##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553102_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 838113 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.690902062132434 31.0 31.0 34.0 30.0 34.0 2 31.89317669574389 33.0 31.0 34.0 30.0 34.0 3 32.06582286636766 34.0 31.0 34.0 30.0 34.0 4 35.738818035276864 37.0 35.0 37.0 33.0 37.0 5 35.48903310174165 37.0 35.0 37.0 33.0 37.0 6 34.87692351747318 37.0 35.0 37.0 32.0 37.0 7 35.33076446732123 37.0 35.0 37.0 33.0 37.0 8 35.73987875143328 37.0 35.0 37.0 35.0 37.0 9 37.35685880066292 39.0 37.0 39.0 35.0 39.0 10 36.924371773257306 39.0 37.0 39.0 33.0 39.0 11 36.917988385814326 39.0 37.0 39.0 33.0 39.0 12 36.85759199535146 39.0 37.0 39.0 33.0 39.0 13 36.855746182197386 39.0 37.0 39.0 33.0 39.0 14 38.0149836597213 40.0 37.0 41.0 33.0 41.0 15 38.02890898959925 40.0 37.0 41.0 33.0 41.0 16 37.89013295343229 40.0 37.0 41.0 33.0 41.0 17 37.89371242302649 40.0 37.0 41.0 33.0 41.0 18 37.856899964563254 40.0 37.0 41.0 33.0 41.0 19 37.88995397995259 40.0 37.0 41.0 33.0 41.0 20 37.80887183470487 40.0 37.0 41.0 33.0 41.0 21 37.82155150916404 40.0 37.0 41.0 33.0 41.0 22 37.80692818271522 40.0 37.0 41.0 33.0 41.0 23 37.70555283118148 39.0 37.0 41.0 33.0 41.0 24 37.588874053976014 39.0 36.0 41.0 32.0 41.0 25 37.60047153546121 39.0 36.0 41.0 32.0 41.0 26 37.545532642972965 39.0 36.0 41.0 32.0 41.0 27 37.497642919272224 39.0 36.0 41.0 32.0 41.0 28 37.39629620349523 39.0 36.0 41.0 32.0 41.0 29 37.150170681041814 39.0 36.0 41.0 31.0 41.0 30 37.26011766909713 39.0 36.0 41.0 32.0 41.0 31 37.16827086562313 39.0 36.0 41.0 31.0 41.0 32 37.18128820338069 39.0 36.0 41.0 31.0 41.0 33 36.93352924963579 39.0 36.0 41.0 31.0 41.0 34 36.92175995361007 39.0 35.0 41.0 31.0 41.0 35 36.88965211135014 39.0 35.0 41.0 31.0 41.0 36 36.80932642734333 39.0 35.0 41.0 31.0 41.0 37 36.783908613754946 39.0 35.0 41.0 31.0 41.0 38 36.60112777155348 39.0 35.0 40.0 30.0 41.0 39 36.56128946812661 39.0 35.0 40.0 30.0 41.0 40 36.49311131076597 39.0 35.0 40.0 30.0 41.0 41 36.448068458549145 39.0 35.0 40.0 30.0 41.0 42 36.35846598251071 39.0 35.0 40.0 30.0 41.0 43 36.28840502414352 39.0 35.0 40.0 30.0 41.0 44 36.22340185631293 38.0 35.0 40.0 30.0 41.0 45 36.01368550541514 38.0 35.0 40.0 29.0 41.0 46 35.86559449620755 38.0 35.0 40.0 29.0 41.0 47 35.770879344432075 38.0 34.0 40.0 28.0 41.0 48 35.657235957442495 38.0 34.0 40.0 28.0 41.0 49 35.55886258774175 38.0 34.0 40.0 28.0 41.0 50 35.47035304308608 38.0 34.0 40.0 28.0 41.0 51 34.222301765990984 37.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 4.0 11 7.0 12 6.0 13 18.0 14 23.0 15 42.0 16 72.0 17 157.0 18 283.0 19 609.0 20 1068.0 21 1844.0 22 2266.0 23 3153.0 24 3737.0 25 4596.0 26 5513.0 27 7085.0 28 9054.0 29 11641.0 30 15423.0 31 19942.0 32 26116.0 33 35273.0 34 51185.0 35 65280.0 36 80533.0 37 116850.0 38 176252.0 39 200032.