##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792840
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66396372534181	31.0	31.0	34.0	30.0	34.0
2	31.86805660662933	33.0	31.0	34.0	30.0	34.0
3	32.04046970384945	33.0	31.0	34.0	30.0	34.0
4	35.71697820493416	37.0	35.0	37.0	33.0	37.0
5	35.47893648150951	37.0	35.0	37.0	33.0	37.0
6	34.791075122344985	37.0	35.0	37.0	32.0	37.0
7	35.29840068614096	37.0	35.0	37.0	33.0	37.0
8	35.74453862065486	37.0	35.0	37.0	35.0	37.0
9	37.41112582614399	39.0	37.0	39.0	35.0	39.0
10	36.90019045456839	39.0	37.0	39.0	33.0	39.0
11	36.9218707431512	39.0	37.0	39.0	33.0	39.0
12	36.8853816659099	39.0	37.0	39.0	33.0	39.0
13	36.88505246960295	39.0	37.0	39.0	33.0	39.0
14	38.05993895363503	40.0	37.0	41.0	33.0	41.0
15	38.060120579183696	40.0	37.0	41.0	33.0	41.0
16	37.92256319055547	40.0	37.0	41.0	33.0	41.0
17	37.93187528378992	40.0	37.0	41.0	33.0	41.0
18	37.911679531809696	40.0	37.0	41.0	33.0	41.0
19	37.95807603047273	40.0	37.0	41.0	33.0	41.0
20	37.88040209878412	40.0	37.0	41.0	33.0	41.0
21	37.88434110287069	40.0	37.0	41.0	33.0	41.0
22	37.863550022703194	40.0	37.0	41.0	33.0	41.0
23	37.78813127491045	40.0	37.0	41.0	33.0	41.0
24	37.65060667978407	39.0	37.0	41.0	32.0	41.0
25	37.67353438272539	39.0	37.0	41.0	32.0	41.0
26	37.66655945714142	39.0	37.0	41.0	33.0	41.0
27	37.57362015034559	40.0	37.0	41.0	32.0	41.0
28	37.50272690580697	39.0	36.0	41.0	32.0	41.0
29	37.310104182432774	39.0	36.0	41.0	32.0	41.0
30	37.332474395842794	39.0	36.0	41.0	32.0	41.0
31	37.34671812723879	39.0	36.0	41.0	32.0	41.0
32	37.2983905958327	39.0	36.0	41.0	32.0	41.0
33	37.12742671913627	39.0	36.0	41.0	31.0	41.0
34	37.16256495635942	39.0	36.0	41.0	31.0	41.0
35	37.11869860249231	39.0	36.0	41.0	31.0	41.0
36	37.08144770697745	39.0	36.0	41.0	31.0	41.0
37	37.02494198072751	39.0	36.0	41.0	31.0	41.0
38	36.878522778870895	39.0	35.0	41.0	31.0	41.0
39	36.859874627919886	39.0	35.0	40.0	31.0	41.0
40	36.781385903839364	39.0	35.0	40.0	31.0	41.0
41	36.76079536854851	39.0	35.0	40.0	31.0	41.0
42	36.67901089753292	39.0	35.0	40.0	31.0	41.0
43	36.573755108218556	39.0	35.0	40.0	30.0	41.0
44	36.50127137884062	39.0	35.0	40.0	30.0	41.0
45	36.30949245749458	38.0	35.0	40.0	30.0	41.0
46	36.137547298319966	38.0	35.0	40.0	30.0	41.0
47	36.0483514958882	38.0	35.0	40.0	30.0	41.0
48	35.9616366479996	38.0	35.0	40.0	29.0	41.0
49	35.83243277332122	38.0	35.0	40.0	29.0	41.0
50	35.736811967105595	38.0	34.0	40.0	28.0	41.0
51	34.56138060642753	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	3.0
13	9.0
14	14.0
15	38.0
16	62.0
17	113.0
18	227.0
19	412.0
20	746.0
21	1156.0
22	1740.0
23	2255.0
24	2839.0
25	3716.0
26	4670.0
27	6007.0
28	7940.0
29	10501.0
30	13988.0
31	18328.0
32	24102.0
33	32540.0
34	48090.0
35	61044.0
36	76691.0
37	111726.0
38	169089.0
39	194741.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.206649513142626	32.55612733969023	21.849048988446597	26.388174158720552
2	21.171863175420008	29.938701377327074	22.146839210937895	26.74259623631502
