FastQCFastQC Report
Sat 18 Jun 2016
SRR3553102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838113
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT53010.6324922772943505No Hit
AATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT34350.40984926853538844No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGT32660.3896849231547536No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC32170.3838384561509009No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16670.19889919378413173No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC11780.14055383939874455No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC253.8898987E-545.014
ATCTACG351.2113924E-745.01
CCATACG406.8121153E-945.01
GACTACG207.0321927E-445.01
GCTAACG650.041.5384641
GTAACGG850.039.7058832
TAGCGCG403.4567347E-739.3751
ATACGTG403.4567347E-739.37513
TCTACGG1100.038.8636362
CTAAGGG4400.038.3522723
GTACAGG1600.037.968752
GCATAGG6650.037.8947332
GGGCACC4050.037.7777757
TGGGACG1500.037.56
AGGGCAC3600.037.56
TAGAACC301.1396585E-437.49999636
CCTACCG1200.037.4999961
TATACGG1200.037.4999962
TAGGGCC3450.037.1739125
TGTAGGG4000.037.1253