SRR3553100_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	559755
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	4350	0.7771257067824315	RNA PCR Primer, Index 33 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	4232	0.7560450554260346	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGT	4057	0.7247813775669713	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	3684	0.6581450813302249	TruSeq Adapter, Index 1 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1021	0.18240122910916381	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	1009	0.18025743405597092	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	983	0.17561254477405294	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	945	0.16882386043894204	No Hit
AGGCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTC	843	0.15060160248680227	No Hit
AGGCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAAC	777	0.13881072969424121	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	707	0.1263052585506159	Illumina Paired End PCR Primer 2 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCGAACTC	698	0.1246974122607212	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTAT	666	0.11898062545220678	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTACG	20	7.0301816E-4	45.000004	1
CATAGAT	20	7.0301816E-4	45.000004	26
GTATCAC	20	7.0301816E-4	45.000004	38
TATAGCG	35	1.2104283E-7	45.000004	1
CCGAACG	20	7.0301816E-4	45.000004	1
CGTATCG	25	3.888232E-5	45.0	27
AATCACG	55	1.8189894E-12	45.0	1
CGTCAAC	25	3.888232E-5	45.0	17
CGTACGG	85	0.0	42.352943	2
CGGTAGT	85	0.0	42.352943	12
CGCCGGT	85	0.0	42.352943	28
CCACGCG	55	6.002665E-11	40.909092	1
GGCAACC	100	0.0	40.5	8
GGTACCG	40	3.453988E-7	39.375004	7
ATATTGG	40	3.453988E-7	39.375004	2
GCCAGGG	1195	0.0	38.786613	3
CGACCGG	35	6.242546E-6	38.57143	2
TCCACCG	35	6.242546E-6	38.57143	1
ATCGGGT	35	6.242546E-6	38.57143	4
TAACGGG	305	0.0	38.360657	3