3	20.672140658897128	29.561828363856517	22.989127692851017	26.77690328439534
4	19.885096614701578	30.305862469098432	20.936885121840472	28.872155794359518
5	17.854926593007416	34.12693607789718	20.029766409363805	27.988370919731597
6	19.44137530901569	35.73230412189092	21.53006911861157	23.29625145048181
7	83.73833308107562	5.191589728066193	5.889208415317087	5.180868775541093
8	86.47696887139902	3.239997981938348	4.374148630240654	5.908884516421977
9	78.34203622420665	6.922708238736694	9.062862620453055	5.672392916603602
10	32.505423540689165	30.046919933403966	15.275213157761968	22.172443368144897
11	22.067504162252156	26.47192371726956	30.849099439987892	20.61147268049039
12	21.257000151354625	21.276171737046568	37.55070379900106	19.91612431259775
13	23.936103122950406	24.869835023459967	29.623883759648855	21.57017809394077
14	19.14648605014883	28.414686443670856	30.694717723626457	21.74410978255386
15	18.537157560163465	31.992331365723224	28.351369759346145	21.119141314767166
16	18.606528429443518	30.622319761868727	28.596690378891076	22.17446142979668
17	16.845895767115685	30.222112910549413	30.403612330356694	22.528378991978204
18	18.341531708793703	29.54908934967963	29.973008425407393	22.136370516119268
19	19.016068815902326	32.83562887846224	29.834518944553757	18.313783361081683
20	19.416654053781343	30.348620150345596	29.917259472276882	20.317466323596186
21	18.906841228999546	31.345416477473385	28.922859593360577	20.824882700166487
22	16.923465011856113	30.969930881388425	29.41678018263458	22.689823924120883
23	18.003506382119973	30.385575904343874	27.443620402603297	24.16729731093285
24	16.09618586347813	31.293577518793196	29.386130871298118	23.224105746430553
25	17.111775389738156	31.051157862872707	28.739215983048283	23.097850764340848
26	19.40643761666919	30.71060995913425	28.054462438827503	21.828489985369053
27	16.584178396650017	30.11704757580344	28.929670551435343	24.369103476111196
28	18.40913677412845	31.589854195045657	28.68901669946017	21.311992331365722
29	17.755789314363554	31.55277231219414	26.64434185964381	24.047096513798497
30	18.19951062004944	33.194969981332925	27.032818727612128	21.5727006710055
31	17.586272135613743	31.7926693910499	25.803314666263056	24.817743807073306
32	18.77390141768831	31.052545280258315	26.672973109328492	23.500580192724886
33	17.091594773220322	29.308435497704455	30.107335654104233	23.49263407497099
34	16.384642550829927	29.650370818828513	29.073205186418445	24.89178144392311
35	18.342919126179304	29.55766611169971	28.33699107007719	23.76242369204379
36	16.69264921043338	29.384995711618988	31.046364966449723	22.875990111497906
37	17.572397961757733	31.079915241410628	27.208011704757578	24.13967509207406
38	18.63339387518289	29.026663639574192	29.062484233893343	23.27745825134958
39	18.348342666868472	27.809015690429344	30.413828767468846	23.428812875233337
40	19.730841027193378	28.747666616215124	29.823797992028656	21.697694364562835
