##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	601626
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.665767436912635	31.0	31.0	34.0	30.0	34.0
2	31.87117411813984	33.0	31.0	34.0	30.0	34.0
3	32.03279113602138	33.0	31.0	34.0	30.0	34.0
4	35.711874154374975	37.0	35.0	37.0	33.0	37.0
5	35.482557269798846	37.0	35.0	37.0	33.0	37.0
6	35.51678950045377	37.0	35.0	37.0	33.0	37.0
7	35.683700504964875	37.0	35.0	37.0	35.0	37.0
8	35.745331817441404	37.0	35.0	37.0	35.0	37.0
9	37.42170218707303	39.0	37.0	39.0	35.0	39.0
10	36.932421471146526	39.0	37.0	39.0	33.0	39.0
11	36.91684368694172	39.0	37.0	39.0	33.0	39.0
12	36.872193023572784	39.0	37.0	39.0	33.0	39.0
13	36.8622034287082	39.0	37.0	39.0	33.0	39.0
14	38.0170072437029	40.0	37.0	41.0	33.0	41.0
15	38.01686263559088	40.0	37.0	41.0	33.0	41.0
16	37.93879253888628	40.0	37.0	41.0	33.0	41.0
17	37.8926525781798	40.0	37.0	41.0	33.0	41.0
18	37.83706156316383	40.0	37.0	41.0	33.0	41.0
19	37.890259064601594	40.0	37.0	41.0	33.0	41.0
20	37.83502707662235	40.0	37.0	41.0	33.0	41.0
21	37.806835143427975	39.0	37.0	41.0	33.0	41.0
22	37.78425965633134	39.0	37.0	41.0	33.0	41.0
23	37.68047424812092	39.0	37.0	41.0	33.0	41.0
24	37.54306994711	39.0	36.0	41.0	32.0	41.0
25	37.593398556578336	39.0	36.0	41.0	32.0	41.0
26	37.54774062291191	39.0	36.0	41.0	32.0	41.0
27	37.47758241831304	39.0	36.0	41.0	32.0	41.0
28	37.45290429602444	39.0	36.0	41.0	32.0	41.0
29	37.304080940650834	39.0	36.0	41.0	32.0	41.0
30	37.247595682367454	39.0	36.0	41.0	31.0	41.0
31	37.26187365572631	39.0	36.0	41.0	32.0	41.0
32	37.15328792306184	39.0	36.0	41.0	31.0	41.0
33	37.07140482625419	39.0	36.0	41.0	31.0	41.0
34	37.084349745522964	39.0	36.0	41.0	31.0	41.0
35	36.97999587783772	39.0	35.0	41.0	31.0	41.0
36	36.9283325521171	39.0	35.0	41.0	31.0	41.0
37	36.80991845432212	39.0	35.0	40.0	31.0	41.0
38	36.77767416966687	39.0	35.0	40.0	31.0	41.0
39	36.67967641026152	39.0	35.0	40.0	31.0	41.0
40	36.58394085362002	39.0	35.0	40.0	30.0	41.0
41	36.52497066283704	39.0	35.0	40.0	30.0	41.0
42	36.47216376951794	39.0	35.0	40.0	30.0	41.0
43	36.37867711834262	39.0	35.0	40.0	30.0	41.0
44	36.27735669668532	38.0	35.0	40.0	30.0	41.0
45	36.073065326299066	38.0	35.0	40.0	29.0	41.0
46	35.89001472675716	38.0	35.0	40.0	29.0	41.0
47	35.765179696356206	38.0	34.0	40.0	28.0	41.0
48	35.697007775594805	38.0	34.0	40.0	28.0	41.0
49	35.51657009504243	38.0	34.0	40.0	28.0	41.0
50	35.435747457722904	38.0	34.0	40.0	28.0	41.0
51	34.11477396256146	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	5.0
12	2.0
13	13.0
14	17.0
15	23.0
16	56.0
17	102.0
18	174.0
19	323.0
20	616.0
21	990.0
22	1305.0
23	1801.0
24	2304.0
25	2935.0
26	3830.0
27	4989.0
28	6509.0
29	8388.0
30	11090.0
31	14369.0
32	18835.0
33	25493.0
34	37063.0
35	47416.0
36	57986.0
37	84875.0
38	127963.0
39	142113.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.467576201826382	31.959888701618617	21.64318031468055	26.929354781874455
2	21.914445186876897	29.575184583113096	22.762812777373316	25.747557452636688
