FastQCFastQC Report
Sat 18 Jun 2016
SRR3553108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711655
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC109341.5364186298136036No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGT108961.531078963823763No Hit
AATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT91411.284470705608757No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT57540.808537844882703Illumina Single End Adapter 1 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT25790.36239469967891746No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT21280.29902129543107264No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT17740.24927809120992617No Hit
AACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG17280.2428142850116981No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGATC17200.24169014480331058No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11560.16243826011199247No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC11160.156817559070055No Hit
AATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC7800.10960367031778039No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCGG207.031474E-445.02
CGCTCTA351.211065E-745.032
CACTACG351.211065E-745.01
CGTATAG302.1643318E-644.9999961
TGCGTAG302.1643318E-644.9999961
TAGGGTG1150.041.086965
TAGGGAC3350.040.970155
AATACGG950.040.2631572
TACACGC2800.040.1785735
CATACCG403.4557524E-739.3751
TGGGCCG750.039.06
TACGGCT14000.038.4107176
CACTAGG1900.037.8947372
GTCTTAC3150.037.85714331
TAAGGGA3450.037.8260844
ACACGCG3000.037.536
AACGCAC301.1394851E-437.49999630
CCATAGG1750.037.2857172
CTAGGGT1400.036.9642834
ATAGGGC3750.036.6000024