##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553113_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	983189
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.845200668437094	33.0	31.0	34.0	30.0	34.0
2	32.05459581016468	33.0	31.0	34.0	30.0	34.0
3	32.18669452160266	34.0	31.0	34.0	30.0	34.0
4	35.84203037259367	37.0	35.0	37.0	35.0	37.0
5	35.63283661635759	37.0	35.0	37.0	33.0	37.0
6	35.655487398658856	37.0	35.0	37.0	33.0	37.0
7	35.80215909657248	37.0	35.0	37.0	35.0	37.0
8	35.85724107979239	37.0	35.0	37.0	35.0	37.0
9	37.531103378902735	39.0	37.0	39.0	35.0	39.0
10	37.08742571367255	39.0	37.0	39.0	33.0	39.0
11	37.0859804167866	39.0	37.0	39.0	33.0	39.0
12	37.01728558802021	39.0	37.0	39.0	33.0	39.0
13	37.014757081293624	39.0	37.0	39.0	33.0	39.0
14	38.20814919613625	40.0	37.0	41.0	33.0	41.0
15	38.20423031583958	40.0	37.0	41.0	33.0	41.0
16	38.10639154831879	40.0	37.0	41.0	33.0	41.0
17	38.05678867440543	40.0	37.0	41.0	33.0	41.0
18	37.990801361691396	40.0	37.0	41.0	33.0	41.0
19	38.071252831347785	40.0	37.0	41.0	33.0	41.0
20	37.98931029537556	40.0	37.0	41.0	33.0	41.0
21	37.99790884560344	40.0	37.0	41.0	33.0	41.0
22	37.96899273690003	40.0	37.0	41.0	33.0	41.0
23	37.86982665591255	40.0	37.0	41.0	33.0	41.0
24	37.76408605059658	40.0	37.0	41.0	33.0	41.0
25	37.8029046297304	40.0	37.0	41.0	33.0	41.0
26	37.73417827091231	40.0	37.0	41.0	33.0	41.0
27	37.69902938295689	40.0	37.0	41.0	33.0	41.0
28	37.617211950093015	40.0	36.0	41.0	33.0	41.0
29	37.45814894186164	40.0	36.0	41.0	32.0	41.0
30	37.494220338103865	40.0	36.0	41.0	32.0	41.0
31	37.45407749679868	40.0	36.0	41.0	32.0	41.0
32	37.40169794413892	40.0	36.0	41.0	32.0	41.0
33	37.33829202727044	39.0	36.0	41.0	32.0	41.0
34	37.307328499403475	39.0	36.0	41.0	32.0	41.0
35	37.20508061013701	39.0	36.0	41.0	31.0	41.0
36	37.181126924731664	39.0	36.0	41.0	32.0	41.0
37	37.11467174673435	39.0	35.0	41.0	31.0	41.0
38	37.076065741174894	39.0	35.0	41.0	31.0	41.0
39	36.99927786010625	39.0	35.0	41.0	31.0	41.0
40	36.91673625315173	39.0	35.0	41.0	31.0	41.0
41	36.84866592282867	39.0	35.0	41.0	31.0	41.0
42	36.77419092361692	39.0	35.0	41.0	31.0	41.0
43	36.70555813785549	39.0	35.0	40.0	31.0	41.0
44	36.66837708721314	39.0	35.0	40.0	31.0	41.0
45	36.51663718776349	39.0	35.0	40.0	31.0	41.0
46	36.40244856278905	38.0	35.0	40.0	30.0	41.0
47	36.26485751976477	38.0	35.0	40.0	30.0	41.0
48	36.19156133764719	38.0	35.0	40.0	30.0	41.0
49	36.026138412858565	38.0	35.0	40.0	30.0	41.0
50	35.95546532762266	38.0	35.0	40.0	29.0	41.0
51	34.73375007246826	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	4.0
12	10.0
13	18.0
14	22.0
15	35.0
16	76.0
17	119.0
18	255.0
19	505.0
20	919.0
21	1419.0
22	1961.0
23	2701.0
24	3345.0
25	4275.0
26	5393.0
27	6877.0
28	8925.0
29	11723.0
30	15206.0
31	20499.0
32	27541.0
33	37749.0
34	59341.0
35	73318.0
36	88399.0
37	131055.0
38	212838.0
39	268586.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.43268283107317	31.67183522191562	22.334464685833545	26.561017261177653