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.83847882087499 32.95188119024523 20.491270270238022 25.71836971864176 2 21.956705122101674 30.07697052784052 21.485408292199264 26.480916057858543 3 20.424692135785985 28.52252619873454 23.119555477602663 27.933226187876812 4 20.728589104333185 28.991556031227294 21.21384586565296 29.066008998786558 5 17.622683337449725 33.466131655277984 20.5403090036785 28.370876003593786 6 20.012337238534663 36.06112779541661 21.0846270133025 22.84190795274623 7 82.96697462036742 5.673817253759338 6.432664807728791 4.926543318144451 8 85.77876730226114 3.351815328004696 4.524568882716292 6.3448484870178605 9 78.04914134490218 6.637291152863635 9.34086453735952 5.972702964874665 10 34.13787878245535 27.637204052436843 15.957752713536241 22.267164451571567 11 23.66733364116772 27.574086071925862 28.700664468872333 20.057915818034083 12 22.325867752916373 21.289611305396765 37.32754413784299 19.056976803843874 13 25.15818272715016 24.293382873192517 29.05037864822524 21.498055751432084 14 19.88538538359386 28.07115508290648 30.487416374641608 21.556043158858053 15 18.700819579221417 31.00608151884054 29.210380939085777 21.082717962852264 16 19.433179058193822 30.87769787606206 27.404657844467273 22.284465221276843 17 18.2178298153113 30.332306025559795 29.00945337919827 22.44041077993063 18 19.814034623016227 29.205369681653902 28.819622175052768 22.1609735202771 19 19.658327695668724 32.71146014916843 29.163012624789257 18.46719953037359 20 19.63720882506297 30.903947319752827 28.731209276076136 20.727634579108067 21 20.14227198480396 31.17550974629913 28.200493250910082 20.481725017986836 22 17.902120597103256 30.92769113472766 28.78752626435815 22.382662003810943 23 18.955558498675 29.462494914170286 27.431145919464324 24.150800667690394 24 16.54311530784035 31.4038799064088 28.650909841513016 23.40209494423783 25 19.26231904289756 30.620095380933122 28.113750771077406 22.003834805091916 26 20.130459735143113 30.59026646764816 27.763917276071364 21.515356521137363 27 17.47389671798433 29.086531291126615 28.84897382572517 24.590598165163886 28 21.995959971984686 28.876297110294196 28.63134207439808 20.496400843323038 29 19.152906588968314 31.150214827833477 27.852449490701137 21.844429092497073 30 20.216367005403807 29.548879447043536 28.517634256955805 21.717119290596855 31 21.562963466740165 31.222639429289366 25.72159124127654 21.492805862693935 32 21.066848980984666 27.51335440447768 27.74434950895643 23.67544710558123 33 21.1828237958366 27.47923012767968 30.259762108450772 21.078183968032953 34 20.006490771530807 27.968304989899927 29.430995581741364 22.594208656827895 35 21.995840656331545 26.215438729622377 30.55399450909364 21.234726104952433 36 19.69829843947057 28.519781938712324 32.788776692403054 18.993142929414052 37 21.54303775266581 28.556292528573117 28.781918428660575 21.118751290100498 38 22.437308572948993 28.341047090308823 29.170768142243347 20.05087619449883 39 20.026058538645746 28.03380928347371 29.91422397695776 22.025908200922785 40 21.459516795467913 29.860770564351107 28.253469400904173 20.426243239276804 41 20.4774296544738 