SRR3553103_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810689
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	6437	0.7940159543302054	TruSeq Adapter, Index 22 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	3309	0.40817132093811564	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	2900	0.3577204081959913	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT	2813	0.34698879595011156	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1322	0.16307116539141395	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1238	0.15270960874021974	TruSeq Adapter, Index 20 (95% over 23bp)
AATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	824	0.1016419366736196	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTC	20	7.032054E-4	45.000004	17
CTCGTGC	25	3.889786E-5	45.0	45
TAGGCGG	50	2.1827873E-11	45.0	2
CGATAGG	150	0.0	43.5	2
CGAGACA	65	0.0	41.53846	22
ACTAACG	60	3.6379788E-12	41.250004	1
CGCGACG	110	0.0	40.909092	1
ACGGGCG	255	0.0	40.588234	5
GTAAGCG	50	1.0804797E-9	40.5	1
CATAGCG	45	1.9270374E-8	40.000004	1
AGTCGCA	40	3.4565346E-7	39.375004	23
TAGGGAC	445	0.0	38.932587	5
CGCACGG	185	0.0	38.91892	2
TACGGGT	130	0.0	38.07692	4
CACGGGA	765	0.0	37.647057	4
CGTCTGA	30	1.1396249E-4	37.500004	28
TCTAGCG	60	1.546141E-10	37.500004	1
CCATACG	30	1.1396249E-4	37.500004	1
GCGGACC	30	1.1396249E-4	37.500004	5
GTAGTAC	30	1.1396249E-4	37.500004	28