##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	711655
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63036724255433	31.0	31.0	34.0	30.0	34.0
2	31.8338281892209	33.0	31.0	34.0	30.0	34.0
3	31.98915485733958	33.0	31.0	34.0	30.0	34.0
4	35.66013869079821	37.0	35.0	37.0	33.0	37.0
5	35.40773267945845	37.0	35.0	37.0	33.0	37.0
6	34.76292304557686	37.0	35.0	37.0	32.0	37.0
7	35.1463967793383	37.0	35.0	37.0	32.0	37.0
8	35.62655921759841	37.0	35.0	37.0	33.0	37.0
9	37.341841201143815	39.0	37.0	39.0	34.0	39.0
10	36.87356514041214	39.0	37.0	39.0	33.0	39.0
11	36.854897387076605	39.0	37.0	39.0	33.0	39.0
12	36.68737379769692	39.0	35.0	39.0	32.0	39.0
13	36.7444660685304	39.0	35.0	39.0	32.0	39.0
14	37.83061174304965	40.0	37.0	41.0	33.0	41.0
15	37.89008578594965	40.0	37.0	41.0	33.0	41.0
16	37.71074607780455	40.0	36.0	41.0	32.0	41.0
17	37.72198607471317	39.0	37.0	41.0	33.0	41.0
18	37.65217275224652	39.0	36.0	41.0	32.0	41.0
19	37.65630115716183	39.0	36.0	41.0	32.0	41.0
20	37.68510162930072	39.0	36.0	41.0	32.0	41.0
21	37.74003555093409	39.0	37.0	41.0	33.0	41.0
22	37.7056593433616	39.0	36.0	41.0	33.0	41.0
23	37.622463131714106	39.0	36.0	41.0	32.0	41.0
24	37.486849667324755	39.0	36.0	41.0	32.0	41.0
25	37.53305885576579	39.0	36.0	41.0	32.0	41.0
26	37.46234622113243	39.0	36.0	41.0	32.0	41.0
27	37.400718044558104	39.0	36.0	41.0	32.0	41.0
28	37.320638511638364	39.0	36.0	41.0	32.0	41.0
29	37.13359984824107	39.0	36.0	41.0	31.0	41.0
30	37.21328452691262	39.0	36.0	41.0	31.0	41.0
31	37.14416395584939	39.0	36.0	41.0	31.0	41.0
32	37.11101025075352	39.0	36.0	41.0	31.0	41.0
33	36.87904110840225	39.0	35.0	40.0	31.0	41.0
34	36.874021822371795	39.0	35.0	40.0	31.0	41.0
35	36.75904616703318	39.0	35.0	40.0	31.0	41.0
36	36.80789708496392	39.0	35.0	40.0	31.0	41.0
37	36.755221279974144	39.0	35.0	40.0	31.0	41.0
38	36.61967245364678	39.0	35.0	40.0	30.0	41.0
39	36.56888660938235	39.0	35.0	40.0	30.0	41.0
40	36.46448489787889	39.0	35.0	40.0	30.0	41.0
41	36.471729981521946	39.0	35.0	40.0	30.0	41.0
42	36.376490012716836	39.0	35.0	40.0	30.0	41.0
43	36.366709992903864	39.0	35.0	40.0	30.0	41.0
44	36.30476284154541	38.0	35.0	40.0	30.0	41.0
45	36.0662090479235	38.0	35.0	40.0	29.0	41.0
46	35.83455747518109	38.0	34.0	40.0	28.0	41.0
47	35.80903527692492	38.0	34.0	40.0	29.0	41.0
48	35.71983756173989	38.0	34.0	40.0	28.0	41.0
49	35.62520884417309	38.0	34.0	40.0	28.0	41.0
50	35.537728253156374	38.0	34.0	40.0	28.0	41.0
51	34.12637443705166	36.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	4.0
11	5.0
12	6.0
13	4.0
14	19.0
15	26.0
16	45.0
17	97.0
18	196.0
19	466.0
20	735.0
21	1278.0
22	1883.0
23	2432.0
24	3000.0
25	3572.0
26	4510.0
27	5492.0
28	7425.0
29	9758.0
30	13219.0
31	18049.0
32	24332.0
33	32701.0
34	47449.0
35	59090.0
36	70869.0
37	99575.0
38	145320.0
39	160040.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.259191602672644	36.26616829784095	19.832362591424214	24.642277508062193
2	19.46870323401086	34.53597599960655	20.562070104193744	25.43325066218884
3	19.922855878199407	27.288925111184493	27.133934279953063	25.65428473066303
4	23.92985365099662	27.383352888689043	20.26220570360638	28.424587756707954
