Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553113_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 983189 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT | 4377 | 0.4451839880226487 | Illumina Single End Adapter 1 (95% over 22bp) |
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC | 2399 | 0.24400191621346454 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT | 2279 | 0.23179673491058178 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT | 2190 | 0.2227445587776104 | No Hit |
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC | 1204 | 0.12245865240559038 | Illumina Single End Adapter 1 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGACGGT | 20 | 7.03279E-4 | 45.0 | 28 |
CGCATTG | 20 | 7.03279E-4 | 45.0 | 1 |
CTATACG | 20 | 7.03279E-4 | 45.0 | 1 |
GACTACG | 50 | 1.0804797E-9 | 40.5 | 1 |
AGTACGG | 165 | 0.0 | 39.545452 | 2 |
CCTCGCG | 40 | 3.457535E-7 | 39.375 | 1 |
CTCAACG | 75 | 0.0 | 39.0 | 1 |
ACCGGGT | 145 | 0.0 | 38.793106 | 4 |
CATAGCG | 70 | 0.0 | 38.571426 | 1 |
TACTCGG | 35 | 6.247368E-6 | 38.571426 | 2 |
TCGCGCG | 35 | 6.247368E-6 | 38.571426 | 1 |
AGCACGG | 1130 | 0.0 | 38.23009 | 2 |
CGGGAAT | 1455 | 0.0 | 38.195877 | 6 |
ACGGGAA | 1625 | 0.0 | 38.076923 | 5 |
TTGGACG | 30 | 1.1398017E-4 | 37.500004 | 15 |
TGCGCGT | 30 | 1.1398017E-4 | 37.500004 | 32 |
ACGTCCG | 30 | 1.1398017E-4 | 37.500004 | 19 |
CACGGGA | 1820 | 0.0 | 37.335163 | 4 |
ACTACGG | 175 | 0.0 | 37.285713 | 2 |
ATTGCGG | 145 | 0.0 | 37.241383 | 2 |