3	21.213345167928246	29.519502149175736	22.486727634776425	26.780425048119593
4	20.18263838331455	30.235062979326027	20.328243792655236	29.254054844704186
5	17.879047780514806	33.444365768766644	19.66587215313168	29.01071429758687
6	20.269070818082994	35.56029825838644	21.098157326977223	23.07247359655334
7	84.0289482169986	4.779713642694963	6.216652870720348	4.974685269586089
8	85.94641853909239	3.244374412010119	4.644081206596789	6.165125842300698
9	78.1771399507335	6.6812272076007355	9.311100251651357	5.8305325900143945
10	34.260487412445606	27.98200210762168	15.22540581690286	22.532104663029855
11	23.52208847356996	26.348595306718792	29.199203491870364	20.930112727840886
12	22.162938436836175	21.682573558988473	36.714669911207295	19.439818092968057
13	23.814462805796293	24.967172296410062	29.748049452649983	21.47031544514366
14	19.97270729655966	27.775395345280955	30.536246771249914	21.715650586909476
15	18.33614238746331	32.59998736756722	27.96504805310941	21.09882219186006
16	19.166724842343914	30.311688657072665	27.982168323842387	22.539418176741034
17	17.207700465072985	30.7927183998032	30.309361629982746	21.690219505141066
18	19.000009972973242	29.068889974834867	29.800739994614595	22.1303600575773
19	19.025773487183066	32.88471575364096	29.222972411431687	18.86653834774428
20	19.862007293567764	31.072626515476394	29.255384574469858	19.809981616485988
21	18.87900456429742	32.065269785547834	28.420148065409407	20.63557758474534
22	17.79494237283628	30.538075149677706	29.352122414922228	22.314860062563785
23	18.774953210133873	30.729556235933952	26.79904126483895	23.696449289093223
24	15.95260178250275	31.746633290449548	29.40564403799038	22.89512088905732
25	18.570839691103775	30.165418382849147	28.408180497518394	22.855561428528688
26	19.475222147978975	30.52078866272402	27.85567777988318	22.14831140941382
27	17.444059930920538	30.68617380232902	27.871135888409075	23.998630378341364
28	21.155501923121673	29.77697107505327	28.520542662717368	20.546984339107684
29	20.016255946385293	31.114346786874236	26.308370981307323	22.56102628543314
30	20.97299651278369	32.55028871757537	25.61824123292544	20.8584735367155
31	21.78978302134549	30.789061642947612	24.577561475069228	22.84359386063767
32	20.914987051756405	31.32195084653921	25.52648987909432	22.23657222261006
33	20.716358668009693	30.739529209176464	26.909741267830846	21.634370854982997
34	20.468364066712542	27.91551561933826	28.38773590237124	23.228384411577956
35	20.981307323819117	31.282391386010577	26.665902072051406	21.070399218118897
36	20.798635697260423	31.46124003949297	27.929145349436364	19.81097891381024
37	20.452074877083103	33.052095487894476	25.124080408758932	21.371749226263493
38	22.93733980911729	31.475035985811783	27.174357491198855	18.413266713872073
39	20.216546492339095	30.60522650284396	28.71833996536054	20.459887039456408
40	20.039360001063784	31.388437334822633	27.941279133548086	20.6309235305655