2	22.543478415645417	30.759599629369326	21.199687954198023	25.497234000787234
3	21.635718056243512	28.80483813386846	22.26540370162807	27.29404010825996
4	20.58281774918149	29.652284555665286	20.75684329259176	29.008054402561463
5	17.941107966016705	34.52489806130866	18.688370191285706	28.84562378138893
6	19.539478167473394	35.539657176799174	20.954872359231032	23.965992296496403
7	85.83151357470436	4.653327081568244	5.13797448913688	4.377184854590521
8	87.843232582952	3.061873149516522	3.9886532497820864	5.106241017749385
9	80.29280229945616	6.746108835635875	7.908652354735459	5.052436510172511
10	33.50596884220633	32.860416461128025	14.433745698944964	19.19986899772068
11	25.07778260334483	26.867570731568396	28.16467637453226	19.889970290554512
12	24.177853901945607	21.838324065871365	34.660477283614846	19.32334474856818
13	25.85240477670112	23.241207946793548	29.071826474869024	21.834560801636307
14	20.635300028783888	27.56682591037939	29.697850565862716	22.100023494974007
15	20.0801676991911	30.452029060536685	29.29263854660701	20.175164693665206
16	22.059848106518686	28.82660404052527	27.7250864279401	21.388461425015944
17	20.109663554006403	29.727143000989635	28.412644974669167	21.7505484703348
18	21.22918380901332	28.620438186350743	28.958216578908026	21.192161425727914
19	20.835871841527926	32.00229050569117	28.138130105198496	19.023707547582408
20	21.47206691694069	30.313195123216392	28.48150253918626	19.73323542065666
21	22.03960784752474	30.188193724706032	27.614934666681584	20.157263761087645
22	19.602233141339052	29.371667095543174	28.889155594702544	22.13694416841523
23	20.461477905061997	28.953639635919444	27.883957204565956	22.700925254452603
24	19.445701691129578	29.36983631834774	28.9115317604245	22.272930230098183
25	20.556983448757055	29.175672225787718	27.411616688144395	22.85572763731083
26	20.311252465192347	29.71798911501247	27.55370534047879	22.41705307931639
27	19.0445580656415	28.397083368507985	28.002245753359727	24.55611281249078
28	20.636622256758365	29.16255165588712	28.908175335566206	21.292650751788315
29	21.457624119065613	30.66083937066017	26.02724399886492	21.8542925114093
30	22.211090644830243	28.783275646900037	27.548823267957633	21.456810440312086
31	21.81879577578675	30.38398517477311	25.31649560766038	22.48072344177976
32	22.53208691309606	28.722046320697242	26.283451096381267	22.462415669825436
33	21.51478505150078	29.434523779253023	28.36646870540659	20.684222463839607
34	20.976129716666886	27.896569225245603	29.408180929607635	21.719120128479872
35	20.320914900390463	30.117505382993503	28.62460828996256	20.93697142665347
36	22.22583857223789	31.000651960101262	27.3930037866575	19.380505681003346
37	23.067487532915848	31.087308747351727	25.29015275801499	20.55505096171743
38	22.846268621801098	30.479490718468167	26.10759477577556	20.56664588395517
39	21.613138470833178	29.280229945615748	26.344985552116633	22.76164603143444
40	22.027911215442806	29.73070284553631	26.418725189154884	21.822660749865996
