SRR3553109_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	601626
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	4518	0.7509648851612132	Illumina Paired End PCR Primer 2 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	2028	0.3370864955969323	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	1920	0.3191351437604093	RNA PCR Primer, Index 23 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGT	1781	0.29603108908192133	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1156	0.19214595113907976	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	823	0.1367959496431338	RNA PCR Primer, Index 1 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	30	2.1637698E-6	45.000004	1
AGCGTTC	20	7.0306024E-4	45.0	12
CGTGCAT	20	7.0306024E-4	45.0	15
TCGAGTG	20	7.0306024E-4	45.0	29
TGGTACC	20	7.0306024E-4	45.0	13
CACGGGT	200	0.0	41.625	4
ATGGGAC	275	0.0	40.909092	5
TAGCGCG	45	1.925764E-8	40.000004	1
GTACTGG	90	0.0	40.000004	2
CACTAGG	120	0.0	39.375004	2
AGTCCAC	40	3.454552E-7	39.375	31
CACGCTA	40	3.454552E-7	39.375	13
CACGAAG	40	3.454552E-7	39.375	1
CGATAGG	150	0.0	39.000004	2
CGGGCTC	185	0.0	38.91892	6
TGGGACG	145	0.0	38.793102	6
TGGGACC	180	0.0	38.750004	6
ATAGCGG	105	0.0	38.57143	2
TGATCGG	35	6.2433264E-6	38.571426	25
AGCCACG	70	0.0	38.571426	1