41	18.22510995203	28.838680509153527	31.46223733681722	21.473972201999246
42	20.85631272584629	30.242875141699326	27.075625056097973	21.825187076356407
43	18.911582943556297	28.57140482625418	29.445535930960432	23.071476299229086
44	21.745569506637015	27.166379112604837	27.6595426394471	23.428508741311045
45	23.37332495603581	27.63377912523727	28.245122385003306	20.74777353372361
46	18.985382945550892	29.244081871461674	30.78357650766423	20.98695867532321
47	19.800839724347018	29.397000794513534	28.22766968182891	22.574489799310534
48	19.896912699916562	27.40523182176302	31.76591437205174	20.931941106268678
49	20.784008669838073	27.67998723459425	28.115806165292057	23.42019793027562
50	19.229554573771747	26.694657478233985	27.98416291849089	26.09162502950338
51	19.907550538041907	25.472303391143335	31.406056254217734	23.21408981659702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1448.0
1	1080.5
2	713.0
3	494.0
4	275.0
5	320.0
6	365.0
7	373.5
8	382.0
9	462.0
10	542.0
11	607.5
12	673.0
13	764.0
14	855.0
15	1007.0
16	1159.0
17	1268.5
18	1378.0
19	1975.0
20	2572.0
21	3190.5
22	3809.0
23	4376.5
24	4944.0
25	6657.5
26	9957.5
27	11544.0
28	13202.0
29	14860.0
30	17658.0
31	20456.0
32	21531.0
33	22606.0
34	23688.5
35	24771.0
36	26997.0
37	29223.0
38	29657.5
39	30092.0
40	29949.5
41	29807.0
42	31681.5
43	33556.0
44	38155.5
45	42755.0
46	47160.5
47	51566.0
48	55529.5
49	59493.0
50	54932.0
51	50371.0
52	44507.0
53	38643.0
54	32779.0
55	26915.0
56	23168.5
57	19422.0
58	17544.5
59	15667.0
60	14972.0
61	14277.0
62	11844.5
63	9412.0
64	8646.5
65	7881.0
66	6985.0
67	6089.0
68	5280.5
69	4472.0
70	3697.0
71	2922.0
72	2513.5
73	2105.0
74	1946.5
75	1538.0
76	1288.0
77	1173.5
78	1059.0
79	813.0
80	567.0
81	486.0
82	405.0
83	241.0
84	77.0
85	50.5
86	24.0
87	17.5
88	11.0
89	8.0
90	5.0
91	6.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	601626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.044968356628516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01543749204845	25.53898195675434
2	9.83678308083564	6.6978593743613635
3	3.7786734539189117	3.859344545061046
4	2.061761909835652	2.80770475917027
5	1.307359278461309	2.225450263297998
6	0.9475090054031228	1.9354748463941924
7	0.7454606194231397	1.776542823956144
8	0.6192617869767828	1.6866198353675044
9	0.4801926345533716	1.471332874361006
>10	4.548631004570319	32.63309996137409
>50	0.5851689428569496	13.25854822469428
>100	0.07081035947167844	4.06225832634941
>500	4.917386074422114E-4	0.1377802844667449
>1k	0.002458693037211057	1.909001924391606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	4518	0.7509648851612132	Illumina Paired End PCR Primer 2 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	2028	0.3370864955969323	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	1920	0.3191351437604093	RNA PCR Primer, Index 23 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGT	1781	0.29603108908192133	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1156	0.19214595113907976	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	823	0.1367959496431338	RNA PCR Primer, Index 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.868645969422864	0.0
