##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	887536
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.632630113032036	31.0	31.0	34.0	30.0	34.0
2	31.847359431054063	33.0	31.0	34.0	30.0	34.0
3	31.948315335941302	33.0	31.0	34.0	30.0	34.0
4	35.61223094049143	37.0	35.0	37.0	33.0	37.0
5	35.344879531647166	37.0	35.0	37.0	33.0	37.0
6	35.369562474085555	37.0	35.0	37.0	33.0	37.0
7	35.352377819040576	37.0	35.0	37.0	33.0	37.0
8	35.52030002163293	37.0	35.0	37.0	33.0	37.0
9	37.22885832236664	39.0	37.0	39.0	34.0	39.0
10	36.78310062915758	39.0	37.0	39.0	32.0	39.0
11	36.80861621387752	39.0	37.0	39.0	32.0	39.0
12	36.62373244578248	39.0	35.0	39.0	32.0	39.0
13	36.7273012024301	39.0	35.0	39.0	32.0	39.0
14	37.797643137855815	40.0	37.0	41.0	33.0	41.0
15	37.88147748373024	40.0	37.0	41.0	33.0	41.0
16	37.637231616520346	40.0	36.0	41.0	32.0	41.0
17	37.62469240684322	39.0	36.0	41.0	32.0	41.0
18	37.47206761190532	39.0	36.0	41.0	32.0	41.0
19	37.47800539921761	39.0	36.0	41.0	32.0	41.0
20	37.56813695444467	39.0	36.0	41.0	32.0	41.0
21	37.649483513908166	39.0	36.0	41.0	32.0	41.0
22	37.610642272538804	39.0	36.0	41.0	32.0	41.0
23	37.49553595572461	39.0	36.0	41.0	32.0	41.0
24	37.360428196715404	39.0	36.0	41.0	32.0	41.0
25	37.431890086711974	39.0	36.0	41.0	32.0	41.0
26	37.32439810892178	39.0	36.0	41.0	32.0	41.0
27	37.1792547006544	39.0	36.0	41.0	31.0	41.0
28	37.14544874799445	39.0	36.0	41.0	31.0	41.0
29	36.977649357321845	39.0	36.0	41.0	31.0	41.0
30	37.03905982405221	39.0	36.0	41.0	31.0	41.0
31	36.972957716644736	39.0	36.0	41.0	31.0	41.0
32	36.87445917686719	39.0	35.0	41.0	31.0	41.0
33	36.89957365109697	39.0	35.0	41.0	31.0	41.0
34	36.74388081159525	39.0	35.0	41.0	30.0	41.0
35	36.714251590921386	39.0	35.0	40.0	30.0	41.0
36	36.60640807809486	39.0	35.0	40.0	30.0	41.0
37	36.55780948603775	39.0	35.0	40.0	30.0	41.0
38	36.57350913089723	39.0	35.0	40.0	30.0	41.0
39	36.47862621910548	39.0	35.0	40.0	30.0	41.0
40	36.31006629590237	39.0	35.0	40.0	30.0	41.0
41	36.33926736492942	39.0	35.0	40.0	30.0	41.0
42	36.284947314813145	38.0	35.0	40.0	30.0	41.0
43	36.24557539074471	38.0	35.0	40.0	30.0	41.0
44	36.214604252672565	38.0	35.0	40.0	30.0	41.0
45	36.00649100430856	38.0	35.0	40.0	30.0	41.0
46	35.75713323177877	38.0	35.0	40.0	29.0	41.0
47	35.708408447657334	38.0	35.0	40.0	29.0	41.0
48	35.635502109210215	38.0	34.0	40.0	28.0	41.0
49	35.48161426691424	38.0	34.0	40.0	28.0	41.0
50	35.43284441419841	38.0	34.0	40.0	28.0	41.0
51	33.897076850967174	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	5.0
11	7.0
12	10.0
13	16.0
14	21.0
15	41.0
16	53.0
17	135.0
18	271.0
19	565.0
20	1169.0
21	1986.0
22	2946.0
23	3676.0
24	4514.0
25	5015.0
26	6019.0
27	7480.0
28	9784.0
29	12931.0
30	17462.0
31	23249.0
32	31711.0
33	41437.0
34	58469.0
35	72421.0
36	86648.0
37	118505.0
38	176212.0
39	204716.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.771222801103278	39.12292008436841	18.795406608858684	24.31045050566963
2	20.062735483405746	35.34335508644156	19.543545275909935	25.05036415424276
3	18.555529015161078	25.47074146851508	29.567476699536694	26.406252816787152
4	25.745772565845215	26.40568945935714	19.374312703935388	28.474225270862252
5	16.433812262263164	36.302414775287986	20.219010834490096	27.044762127958755
