##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553112_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925765
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539957764659498	31.0	31.0	34.0	30.0	34.0
2	31.753166300302993	33.0	31.0	34.0	30.0	34.0
3	31.94868784194693	33.0	31.0	34.0	30.0	34.0
4	35.655039885932176	37.0	35.0	37.0	33.0	37.0
5	35.395389758740066	37.0	35.0	37.0	33.0	37.0
6	34.752463098086444	37.0	35.0	37.0	32.0	37.0
7	35.311312266071845	37.0	35.0	37.0	33.0	37.0
8	35.73483875497561	37.0	35.0	37.0	35.0	37.0
9	37.37630608199705	39.0	37.0	39.0	35.0	39.0
10	36.81345589863518	39.0	37.0	39.0	32.0	39.0
11	36.822362046523686	39.0	37.0	39.0	32.0	39.0
12	36.80529291990948	39.0	37.0	39.0	33.0	39.0
13	36.79768407749267	39.0	37.0	39.0	33.0	39.0
14	37.9489935350764	40.0	37.0	41.0	33.0	41.0
15	37.91527871544074	40.0	37.0	41.0	33.0	41.0
16	37.82437011552608	40.0	37.0	41.0	33.0	41.0
17	37.82916614907671	39.0	37.0	41.0	33.0	41.0
18	37.818142833224414	39.0	37.0	41.0	33.0	41.0
19	37.849108575070346	40.0	37.0	41.0	33.0	41.0
20	37.75433938418497	39.0	37.0	41.0	33.0	41.0
21	37.75732502308901	39.0	37.0	41.0	33.0	41.0
22	37.7279730817216	39.0	37.0	41.0	33.0	41.0
23	37.6407997710002	39.0	37.0	41.0	32.0	41.0
24	37.48650899526338	39.0	36.0	41.0	32.0	41.0
25	37.54074630170724	39.0	36.0	41.0	32.0	41.0
26	37.472266719955925	39.0	36.0	41.0	32.0	41.0
27	37.425806765215796	39.0	36.0	41.0	32.0	41.0
28	37.38604613481823	39.0	36.0	41.0	32.0	41.0
29	37.173770881379184	39.0	36.0	41.0	31.0	41.0
30	37.207509465145044	39.0	36.0	41.0	31.0	41.0
31	37.1789644240169	39.0	36.0	41.0	31.0	41.0
32	37.20407014739162	39.0	36.0	41.0	31.0	41.0
33	36.963284418832	39.0	36.0	41.0	31.0	41.0
34	37.03852813619007	39.0	36.0	41.0	31.0	41.0
35	36.981571997213116	39.0	35.0	40.0	31.0	41.0
36	36.954107143821595	39.0	35.0	40.0	31.0	41.0
37	36.84914962220434	39.0	35.0	40.0	31.0	41.0
38	36.68830210690618	39.0	35.0	40.0	30.0	41.0
39	36.62645055710683	39.0	35.0	40.0	30.0	41.0
40	36.56808801369678	39.0	35.0	40.0	30.0	41.0
41	36.61570160893963	39.0	35.0	40.0	30.0	41.0
42	36.55550922750374	39.0	35.0	40.0	30.0	41.0
43	36.476975258299895	39.0	35.0	40.0	30.0	41.0
44	36.36737292941513	39.0	35.0	40.0	30.0	41.0
45	36.11821358552116	38.0	35.0	40.0	29.0	41.0
46	35.97369202767441	38.0	35.0	40.0	29.0	41.0
47	35.880175854563525	38.0	35.0	40.0	28.0	41.0
48	35.80482411843178	38.0	34.0	40.0	28.0	41.0
49	35.70814731600352	38.0	34.0	40.0	28.0	41.0
50	35.621910528049774	38.0	34.0	40.0	28.0	41.0
51	34.34509405734717	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	3.0
11	9.0
12	7.0
13	7.0
14	19.0
15	32.0
16	58.0
17	160.0
18	241.0
19	451.0
20	847.0
21	1337.0
22	2008.0
23	2752.0
24	3650.0
25	4523.0
26	5948.0
27	7602.0
28	10059.0
29	13226.0
30	17662.0
31	22985.0
32	29980.0
33	40087.0
34	57266.0
35	72645.0
36	92609.0
37	133227.0
38	197219.0
39	209089.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.692540763584713	31.49881449395905	23.083935988074728	26.72470875438151
2	21.208514039740106	29.64175573714712	22.22367447462369	26.926055748489087
