FastQCFastQC Report
Sat 18 Jun 2016
SRR3553115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758329
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT51720.6820258753126941Illumina Single End Adapter 1 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT12870.16971525551574582No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11360.1498030538196482No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC11050.145715118372105No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC10030.13226449206083377No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGT9820.12949524546733673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA207.0317666E-445.09
CGACTAG253.8895465E-545.01
CGATACG351.2111923E-745.01
TGTAGCG2250.041.0000041
CATTGCG403.4561526E-739.3751
CTAGGGA6500.039.1153834
TAGGGAA7000.038.8928575
TACACGC4650.038.7096835
CACTAGG1700.038.3823552
CACGGGC5050.038.316834
AGACGGG3300.038.1818163
TAGGGAC3250.038.076925
ACGGGAG7050.037.978725
TCGCCGA301.13955546E-437.50000444
GCGACGG1500.037.5000042
TAGCCGT301.13955546E-437.50000444
AACATAG601.546141E-1037.5000041
CGCTACG301.13955546E-437.5000041
CGCTAAG301.13955546E-437.5000041
ATAGGGC5100.037.54
Produced by FastQC (version 0.11.5)