##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553114_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	961414
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8405640026045	33.0	31.0	34.0	30.0	34.0
2	32.04995350598181	33.0	31.0	34.0	30.0	34.0
3	32.19993675981419	34.0	31.0	34.0	30.0	34.0
4	35.85143444967517	37.0	35.0	37.0	35.0	37.0
5	35.631689365871516	37.0	35.0	37.0	33.0	37.0
6	34.930757197211605	37.0	35.0	37.0	33.0	37.0
7	35.420005325489335	37.0	35.0	37.0	33.0	37.0
8	35.84635339198306	37.0	35.0	37.0	35.0	37.0
9	37.50024235136996	39.0	37.0	39.0	35.0	39.0
10	37.06417214644264	39.0	37.0	39.0	33.0	39.0
11	37.07460053629342	39.0	37.0	39.0	33.0	39.0
12	36.983046845583694	39.0	37.0	39.0	33.0	39.0
13	36.97979018404142	39.0	37.0	39.0	33.0	39.0
14	38.19167601054281	40.0	37.0	41.0	33.0	41.0
15	38.18514396503483	40.0	37.0	41.0	33.0	41.0
16	38.06890995970518	40.0	37.0	41.0	33.0	41.0
17	38.04779938715267	40.0	37.0	41.0	33.0	41.0
18	38.012350558656316	40.0	37.0	41.0	33.0	41.0
19	38.08307659343426	40.0	37.0	41.0	33.0	41.0
20	37.987270832336534	40.0	37.0	41.0	33.0	41.0
21	38.01193762520621	40.0	37.0	41.0	33.0	41.0
22	37.99252975305124	40.0	37.0	41.0	33.0	41.0
23	37.92176939383034	40.0	37.0	41.0	33.0	41.0
24	37.79453908513918	40.0	37.0	41.0	33.0	41.0
25	37.82771313918874	40.0	37.0	41.0	33.0	41.0
26	37.77922206250377	40.0	37.0	41.0	33.0	41.0
27	37.71579673272908	40.0	37.0	41.0	33.0	41.0
28	37.64341168320828	40.0	37.0	41.0	33.0	41.0
29	37.427649274922146	40.0	36.0	41.0	32.0	41.0
30	37.48666235357505	40.0	36.0	41.0	32.0	41.0
31	37.46473215493013	40.0	36.0	41.0	32.0	41.0
32	37.43787691878837	40.0	36.0	41.0	32.0	41.0
33	37.22394514745989	39.0	36.0	41.0	32.0	41.0
34	37.243798197238654	39.0	36.0	41.0	32.0	41.0
35	37.16647666874	39.0	36.0	41.0	31.0	41.0
36	37.17963125146919	39.0	36.0	41.0	32.0	41.0
37	37.11341836087263	39.0	35.0	41.0	31.0	41.0
38	36.97549130759486	39.0	35.0	41.0	31.0	41.0
39	36.95089108334183	39.0	35.0	41.0	31.0	41.0
40	36.88995375561413	39.0	35.0	41.0	31.0	41.0
41	36.86980218719511	39.0	35.0	41.0	31.0	41.0
42	36.80402199260672	39.0	35.0	41.0	31.0	41.0
43	36.74601368401126	39.0	35.0	40.0	31.0	41.0
44	36.679124705901934	39.0	35.0	40.0	31.0	41.0
45	36.51329812130882	39.0	35.0	40.0	31.0	41.0
46	36.402888869935325	38.0	35.0	40.0	30.0	41.0
47	36.283178734655415	38.0	35.0	40.0	30.0	41.0
48	36.19527279611073	38.0	35.0	40.0	30.0	41.0
49	36.09346649830354	38.0	35.0	40.0	30.0	41.0
50	36.0059776537475	38.0	35.0	40.0	30.0	41.0
51	34.86282496406335	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	6.0
11	5.0
12	7.0
13	19.0
14	20.0
15	48.0
16	61.0
17	131.0
18	268.0
19	519.0
20	948.0
21	1472.0
22	2112.0
23	2705.0
24	3369.0
25	4189.0
26	5159.0
27	6731.0
28	8438.0
29	11293.0
30	15017.0
31	20263.0
32	27203.0
33	37351.0
34	58172.0
35	72739.0
36	87901.0
37	130096.0
38	204742.0
39	260357.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.404231683749146	31.55466843628239	22.3859856419815	26.655114237986965
