##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	758329
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69983608697544	31.0	31.0	34.0	30.0	34.0
2	31.912661918507666	33.0	31.0	34.0	30.0	34.0
3	32.06468168829097	34.0	31.0	34.0	30.0	34.0
4	35.742734354086416	37.0	35.0	37.0	33.0	37.0
5	35.516457896242926	37.0	35.0	37.0	33.0	37.0
6	35.549191709666914	37.0	35.0	37.0	33.0	37.0
7	35.73036900870203	37.0	35.0	37.0	35.0	37.0
8	35.784137228036904	37.0	35.0	37.0	35.0	37.0
9	37.460964831886955	39.0	37.0	39.0	35.0	39.0
10	36.9698376298414	39.0	37.0	39.0	33.0	39.0
11	36.97385963084624	39.0	37.0	39.0	33.0	39.0
12	36.946507386635616	39.0	37.0	39.0	33.0	39.0
13	36.92845058015716	39.0	37.0	39.0	33.0	39.0
14	38.10643533347663	40.0	37.0	41.0	33.0	41.0
15	38.10494257769385	40.0	37.0	41.0	33.0	41.0
16	38.013801397546445	40.0	37.0	41.0	33.0	41.0
17	37.973870180357075	40.0	37.0	41.0	33.0	41.0
18	37.94082779374124	40.0	37.0	41.0	33.0	41.0
19	38.008181145650504	40.0	37.0	41.0	33.0	41.0
20	37.92592529100166	40.0	37.0	41.0	33.0	41.0
21	37.90002228584164	40.0	37.0	41.0	33.0	41.0
22	37.8870068268522	40.0	37.0	41.0	33.0	41.0
23	37.75400123165539	40.0	37.0	41.0	33.0	41.0
24	37.63574517129109	39.0	36.0	41.0	32.0	41.0
25	37.659606846105056	39.0	37.0	41.0	33.0	41.0
26	37.62262421719333	39.0	37.0	41.0	33.0	41.0
27	37.60142365648683	40.0	37.0	41.0	33.0	41.0
28	37.50139978821857	39.0	36.0	41.0	32.0	41.0
29	37.361163822035024	39.0	36.0	41.0	32.0	41.0
30	37.38963299570503	39.0	36.0	41.0	32.0	41.0
31	37.3381447893988	39.0	36.0	41.0	32.0	41.0
32	37.26133775709488	39.0	36.0	41.0	32.0	41.0
33	37.22396743366006	39.0	36.0	41.0	31.0	41.0
34	37.175100780795674	39.0	36.0	41.0	31.0	41.0
35	37.08947435743589	39.0	36.0	41.0	31.0	41.0
36	37.04757038172086	39.0	35.0	41.0	31.0	41.0
37	36.996144153790766	39.0	35.0	41.0	31.0	41.0
38	36.88888727715807	39.0	35.0	41.0	31.0	41.0
39	36.79849511227976	39.0	35.0	40.0	31.0	41.0
40	36.71720190049437	39.0	35.0	40.0	31.0	41.0
41	36.598323418990965	39.0	35.0	40.0	30.0	41.0
42	36.52080297601701	39.0	35.0	40.0	30.0	41.0
43	36.43974185346993	39.0	35.0	40.0	30.0	41.0
44	36.365901871087615	39.0	35.0	40.0	30.0	41.0
45	36.16273807278899	38.0	35.0	40.0	30.0	41.0
46	35.998114274938715	38.0	35.0	40.0	30.0	41.0
47	35.86450735762446	38.0	35.0	40.0	29.0	41.0
48	35.80959319767542	38.0	35.0	40.0	29.0	41.0
49	35.64141025860807	38.0	34.0	40.0	28.0	41.0
50	35.546232571878434	38.0	34.0	40.0	28.0	41.0
51	34.253140787178125	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	4.0
11	5.0
12	11.0
13	11.0
14	15.0
15	47.0
16	60.0
17	158.0
18	262.0
19	455.0
20	809.0
21	1250.0
22	1731.0
23	2207.0
24	2908.0
25	3702.0
26	4504.0
27	5865.0
28	7768.0
29	9999.0
30	13024.0
31	17146.0
32	22566.0
33	30582.0
34	45032.0
35	57559.0
36	72663.0
37	107089.0
38	164706.0
39	186147.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.029828741878525	31.35868468698942	22.112697786844496	26.49878878428756
2	23.24175918367885	29.010759182360164	21.350231891434984	26.397249742526004
3	20.927592113713178	29.59441086916101	22.489710930216305	26.988286086909508
4	19.895718085422025	30.7072523930906	20.927592113713178	28.4694374077742
5	18.027531585894778	33.762654467915645	20.458534488328944	27.75127945786064
6	20.026927626399623	35.40112536906804	20.615062855304227	23.956884149228106
7	84.25973423144836	4.521652211639012	6.291728260425225	4.926885296487408
8	86.59328602756851	3.521294846959565	4.583630587779183	5.301788537692743
9	78.46831652224826	6.575114495159752	9.403833955974255	5.552735026617735
10	33.36797089389961	28.23220528293129	16.036311416285017	22.363512406884084
11	23.559431328618583	27.406969798069174	29.107946550903367	19.925652322408876
12	22.17493990075548	22.768613622847074	35.52666454797324	19.529781928424207
