##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732415
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63818736645208	31.0	31.0	34.0	30.0	34.0
2	31.845756845504255	33.0	31.0	34.0	30.0	34.0
3	31.976245707693042	33.0	31.0	34.0	30.0	34.0
4	35.65094516087191	37.0	35.0	37.0	33.0	37.0
5	35.40694415051576	37.0	35.0	37.0	33.0	37.0
6	35.421156038584684	37.0	35.0	37.0	33.0	37.0
7	35.472753834916	37.0	35.0	37.0	33.0	37.0
8	35.61958179447444	37.0	35.0	37.0	35.0	37.0
9	37.36404224380986	39.0	37.0	39.0	34.0	39.0
10	36.88754326440611	39.0	37.0	39.0	33.0	39.0
11	36.867625594778914	39.0	37.0	39.0	33.0	39.0
12	36.72861151123339	39.0	35.0	39.0	32.0	39.0
13	36.79133141729757	39.0	35.0	39.0	33.0	39.0
14	37.85458107766772	40.0	37.0	41.0	33.0	41.0
15	37.92410723428657	40.0	37.0	41.0	33.0	41.0
16	37.74375593072234	40.0	37.0	41.0	33.0	41.0
17	37.73048203545804	39.0	37.0	41.0	33.0	41.0
18	37.599883945577304	39.0	36.0	41.0	32.0	41.0
19	37.63718929841688	39.0	36.0	41.0	32.0	41.0
20	37.69968528771257	39.0	36.0	41.0	33.0	41.0
21	37.74993958343289	39.0	37.0	41.0	33.0	41.0
22	37.7081326843388	39.0	36.0	41.0	33.0	41.0
23	37.59651700197293	39.0	36.0	41.0	32.0	41.0
24	37.46197442706662	39.0	36.0	41.0	32.0	41.0
25	37.50759610330209	39.0	36.0	41.0	32.0	41.0
26	37.4297358737874	39.0	36.0	41.0	32.0	41.0
27	37.409891932852275	39.0	36.0	41.0	32.0	41.0
28	37.31342886205225	39.0	36.0	41.0	32.0	41.0
29	37.17324877289515	39.0	36.0	41.0	31.0	41.0
30	37.22285043315607	39.0	36.0	41.0	31.0	41.0
31	37.13360867814013	39.0	36.0	41.0	31.0	41.0
32	37.09092795751043	39.0	36.0	41.0	31.0	41.0
33	37.024574865342736	39.0	36.0	40.0	31.0	41.0
34	36.96955687690722	39.0	35.0	40.0	31.0	41.0
35	36.816565744830456	39.0	35.0	40.0	31.0	41.0
36	36.81877077886171	39.0	35.0	40.0	31.0	41.0
37	36.76889195333247	39.0	35.0	40.0	31.0	41.0
38	36.73136268372439	39.0	35.0	40.0	31.0	41.0
39	36.63387287262003	39.0	35.0	40.0	30.0	41.0
40	36.50282012247155	39.0	35.0	40.0	30.0	41.0
41	36.44005106394599	39.0	35.0	40.0	30.0	41.0
42	36.33101042441785	38.0	35.0	40.0	30.0	41.0
43	36.30227808005024	38.0	35.0	40.0	30.0	41.0
44	36.25334407405637	38.0	35.0	40.0	30.0	41.0
45	36.02743526552569	38.0	35.0	40.0	29.0	41.0
46	35.776010868155346	38.0	34.0	40.0	28.0	41.0
47	35.717272311462764	38.0	34.0	40.0	28.0	41.0
48	35.659508611920835	38.0	34.0	40.0	28.0	41.0
49	35.50839483079948	38.0	34.0	40.0	28.0	41.0
50	35.41394701091594	38.0	34.0	40.0	28.0	41.0
51	33.908872701951765	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	3.0
12	5.0
13	6.0
14	11.0
15	20.0
16	48.0
17	106.0
18	179.0
19	395.0
20	685.0
21	1220.0
22	1669.0
23	2355.0
24	2962.0
25	3674.0
26	4587.0
27	5932.0
28	7844.0
29	10283.0
30	13800.0
31	18454.0
32	24897.0
33	33872.0
34	48506.0
35	60115.0
36	72561.0
37	102003.0
38	151689.0
39	164491.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.18529795266345	36.710334987677754	19.636408320419434	24.467958739239364
2	19.28687970617751	35.005563785558735	20.379293160298463	25.32826334796529