2	0.0	0.0	0.0	1.2145419247173492	0.0
3	0.0	0.0	0.0	1.6538513960500378	0.0
4	0.0	0.0	0.0	3.564839285536197	0.0
5	0.0	0.0	0.0	3.8515622662584397	0.0
6	0.0	0.0	0.0	5.40102987570351	0.0
7	0.0	0.0	0.0	6.531965041404461	0.0
8	0.0	0.0	0.0	7.591427232200736	0.0
9	0.0	0.0	0.0	9.204057005515054	0.0
10	0.0	0.0	0.0	11.41722598424935	0.0
11	0.0	0.0	0.0	13.558423339416848	0.0
12	0.0	0.0	0.0	14.43288687656451	0.0
13	0.0	0.0	0.0	14.795570670150559	0.0
14	0.0	0.0	0.0	15.17055446406904	0.0
15	0.0	0.0	0.0	15.490188256491574	0.0
16	0.0	0.0	0.0	16.196773410723605	0.0
17	0.0	0.0	0.0	17.22432208714384	0.0
18	0.0	0.0	0.0	18.38617346989658	1.662162207085465E-4
19	1.662162207085465E-4	0.0	0.0	18.959619431341064	1.662162207085465E-4
20	1.662162207085465E-4	0.0	0.0	19.505141067706514	1.662162207085465E-4
21	1.662162207085465E-4	0.0	0.0	20.110334327306333	1.662162207085465E-4
22	1.662162207085465E-4	0.0	0.0	20.77619650746477	1.662162207085465E-4
23	1.662162207085465E-4	0.0	0.0	21.449704633775802	1.662162207085465E-4
24	1.662162207085465E-4	0.0	0.0	21.977607350746144	1.662162207085465E-4
25	1.662162207085465E-4	0.0	0.0	22.44816547157204	1.662162207085465E-4
26	1.662162207085465E-4	0.0	0.0	22.866033050433327	1.662162207085465E-4
27	1.662162207085465E-4	0.0	0.0	23.33093981975513	1.662162207085465E-4
28	1.662162207085465E-4	0.0	0.0	23.73717226316682	1.662162207085465E-4
29	1.662162207085465E-4	0.0	0.0	24.18728578884556	1.662162207085465E-4
30	1.662162207085465E-4	0.0	0.0	24.791647967341838	1.662162207085465E-4
31	1.662162207085465E-4	0.0	0.0	25.224973654729016	1.662162207085465E-4
32	1.662162207085465E-4	0.0	0.0	25.63286826034779	1.662162207085465E-4
33	1.662162207085465E-4	0.0	0.0	26.0550574609475	1.662162207085465E-4
34	3.32432441417093E-4	0.0	0.0	26.43685611991503	1.662162207085465E-4
35	3.32432441417093E-4	0.0	0.0	26.85971018539757	1.662162207085465E-4
36	3.32432441417093E-4	0.0	0.0	27.238184519950934	1.662162207085465E-4
37	3.32432441417093E-4	0.0	0.0	27.64308723359695	1.662162207085465E-4
38	3.32432441417093E-4	0.0	0.0	28.051979136539977	1.662162207085465E-4
39	3.32432441417093E-4	0.0	0.0	28.46452779633859	1.662162207085465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	30	2.1637698E-6	45.000004	1
AGCGTTC	20	7.0306024E-4	45.0	12
CGTGCAT	20	7.0306024E-4	45.0	15
TCGAGTG	20	7.0306024E-4	45.0	29
TGGTACC	20	7.0306024E-4	45.0	13
CACGGGT	200	0.0	41.625	4
ATGGGAC	275	0.0	40.909092	5
TAGCGCG	45	1.925764E-8	40.000004	1
GTACTGG	90	0.0	40.000004	2
CACTAGG	120	0.0	39.375004	2
AGTCCAC	40	3.454552E-7	39.375	31
CACGCTA	40	3.454552E-7	39.375	13
CACGAAG	40	3.454552E-7	39.375	1
CGATAGG	150	0.0	39.000004	2
CGGGCTC	185	0.0	38.91892	6
TGGGACG	145	0.0	38.793102	6
TGGGACC	180	0.0	38.750004	6
ATAGCGG	105	0.0	38.57143	2
TGATCGG	35	6.2433264E-6	38.571426	25
AGCCACG	70	0.0	38.571426	1
>>END_MODULE