41	18.396902275364514	27.223525553705663	31.921068563644617	22.458503607285202
42	21.378083850461632	28.844281317794255	27.812673427173202	21.96496140457091
43	17.63987689823924	28.387064224812068	30.187931991322337	23.785126885626358
44	19.411987286211595	27.179002068513192	27.679985873568434	25.729024771706776
45	20.19095908380001	27.513874173856014	29.447681751677514	22.847484990666462
46	16.391453508904696	28.11386912870188	32.715428081327886	22.779249281065535
47	18.337621714343374	28.225493163816157	29.575576408859288	23.861308712981184
48	19.245749457645932	26.52060945461884	31.68911760254276	22.54452348519247
49	19.692876242369202	26.369002573028606	29.58844155188941	24.34967963271278
50	18.29486403309621	25.803819181676	28.62620453054841	27.275112254679378
51	18.975076938600473	24.85116795318097	31.599187730185157	24.5745673780334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1518.0
1	1182.0
2	846.0
3	618.0
4	390.0
5	386.5
6	383.0
7	435.5
8	488.0
9	575.5
10	663.0
11	819.5
12	976.0
13	1134.0
14	1292.0
15	1594.0
16	1896.0
17	2052.5
18	2209.0
19	2686.0
20	3163.0
21	4251.0
22	5339.0
23	6263.5
24	7188.0
25	9022.5
26	12746.5
27	14636.0
28	18041.0
29	21446.0
30	24599.0
31	27752.0
32	30491.0
33	33230.0
34	33772.0
35	34314.0
36	37571.5
37	40829.0
38	40784.5
39	40740.0
40	40758.5
41	40777.0
42	42027.5
43	43278.0
44	51889.5
45	60501.0
46	64122.0
47	67743.0
48	72526.5
49	77310.0
50	70423.0
51	63536.0
52	55044.5
53	46553.0
54	40133.5
55	33714.0
56	29512.0
57	25310.0
58	22467.5
59	19625.0
60	19041.5
61	18458.0
62	15717.5
63	12977.0
64	10662.5
65	8348.0
66	7784.5
67	7221.0
68	6350.0
69	5479.0
70	4665.0
71	3851.0
72	3278.5
73	2706.0
74	2474.5
75	1748.0
76	1253.0
77	1054.5
78	856.0
79	726.5
80	597.0
81	395.0
82	193.0
83	135.5
84	78.0
85	58.0
86	38.0
87	30.0
88	22.0
89	17.5
90	13.0
91	8.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	792840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.964267762160524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.96075921351817	24.71026538369255
2	10.162723522605758	6.700134787839669
3	3.7391139659143366	3.6977146189690253
4	2.014387062673815	2.6561117804244674
5	1.256220866862644	2.0705200511836805
6	0.9364491223427144	1.8521615768727278
7	0.712036760923695	1.643023931051311
8	0.5749236276380637	1.5161549123403049
9	0.48144214554213816	1.428334901787609
>10	4.399644921124725	30.807966521151165
>50	0.6475441345737439	14.636103159461353
>100	0.10772886099773968	5.244972459880037
>500	0.0046838635216408546	0.9602401498401705
>1k	0.0019516098006836893	1.3104711143135652
>5k	3.903219601367379E-4	0.7658246511923751
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	5952	0.750718934463448	TruSeq Adapter, Index 22 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	2897	0.3653952878260431	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT	2447	0.30863730386963323	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	2435	0.30712375763079564	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1253	0.15803945310529235	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1153	0.14542656778164573	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2612885323646638E-4	0.0	0.0	0.836486554664245	0.0