3	19.819091635206814	27.059249196152457	27.509130752373995	25.612528416266734
4	24.069414198234607	27.252445676290083	20.182410245557506	28.495729879917807
5	17.136596055514975	35.292423011544	20.04136998832629	27.529610944614735
6	20.624509328727566	31.654594731129208	25.394755705440218	22.326140234703004
7	76.99733074827796	9.477823365168653	7.938122512509985	5.586723374043404
8	79.75560303926052	3.480130800161111	5.273512967375054	11.490753193203307
9	75.042564666207	6.301755152475032	10.998136302506092	7.657543878811876
10	34.18826758053836	25.865390523132376	16.92210017544698	23.024241720882287
11	23.51603940388987	25.018602841285336	29.32490459643781	22.14045315838698
12	22.32928053084658	20.98195695063591	38.7453834233324	17.943379095185108
13	24.679041253933903	25.095471829495576	27.905627274154682	22.319859642415842
14	18.451834001215158	26.570045670828694	33.81962412020508	21.15849620775107
15	17.839476253217097	31.403097970412947	27.016240792446904	23.74118498392305
16	18.483100428036018	29.423755657653107	29.944635213642538	22.148508700668337
17	16.82597980653045	30.068608644006474	30.157629212946212	22.947782336516866
18	17.923308506789184	29.348388550207194	29.034632005079086	23.693670937924537
19	18.19023367899347	32.46465460155786	32.29603435210912	17.049077367339553
20	19.254247933207267	33.07059522265383	28.59949618727088	19.07566065686803
21	17.970139879713003	30.075162305523506	29.122150693254508	22.83254712150898
22	16.90653522934402	32.41645788248466	29.63975341848542	21.0372534696859
23	19.868517165814463	28.441252568557445	26.46218332502748	25.228046940600617
24	15.802106729108498	34.02360683492282	27.34051050292525	22.833775933043423
25	17.815446160987964	29.340333007925835	28.092270092775273	24.751950738310928
26	20.103493238123196	32.01463651072138	26.038926018718893	21.84294423243653
27	16.001037663073532	29.37214557320645	30.823645064615008	23.803171699105015
28	20.47991917150796	31.219185844091125	26.658656635923624	21.642238348477296
29	19.285923963872943	34.835851259190484	26.181468156714434	19.696756620222143
30	20.419980475550066	29.188643050729436	27.386659202774382	23.004717270946117
31	23.70513984557935	33.110326795600855	23.05591775154796	20.128615607271833
32	22.014977847258727	29.095116839496733	25.49811240894848	23.39179290429606
33	21.67241249837865	29.590327887877777	26.52143934791068	22.215820265832896
34	21.62407924469051	30.583480676938624	26.18720261054184	21.60523746782903
35	18.3188492862653	33.51870182888116	27.73168217472335	20.430766710130186
36	21.348006253285366	31.130165275151384	29.096755254875994	18.42507321668726
37	22.71458121420233	29.755671306567997	26.984291692551356	20.545455786678318
38	24.836192595727834	28.518531160612493	26.360328502283544	20.284947741376133
39	24.067366179010534	26.562126663162278	27.43867889106586	21.93182826676133
40	21.689752394475807	30.469883877309993	24.391772424103824	23.448591304110376
41	20.754490282148783	28.342674576572023	30.100557743902023	20.80227739737717