3	20.60641739534331	29.276544263392978	22.327372497340036	27.78966584392367
4	19.571057449784774	30.251521714473977	21.546180726210213	28.631240109531035
5	17.324482995144557	33.15895502638359	20.813489384455018	28.703072594016838
6	20.07777351703726	36.124718476071145	20.83779360852916	22.959714398362436
7	83.89407679054621	4.282296263090525	6.484042926660654	5.339584019702624
8	86.70094462417568	2.820154142790017	4.657121407700658	5.821779825333643
9	77.66171760652001	6.893002003748252	9.845803200596263	5.599477189135472
10	31.86640238073377	31.678125658239402	15.341150291920735	21.11432166910609
11	21.459009575864286	25.122898359734926	33.303916220639145	20.114175843761643
12	21.2504253239213	21.090449520126597	37.79231230387841	19.86681285207369
13	23.66550906547558	23.69607837842217	31.722737411762164	20.91567514434009
14	19.714398362435393	26.790816243863187	32.11030877166451	21.38447662203691
15	17.99668382364855	31.82254675862665	29.79978720301588	20.38098221470892
16	19.18294599601411	30.057952072070126	28.32133424789228	22.437767684023484
17	17.800737768224117	30.01139598062143	30.99803945925802	21.189826791896433
18	18.63691109514834	28.99094262582837	30.52416109919904	21.847985179824253
19	18.102434203064494	32.62501822816806	30.75953400701042	18.513013561757035
20	18.99996219342922	30.706280751594626	30.282739140062542	20.011017914913612
21	18.891187288350718	31.67466905748219	28.779441866996486	20.65470178717061
22	17.23936420149822	29.657526478101893	29.907049845263106	23.196059475136778
23	18.259709537517622	29.77235043450552	27.99220104454154	23.97573898343532
24	16.05493834828493	31.380209880477228	29.11008733317851	23.454764438059332
25	19.284267605709875	29.892035235723966	28.309020107694714	22.51467705087144
26	19.32034587611327	30.00232240363375	28.65241178916896	22.02491993108402
27	16.939504085810114	28.461434597333017	29.167877377088136	25.43118393976873
28	20.550355651812286	28.06597786695328	30.052335095839656	21.33133138539478
29	20.25287194914476	29.176302841433845	26.722008285039937	23.84881692438146
30	20.617651347804248	28.699075899391314	27.507844863437263	23.17542788936717
31	19.77391670672363	29.348916841747098	27.461504809535896	23.41566164199338
32	21.732405092004992	27.34182000831745	26.88619682100749	24.039578078670072
33	18.929209896680042	26.80388651547639	30.935172532986233	23.331731054857336
34	19.562523966665406	25.17690774656635	31.329657094403007	23.930911192365233
35	19.712994118377775	24.473273454926467	32.02637818452847	23.78735424216729
36	18.622112523156524	26.17748564700545	32.2688803314016	22.931521498436428
37	18.199327043040082	27.298396461304975	31.666675668231136	22.835600827423807
38	20.68753949436412	26.84115299239008	33.214476676046296	19.256830837199505
39	19.654123886731515	26.126716823383905	33.20853564349484	21.010623646389742
40	20.121953195465373	26.633756946957384	32.55124140575632	20.693048451820925
41	18.046696515854453	24.760387355322354	34.8357304499522	22.357185678870987
42	20.89579968998612	25.76172138717709	30.982484755850564	22.359994166986223