41	20.243411999117157	29.18116455737401	28.948147304333144	21.627276139175684
42	20.93249619350908	30.192058698785278	27.040375756848377	21.83506935085726
43	20.108849875252876	28.355178912701422	28.766086683231812	22.76988452881389
44	21.76071945475387	26.49083746868608	27.867581919651258	23.880861156908793
45	22.14172453109219	27.411006429079247	28.24980751411987	22.19746152570869
46	19.34704314226461	27.98444653062636	29.942259321452948	22.726251005656085
47	20.214526403367003	28.936043832874454	27.90480772262505	22.944622041133496
48	21.154121944000597	27.150324098418515	29.83078533221995	21.86476862536094
49	21.413888886063614	26.870622026894114	28.755813988968548	22.959675098073717
50	20.234461532828377	26.337967572867477	28.033572385370466	25.393998508933684
51	20.210152880066804	25.475468094130427	30.57825097717733	23.736128048625442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1099.0
1	915.0
2	731.0
3	578.5
4	426.0
5	480.5
6	535.0
7	542.0
8	549.0
9	647.5
10	746.0
11	887.5
12	1029.0
13	1105.0
14	1181.0
15	1379.5
16	1578.0
17	1958.5
18	2339.0
19	3061.0
20	3783.0
21	4511.5
22	5240.0
23	7044.5
24	8849.0
25	9862.0
26	13481.5
27	16088.0
28	18548.0
29	21008.0
30	24334.0
31	27660.0
32	30423.5
33	33187.0
34	36859.0
35	40531.0
36	43308.5
37	46086.0
38	46356.0
39	46626.0
40	48497.0
41	50368.0
42	52723.5
43	55079.0
44	60386.0
45	65693.0
46	71928.0
47	78163.0
48	83747.0
49	89331.0
50	84246.0
51	79161.0
52	71576.5
53	63992.0
54	56084.0
55	48176.0
56	43141.5
57	38107.0
58	35936.0
59	33765.0
60	32445.5
61	31126.0
62	28216.5
63	25307.0
64	21741.0
65	18175.0
66	14455.5
67	10736.0
68	9751.5
69	8767.0
70	7398.0
71	6029.0
72	5270.0
73	4511.0
74	3674.0
75	2110.0
76	1383.0
77	1169.5
78	956.0
79	788.0
80	620.0
81	490.5
82	361.0
83	312.5
84	264.0
85	153.5
86	43.0
87	32.5
88	22.0
89	24.0
90	26.0
91	19.0
92	12.0
93	14.5
94	17.0
95	15.5
96	14.0
97	8.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	983189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.90555024091425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.61796664690026	22.31491350433739
2	9.665937102265254	5.781303352746212
3	3.600245101993486	3.2300193233181487
4	1.9460343515220069	2.327889122799454
5	1.2790782102370308	1.91257688391511
6	0.9232885111359649	1.6566870573981307
7	0.7421987043957634	1.5537102450134277
8	0.6090414069591032	1.4570974715690048
9	0.50692440232002	1.3643867863734105
>10	5.129058595222055	33.45633314454507
>50	0.8444557789779608	17.409307929145967
>100	0.1306283400380679	5.60251294692153
>500	0.0034285653553290008	0.6568272531244446
>1k	0.0017142826776645004	1.2764349787927274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	4377	0.4451839880226487	Illumina Single End Adapter 1 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	2399	0.24400191621346454	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT	2279	0.23179673491058178	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	2190	0.2227445587776104	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1204	0.12245865240559038	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0170984419068968E-4	0.0	0.0	0.4984799463785701	0.0