27.79326892674377 30.497677520811635 21.2316238979708 42 22.86505518945536 27.78551340928968 28.773447017287644 20.575984383967317 43 20.101346715776987 25.95664307796204 31.160953236616063 22.781056969644904 44 21.78847005117448 24.793673406807912 29.110036474795166 24.307820067222437 45 23.295068803371382 25.897104567045254 30.169678790330185 20.63814783925318 46 18.441308033642244 27.261717692005732 32.41424485719706 21.882729417154966 47 20.410374257409202 28.010900678070854 29.592548976092726 21.986176088427218 48 19.94683294496088 26.780040400280154 32.28073064133356 20.992396013425395 49 21.24797014245096 25.845798836195122 30.110259595066534 22.795971426287387 50 19.82811387008673 25.19469331701095 29.065173789214583 25.912019023687737 51 20.113397596744115 24.66863060231735 31.482031659215405 23.735940141723134 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2030.0 1 1567.0 2 1104.0 3 819.0 4 534.0 5 573.5 6 613.0 7 660.5 8 708.0 9 868.5 10 1029.0 11 1146.0 12 1263.0 13 1343.0 14 1423.0 15 1587.5 16 1752.0 17 2013.5 18 2275.0 19 2944.0 20 3613.0 21 4670.5 22 5728.0 23 6951.0 24 8174.0 25 9865.0 26 13316.0 27 15076.0 28 17643.0 29 20210.0 30 22253.0 31 24296.0 32 26912.5 33 29529.0 34 33197.5 35 36866.0 36 37736.5 37 38607.0 38 39336.5 39 40066.0 40 40888.5 41 41711.0 42 42638.5 43 43566.0 44 52788.5 45 62011.0 46 66136.0 47 70261.0 48 74858.0 49 79455.0 50 74748.0 51 70041.0 52 60728.5 53 51416.0 54 44479.0 55 37542.0 56 33789.5 57 30037.0 58 26050.5 59 22064.0 60 21172.5 61 20281.0 62 18998.5 63 17716.0 64 15055.0 65 12394.0 66 10981.5 67 9569.0 68 8241.0 69 6913.0 70 6351.5 71 5790.0 72 4833.5 73 3877.0 74 3161.0 75 2261.0 76 2077.0 77 1577.5 78 1078.0 79 860.5 80 643.0 81 562.0 82 481.0 83 314.0 84 147.0 85 111.5 86 76.0 87 55.0 88 34.0 89 26.0 90 18.0 91 14.0 92 10.0 93 7.0 94 4.0 95 3.0 96 2.0 97 1.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 838113.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.15891308533839 #Duplication Level Percentage of deduplicated Percentage of total 1 75.02240129054573 24.12638882556039 2 10.046795753660707 6.46188062856243 3 3.775584209902187 3.642560533578614 4 1.9414348275395388 2.4973773551877185 5 1.2698681775579148 2.041879017596102 6 0.8971932010180083 1.7311654903376796 7 0.6907708722532512 1.5550108309872988 8 0.5631787363750591 1.4488972827676985 9 0.4666788464029445 1.350709601421445 >10 4.487119958067972 31.04110865042186 >50 0.7358494375388153 16.165945452870226 >100 0.09632525908486204 4.819681090666297 >500 0.004532953368699386 0.9229977801356646 >1k 0.0018887305702914108 1.5504370449949312 >5k 3.7774611405828217E-4 0.6439604149117082 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT 5301 0.6324922772943505 No Hit AATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT 3435 0.40984926853538844 No Hit AATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGT 3266 0.3896849231547536 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC 3217 0.3838384561509009 