FastQCFastQC Report
Sat 18 Jun 2016
SRR3553107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732415
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC126891.7324877289514824No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGT121961.665176163786924No Hit
AATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT103871.4181850453636258No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT62990.8600315394960508Illumina Single End Adapter 1 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT28890.3944485025566107No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT21840.29819159902514286No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT20460.27934982216366405No Hit
AACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG20090.2742980414109487No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGATC18000.24576230688885398No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC12010.1639780725408409No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11730.16015510332256985No Hit
AATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC8950.12219848036973573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG302.1644191E-645.0000041
CACTACG351.2111195E-745.01
GCACTAG351.2111195E-745.01
TACGGGT800.042.1875044
ACGGGTA1250.041.3999985
ACACTAG603.6379788E-1241.2500041
GCGGGTT1500.040.55
CGGGCTA1400.040.178576
TACGGCT16100.040.1086966
AACACGG2300.040.1086922
CACGCAG451.9266736E-840.01
TATGCGG403.4559343E-739.3750042
TGTTCGG403.4559343E-739.3750042
GTACGCG403.4559343E-739.3750041
CGACCGG356.2451854E-638.5714262
CCCGACG356.2451854E-638.5714261
GTACGGG1700.038.3823553
CTAGGGT1600.037.9687544
CTATGCG301.1395175E-437.5000041
GCGTTGA301.1395175E-437.5000049