2	22.626464769599778	30.68438778715517	21.13709598570439	25.552051457540664
3	21.638648906714486	28.76523537206656	22.2864447574094	27.309670963809555
4	20.609851739209123	29.71539836116387	20.74798161874073	28.92676828088628
5	18.036142598297925	34.43095274252299	18.680297977770245	28.852606681408844
6	19.144094011528853	36.8784935522054	20.50895867961149	23.468453756654263
7	86.03192797275679	4.677381440253627	5.011680711951355	4.279009875038224
8	88.2648890072331	2.98965898145856	3.857443307461718	4.8880087038466264
9	80.505380616467	6.776581160665436	7.712910359116884	5.005127863750683
10	33.507209173155374	33.12402357361137	14.282088673557904	19.086678579675354
11	25.04196943252335	27.00054295027948	28.096012747890086	19.861474869307084
12	24.2779905430959	22.028803408313173	34.435425321453614	19.257780727137323
13	26.01532742398176	23.322210826969442	28.797999613069912	21.864462135978883
14	20.8079973871818	27.65374750107654	29.571027673822098	21.967227437919565
15	20.112563370202636	30.423834061080868	29.215509655569814	20.248092913146678
16	22.247959775913394	28.85364681604387	27.568456461004313	21.329936947038426
17	20.308316708514752	29.643421044419988	28.246000162261005	21.80226208480426
18	21.373518588246064	28.41554210777043	28.964837208528273	21.246102095455235
19	21.002710590858882	31.92193997591048	28.083635145733265	18.991714287497373
20	21.610357244641744	30.16224020037154	28.361663133676025	19.865739421310693
21	22.192520599866448	30.150278652068724	27.60049260776315	20.05670814030168
22	19.747060059454096	29.25586687940887	28.926456240495767	22.070616820641263
23	20.496581077454664	28.95568402374003	27.834938954498273	22.712795944307032
24	19.526135462974327	29.372986039312927	28.89275587832089	22.208122619391855
25	20.58676075031152	29.241929075299506	27.352836551163186	22.818473623225792
26	20.411394050845942	29.720182980484992	27.431470729571235	22.436952239097828
27	19.04788155778884	28.393283226580852	27.895786830647356	24.663048384982954
28	20.635647078157795	29.40980680539289	28.665798500957962	21.28874761549135
29	21.408467111982976	30.712783462691412	26.060053213287926	21.818696212037686
30	22.208434659782363	28.82545916743463	27.56107150509562	21.405034667687385
31	21.837106595077664	30.334486495932033	25.38760617174287	22.44080073724743
32	22.486046593871112	28.73496745418727	26.35482736885462	22.424158583086996
33	21.560742822550953	29.471070735395987	28.196697780560715	20.77148866149234
34	20.96588982477892	28.005521034642722	29.34396628299567	21.684622857582685
35	20.30488426421916	30.126667595853608	28.68868146292856	20.87976667699867
36	22.168389476333815	31.09347273911135	27.353252605017193	19.38488517953764
37	22.98967978415126	31.189685192851364	25.287337192926252	20.533297830071124
38	22.813376963514155	30.417385226343697	26.146176361068175	20.62306144907397
39	21.600892019463	29.319003051755022	26.398200983135254	22.681903945646724
40	21.876943751599207	29.737033161572434	26.53841113193692	21.84761195489144