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1667 0.19889919378413173 No Hit CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC 1178 0.14055383939874455 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1931565313985107E-4 0.0 0.0 0.7055134570159394 0.0 2 1.1931565313985107E-4 0.0 0.0 1.0354212379476275 0.0 3 1.1931565313985107E-4 0.0 0.0 1.4937126616577956 0.0 4 1.1931565313985107E-4 0.0 0.0 3.416961674619055 0.0 5 1.1931565313985107E-4 0.0 0.0 3.7234835875353323 0.0 6 1.1931565313985107E-4 0.0 0.0 5.252036419909964 0.0 7 1.1931565313985107E-4 0.0 0.0 6.3654900950110544 0.0 8 1.1931565313985107E-4 0.0 0.0 7.334691145466065 0.0 9 1.1931565313985107E-4 0.0 0.0 8.91729396871305 0.0 10 1.1931565313985107E-4 0.0 0.0 11.068674510477704 0.0 11 1.1931565313985107E-4 0.0 0.0 13.182589937156445 0.0 12 1.1931565313985107E-4 0.0 0.0 14.02925381183683 0.0 13 1.1931565313985107E-4 0.0 0.0 14.414762687131688 0.0 14 1.1931565313985107E-4 0.0 0.0 14.79645346152607 0.0 15 1.1931565313985107E-4 0.0 0.0 15.145929009572695 0.0 16 1.1931565313985107E-4 0.0 0.0 15.813142141930742 0.0 17 1.1931565313985107E-4 0.0 0.0 16.697509763003318 0.0 18 1.1931565313985107E-4 0.0 0.0 17.892098082239507 0.0 19 2.3863130627970215E-4 0.0 0.0 18.38606488623849 0.0 20 2.3863130627970215E-4 0.0 0.0 18.983239730203444 0.0 21 2.3863130627970215E-4 0.0 0.0 19.51729659365742 0.0 22 2.3863130627970215E-4 0.0 0.0 20.114829384581792 0.0 23 2.3863130627970215E-4 0.0 0.0 20.722861952982473 0.0 24 2.3863130627970215E-4 0.0 0.0 21.1816306393052 0.0 25 2.3863130627970215E-4 0.0 0.0 21.586349334755575 0.0 26 2.3863130627970215E-4 0.0 0.0 21.957063069061093 0.0 27 2.3863130627970215E-4 0.0 0.0 22.43766651990841 0.0 28 2.3863130627970215E-4 0.0 0.0 22.845487422340423 0.0 29 2.3863130627970215E-4 0.0 0.0 23.251995852587896 0.0 30 2.3863130627970215E-4 0.0 0.0 23.941282380776816 0.0 31 2.3863130627970215E-4 0.0 0.0 24.34791012667743 0.0 32 2.3863130627970215E-4 0.0 0.0 24.725066906252497 0.0 33 2.3863130627970215E-4 0.0 0.0 25.096377218823715 0.0 34 2.3863130627970215E-4 0.0 0.0 25.446687976442316 0.0 35 2.3863130627970215E-4 0.0 0.0 25.809884824600026 0.0 36 2.3863130627970215E-4 0.0 0.0 26.173081672757732 0.0 37 3.5794695941955323E-4 0.0 0.0 26.51838117294446 0.0 38 3.5794695941955323E-4 0.0 0.0 26.876447448017153 0.0 39 3.5794695941955323E-4 0.0 0.0 27.244536237953593 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCGTC 25 3.8898987E-5 45.0 14 ATCTACG 35 1.2113924E-7 45.0 1 CCATACG 40 6.8121153E-9 45.0 1 GACTACG 20 7.0321927E-4 45.0 1 GCTAACG 65 0.0 41.538464 1 GTAACGG 85 0.0 39.705883 2 TAGCGCG 40 3.4567347E-7 39.375 1 ATACGTG 40 3.4567347E-7 39.375 13 TCTACGG 110 0.0 38.863636 2 CTAAGGG 440 0.0 38.352272 3 GTACAGG 160 0.0 37.96875 2 GCATAGG 665 0.0 37.894733 2 GGGCACC 405 0.0 37.777775 7 TGGGACG 150 0.0 37.5 6 AGGGCAC 360 0.0 37.5 6 TAGAACC 30 1.1396585E-4 37.499996 36 CCTACCG 120 0.0 37.499996 1 TATACGG 120 0.0 37.499996 2 TAGGGCC 345 0.0 37.173912 5 TGTAGGG 400 0.0 37.125 3 >>END_MODULE