5	17.285622949322356	35.235893796853816	19.88786701421335	27.590616239610487
6	20.04482509080945	33.38794781179082	24.64438527095292	21.922841826446803
7	77.73457644504711	9.246896319143405	7.561248076666362	5.457279159143124
8	80.61616935172239	3.4258172850608797	5.069029234671294	10.888984128545433
9	75.6684067420309	6.356872360905213	10.520266140194336	7.454454756869551
10	34.37578601991133	26.104783919174317	16.781305548334515	22.738124512579834
11	23.579262423505774	25.316902150620734	28.963612986629755	22.140222439243733
12	22.652549339216332	21.082828055729248	38.24844903780624	18.016173567248174
13	24.586773085273062	25.237650265929418	27.852962460742916	22.322614188054605
14	18.639649830325087	26.80849568962489	33.164243910321716	21.38761056972831
15	18.05537795701569	31.32444794176954	27.081802277789098	23.538371823425678
16	18.705974102619948	29.85210530383402	29.472567465977196	21.969353127568837
17	17.08931996543269	30.035199640275135	29.95552620300567	22.91995419128651
18	18.196879105746465	29.50966409285398	28.916961167981675	23.37649563341788
19	18.639087760220892	32.224603213635824	31.8984620356774	17.237846990465886
20	19.46252046286473	32.949392612993655	28.566229422964778	19.021857501176832
21	18.35875529575426	30.12344464663355	28.995932017620895	22.52186803999129
22	17.29686435140623	32.256781727100915	29.449803626757348	20.99655029473551
23	19.81311169035558	28.71953404388362	26.605447864484898	24.8619064012759
24	16.12031110580267	33.72139590110377	27.358481286578467	22.799811706515094
25	17.807364523540198	29.615333272442406	28.22673908003176	24.350563123985637
26	20.145154604408035	31.785907497312603	26.199914284309113	21.869023613970253
27	16.25605103596546	29.612382404395387	30.54991533819055	23.581651221448595
28	20.40356633481111	31.160885541449158	26.926811446557675	21.50873667718206
29	19.182609550976245	34.87883876316474	26.244739375118563	19.69381231074046
30	20.350029157386654	29.304930057401407	27.39501584335106	22.950024941860875
31	23.457152693369682	32.94363139442567	23.36441112617771	20.234804786026935
32	22.09019819997049	29.160478040623616	25.640092460532138	23.109231298873752
33	21.644898159922995	29.680111851950734	26.5018864477872	22.173103540339067
34	21.49988407304101	30.647293983742124	26.417856967210234	21.434964976006633
35	18.24058005634753	33.33918823025202	27.936710906267788	20.483520807132667
36	21.183860156958076	31.226647743639823	29.032888126971635	18.55660397243046
37	22.607583730880833	29.928125285426226	27.034588389036823	20.429702594656117
38	24.532392802692314	28.709276264482085	26.36038529905643	20.397945633769172
39	23.84905607351877	26.90460967744202	27.2793699194132	21.966964329626013
40	21.64981627333469	30.339139049117904	24.579606691444592	23.431437986102814
41	20.607597782633437	28.563278554917765	29.878663116257176	20.950460546191625
42	21.227139554980994	31.68487539608378	25.881782605335452	21.20620244359978
43	19.598822463131714	28.117556962292124	29.540858983636735	22.74276159093943
44	21.42161581103203	25.168796678165684	28.430208457749895	24.97937905305239