6	21.215928142633086	31.078964684249428	26.150037857619296	21.555069315498187
7	75.61462295613924	10.683510302680679	7.928917812911251	5.772948928268825
8	77.95526040633844	3.1543509221034416	5.502988047808765	13.387400623749347
9	73.7968938724739	7.30652052423789	11.044735086802113	7.851850516486092
10	34.20334499107642	27.65780768329397	16.758418813434048	21.38042851219556
11	23.13438553478394	23.51656721530169	30.441920102395848	22.907127147518523
12	22.868706170791945	20.663049160822773	38.649812514647294	17.81843215373799
13	24.05840439148384	26.57785148996773	27.415676659876333	21.9480674586721
14	18.510911106704402	25.40246254799805	34.80861621387752	21.27801013142002
15	17.008324169385805	31.5093697607759	26.094378143534456	25.38792792630383
16	18.421111932361054	28.89347587027456	29.529506408754123	23.155905788610266
17	18.019550756251014	28.579460438787834	29.287600728308487	24.113388076652665
18	18.558796488255126	28.378792522218816	28.396932631465088	24.66547835806097
19	18.114758161922445	31.435682608930794	31.377994808097924	19.071564421048837
20	19.794915361179715	32.61095887941447	28.247192226568842	19.346933532836978
21	18.46133565286366	28.923671828523013	29.231828342737646	23.38316417587568
22	17.847276054154424	31.353770438607558	29.117466784445927	21.68148672279209
23	19.6609489643237	28.649091417136884	25.423982801824373	26.265976816715042
24	16.193709325593552	35.266287790016406	26.303496421553607	22.236506462836438
25	17.979552378720413	27.381199185159815	28.02793351480954	26.611314921310235
26	21.091426150601215	31.3631221719457	24.92552414775288	22.619927529700202
27	16.34637918912585	29.587870238503	29.248278379693893	24.817472192677254
28	21.31507905031458	27.288808566638423	25.776419210037677	25.619693173009324
29	17.694493519136127	34.896950658902846	25.992185105730925	21.4163707162301
30	21.010978709596003	29.823128301274537	25.071997079555082	24.09389590957437
31	25.036730904436556	30.851818968470013	22.28923671828523	21.822213408808206
32	18.717212597573507	29.118593499305945	23.54112959925006	28.623064303870493
33	21.88226731084711	26.43960357664365	27.62085143588542	24.057277676623823
34	20.605361359989903	28.948572226929386	24.843386634457644	25.602679778623067
35	18.260217050350633	32.10900740927692	25.84165600043266	23.789119539939787
36	21.907956409655498	30.183226370535955	28.292824178399524	19.615993041409023
37	19.79514070415172	32.45659894359215	23.183960988624687	24.56429936363145
38	24.273494258261074	27.55245984388239	28.007652647329234	20.166393250527303
39	21.202970921742892	25.895625642227472	28.09249427628851	24.808909159741127
40	20.31173946746949	30.909281426330875	27.25264101963188	21.526338086567755
41	21.348091795713074	26.054943123433866	30.926069477745127	21.67089560310793
42	21.973193200050478	30.118778280542983	24.62829676768041	23.279731751726125
43	20.24188314614844	26.979412666077767	28.78193109913288	23.99677308864091
44	20.648627210614556	24.85442844008581	26.832376376845556	27.664567972454073
45	25.966608678408537	26.094265472048455	26.716437417749816	21.222688431793188
46	17.154233743758	24.884737069820268	35.12015287267221	22.84087631374953