13	24.33983139244312	24.66343763722606	29.818324236578054	21.178406733752762
14	19.97286138338373	28.286535263717987	30.13480956154914	21.605793791349136
15	19.221736212119016	32.07315030811165	27.795983010012804	20.909130469756533
16	20.116466599589362	30.124523788487583	27.79611487889821	21.96289473302485
17	18.47891878063479	30.552042714969364	30.400789103410265	20.56824940098559
18	19.369956839313808	29.597575722410724	28.81994490517968	22.212522533095793
19	18.402434827100112	33.630521844740215	29.462410114870984	18.50463321328869
20	20.243060729577795	30.884352306189	29.209749330435734	19.662837633797466
21	19.05980122084214	32.248799663470606	28.4809100008044	20.210489114882854
22	18.20660953227425	30.71832937946459	29.529267639771128	21.545793448490038
23	18.873997961307033	30.75960434059623	27.555058556378565	22.81133914171817
24	16.624842251845834	31.410245421182626	28.56425113638012	23.400661190591418
25	17.988366526929607	31.646290726056897	27.79070825459662	22.574634492416877
26	20.29923687476016	30.611647451172246	28.242227318222042	20.84688835584555
27	17.702342914486984	29.36311284416131	28.107984792880135	24.826559448471574
28	18.636633967578717	31.401014599204302	29.026847186379527	20.935504246837453
29	20.436776122237184	31.854116089454575	26.842439099651997	20.866668688656244
30	19.758046969059603	31.503476723163693	26.83940611528769	21.899070192489013
31	20.907548043131673	31.346684618417598	26.145380171403175	21.600387167047547
32	20.3316766205697	31.934819847322203	25.609860627775017	22.123642904333078
33	20.537392081800906	29.181265651188337	28.353260919732726	21.928081347278027
34	19.064548500716707	31.25622256303003	28.028995330522765	21.650233605730495
35	20.62073321737663	31.037979557685386	27.39233235178926	20.948954873148725
36	21.74689349873208	29.94043482446273	28.22033708324487	20.092334593560317
37	20.37967689485698	30.883033617334952	27.046440265372944	21.69084922243512
38	20.58921655376492	31.686642604990713	28.688603495316677	19.035537345927693
39	21.543815415208968	30.251117918476016	27.81154353849055	20.393523127824466
40	23.260616434291713	29.999380216238595	26.23017186471835	20.50983148475134
41	20.088642264768986	29.61445493974251	29.232035172069114	21.064867623419385
42	21.412737743116775	30.614548566651152	27.05909967837179	20.913614011860286
43	19.087625555662516	28.19857871715311	29.75027989170927	22.963515835475103
44	21.282846890993223	26.971538738463117	27.818005113875376	23.92760925666828
45	22.05177436178756	27.116067036866585	29.979863621198717	20.85229498014714
46	19.978531745456127	28.23022724965022	30.39828359458757	21.39295741030608
47	20.436644253351776	28.9804293387171	28.36526098830455	22.217665419626574
48	21.018581644642364	27.662663566868734	30.75986807836704	20.55888671012186
49	20.571941729776917	27.081253651119763	29.59032293371347	22.75648168538985
50	19.802354914555558	26.94991224125676	27.866400994818875	25.38133184936881
51	20.539765721738192	25.69742156768368	30.863517022295074	22.899295688283054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1477.0
1	1173.0
2	869.0
3	654.0
4	439.0
5	477.5
6	516.0
7	565.5
8	615.0
9	677.0
10	739.0
11	905.0
12	1071.0
13	1206.0
14	1341.0
15	1566.0
16	1791.0
17	2145.5
18	2500.0
19	2959.5
20	3419.0
21	4226.0
22	5033.0
23	6345.0
24	7657.0
25	9511.5
26	12764.5
27	14163.0
28	17427.5
29	20692.0
30	22374.0
31	24056.0
32	26435.5
33	28815.0
34	30034.0
35	31253.0
36	33634.5
37	36016.0
38	36217.5
39	36419.0
40	36844.0
41	37269.0
42	39304.5
43	41340.0
44	45882.0
45	50424.0
46	58011.0
47	65598.0
48	70486.0
49	75374.0
50	69757.5
51	64141.0
52	56547.0
53	48953.0
54	41235.0
55	33517.0
56	28632.5
57	23748.0
58	22512.0
59	21276.0
60	19172.0
61	17068.0
62	15392.0
