FastQCFastQC Report
Sat 18 Jun 2016
SRR3553104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792840
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT59520.750718934463448TruSeq Adapter, Index 22 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT28970.3653952878260431No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT24470.30863730386963323No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC24350.30712375763079564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12530.15803945310529235No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC11530.14542656778164573TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG253.889707E-545.01
CCTAGCG253.889707E-545.01
ACTAACG406.8102963E-945.01
CGTCGGA406.8102963E-945.03
TCTAGCG1000.042.751
TAGCCCG451.9270374E-840.01
TGTCCGC451.9270374E-840.021
CGCACGG1650.039.5454522
ACGGGAC4200.039.1071435
ACGCGAG356.245835E-638.571431
GTACCGG700.038.571432
ACCTAGC356.245835E-638.5714340
TGCGACG356.245835E-638.571431
CGTACGA356.245835E-638.5714338
TAGGGTG1700.038.382355
CGAGATA3250.038.0769219
TAGCAGG2550.037.9411772
CTCACGG2500.037.8000032
CCAACGG1550.037.7419362
CACTGCG301.1396023E-437.5000041
Produced by FastQC (version 0.11.5)