47	20.45686034143967	28.338568801716214	26.649510555064808	24.555060301779307
48	19.814745542716015	25.166641127796506	33.29949433037083	21.719118999116656
49	21.46358006886481	25.431644462872494	28.842773701573794	24.2620017666889
50	19.4945331794992	24.42210794829731	27.054902561698906	29.028456310504584
51	18.567472192677254	23.368516882695463	33.06040543707523	25.003605487552054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1253.0
1	1009.5
2	766.0
3	561.5
4	357.0
5	407.5
6	458.0
7	510.5
8	563.0
9	633.0
10	703.0
11	822.0
12	941.0
13	1072.0
14	1203.0
15	1325.0
16	1447.0
17	1607.0
18	1767.0
19	2322.0
20	2877.0
21	3402.0
22	3927.0
23	5327.0
24	6727.0
25	8435.0
26	11766.0
27	13389.0
28	15033.0
29	16677.0
30	19184.5
31	21692.0
32	24876.0
33	28060.0
34	30139.5
35	32219.0
36	34008.0
37	35797.0
38	37114.0
39	38431.0
40	38198.5
41	37966.0
42	41008.0
43	44050.0
44	72363.5
45	100677.0
46	97086.0
47	93495.0
48	90617.5
49	87740.0
50	78422.0
51	69104.0
52	60986.5
53	52869.0
54	46748.5
55	40628.0
56	36370.5
57	32113.0
58	29177.0
59	26241.0
60	24351.0
61	22461.0
62	19824.0
63	17187.0
64	15129.5
65	13072.0
66	11391.0
67	9710.0
68	8167.0
69	6624.0
70	5378.5
71	4133.0
72	3757.5
73	3382.0
74	3149.5
75	2357.0
76	1797.0
77	1492.0
78	1187.0
79	794.0
80	401.0
81	310.5
82	220.0
83	151.5
84	83.0
85	55.5
86	28.0
87	24.0
88	20.0
89	16.5
90	13.0
91	13.5
92	14.0
93	9.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	887536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.178746734577466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5443638188916	22.042898590962153
2	9.693358629126214	5.656801128934096
3	3.6419927067050564	3.188063483943753
4	1.9313715481272582	2.254200050126962
5	1.2353308838692545	1.8022703496911354
6	0.9063158678077672	1.5867096700974976
7	0.7042523441537902	1.4384440551107154
8	0.5225708079543399	1.2198369004946563
9	0.4769572486435746	1.2525313285252577
>10	4.466422200775269	28.346582850441155
>50	0.7398521629652112	14.741459413205632
>100	0.12707681988553693	5.505839417513556
>500	0.005457286743550666	1.1088567455396876
>1k	0.003118449567743237	2.6560696196371714
>5k	3.8980619596790464E-4	0.8647694980344902
>10k+	0.001169418587903714	6.334666897742062
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	19168	2.159687043680482	No Hit
AATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	18516	2.086225234807377	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGT	18010	2.0292134628905196	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	7603	0.8566413080708839	Illumina Single End Adapter 1 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	4641	0.5229083665338645	No Hit
AACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	4237	0.47738908618917997	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	4142	0.46668529501901895	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGATC	2844	0.3204377061888194	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTAT	2838	0.31976167727280924	No Hit
AATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	1973	0.2223008418813434	No Hit
ACCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCG	1485	0.16731715671251646	No Hit
ACCCTGGGAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	1192	0.13430441131401993	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	931	0.10489715346757766	No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	920	0.10365776712155901	No Hit
ACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	895	0.10084097997151666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2534297200338917E-4	0.0	0.0	1.0425492599736799	0.0
2	2.2534297200338917E-4	0.0	0.0	1.3474383010942654	0.0
3	2.2534297200338917E-4	0.0	0.0	2.255119792323917	0.0
4	2.2534297200338917E-4	0.0	0.0	5.898014277730706	0.0
5	2.2534297200338917E-4	0.0	0.0	6.376079392835896	0.0
6	2.2534297200338917E-4	0.0	0.0	8.067278397721331	0.0
7	2.2534297200338917E-4	0.0	0.0	9.362099114852805	0.0
8	2.2534297200338917E-4	0.0	0.0	10.170629698400965	0.0
9	2.2534297200338917E-4	0.0	0.0	13.360810153052947	0.0
10	3.3801445800508376E-4	0.0	0.0	14.997138144255556	0.0
11	3.3801445800508376E-4	0.0	0.0	18.896022245858195	0.0
12	3.3801445800508376E-4	0.0	0.0	19.73993167601089	0.0
13	3.3801445800508376E-4	0.0	0.0	20.16887202321934	0.0
14	3.3801445800508376E-4	0.0	0.0	20.810310793026986	0.0
15	3.3801445800508376E-4	0.0	0.0	21.193393845432748	0.0
16	3.3801445800508376E-4	0.0	0.0	21.773313983883472	0.0
17	3.3801445800508376E-4	0.0	0.0	22.740598691208017	0.0
18	3.3801445800508376E-4	0.0	0.0	24.022236844477295	0.0
19	3.3801445800508376E-4	0.0	0.0	24.578721313839665	0.0
20	3.3801445800508376E-4	0.0	0.0	25.02332299760235	0.0
21	3.3801445800508376E-4	0.0	0.0	25.547583421968234	0.0
22	3.3801445800508376E-4	0.0	0.0	26.062830127453985	0.0
23	3.3801445800508376E-4	0.0	0.0	26.56996448594761	0.0
24	3.3801445800508376E-4	0.0	0.0	26.961835914261506	0.0
25	3.3801445800508376E-4	0.0	0.0	27.303568531304645	0.0
26	3.3801445800508376E-4	0.0	0.0	27.656793639919957	0.0
27	3.3801445800508376E-4	0.0	0.0	28.027032142921527	0.0
28	3.3801445800508376E-4	0.0	0.0	28.35851165473851	0.0
29	3.3801445800508376E-4	0.0	0.0	28.76604441960664	0.0
30	4.5068594400677833E-4	0.0	0.0	29.25796812749004	0.0
31	4.5068594400677833E-4	0.0	0.0	29.587982909989	0.0
32	4.5068594400677833E-4	0.0	0.0	29.913378161561898	0.0
33	4.5068594400677833E-4	0.0	0.0	30.22942167979665	0.0
34	4.5068594400677833E-4	0.0	0.0	30.549746714499467	0.0
35	4.5068594400677833E-4	0.0	0.0	30.878634782138416	0.0
36	4.5068594400677833E-4	0.0	0.0	31.194565628887165	0.0
37	4.5068594400677833E-4	0.0	0.0	31.515566692505995	0.0
38	4.5068594400677833E-4	0.0	0.0	31.83577905572281	0.0
39	4.5068594400677833E-4	0.0	0.0	32.17221611292387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAG	75	0.0	42.0	1
ACCCTGG	1195	0.0	39.91632	1
CGAACAG	85	0.0	39.705883	1
ACAGGGA	1025	0.0	39.51219	4
TACCCGG	120	0.0	39.374996	2
CATAGCG	75	0.0	39.0	1
CCACGGG	700	0.0	38.89286	3
ATGGGCG	175	0.0	38.57143	5
AACGTAG	35	6.2466825E-6	38.571426	1
AAGTACG	35	6.2466825E-6	38.571426	1
CTGCGTA	35	6.2466825E-6	38.571426	13
TAACACG	35	6.2466825E-6	38.571426	1
GCTACGA	70	0.0	38.571426	9
CATACCG	65	9.094947E-12	38.076927	1
TACGGCT	2515	0.0	38.02187	6
CTAGGGT	220	0.0	37.840908	4
AACGGGC	365	0.0	37.60274	4
ACGTACG	30	1.1397125E-4	37.499996	1
CGATCGC	30	1.1397125E-4	37.499996	33
TACGTGC	30	1.1397125E-4	37.499996	25
>>END_MODULE