41	20.171330977081674	29.382035210637664	28.893796012955917	21.552837799324745
42	20.86832519601337	30.195732535619413	27.031122908549282	21.904819359817935
43	20.15500086331175	28.34439689873457	28.793319007212293	22.70728323074139
44	21.713226560045932	26.60924430058227	27.822561352341445	23.854967787030354
45	22.00800071561263	27.533923991121412	28.1731907378091	22.28488455545686
46	19.38956578539526	28.05940000873713	29.861537277385185	22.689496928482423
47	20.14085503227538	28.975758622196057	27.978789574522526	22.904596771006037
48	20.997197877293235	27.30863082917453	29.84603927132328	21.848132022208954
49	21.44570393191695	27.015208848633364	28.611711499936554	22.927375719513137
50	20.297915362164478	26.497845881170857	27.98513439579619	25.21910436086847
51	20.228746408935173	25.658561244167444	30.443908659536888	23.66878368736049
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1072.0
1	894.5
2	717.0
3	557.5
4	398.0
5	428.0
6	458.0
7	486.0
8	514.0
9	605.5
10	697.0
11	858.0
12	1019.0
13	1068.0
14	1117.0
15	1309.5
16	1502.0
17	1874.0
18	2246.0
19	3022.5
20	3799.0
21	4435.5
22	5072.0
23	6837.0
24	8602.0
25	9699.0
26	13295.5
27	15795.0
28	18028.0
29	20261.0
30	23662.0
31	27063.0
32	29930.5
33	32798.0
34	36540.5
35	40283.0
36	42731.0
37	45179.0
38	45884.5
39	46590.0
40	48043.5
41	49497.0
42	51852.5
43	54208.0
44	58842.5
45	63477.0
46	69451.5
47	75426.0
48	80766.0
49	86106.0
50	81212.5
51	76319.0
52	69217.0
53	62115.0
54	54696.5
55	47278.0
56	42470.5
57	37663.0
58	35842.5
59	34022.0
60	32190.0
61	30358.0
62	27784.5
63	25211.0
64	21622.0
65	18033.0
66	14348.0
67	10663.0
68	9552.5
69	8442.0
70	7113.5
71	5785.0
72	5082.5
73	4380.0
74	3582.0
75	2083.0
76	1382.0
77	1178.5
78	975.0
79	780.0
80	585.0
81	447.5
82	310.0
83	294.0
84	278.0
85	161.0
86	44.0
87	35.5
88	27.0
89	27.0
90	27.0
91	18.5
92	10.0
93	9.5
94	9.0
95	14.5
96	20.0
97	10.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	961414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.890457128144888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.44634556342761	22.252353004106922
2	9.586969514685592	5.731178025350834
3	3.6831409407715516	3.302722991611419
4	2.015190948321264	2.409399145832895
5	1.2993743927873085	1.9419447290509169
6	0.9389701481431191	1.6839748174607851
7	0.7195179309106271	1.5054703906770932
8	0.613883070948006	1.4679396491092238
9	0.49788141494815513	1.339371277956715
>10	5.208088447887933	33.66803789503802
>50	0.8651639784157659	17.693588650144278
>100	0.12085280616445744	5.268393598001874
>500	0.002843595439163531	0.5240026060327126
>1k	0.0017772471494772071	1.211623219626329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	4153	0.43196791392677863	Illumina Single End Adapter 1 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	2174	0.22612526965490412	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT	2008	0.2088590347134533	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	1970	0.20490652310035012	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1099	0.11431079638948465	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0401346350271579E-4	0.0	0.0	0.4767977166964492	0.0
