##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810689
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67808493762713	31.0	31.0	34.0	30.0	34.0
2	31.882832997610674	33.0	31.0	34.0	30.0	34.0
3	32.03441517030575	33.0	31.0	34.0	30.0	34.0
4	35.71247420404126	37.0	35.0	37.0	33.0	37.0
5	35.485723871916356	37.0	35.0	37.0	33.0	37.0
6	35.517778087528015	37.0	35.0	37.0	33.0	37.0
7	35.673920578668266	37.0	35.0	37.0	35.0	37.0
8	35.75886437339103	37.0	35.0	37.0	35.0	37.0
9	37.45019853482655	39.0	37.0	39.0	35.0	39.0
10	36.92208479453897	39.0	37.0	39.0	33.0	39.0
11	36.9341584750749	39.0	37.0	39.0	33.0	39.0
12	36.93831666644052	39.0	37.0	39.0	33.0	39.0
13	36.92370317100639	39.0	37.0	39.0	33.0	39.0
14	38.09098063499073	40.0	37.0	41.0	33.0	41.0
15	38.08867395511719	40.0	37.0	41.0	33.0	41.0
16	37.97150941976516	40.0	37.0	41.0	33.0	41.0
17	37.94606316355594	40.0	37.0	41.0	33.0	41.0
18	37.88532593880021	40.0	37.0	41.0	33.0	41.0
19	37.94876950347174	40.0	37.0	41.0	33.0	41.0
20	37.88076438683638	40.0	37.0	41.0	33.0	41.0
21	37.87829488250118	40.0	37.0	41.0	33.0	41.0
22	37.852024635834454	40.0	37.0	41.0	33.0	41.0
23	37.74029621716836	39.0	37.0	41.0	33.0	41.0
24	37.617932400711	39.0	36.0	41.0	32.0	41.0
25	37.64541642972829	39.0	37.0	41.0	32.0	41.0
26	37.623979109128165	39.0	37.0	41.0	33.0	41.0
27	37.56025183516737	39.0	37.0	41.0	32.0	41.0
28	37.47094755202057	39.0	36.0	41.0	32.0	41.0
29	37.33708364119903	39.0	36.0	41.0	32.0	41.0
30	37.326413704885596	39.0	36.0	41.0	32.0	41.0
31	37.33508780802502	39.0	36.0	41.0	32.0	41.0
32	37.281810904058155	39.0	36.0	41.0	32.0	41.0
33	37.228881852350284	39.0	36.0	41.0	31.0	41.0
34	37.22799988651629	39.0	36.0	41.0	31.0	41.0
35	37.16331170152796	39.0	36.0	41.0	31.0	41.0
36	37.08510168510983	39.0	36.0	41.0	31.0	41.0
37	37.02580767717337	39.0	36.0	41.0	31.0	41.0
38	36.98349552047703	39.0	35.0	41.0	31.0	41.0
39	36.914904482483415	39.0	35.0	41.0	31.0	41.0
40	36.79721076763099	39.0	35.0	40.0	31.0	41.0
41	36.735064864578156	39.0	35.0	40.0	31.0	41.0
42	36.636548664160976	39.0	35.0	40.0	30.0	41.0
43	36.52028213038539	39.0	35.0	40.0	30.0	41.0
44	36.466334192273486	39.0	35.0	40.0	30.0	41.0
45	36.27432961345226	38.0	35.0	40.0	30.0	41.0
46	36.115296988117514	38.0	35.0	40.0	30.0	41.0
47	36.00007031056299	38.0	35.0	40.0	29.0	41.0
48	35.94853760196573	38.0	35.0	40.0	29.0	41.0
49	35.73320471845554	38.0	34.0	40.0	28.0	41.0
50	35.646134337581984	38.0	34.0	40.0	28.0	41.0
51	34.39781346484287	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	12.0
12	2.0
13	12.0
14	7.0
15	27.0
16	47.0
17	107.0
18	241.0
19	420.0
20	736.0
21	1131.0
22	1701.0
23	2299.0
24	2925.0
25	3880.0
26	4889.0
27	6186.0
28	8225.0
29	11052.0
30	14165.0
31	18508.0
32	24776.0
33	32799.0
34	48137.0
35	61082.0
36	77082.0
37	113083.0
38	175777.0
39	201313.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.200827937717175	32.66567080594408	21.717452685308423	26.41604857103032
2	21.019651185596448	30.081079180795594	22.26883552138983	26.630434112218126
3	20.740875970933367	29.574596423536033	23.01116704432896	26.67336056120164
4	19.84854858028171	30.423750661474376	20.94354308495613	28.784157673287787
5	17.661520015690357	34.217560618190205	20.132134517675706	27.988784848443732
6	19.85804667387864	34.29083162593793	22.003382308135425	23.847739392048
7	83.34688147982764	5.245044647207499	6.124790147639847	5.283283725325001
8	85.97773005431183	3.291644514727596	4.527136793517613	6.203488637442965
9	78.03041610284585	6.881553838771712	9.358829341461398	5.729200716921039
10	32.336938085998455	29.705966159649382	15.506439584106852	22.45065617024531
11	21.833650142039673	26.390884790591706	31.127843106296005	20.64762196107262
12	21.089715044856906	21.181488832338914	37.777125383470114	19.951670739334073
13	23.875616913514307	24.775222064194775	29.739517866900872	21.609643155390046
14	18.845451214954192	28.382647353054008	30.98635851726124	21.785542914730556