43	17.80192597473441	25.124086566245214	31.842962306848932	25.231025152171448
44	21.253557868357518	24.269117972703654	28.814223912115928	25.663100246822896
45	21.905343148639233	24.50935172532986	31.543966341350128	22.04133878468078
46	18.793862373280476	25.487245683299758	33.37488455493565	22.34400738848412
47	19.188887028565567	25.512630095110527	31.079053539505164	24.21942933681874
48	19.072226753009673	23.862751346183966	34.92398178803476	22.1410401127716
49	19.950743439209734	23.943009295015475	31.80720809276652	24.299039173008268
50	19.080652217355375	23.127143497539873	30.251521714473977	27.540682570630775
51	19.826305811950117	22.611785928394355	33.9299930327891	23.63191522686643
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1692.0
1	1308.5
2	925.0
3	656.0
4	387.0
5	408.5
6	430.0
7	485.5
8	541.0
9	721.0
10	901.0
11	1012.0
12	1123.0
13	1220.5
14	1318.0
15	1444.5
16	1571.0
17	2003.5
18	2436.0
19	3210.0
20	3984.0
21	4546.5
22	5109.0
23	6795.0
24	8481.0
25	10406.0
26	13849.5
27	15368.0
28	18439.0
29	21510.0
30	24231.5
31	26953.0
32	29043.5
33	31134.0
34	34396.0
35	37658.0
36	39555.5
37	41453.0
38	41425.5
39	41398.0
40	44636.5
41	47875.0
42	50059.0
43	52243.0
44	60272.0
45	68301.0
46	78561.0
47	88821.0
48	97586.5
49	106352.0
50	96741.0
51	87130.0
52	74415.5
53	61701.0
54	51513.0
55	41325.0
56	35251.5
57	29178.0
58	26357.0
59	23536.0
60	20218.5
61	16901.0
62	15047.5
63	13194.0
64	11790.0
65	10386.0
66	8582.5
67	6779.0
68	5726.0
69	4673.0
70	3849.5
71	3026.0
72	2668.0
73	2310.0
74	1868.5
75	1479.0
76	1531.0
77	1309.0
78	1087.0
79	958.5
80	830.0
81	533.5
82	237.0
83	152.5
84	68.0
85	54.5
86	41.0
87	40.0
88	39.0
89	35.5
90	32.0
91	25.5
92	19.0
93	16.0
94	13.0
95	9.0
96	5.0
97	2.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	925765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.100953065646074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90716457553317	24.463975858297037
2	10.785921376791716	7.140485545258627
3	4.055330532920222	4.027059169076213
4	2.0951205351838826	2.7740194600797192
5	1.3445682137400403	2.2253244668284324
6	0.9773887619215592	1.9411499721153276
7	0.7213358034574646	1.6713831802370949
8	0.5597870113960675	1.4823586872783612
9	0.46619488371824136	1.3888345468861654
>10	4.344305452178808	30.569152448964854
>50	0.6235028710587779	13.988195689569501
>100	0.11306182148756222	5.650665321618006
>500	0.0036578824598901146	0.8151945490862225
>1k	0.0023277433835664366	0.9897690231411441
>5k	3.325347690809195E-4	0.872432081563246
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	7926	0.856156800051849	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1664	0.17974323937500336	TruSeq Adapter, Index 20 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	1664	0.17974323937500336	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1385	0.1496060015230647	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGT	1139	0.12303338320200051	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC	1102	0.11903668857647458	No Hit
TACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	1024	0.1106112242307713	No Hit
ACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1014	0.10953103649414268	TruSeq Adapter, Index 22 (95% over 21bp)
AACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	990	0.10693858592623398	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0801877366286261E-4	0.0	0.0	0.9637434986200601	0.0
2	1.0801877366286261E-4	0.0	0.0	1.3965747246871507	0.0
3	1.0801877366286261E-4	0.0	0.0	1.9045870172235935	0.0
4	1.0801877366286261E-4	0.0	0.0	4.0671228659541026	0.0
5	1.0801877366286261E-4	0.0	0.0	4.37983721570809	0.0
6	1.0801877366286261E-4	0.0	0.0	6.160958774635032	0.0
7	1.0801877366286261E-4	0.0	0.0	7.485592996062715	0.0
8	1.0801877366286261E-4	0.0	0.0	8.727052761769995	0.0
9	1.0801877366286261E-4	0.0	0.0	10.429860709791361	0.0
10	1.0801877366286261E-4	0.0	0.0	13.045967389132231	0.0
11	1.0801877366286261E-4	0.0	0.0	15.242313114019217	0.0
12	1.0801877366286261E-4	0.0	0.0	16.289015030812354	0.0
13	1.0801877366286261E-4	0.0	0.0	16.709964191776532	0.0
14	1.0801877366286261E-4	0.0	0.0	17.103800640551327	0.0
15	1.0801877366286261E-4	0.0	0.0	17.457994199391855	0.0
16	1.0801877366286261E-4	0.0	0.0	18.256793030628724	0.0
17	1.0801877366286261E-4	0.0	0.0	19.384833083989996	0.0
18	1.0801877366286261E-4	0.0	0.0	20.69844939050407	0.0
19	1.0801877366286261E-4	0.0	0.0	21.324418183880358	0.0
20	1.0801877366286261E-4	0.0	0.0	21.960864798301944	0.0
21	1.0801877366286261E-4	0.0	0.0	22.62485620000756	0.0
22	1.0801877366286261E-4	0.0	0.0	23.397730525565343	0.0
23	1.0801877366286261E-4	0.0	0.0	24.15494212894201	0.0
24	1.0801877366286261E-4	0.0	0.0	24.75682273579148	0.0
25	1.0801877366286261E-4	0.0	0.0	25.29000340259137	0.0
26	1.0801877366286261E-4	0.0	0.0	25.754376110568018	0.0
27	1.0801877366286261E-4	0.0	0.0	26.283344045195054	0.0
28	1.0801877366286261E-4	0.0	0.0	26.774505409040092	0.0
29	1.0801877366286261E-4	0.0	0.0	27.313195033296786	0.0
30	1.0801877366286261E-4	0.0	0.0	28.020501963241212	0.0
31	1.0801877366286261E-4	0.0	0.0	28.51933266001631	0.0
32	1.0801877366286261E-4	0.0	0.0	29.007361479425125	0.0
33	1.0801877366286261E-4	0.0	0.0	29.47691908853759	0.0
34	1.0801877366286261E-4	0.0	0.0	29.926709262069746	0.0
35	1.0801877366286261E-4	0.0	0.0	30.4079328987378	0.0
36	1.0801877366286261E-4	0.0	0.0	30.854050433965423	0.0
37	1.0801877366286261E-4	0.0	0.0	31.308377395991425	0.0
38	1.0801877366286261E-4	0.0	0.0	31.755575118955676	0.0
39	1.0801877366286261E-4	0.0	0.0	32.2365827180764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGCG	35	1.2115925E-7	45.0	1
CGACTAG	65	0.0	41.538464	1
TTCACGG	170	0.0	41.02941	2
CCCAACG	55	6.002665E-11	40.909092	1
ACGACAG	50	1.0804797E-9	40.5	1
CTATCCG	40	3.457244E-7	39.375	1
CGGCACG	40	3.457244E-7	39.375	1
CAGAACG	70	0.0	38.571426	1
TAGTACG	35	6.2469735E-6	38.571426	1
AATGCGG	195	0.0	38.076923	2
ACGGGAC	530	0.0	37.783016	5
GCGAAGG	280	0.0	37.767857	2
GTTCGCA	30	1.13975024E-4	37.500004	14
CTAGGGA	705	0.0	37.340427	4
GTACGGG	285	0.0	37.105263	3
ACTAGGG	790	0.0	37.025314	3
TAGGGCA	395	0.0	37.025314	5
CCACGGG	650	0.0	36.692307	3
CGAGTTA	80	0.0	36.5625	25
CAACGGG	745	0.0	36.543625	3
>>END_MODULE