FastQCFastQC Report
Sat 18 Jun 2016
SRR3553105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887536
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC191682.159687043680482No Hit
AATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT185162.086225234807377No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGT180102.0292134628905196No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT76030.8566413080708839Illumina Single End Adapter 1 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT46410.5229083665338645No Hit
AACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG42370.47738908618917997No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT41420.46668529501901895No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGATC28440.3204377061888194No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTAT28380.31976167727280924No Hit
AATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC19730.2223008418813434No Hit
ACCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCG14850.16731715671251646No Hit
ACCCTGGGAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC11920.13430441131401993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9310.10489715346757766No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC9200.10365776712155901No Hit
ACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG8950.10084097997151666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG750.042.01
ACCCTGG11950.039.916321
CGAACAG850.039.7058831
ACAGGGA10250.039.512194
TACCCGG1200.039.3749962
CATAGCG750.039.01
CCACGGG7000.038.892863
ATGGGCG1750.038.571435
AACGTAG356.2466825E-638.5714261
AAGTACG356.2466825E-638.5714261
CTGCGTA356.2466825E-638.57142613
TAACACG356.2466825E-638.5714261
GCTACGA700.038.5714269
CATACCG659.094947E-1238.0769271
TACGGCT25150.038.021876
CTAGGGT2200.037.8409084
AACGGGC3650.037.602744
ACGTACG301.1397125E-437.4999961
CGATCGC301.1397125E-437.49999633
TACGTGC301.1397125E-437.49999625
Produced by FastQC (version 0.11.5)