15	18.266807616730954	32.21901370315867	28.298521381195503	21.215657298914874
16	18.438266708935238	30.41116877125507	28.746165298900074	22.40439922090962
17	16.670634484987463	30.38637504641114	30.613712533412933	22.329277935188465
18	18.198100627984346	29.435948927393856	30.062946456656004	22.30300398796579
19	18.76736948447555	33.13366778135635	29.837582599492528	18.261380134675566
20	19.22599171815579	30.470747721999437	30.035315638919485	20.267944920925288
21	18.70816058932587	31.426724674936995	28.896284518477493	20.96883021725964
22	16.75426704938639	30.98278131317928	29.509589990736274	22.753361646698057
23	18.07869602276582	30.316927946475158	27.35228922558466	24.252086805174365
24	15.979740689709617	31.462250012026804	29.320614933716875	23.237394364546702
25	17.066840675030743	31.054078691088694	28.684982773912065	23.194097859968497
26	19.357484806134043	30.614822700196992	28.157283495890535	21.87040899777843
27	16.423313995872647	29.987578467205054	29.029504532564275	24.55960300435802
28	18.370299831378002	31.6028711380073	28.65710525244576	21.36972377816894
29	17.68643709239918	31.433138971911546	26.63314785324582	24.247276082443452
30	18.125076323966404	33.24690479333012	26.928698921534643	21.699319961168833
31	17.382991504757065	31.749043097908075	25.738230073431367	25.129735323903496
32	18.71531499748979	31.024104187919164	26.449970333876493	23.81061048071455
33	16.942255291486624	29.233775220830676	30.06936075363055	23.754608734052145
34	16.141701688316974	29.580517313050997	29.11757776409943	25.160203234532602
35	18.327496734259377	29.446063780315264	28.346381904774827	23.880057580650533
36	16.51718476505787	29.336033916828775	31.07899576779752	23.067785550315843
37	17.459593012856963	30.94528234625115	27.119647608392366	24.475477032499516
38	18.665974251531722	28.847560531843904	29.105735985069494	23.380729231554888
39	18.266437561136268	27.59911630723989	30.732006971847404	23.402439159776435
40	19.76627288639663	28.61985298924742	29.87557497388024	21.738299150475708
41	18.404961705413545	26.923518143209048	32.27624896847003	22.39527118290738
42	21.456686842920032	28.702868794321866	27.79956308769454	22.040881275063555
43	17.73294074546466	28.18133710954509	30.132640260321775	23.953081884668475
44	19.441980833587234	26.877137842008462	27.691383502181477	25.989497822222823
45	20.30495048039384	27.280621792080563	29.559794199748605	22.85463352777699
46	16.472161334371133	27.87332750290185	32.76311877921126	22.89139238351575
47	18.33514454988288	28.07032043113944	29.5498026986921	24.044732320285583
48	19.307403948986604	26.212517993953295	31.868817758721285	22.61126029833882
49	19.704226898354364	26.120497502741497	29.58064066491589	24.59463493398825
50	18.31183104741769	25.53075223667769	28.586547985725723	27.570868730178898
51	18.872465273366235	24.63213390091638	31.809115456112025	24.68628536960536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1599.0
1	1218.0
2	837.0
3	608.0
4	379.0
5	412.0
6	445.0
7	485.5
8	526.0
9	616.5
10	707.0
11	863.5
12	1020.0
13	1182.0
14	1344.0
15	1620.0
16	1896.0
17	2067.0
18	2238.0
19	2751.0
20	3264.0
21	4419.0
22	5574.0
23	6462.5
24	7351.0
25	9191.5
26	12917.5
27	14803.0
28	18302.0
29	21801.0
30	24787.5
31	27774.0
32	30576.0
33	33378.0
34	34123.5
35	34869.0
36	37933.0
37	40997.0
38	41216.0
39	41435.0
40	41442.0
41	41449.0
42	42679.5
43	43910.0
44	53333.0
45	62756.0
46	66555.0
47	70354.0
48	75484.0
49	80614.0
50	73292.0
51	65970.0
52	56776.5
53	47583.0
54	41067.0
55	34551.0
56	30158.5
57	25766.0
58	22630.5
59	19495.0
60	18948.5
61	18402.0
62	15773.0
63	13144.0
64	10726.0
65	8308.0
66	7922.5
67	7537.0
68	6530.0
69	5523.0
70	4687.0
71	3851.0
72	3278.5
73	2706.0
74	2518.0
75	1816.0
76	1302.0
77	1078.5
78	855.0
79	733.5
80	612.0
81	417.5
82	223.0
83	142.0
84	61.0
85	58.0
86	55.0
87	45.0
88	35.0
89	25.5
