Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553108_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 711655 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC | 10934 | 1.5364186298136036 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGT | 10896 | 1.531078963823763 | No Hit |
AATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT | 9141 | 1.284470705608757 | No Hit |
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT | 5754 | 0.808537844882703 | Illumina Single End Adapter 1 (95% over 21bp) |
AATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT | 2579 | 0.36239469967891746 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT | 2128 | 0.29902129543107264 | No Hit |
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT | 1774 | 0.24927809120992617 | No Hit |
AACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG | 1728 | 0.2428142850116981 | No Hit |
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGATC | 1720 | 0.24169014480331058 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1156 | 0.16243826011199247 | No Hit |
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC | 1116 | 0.156817559070055 | No Hit |
AATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC | 780 | 0.10960367031778039 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTGTCGG | 20 | 7.031474E-4 | 45.0 | 2 |
CGCTCTA | 35 | 1.211065E-7 | 45.0 | 32 |
CACTACG | 35 | 1.211065E-7 | 45.0 | 1 |
CGTATAG | 30 | 2.1643318E-6 | 44.999996 | 1 |
TGCGTAG | 30 | 2.1643318E-6 | 44.999996 | 1 |
TAGGGTG | 115 | 0.0 | 41.08696 | 5 |
TAGGGAC | 335 | 0.0 | 40.97015 | 5 |
AATACGG | 95 | 0.0 | 40.263157 | 2 |
TACACGC | 280 | 0.0 | 40.17857 | 35 |
CATACCG | 40 | 3.4557524E-7 | 39.375 | 1 |
TGGGCCG | 75 | 0.0 | 39.0 | 6 |
TACGGCT | 1400 | 0.0 | 38.410717 | 6 |
CACTAGG | 190 | 0.0 | 37.894737 | 2 |
GTCTTAC | 315 | 0.0 | 37.857143 | 31 |
TAAGGGA | 345 | 0.0 | 37.826084 | 4 |
ACACGCG | 300 | 0.0 | 37.5 | 36 |
AACGCAC | 30 | 1.1394851E-4 | 37.499996 | 30 |
CCATAGG | 175 | 0.0 | 37.285717 | 2 |
CTAGGGT | 140 | 0.0 | 36.964283 | 4 |
ATAGGGC | 375 | 0.0 | 36.600002 | 4 |