45	24.553189396547484	28.0612094343467	26.878754452649105	20.50684671645671
46	18.06507366631303	26.53153564578342	34.00706803156023	21.39632265634331
47	20.63542025279103	28.79695920073631	26.673036794514193	23.89458375195846
48	20.11424074867738	25.798877264966873	32.855245870541204	21.231636115814545
49	21.429625310016792	25.585290625373247	28.79681868321026	24.188265381399695
50	20.08950966409285	24.935959137503424	27.20264735019075	27.77188384821297
51	19.190759567487053	23.936598492246947	33.110566215371215	23.76207572489479
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1394.0
1	1133.5
2	873.0
3	619.0
4	365.0
5	405.5
6	446.0
7	498.5
8	551.0
9	613.5
10	676.0
11	825.5
12	975.0
13	1088.5
14	1202.0
15	1306.0
16	1410.0
17	1830.0
18	2250.0
19	2514.5
20	2779.0
21	3456.5
22	4134.0
23	5177.5
24	6221.0
25	7792.5
26	10570.0
27	11776.0
28	14114.5
29	16453.0
30	17988.5
31	19524.0
32	21676.0
33	23828.0
34	25788.0
35	27748.0
36	29496.0
37	31244.0
38	32307.5
39	33371.0
40	33667.0
41	33963.0
42	35466.5
43	36970.0
44	53477.5
45	69985.0
46	71188.0
47	72391.0
48	71195.5
49	70000.0
50	63204.0
51	56408.0
52	49771.5
53	43135.0
54	37151.5
55	31168.0
56	27595.5
57	24023.0
58	21688.5
59	19354.0
60	17884.0
61	16414.0
62	14948.5
63	13483.0
64	11044.0
65	8605.0
66	7163.5
67	5722.0
68	4873.0
69	4024.0
70	3348.0
71	2672.0
72	2462.5
73	2253.0
74	1922.0
75	1409.0
76	1227.0
77	1000.5
78	774.0
79	589.0
80	404.0
81	374.0
82	344.0
83	215.0
84	86.0
85	63.0
86	40.0
87	31.5
88	23.0
89	14.5
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	711655.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.87410139814091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72972316858385	25.314022200676096
2	10.003749857308009	6.7773607405617815
3	3.74710892864128	3.807898433960216
4	2.0540324144361874	2.783140091267184
5	1.3151164657569678	2.2274194255708117
6	0.9941962680312832	2.020650311576698
7	0.7379522213867807	1.7498227861967395
8	0.6034680292224055	1.6353549769932818
9	0.517310257239169	1.5771078097216173
>10	4.933146946151527	35.251275177572374
>50	0.3127101083137537	6.56852049475341
>100	0.04431114891412034	2.7456870237192703
>500	0.0025320656522354475	0.5476506996339109
>1k	0.002954076594274689	1.744162408309409
>5k	8.440218840784825E-4	2.1292762127504834
>10k+	8.440218840784825E-4	3.120651206736693
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	10934	1.5364186298136036	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGT	10896	1.531078963823763	No Hit
AATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	9141	1.284470705608757	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	5754	0.808537844882703	Illumina Single End Adapter 1 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	2579	0.36239469967891746	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	2128	0.29902129543107264	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT	1774	0.24927809120992617	No Hit
AACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1728	0.2428142850116981	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGATC	1720	0.24169014480331058	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1156	0.16243826011199247	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1116	0.156817559070055	No Hit
AATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	780	0.10960367031778039	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.215525781453092E-4	0.0	0.0	0.9146285770492725	0.0
2	4.215525781453092E-4	0.0	0.0	1.2983819406875523	0.0
3	4.215525781453092E-4	0.0	0.0	1.9839669502778734	0.0
4	4.215525781453092E-4	0.0	0.0	4.985561824198523	0.0
5	4.215525781453092E-4	0.0	0.0	5.363835004320914	0.0
6	4.215525781453092E-4	0.0	0.0	7.138430840786617	0.0
7	4.215525781453092E-4	0.0	0.0	8.434845536109492	0.0
8	4.215525781453092E-4	0.0	0.0	9.293267102739389	0.0
9	4.215525781453092E-4	0.0	0.0	12.150128924830149	0.0
10	4.215525781453092E-4	0.0	0.0	14.133112252425684	0.0
11	4.215525781453092E-4	0.0	0.0	17.590545980847462	0.0
12	4.215525781453092E-4	0.0	0.0	18.532856510528276	0.0
13	4.215525781453092E-4	0.0	0.0	18.977734997997626	0.0
14	4.215525781453092E-4	0.0	0.0	19.61427939099704	0.0
15	4.215525781453092E-4	0.0	0.0	19.989461185546368	0.0
16	4.215525781453092E-4	0.0	0.0	20.69036260547597	0.0
17	4.215525781453092E-4	0.0	0.0	21.640682634141545	0.0
18	4.215525781453092E-4	0.0	0.0	22.967027562512733	0.0
19	4.215525781453092E-4	0.0	0.0	23.562119285327864	0.0
20	4.215525781453092E-4	0.0	0.0	24.09439967399934	0.0
21	4.215525781453092E-4	0.0	0.0	24.720405252545124	0.0
22	4.215525781453092E-4	0.0	0.0	25.36861260020656	0.0
23	4.215525781453092E-4	0.0	0.0	26.001784572580814	0.0
24	4.215525781453092E-4	0.0	0.0	26.548257231383186	0.0
25	4.215525781453092E-4	0.0	0.0	26.998475384842376	0.0
26	4.215525781453092E-4	0.0	0.0	27.388833072204932	0.0
27	4.215525781453092E-4	0.0	0.0	27.835257252460814	0.0
28	4.215525781453092E-4	0.0	0.0	28.255826207923782	0.0
29	4.215525781453092E-4	0.0	0.0	28.68229689948079	0.0
30	4.215525781453092E-4	0.0	0.0	29.277248104769868	0.0
31	4.215525781453092E-4	0.0	0.0	29.697957577758885	0.0
32	4.215525781453092E-4	0.0	0.0	30.124006716737746	0.0
33	4.215525781453092E-4	0.0	0.0	30.51169457110538	0.0
34	4.215525781453092E-4	0.0	0.0	30.910342792504796	0.0
35	4.215525781453092E-4	0.0	0.0	31.319951380935986	0.0
36	4.215525781453092E-4	0.0	0.0	31.714384076553948	0.0
37	4.215525781453092E-4	0.0	0.0	32.12371162993304	0.0
38	4.215525781453092E-4	0.0	0.0	32.527840034848346	0.0
39	4.215525781453092E-4	0.0	0.0	32.94854950783736	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCGG	20	7.031474E-4	45.0	2
CGCTCTA	35	1.211065E-7	45.0	32
CACTACG	35	1.211065E-7	45.0	1
CGTATAG	30	2.1643318E-6	44.999996	1
TGCGTAG	30	2.1643318E-6	44.999996	1
TAGGGTG	115	0.0	41.08696	5
TAGGGAC	335	0.0	40.97015	5
AATACGG	95	0.0	40.263157	2
TACACGC	280	0.0	40.17857	35
CATACCG	40	3.4557524E-7	39.375	1
TGGGCCG	75	0.0	39.0	6
TACGGCT	1400	0.0	38.410717	6
CACTAGG	190	0.0	37.894737	2
GTCTTAC	315	0.0	37.857143	31
TAAGGGA	345	0.0	37.826084	4
ACACGCG	300	0.0	37.5	36
AACGCAC	30	1.1394851E-4	37.499996	30
CCATAGG	175	0.0	37.285717	2
CTAGGGT	140	0.0	36.964283	4
ATAGGGC	375	0.0	36.600002	4
>>END_MODULE