90	16.0
91	9.0
92	2.0
93	3.0
94	4.0
95	5.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	810689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.90746756943545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17293428147423	24.737508969669157
2	9.996619006483211	6.579268315196966
3	3.8238467696356166	3.774993406868237
4	1.9688435780976108	2.5915862478215352
5	1.2443678071014295	2.04744966283199
6	0.9169517999383118	1.8104736971523274
7	0.6796942097605287	1.5656910615379358
8	0.5694466497114341	1.4991237726322149
9	0.45976440949848	1.3616714155636684
>10	4.402848956603797	30.64784343292523
>50	0.6407003920852232	14.480546754276812
>100	0.11717824170469064	5.659172868740041
>500	0.004535931936955764	1.0033165192381117
>1k	0.0018899716403982351	1.4406659962578492
>5k	3.77994328079647E-4	0.8006878792878411
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	6437	0.7940159543302054	TruSeq Adapter, Index 22 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	3309	0.40817132093811564	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	2900	0.3577204081959913	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT	2813	0.34698879595011156	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1322	0.16307116539141395	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1238	0.15270960874021974	TruSeq Adapter, Index 20 (95% over 23bp)
AATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	824	0.1016419366736196	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.903552410357116	0.0
2	0.0	0.0	0.0	1.285943191532141	0.0
3	0.0	0.0	0.0	1.792672652521497	0.0
4	0.0	0.0	0.0	3.8664642051390854	0.0
5	0.0	0.0	0.0	4.17916118264834	0.0
6	0.0	0.0	0.0	5.7806384445823245	0.0
7	0.0	0.0	0.0	6.977521589660153	0.0
8	0.0	0.0	0.0	8.07461307603779	0.0
9	0.0	0.0	0.0	9.769220995967627	0.0
10	0.0	0.0	0.0	12.068746461343375	0.0
11	0.0	0.0	0.0	14.21642578103317	0.0
12	0.0	0.0	0.0	15.129229581257425	0.0
13	0.0	0.0	0.0	15.5307399014912	0.0
14	0.0	0.0	0.0	15.903262533474612	0.0
15	0.0	0.0	0.0	16.212999066226384	0.0
16	0.0	0.0	0.0	16.906729954396816	0.0
17	0.0	0.0	0.0	17.88750063217831	0.0
18	0.0	0.0	0.0	19.03862023538003	0.0
19	0.0	0.0	0.0	19.585192348730523	0.0
20	0.0	0.0	0.0	20.107957552156254	0.0
21	0.0	0.0	0.0	20.706460800627614	0.0
22	0.0	0.0	0.0	21.354304795057043	0.0
23	0.0	0.0	0.0	22.022501847194178	0.0
24	0.0	0.0	0.0	22.543170068916687	0.0
25	0.0	0.0	0.0	22.994021135108532	0.0
26	0.0	0.0	0.0	23.40626306758819	0.0
27	1.233518648951694E-4	0.0	0.0	23.845519058479887	0.0
28	1.233518648951694E-4	0.0	0.0	24.269109362529896	0.0
29	1.233518648951694E-4	0.0	0.0	24.713176076152507	0.0
30	1.233518648951694E-4	0.0	0.0	25.3031680459461	0.0
31	1.233518648951694E-4	0.0	0.0	25.73169242459192	0.0
32	1.233518648951694E-4	0.0	0.0	26.156762951020674	0.0
33	1.233518648951694E-4	0.0	0.0	26.581093366260056	0.0
34	1.233518648951694E-4	0.0	0.0	27.010974615419723	0.0
35	1.233518648951694E-4	0.0	0.0	27.417665713979098	0.0
36	1.233518648951694E-4	0.0	0.0	27.829660942728964	0.0
37	1.233518648951694E-4	0.0	0.0	28.237338856207497	0.0
38	1.233518648951694E-4	0.0	0.0	28.644893417821137	0.0
39	1.233518648951694E-4	0.0	0.0	29.06305623981576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTC	20	7.032054E-4	45.000004	17
CTCGTGC	25	3.889786E-5	45.0	45
TAGGCGG	50	2.1827873E-11	45.0	2
CGATAGG	150	0.0	43.5	2
CGAGACA	65	0.0	41.53846	22
ACTAACG	60	3.6379788E-12	41.250004	1
CGCGACG	110	0.0	40.909092	1
ACGGGCG	255	0.0	40.588234	5
GTAAGCG	50	1.0804797E-9	40.5	1
CATAGCG	45	1.9270374E-8	40.000004	1
AGTCGCA	40	3.4565346E-7	39.375004	23
TAGGGAC	445	0.0	38.932587	5
CGCACGG	185	0.0	38.91892	2
TACGGGT	130	0.0	38.07692	4
CACGGGA	765	0.0	37.647057	4
CGTCTGA	30	1.1396249E-4	37.500004	28
TCTAGCG	60	1.546141E-10	37.500004	1
CCATACG	30	1.1396249E-4	37.500004	1
GCGGACC	30	1.1396249E-4	37.500004	5
GTAGTAC	30	1.1396249E-4	37.500004	28
>>END_MODULE