2	1.2612885323646638E-4	0.0	0.0	1.1897734725795872	0.0
3	1.2612885323646638E-4	0.0	0.0	1.6806669693759144	0.0
4	1.2612885323646638E-4	0.0	0.0	3.595555219211947	0.0
5	1.2612885323646638E-4	0.0	0.0	3.89990414207154	0.0
6	1.2612885323646638E-4	0.0	0.0	5.3954139548963225	0.0
7	1.2612885323646638E-4	0.0	0.0	6.535871045860451	0.0
8	1.2612885323646638E-4	0.0	0.0	7.592956964835276	0.0
9	1.2612885323646638E-4	0.0	0.0	9.170324403410524	0.0
10	1.2612885323646638E-4	0.0	0.0	11.347308410271934	0.0
11	1.2612885323646638E-4	0.0	0.0	13.411028706926997	0.0
12	1.2612885323646638E-4	0.0	0.0	14.284723273295999	0.0
13	1.2612885323646638E-4	0.0	0.0	14.685434640028253	0.0
14	1.2612885323646638E-4	0.0	0.0	15.061172493819686	0.0
15	1.2612885323646638E-4	0.0	0.0	15.346349830987336	0.0
16	1.2612885323646638E-4	0.0	0.0	16.012057918369408	0.0
17	1.2612885323646638E-4	0.0	0.0	16.976186872508954	0.0
18	1.2612885323646638E-4	0.0	0.0	18.1253468543464	0.0
19	1.2612885323646638E-4	0.0	0.0	18.658745774683418	0.0
20	1.2612885323646638E-4	0.0	0.0	19.183441804147115	0.0
21	1.2612885323646638E-4	0.0	0.0	19.747363906967358	0.0
22	1.2612885323646638E-4	0.0	0.0	20.386080419756823	0.0
23	1.2612885323646638E-4	0.0	0.0	21.040184652641138	0.0
24	1.2612885323646638E-4	0.0	0.0	21.54482619444024	0.0
25	1.2612885323646638E-4	0.0	0.0	21.998007164118864	0.0
26	1.2612885323646638E-4	0.0	0.0	22.390141768831036	0.0
27	1.2612885323646638E-4	0.0	0.0	22.81343020029262	0.0
28	1.2612885323646638E-4	0.0	0.0	23.237223147167146	0.0
29	1.2612885323646638E-4	0.0	0.0	23.672746077392663	0.0
30	1.2612885323646638E-4	0.0	0.0	24.259623631501942	0.0
31	1.2612885323646638E-4	0.0	0.0	24.68417335149589	0.0
32	1.2612885323646638E-4	0.0	0.0	25.113894354472528	0.0
33	1.2612885323646638E-4	0.0	0.0	25.525705060289592	0.0
34	1.2612885323646638E-4	0.0	0.0	25.948867362897936	0.0
35	1.2612885323646638E-4	0.0	0.0	26.352353564401394	0.0
36	1.2612885323646638E-4	0.0	0.0	26.75785782755663	0.0
37	1.2612885323646638E-4	0.0	0.0	27.178623681953482	0.0
38	1.2612885323646638E-4	0.0	0.0	27.584380202815197	0.0
39	1.2612885323646638E-4	0.0	0.0	28.010695726754452	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	25	3.889707E-5	45.0	1
CCTAGCG	25	3.889707E-5	45.0	1
ACTAACG	40	6.8102963E-9	45.0	1
CGTCGGA	40	6.8102963E-9	45.0	3
TCTAGCG	100	0.0	42.75	1
TAGCCCG	45	1.9270374E-8	40.0	1
TGTCCGC	45	1.9270374E-8	40.0	21
CGCACGG	165	0.0	39.545452	2
ACGGGAC	420	0.0	39.107143	5
ACGCGAG	35	6.245835E-6	38.57143	1
GTACCGG	70	0.0	38.57143	2
ACCTAGC	35	6.245835E-6	38.57143	40
TGCGACG	35	6.245835E-6	38.57143	1
CGTACGA	35	6.245835E-6	38.57143	38
TAGGGTG	170	0.0	38.38235	5
CGAGATA	325	0.0	38.07692	19
TAGCAGG	255	0.0	37.941177	2
CTCACGG	250	0.0	37.800003	2
CCAACGG	155	0.0	37.741936	2
CACTGCG	30	1.1396023E-4	37.500004	1
>>END_MODULE