2	1.0401346350271579E-4	0.0	0.0	0.7228935713438748	0.0
3	1.0401346350271579E-4	0.0	0.0	1.0059142055347645	0.0
4	1.0401346350271579E-4	0.0	0.0	2.1995727126919307	0.0
5	1.0401346350271579E-4	0.0	0.0	2.40406318193827	0.0
6	1.0401346350271579E-4	0.0	0.0	3.5905447601137492	0.0
7	1.0401346350271579E-4	0.0	0.0	4.468730432467178	0.0
8	1.0401346350271579E-4	0.0	0.0	5.373335524550297	0.0
9	1.0401346350271579E-4	0.0	0.0	6.531941494507049	0.0
10	1.0401346350271579E-4	0.0	0.0	8.154239484758907	0.0
11	1.0401346350271579E-4	0.0	0.0	9.713297289201114	0.0
12	1.0401346350271579E-4	0.0	0.0	10.441495547183628	0.0
13	1.0401346350271579E-4	0.0	0.0	10.768097822582154	0.0
14	1.0401346350271579E-4	0.0	0.0	11.10000478461932	0.0
15	1.0401346350271579E-4	0.0	0.0	11.357438106788543	0.0
16	1.0401346350271579E-4	0.0	0.0	11.84297295441922	0.0
17	1.0401346350271579E-4	0.0	0.0	12.591973905102277	0.0
18	1.0401346350271579E-4	0.0	0.0	13.422209370780953	0.0
19	1.0401346350271579E-4	0.0	0.0	13.813299993551166	0.0
20	1.0401346350271579E-4	0.0	0.0	14.257853536561772	0.0
21	1.0401346350271579E-4	0.0	0.0	14.70355122767091	0.0
22	1.0401346350271579E-4	0.0	0.0	15.217273723910823	0.0
23	1.0401346350271579E-4	0.0	0.0	15.718722631457416	0.0
24	1.0401346350271579E-4	0.0	0.0	16.10991726769113	0.0
25	2.0802692700543158E-4	0.0	0.0	16.452433603005574	0.0
26	2.0802692700543158E-4	0.0	0.0	16.755840876042996	0.0
27	2.0802692700543158E-4	0.0	0.0	17.136218112072427	0.0
28	2.0802692700543158E-4	0.0	0.0	17.49714483042685	0.0
29	2.0802692700543158E-4	0.0	0.0	17.84871033706603	0.0
30	2.0802692700543158E-4	0.0	0.0	18.404350259097537	0.0
31	2.0802692700543158E-4	0.0	0.0	18.753107402222145	0.0
32	2.0802692700543158E-4	0.0	0.0	19.087510687383375	0.0
33	2.0802692700543158E-4	0.0	0.0	19.437932045924025	0.0
34	2.0802692700543158E-4	0.0	0.0	19.76016575585544	0.0
35	2.0802692700543158E-4	0.0	0.0	20.089056327451026	0.0
36	2.0802692700543158E-4	0.0	0.0	20.4250198145648	0.0
37	2.0802692700543158E-4	0.0	0.0	20.755158547722417	0.0
38	2.0802692700543158E-4	0.0	0.0	21.122326073887002	0.0
39	2.0802692700543158E-4	0.0	0.0	21.486997276927525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCG	35	1.211647E-7	45.000004	9
CTATACG	35	1.211647E-7	45.000004	1
CGCGACG	60	0.0	45.000004	1
ACCGCAC	20	7.03271E-4	45.0	10
CCGTACG	20	7.03271E-4	45.0	1
ACGGAGT	20	7.03271E-4	45.0	32
TAGTGCG	20	7.03271E-4	45.0	1
TCGCACG	45	1.927765E-8	40.0	1
CGGGAAT	1565	0.0	39.824284	6
CCTCGCG	40	3.457426E-7	39.375	1
CTAACCG	160	0.0	39.375	1
AGGGCGT	200	0.0	39.375	6
TTACGGG	460	0.0	38.641308	3
AGCACGG	1240	0.0	38.46774	2
ATGCGGG	395	0.0	38.16456	3
ACTGCGG	130	0.0	38.07692	2
CGTACAG	65	9.094947E-12	38.07692	1
TAGACGG	155	0.0	37.741936	2
ACGGGAA	1705	0.0	37.741936	5
GCACGGG	1525	0.0	37.62295	3
>>END_MODULE