42	21.37940921472116	31.82335151519289	25.60064990476711	21.196589365318843
43	19.535645774595004	27.87053787811555	29.714164783626767	22.87965156366268
44	21.493142548964727	24.850528730296347	28.335165172750422	25.3211635479885
45	24.93081108388004	27.759944840015567	26.820859758470267	20.48838431763413
46	17.95402879515029	26.18105855286962	34.471576906535226	21.393335745444865
47	20.72731989377607	28.759105152133696	26.4258651174539	24.087709836636336
48	20.03659127680345	25.439265989910094	33.303523275738485	21.220619457547976
49	21.454230183707324	25.28252425196098	28.827099390372947	24.436146173958754
50	20.019387915321232	24.538547135162442	27.12546848439751	28.31659646511882
51	19.191032406490855	23.49965525009728	33.37165404859267	23.937658294819194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1443.0
1	1158.0
2	873.0
3	617.0
4	361.0
5	430.0
6	499.0
7	526.0
8	553.0
9	628.0
10	703.0
11	844.0
12	985.0
13	1118.0
14	1251.0
15	1346.5
16	1442.0
17	1834.0
18	2226.0
19	2479.0
20	2732.0
21	3447.0
22	4162.0
23	5212.0
24	6262.0
25	7844.0
26	10712.5
27	11999.0
28	14374.5
29	16750.0
30	18416.0
31	20082.0
32	21939.0
33	23796.0
34	25960.5
35	28125.0
36	29918.5
37	31712.0
38	32709.5
39	33707.0
40	34095.0
41	34483.0
42	35972.0
43	37461.0
44	55955.0
45	74449.0
46	75791.5
47	77134.0
48	75192.0
49	73250.0
50	66060.5
51	58871.0
52	51508.5
53	44146.0
54	37909.0
55	31672.0
56	27949.5
57	24227.0
58	21869.0
59	19511.0
60	18013.5
61	16516.0
62	15003.5
63	13491.0
64	11102.0
65	8713.0
66	7337.5
67	5962.0
68	5018.0
69	4074.0
70	3350.0
71	2626.0
72	2393.5
73	2161.0
74	1873.0
75	1417.5
76	1250.0
77	1023.0
78	796.0
79	593.5
80	391.0
81	380.5
82	370.0
83	230.5
84	91.0
85	75.5
86	60.0
87	40.5
88	21.0
89	15.5
90	10.0
91	5.5
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	732415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.57484645433648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91790012594677	25.153569934099785
2	9.940134949901566	6.674770093566575
3	3.6899364833998805	3.716671525692159
4	1.9769792660717858	2.6550710120706813
5	1.3063537911238456	2.1930313976011737
6	0.9620535211736623	1.938047955255568
7	0.7541802126146955	1.772503938620607
8	0.6190426110443837	1.6627408491605382
9	0.5015418904479729	1.5155272765987506
>10	4.955819436742572	34.90869865648224
>50	0.3244981124300177	6.788132282948406
>100	0.04337553575322062	2.7045090627972423
>500	0.0036828285073489202	0.7777610263904083
>1k	0.0028644221723824934	1.8275529364149816
>5k	4.092031674832134E-4	0.8654154222280839
>10k+	0.0012276095024496402	4.8459966300728645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	12689	1.7324877289514824	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGT	12196	1.665176163786924	No Hit
AATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	10387	1.4181850453636258	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	6299	0.8600315394960508	Illumina Single End Adapter 1 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	2889	0.3944485025566107	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	2184	0.29819159902514286	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT	2046	0.27934982216366405	No Hit
AACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	2009	0.2742980414109487	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGATC	1800	0.24576230688885398	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1201	0.1639780725408409	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1173	0.16015510332256985	No Hit
AATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	895	0.12219848036973573	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9957469467446735	0.0
2	0.0	0.0	0.0	1.39128772622079	0.0
3	0.0	0.0	0.0	2.137722466088215	0.0
4	0.0	0.0	0.0	5.436535297611327	0.0
5	0.0	0.0	0.0	5.8308472655529995	0.0
6	0.0	0.0	0.0	7.76581582845791	0.0
7	0.0	0.0	0.0	9.108224162530806	0.0
8	0.0	0.0	0.0	9.998702921158086	0.0
9	0.0	0.0	0.0	13.037553845838767	0.0
10	0.0	0.0	0.0	15.102503362164892	0.0
11	0.0	0.0	0.0	18.82825993459992	0.0
12	0.0	0.0	0.0	19.77649283534608	0.0
13	0.0	0.0	0.0	20.23743369537762	0.0
14	0.0	0.0	0.0	20.895667073994936	0.0
15	0.0	0.0	0.0	21.27482369967846	0.0
16	0.0	0.0	0.0	22.001460920379838	0.0
17	0.0	0.0	0.0	22.972768171050564	0.0
18	0.0	0.0	0.0	24.342483428111112	0.0
19	0.0	0.0	0.0	24.9417338530751	0.0
20	0.0	0.0	0.0	25.481182116696136	0.0
21	0.0	0.0	0.0	26.112381641555675	0.0
22	0.0	0.0	0.0	26.77198036632237	0.0
23	0.0	0.0	0.0	27.42447929111228	0.0
24	0.0	0.0	0.0	27.957646962446155	0.0
25	0.0	0.0	0.0	28.418724357092632	0.0
26	0.0	0.0	0.0	28.831058894206155	0.0
27	0.0	0.0	0.0	29.28831331963436	0.0
28	1.3653461493825223E-4	0.0	0.0	29.70556310288566	0.0
29	1.3653461493825223E-4	0.0	0.0	30.152577432193496	0.0
30	1.3653461493825223E-4	0.0	0.0	30.748960630243783	0.0
31	1.3653461493825223E-4	0.0	0.0	31.17249100578224	0.0
32	1.3653461493825223E-4	0.0	0.0	31.60052702361366	0.0
33	1.3653461493825223E-4	0.0	0.0	32.00166572230225	0.0
34	1.3653461493825223E-4	0.0	0.0	32.38915095949701	0.0
35	1.3653461493825223E-4	0.0	0.0	32.78878777742127	0.0
36	1.3653461493825223E-4	0.0	0.0	33.1975724145464	0.0
37	2.7306922987650447E-4	0.0	0.0	33.59639002478103	0.0
38	2.7306922987650447E-4	0.0	0.0	34.015824361871346	0.0
39	2.7306922987650447E-4	0.0	0.0	34.438125925875354	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	30	2.1644191E-6	45.000004	1
CACTACG	35	1.2111195E-7	45.0	1
GCACTAG	35	1.2111195E-7	45.0	1
TACGGGT	80	0.0	42.187504	4
ACGGGTA	125	0.0	41.399998	5
ACACTAG	60	3.6379788E-12	41.250004	1
GCGGGTT	150	0.0	40.5	5
CGGGCTA	140	0.0	40.17857	6
TACGGCT	1610	0.0	40.108696	6
AACACGG	230	0.0	40.108692	2
CACGCAG	45	1.9266736E-8	40.0	1
TATGCGG	40	3.4559343E-7	39.375004	2
TGTTCGG	40	3.4559343E-7	39.375004	2
GTACGCG	40	3.4559343E-7	39.375004	1
CGACCGG	35	6.2451854E-6	38.571426	2
CCCGACG	35	6.2451854E-6	38.571426	1
GTACGGG	170	0.0	38.382355	3
CTAGGGT	160	0.0	37.968754	4
CTATGCG	30	1.1395175E-4	37.500004	1
GCGTTGA	30	1.1395175E-4	37.500004	9
>>END_MODULE