2	1.0170984419068968E-4	0.0	0.0	0.7682144531722792	0.0
3	1.0170984419068968E-4	0.0	0.0	1.064596939143949	0.0
4	1.0170984419068968E-4	0.0	0.0	2.339326416385863	0.0
5	1.0170984419068968E-4	0.0	0.0	2.555866674667841	0.0
6	1.0170984419068968E-4	0.0	0.0	3.805880659771417	0.0
7	1.0170984419068968E-4	0.0	0.0	4.751883920589022	0.0
8	1.0170984419068968E-4	0.0	0.0	5.697276922341483	0.0
9	1.0170984419068968E-4	0.0	0.0	6.925219871255679	0.0
10	1.0170984419068968E-4	0.0	0.0	8.616552870302659	0.0
11	1.0170984419068968E-4	0.0	0.0	10.235773589818438	0.0
12	1.0170984419068968E-4	0.0	0.0	11.006327369407103	0.0
13	1.0170984419068968E-4	0.0	0.0	11.349903223083253	0.0
14	1.0170984419068968E-4	0.0	0.0	11.700598765852751	0.0
15	1.0170984419068968E-4	0.0	0.0	11.961891355578633	0.0
16	1.0170984419068968E-4	0.0	0.0	12.462507208685206	0.0
17	1.0170984419068968E-4	0.0	0.0	13.23570544422283	0.0
18	1.0170984419068968E-4	0.0	0.0	14.119869119772495	0.0
19	1.0170984419068968E-4	0.0	0.0	14.530268341081928	0.0
20	1.0170984419068968E-4	0.0	0.0	15.001896888594157	0.0
21	1.0170984419068968E-4	0.0	0.0	15.46101512527093	0.0
22	1.0170984419068968E-4	0.0	0.0	15.995195226960432	0.0
23	1.0170984419068968E-4	0.0	0.0	16.521747090335634	0.0
24	1.0170984419068968E-4	0.0	0.0	16.909871855767303	0.0
25	1.0170984419068968E-4	0.0	0.0	17.25883833118556	0.0
26	1.0170984419068968E-4	0.0	0.0	17.568748226434593	0.0
27	1.0170984419068968E-4	0.0	0.0	17.984029520265178	0.0
28	1.0170984419068968E-4	0.0	0.0	18.357101228756626	0.0
29	1.0170984419068968E-4	0.0	0.0	18.703118118693354	0.0
30	1.0170984419068968E-4	0.0	0.0	19.26130174361186	0.0
31	1.0170984419068968E-4	0.0	0.0	19.620032364072422	0.0
32	1.0170984419068968E-4	0.0	0.0	19.958014176318084	0.0
33	1.0170984419068968E-4	0.0	0.0	20.305251584385097	0.0
34	1.0170984419068968E-4	0.0	0.0	20.624620495143862	0.0
35	1.0170984419068968E-4	0.0	0.0	20.96046640066152	0.0
36	1.0170984419068968E-4	0.0	0.0	21.311161943431017	0.0
37	1.0170984419068968E-4	0.0	0.0	21.630835983722356	0.0
38	1.0170984419068968E-4	0.0	0.0	22.008179505669816	0.0
39	1.0170984419068968E-4	0.0	0.0	22.364774219402374	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.03279E-4	45.0	28
CGCATTG	20	7.03279E-4	45.0	1
CTATACG	20	7.03279E-4	45.0	1
GACTACG	50	1.0804797E-9	40.5	1
AGTACGG	165	0.0	39.545452	2
CCTCGCG	40	3.457535E-7	39.375	1
CTCAACG	75	0.0	39.0	1
ACCGGGT	145	0.0	38.793106	4
CATAGCG	70	0.0	38.571426	1
TACTCGG	35	6.247368E-6	38.571426	2
TCGCGCG	35	6.247368E-6	38.571426	1
AGCACGG	1130	0.0	38.23009	2
CGGGAAT	1455	0.0	38.195877	6
ACGGGAA	1625	0.0	38.076923	5
TTGGACG	30	1.1398017E-4	37.500004	15
TGCGCGT	30	1.1398017E-4	37.500004	32
ACGTCCG	30	1.1398017E-4	37.500004	19
CACGGGA	1820	0.0	37.335163	4
ACTACGG	175	0.0	37.285713	2
ATTGCGG	145	0.0	37.241383	2
>>END_MODULE