Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553107_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 732415 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC | 12689 | 1.7324877289514824 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGT | 12196 | 1.665176163786924 | No Hit |
AATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT | 10387 | 1.4181850453636258 | No Hit |
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT | 6299 | 0.8600315394960508 | Illumina Single End Adapter 1 (95% over 21bp) |
AATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT | 2889 | 0.3944485025566107 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT | 2184 | 0.29819159902514286 | No Hit |
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT | 2046 | 0.27934982216366405 | No Hit |
AACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG | 2009 | 0.2742980414109487 | No Hit |
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGATC | 1800 | 0.24576230688885398 | No Hit |
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC | 1201 | 0.1639780725408409 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1173 | 0.16015510332256985 | No Hit |
AATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC | 895 | 0.12219848036973573 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCATAG | 30 | 2.1644191E-6 | 45.000004 | 1 |
CACTACG | 35 | 1.2111195E-7 | 45.0 | 1 |
GCACTAG | 35 | 1.2111195E-7 | 45.0 | 1 |
TACGGGT | 80 | 0.0 | 42.187504 | 4 |
ACGGGTA | 125 | 0.0 | 41.399998 | 5 |
ACACTAG | 60 | 3.6379788E-12 | 41.250004 | 1 |
GCGGGTT | 150 | 0.0 | 40.5 | 5 |
CGGGCTA | 140 | 0.0 | 40.17857 | 6 |
TACGGCT | 1610 | 0.0 | 40.108696 | 6 |
AACACGG | 230 | 0.0 | 40.108692 | 2 |
CACGCAG | 45 | 1.9266736E-8 | 40.0 | 1 |
TATGCGG | 40 | 3.4559343E-7 | 39.375004 | 2 |
TGTTCGG | 40 | 3.4559343E-7 | 39.375004 | 2 |
GTACGCG | 40 | 3.4559343E-7 | 39.375004 | 1 |
CGACCGG | 35 | 6.2451854E-6 | 38.571426 | 2 |
CCCGACG | 35 | 6.2451854E-6 | 38.571426 | 1 |
GTACGGG | 170 | 0.0 | 38.382355 | 3 |
CTAGGGT | 160 | 0.0 | 37.968754 | 4 |
CTATGCG | 30 | 1.1395175E-4 | 37.500004 | 1 |
GCGTTGA | 30 | 1.1395175E-4 | 37.500004 | 9 |