828	9.795026261163766	5.86120734869359
10	32.71537109129725	30.742551949826773	15.137012358629248	21.405064600246728
11	21.881440633518256	25.92717421013363	31.543868332212956	20.647516824135153
12	21.87553094828287	21.344952020742994	37.64367922228543	19.135837808688716
13	23.47031491235198	24.715134703887834	30.149551971988092	21.664998411772093
14	19.759863634013193	27.05841723855183	31.561966743246337	21.619752384188637
15	18.18299340331386	31.349033397108688	28.410811769138146	22.05716143043931
16	19.315252158881886	29.830521308108825	28.05604597735113	22.798180555658153
17	17.655184640728073	30.10144344061874	29.776041397345075	22.46733052130811
18	19.16557091252927	29.14056555687703	28.79641873074735	22.897444799846348
19	18.10782959422624	32.760155424721695	30.033666738075365	19.09834824297671
20	19.60990537116517	31.01707529677701	29.182672064179183	20.190347267878643
21	19.264835156717467	30.972290963352567	28.48265138028086	21.280222499649113
22	18.377181966595504	30.111785389780678	28.74738681105998	22.763645832563842
23	19.14350193172836	29.943821054731075	26.704020802092028	24.208656211448538
24	16.46669153659203	32.204183318436	28.290955965457893	23.038169179514075
25	18.43517075296777	29.946129525526143	27.476712146619292	24.14198757488679
26	21.139498119981383	29.608631095286285	27.153249218813485	22.098621565918844
27	18.526678535284514	28.04081007010364	28.300466865133593	25.132044529478247
28	20.11509111995922	29.05376705498223	27.7473572626338	23.083784562424743
29	21.493525201113975	30.936555835444814	25.720242888063176	21.849676075378035
30	21.593528155956594	28.761052958166815	25.795129680655382	23.850289205221205
31	20.93072002127487	32.50400750530025	23.647605469413683	22.9176670040112
32	20.854817501532825	30.828057708076322	24.845794150889038	23.471330639501815
33	20.175388377126563	28.45227190461768	25.38911583721772	25.983223881038036
34	18.679406963086628	31.496498511498032	25.474898611962683	24.34919591345266
35	21.194809080231362	29.8825080704139	25.31940001920648	23.60328283014826
36	22.039524713564944	30.96915144307126	26.558494803170547	20.432829040193244
37	20.06033419270006	34.18485126060973	23.82609643128883	21.928718115401377
38	19.376749820862667	33.905249277910336	25.767797386441703	20.950203514785294
39	18.197398261075122	31.776285171861034	26.4512894194473	23.575027147616552
40	19.058457867637824	32.05376336142896	25.165101831263716	23.7226769396695
41	17.768392048518518	30.03634456419765	29.222377761854457	22.972885625429377
42	21.05020646962791	30.274948105576527	25.838528931602777	22.836316493192783
43	20.15017987604435	27.599615131749044	28.26427004306683	23.98593494913977
44	21.43867593502301	26.86598311307444	26.88759039971633	24.807750552186214
45	22.428640550782664	27.719470935429303	27.784292795354986	22.067595718433047
46	17.806158630725932	27.99445597653855	30.96074860937719	23.238636783358327
47	19.427074484195288	29.147213952766844	26.564404488405934	24.861307074631938
48	19.59974809966684	27.832216649060733	30.50136292115741	22.06667233011502
49	20.555639686491197	26.992302634980902	28.100922649607373	24.351135028920524
50	18.927521404141213	26.02126747974086	27.501366614710683	27.549844501407243
51	19.260402892790925	25.180060722015796	31.478861794623665	24.080674590569622
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1738.0
1	1377.0
2	1016.0
3	757.0
4	498.0
5	533.5
6	569.0
7	618.5
8	668.0
9	795.0
10	922.0
11	1164.5
12	1407.0
13	1559.0
14	1711.0
15	1922.0
16	2133.0
17	2517.0
18	2901.0
19	3539.0
20	4177.0
21	5154.5
22	6132.0
23	7747.5
24	9363.0
25	10801.5
26	14091.0
27	15942.0
28	19718.0
29	23494.0
30	26138.5
31	28783.0
32	33336.5
33	37890.0
34	40171.0
35	42452.0
36	44434.0
37	46416.0
38	47469.0
39	48522.0
40	50991.5
41	53461.0
42	55353.0
43	57245.0
44	72054.5
45	86864.0
46	93411.0
47	99958.0
48	106141.0
49	112324.0
50	104797.0
51	97270.0
52	84526.0
53	71782.0
54	61954.5
55	52127.0
56	44968.5
57	37810.0
58	34194.5
59	30579.0
60	27985.5
61	25392.0
