FastQCFastQC Report
Sat 18 Jun 2016
SRR3553113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983189
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT43770.4451839880226487Illumina Single End Adapter 1 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC23990.24400191621346454No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT22790.23179673491058178No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT21900.2227445587776104No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC12040.12245865240559038Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT207.03279E-445.028
CGCATTG207.03279E-445.01
CTATACG207.03279E-445.01
GACTACG501.0804797E-940.51
AGTACGG1650.039.5454522
CCTCGCG403.457535E-739.3751
CTCAACG750.039.01
ACCGGGT1450.038.7931064
CATAGCG700.038.5714261
TACTCGG356.247368E-638.5714262
TCGCGCG356.247368E-638.5714261
AGCACGG11300.038.230092
CGGGAAT14550.038.1958776
ACGGGAA16250.038.0769235
TTGGACG301.1398017E-437.50000415
TGCGCGT301.1398017E-437.50000432
ACGTCCG301.1398017E-437.50000419
CACGGGA18200.037.3351634
ACTACGG1750.037.2857132
ATTGCGG1450.037.2413832