63	13716.0
64	11804.0
65	9892.0
66	8726.5
67	7561.0
68	6731.0
69	5901.0
70	4857.0
71	3813.0
72	3297.0
73	2781.0
74	2506.5
75	1932.0
76	1632.0
77	1371.0
78	1110.0
79	710.0
80	310.0
81	246.0
82	182.0
83	171.5
84	161.0
85	103.0
86	45.0
87	31.5
88	18.0
89	15.5
90	13.0
91	9.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	758329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.53883210741312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64335286360834	25.034508796269545
2	10.037697468217136	6.733053002630804
3	3.751577819844117	3.774706159129403
4	2.0145683425763092	2.7026507764230536
5	1.2986108819306899	2.1776946170966545
6	0.9656817906956076	1.9432703668395626
7	0.7481946067302068	1.7565501309157474
8	0.5980660998414221	1.6046750809373462
9	0.5149918982896674	1.5545004132073612
>10	4.7828778041651985	34.07541908488224
>50	0.563981391724326	12.64919312788482
>100	0.0752503258700737	4.021488872316662
>500	0.0031684347734767877	0.6834206522896524
>1k	0.0015842173867383939	0.6018618028230156
>5k	3.9605434668459847E-4	0.6870071163541461
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT	5172	0.6820258753126941	Illumina Single End Adapter 1 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT	1287	0.16971525551574582	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1136	0.1498030538196482	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1105	0.145715118372105	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC	1003	0.13226449206083377	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGT	982	0.12949524546733673	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7673450441694831	0.0
2	0.0	0.0	0.0	1.1262921502408585	0.0
3	0.0	0.0	0.0	1.5662067519506704	0.0
4	0.0	0.0	0.0	3.387711666044685	0.0
5	0.0	0.0	0.0	3.681647411611583	0.0
6	0.0	0.0	0.0	5.236777177188265	0.0
7	0.0	0.0	0.0	6.3477725367221876	0.0
8	0.0	0.0	0.0	7.44162494115351	0.0
9	0.0	0.0	0.0	8.832314206630631	0.0
10	0.0	0.0	0.0	11.1377779301596	0.0
11	0.0	0.0	0.0	13.134140986300142	0.0
12	0.0	0.0	0.0	14.080168370192885	0.0
13	0.0	0.0	0.0	14.473137648698652	0.0
14	0.0	0.0	0.0	14.845667249966704	0.0
15	0.0	0.0	0.0	15.18153730109227	0.0
16	0.0	0.0	0.0	15.893101806735599	0.0
17	0.0	0.0	0.0	16.880799758416202	0.0
18	0.0	0.0	0.0	18.012630401844053	0.0
19	0.0	0.0	0.0	18.5534247008884	0.0
20	0.0	0.0	0.0	19.09975749311974	0.0
21	0.0	0.0	0.0	19.682090491066543	0.0
22	0.0	0.0	0.0	20.307544614540653	0.0
23	0.0	0.0	0.0	20.973218748063175	0.0
24	0.0	0.0	0.0	21.493573369869807	0.0
25	0.0	0.0	0.0	21.954191386588143	0.0
26	0.0	0.0	0.0	22.358105782582495	0.0
27	0.0	0.0	0.0	22.81410838831167	0.0
28	0.0	0.0	0.0	23.241891052564256	0.0
29	0.0	0.0	0.0	23.689981525169156	0.0
30	0.0	0.0	0.0	24.337457752505838	0.0
31	0.0	0.0	0.0	24.76286677682114	0.0
32	0.0	0.0	0.0	25.20093521413529	0.0
33	0.0	0.0	0.0	25.61197052994149	0.0
34	0.0	0.0	0.0	26.00269803739538	0.0
35	0.0	0.0	0.0	26.429557619450133	0.0
36	0.0	0.0	0.0	26.825428013434802	0.0
37	0.0	0.0	0.0	27.215100569805454	0.0
38	0.0	0.0	0.0	27.62336663901816	0.0
39	0.0	0.0	0.0	28.02477552618982	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACGA	20	7.0317666E-4	45.0	9
CGACTAG	25	3.8895465E-5	45.0	1
CGATACG	35	1.2111923E-7	45.0	1
TGTAGCG	225	0.0	41.000004	1
CATTGCG	40	3.4561526E-7	39.375	1
CTAGGGA	650	0.0	39.115383	4
TAGGGAA	700	0.0	38.892857	5
TACACGC	465	0.0	38.70968	35
CACTAGG	170	0.0	38.382355	2
CACGGGC	505	0.0	38.31683	4
AGACGGG	330	0.0	38.181816	3
TAGGGAC	325	0.0	38.07692	5
ACGGGAG	705	0.0	37.97872	5
TCGCCGA	30	1.13955546E-4	37.500004	44
GCGACGG	150	0.0	37.500004	2
TAGCCGT	30	1.13955546E-4	37.500004	44
AACATAG	60	1.546141E-10	37.500004	1
CGCTACG	30	1.13955546E-4	37.500004	1
CGCTAAG	30	1.13955546E-4	37.500004	1
ATAGGGC	510	0.0	37.5	4
>>END_MODULE