62	22097.0
63	18802.0
64	17672.0
65	16542.0
66	13099.0
67	9656.0
68	8300.5
69	6945.0
70	6378.0
71	5811.0
72	4883.5
73	3956.0
74	3319.0
75	2336.0
76	1990.0
77	1668.5
78	1347.0
79	1072.5
80	798.0
81	579.0
82	360.0
83	233.5
84	107.0
85	104.0
86	101.0
87	68.5
88	36.0
89	29.0
90	22.0
91	19.0
92	16.0
93	10.5
94	5.0
95	4.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1082968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.734196296137274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24273559348205	22.81790809292295
2	10.547865018254885	6.483603079524104
3	3.9887226966186553	3.677705589862072
4	2.05036677673323	2.520654999807894
5	1.2671787844604316	1.9472860751953764
6	0.9085879887524995	1.6754832959179127
7	0.6950376400032863	1.4952996282745494
8	0.5353594352653521	1.3163073577947622
9	0.4532289238016885	1.2536664040087322
>10	4.398820563032017	29.14295413477538
>50	0.7413624765738972	15.847836515069918
>100	0.16346882204249955	7.403544332903908
>500	0.003935360530592624	0.9222913599909838
>1k	0.0021190402857037207	0.9390383028739029
>5k	0.001210880163259269	2.5564208310776015
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	7887	0.728276366430033	No Hit
AATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	7359	0.6795214632380643	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT	6372	0.5883830362485318	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	5859	0.5410132155336076	No Hit
AACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	1725	0.15928448486012514	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1664	0.15365181612014392	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	1642	0.15162036182047853	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT	1560	0.1440485776126349	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1395	0.12881267036514465	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.821076892392019	0.0
2	0.0	0.0	0.0	1.2018822347474718	0.0
3	0.0	0.0	0.0	1.785463651742249	0.0
4	0.0	0.0	0.0	4.159587356228439	0.0
5	0.0	0.0	0.0	4.508074107452852	0.0
6	0.0	0.0	0.0	6.139701265411351	0.0
7	0.0	0.0	0.0	7.360605299510235	0.0
8	0.0	0.0	0.0	8.41280628790509	0.0
9	0.0	0.0	0.0	10.308522504820086	0.0
10	0.0	0.0	0.0	12.452722518116879	0.0
11	0.0	0.0	0.0	14.763871139313443	0.0
12	0.0	0.0	0.0	15.662512650419957	0.0
13	0.0	0.0	0.0	16.056614784555038	0.0
14	0.0	0.0	0.0	16.470477428695954	0.0
15	0.0	0.0	0.0	16.7858145393031	0.0
16	0.0	0.0	0.0	17.469768266467707	0.0
17	0.0	0.0	0.0	18.43738688493104	0.0
18	0.0	0.0	0.0	19.59042187765474	0.0
19	0.0	0.0	0.0	20.134759291133257	0.0
20	0.0	0.0	0.0	20.666076929327552	0.0
21	0.0	0.0	0.0	21.263878526419987	0.0
22	0.0	0.0	0.0	21.92539391745647	0.0
23	0.0	0.0	0.0	22.608885950462064	0.0
24	0.0	0.0	0.0	23.140388266319967	0.0
25	0.0	0.0	0.0	23.608361465897424	0.0
26	0.0	0.0	0.0	24.013636598680662	0.0
27	0.0	0.0	0.0	24.46785131231948	0.0
28	0.0	0.0	0.0	24.905999069224574	0.0
29	0.0	0.0	0.0	25.366585162257795	0.0
30	0.0	0.0	0.0	26.010648514083517	0.0
31	0.0	0.0	0.0	26.452489824260734	0.0
32	0.0	0.0	0.0	26.881957730976353	0.0
33	0.0	0.0	0.0	27.31798169474998	0.0
34	0.0	0.0	0.0	27.73406047085417	0.0
35	0.0	0.0	0.0	28.161773939765535	0.0
36	0.0	0.0	0.0	28.584501111759536	0.0
37	0.0	0.0	0.0	29.015077084456788	0.0
38	0.0	0.0	0.0	29.43032481107475	0.0
39	0.0	0.0	0.0	29.875397980365072	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGCAT	35	1.2118471E-7	45.0	5
CTAGCGT	25	3.8906557E-5	45.0	29
TAGCGCG	120	0.0	43.124996	1
GTACTAG	55	6.184564E-11	40.909092	1
TACGCGG	145	0.0	40.34483	2
CGCATAG	45	1.9281288E-8	40.0	1
AGTACGG	110	0.0	38.86364	2
TTAGCGG	225	0.0	38.0	2
GTAGGGC	285	0.0	37.894737	4
CTAACGG	190	0.0	37.894737	2
TGCGGGA	745	0.0	37.75168	4
TAGGGCG	155	0.0	37.74194	5
GTAACGG	155	0.0	37.74194	2
TGACGGG	455	0.0	37.582417	3
TACTACG	90	0.0	37.5	1
CGGCACG	30	1.1398784E-4	37.499996	1
CGTGTTC	30	1.1398784E-4	37.499996	33
TATAGCG	60	1.5643309E-10	37.499996	1
TATCGGC	30	1.1398784E-4	37.499996	32
AACGGGA	1275	0.0	37.411766	4
>>END_MODULE