##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	559755
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.705004868201268	31.0	31.0	34.0	30.0	34.0
2	31.914666237907657	33.0	31.0	34.0	30.0	34.0
3	32.07832712526016	34.0	31.0	34.0	30.0	34.0
4	35.73913051245634	37.0	35.0	37.0	33.0	37.0
5	35.49390894230512	37.0	35.0	37.0	33.0	37.0
6	34.8112995864262	37.0	35.0	37.0	32.0	37.0
7	35.244215772972105	37.0	35.0	37.0	33.0	37.0
8	35.688997865137424	37.0	35.0	37.0	33.0	37.0
9	37.33016408964637	39.0	37.0	39.0	34.0	39.0
10	36.92150315763146	39.0	37.0	39.0	33.0	39.0
11	36.921067252637314	39.0	37.0	39.0	33.0	39.0
12	36.82997740082715	39.0	37.0	39.0	33.0	39.0
13	36.856839152843655	39.0	37.0	39.0	33.0	39.0
14	38.00121839018856	40.0	37.0	41.0	33.0	41.0
15	38.05838625827371	40.0	37.0	41.0	33.0	41.0
16	37.89082902341203	40.0	37.0	41.0	33.0	41.0
17	37.8755169672446	40.0	37.0	41.0	33.0	41.0
18	37.83587283722343	40.0	37.0	41.0	33.0	41.0
19	37.842456074532606	40.0	37.0	41.0	33.0	41.0
20	37.80942555225054	40.0	37.0	41.0	33.0	41.0
21	37.81914051683326	40.0	37.0	41.0	33.0	41.0
22	37.79811346035319	40.0	37.0	41.0	33.0	41.0
23	37.682239551232236	39.0	37.0	41.0	33.0	41.0
24	37.56854695357791	39.0	36.0	41.0	32.0	41.0
25	37.56987253351913	39.0	36.0	41.0	32.0	41.0
26	37.5526328482997	39.0	36.0	41.0	33.0	41.0
27	37.48240033586122	39.0	36.0	41.0	32.0	41.0
28	37.380032335575386	39.0	36.0	41.0	32.0	41.0
29	37.14841671802842	39.0	36.0	41.0	31.0	41.0
30	37.218606354565836	39.0	36.0	41.0	31.0	41.0
31	37.111120043590496	39.0	36.0	41.0	31.0	41.0
32	37.123848826718834	39.0	36.0	41.0	31.0	41.0
33	36.790420809103985	39.0	35.0	41.0	31.0	41.0
34	36.833805861492976	39.0	35.0	41.0	31.0	41.0
35	36.786622718868074	39.0	35.0	41.0	31.0	41.0
36	36.71337638788398	39.0	35.0	40.0	31.0	41.0
37	36.65162794436852	39.0	35.0	40.0	30.0	41.0
38	36.42484837116238	39.0	35.0	40.0	30.0	41.0
39	36.329931845182266	39.0	35.0	40.0	30.0	41.0
40	36.23054729301212	39.0	35.0	40.0	30.0	41.0
41	36.276256576537946	39.0	35.0	40.0	30.0	41.0
42	36.29045743226948	39.0	35.0	40.0	30.0	41.0
43	36.23604791381944	38.0	35.0	40.0	30.0	41.0
44	36.14421487972417	38.0	35.0	40.0	30.0	41.0
45	35.904372448660574	38.0	35.0	40.0	29.0	41.0
46	35.672771123080636	38.0	34.0	40.0	28.0	41.0
47	35.55318844851765	38.0	34.0	40.0	28.0	41.0
48	35.47294262668489	38.0	34.0	40.0	28.0	41.0
49	35.38972943519933	38.0	34.0	40.0	27.0	41.0
50	35.29882537896044	38.0	34.0	40.0	27.0	41.0
51	34.007474698752134	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	11.0
11	8.0
12	12.0
13	21.0
14	20.0
15	36.0
16	53.0
17	106.0
18	198.0
19	424.0
20	852.0
21	1295.0
22	1783.0
23	2175.0
24	2673.0
25	3039.0
26	3740.0
27	4553.0
28	5796.0
29	7705.0
30	10269.0
31	13512.0
32	18036.0
33	24147.0
34	35755.0
35	45218.0
36	55637.0
37	79064.0
38	115031.0
39	128549.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.064233459281294	34.650338094344846	20.231172566569303	26.054255879804554
2	22.402836955453726	30.64912327714804	21.04081249832516	25.907227269073076
3	21.57997695420318	28.173218640297986	23.63962805155827	26.607176353940567
4	20.963993175585745	28.822788541415438	20.33871961840448	29.874498664594327
5	17.453707425570116	35.11232592830792	18.643335030504417	28.790631615617546
6	19.88423506712758	36.07042366749739	21.408116050772215	22.637225214602818
7	81.91958982054649	6.954828451733347	6.241480647783406	4.884101079936758
8	84.15270966762243	3.3796929013586303	4.606479620548275	7.8611178104706525
9	77.59537654866861	7.005207635483381	9.228859054407732	6.170556761440273
10	34.4931264571107	29.424301703423822	15.134478477190912	20.948093362274566
11	23.467231199364008	27.385195308661824	28.644496252824897	20.50307723914927
12	22.622754597993765	21.010978017168224	36.62673848380095	19.73952890103706
13	24.977713463926182	24.567355360827506	28.71488419040473	21.740046984841584
14	19.278970263776117	27.74463827924717	30.98248340791954	21.993908049057175
15	18.39733454815053	30.59534975123045	28.700056274620145	22.307259425998875
16	19.449580620092718	29.449312645711068	27.77929629927379	23.321810434922423
17	17.85370385257836	28.896570821162832	29.590803119221803	23.658922207037005
18	21.28359728809926	27.71426784932694	28.42207751605613	22.580057346517673
19	20.471456262114675	30.841886182347633	29.717287027360186	18.969370528177507
20	20.222061437593233	30.97498012523336	27.314628721494227	21.488329715679182
21	20.098971871622407	29.99294334128324	28.010826165018628	21.89725862207573
22	18.775535725451313	30.55711873944851	27.31016248180007	23.357183053300105
23	19.468338826808157	29.403399701655186	26.126966262025352	25.001295209511305
24	16.583147984386027	31.70190529785353	27.93757983403453	23.777366883725914
25	19.470661271449117	29.358558655125904	27.353395682039466	23.817384391385517
26	20.66707756071853	30.243946012094575	26.31794267134729	22.77103375583961
27	18.67245491331029	28.52140668685407	28.036909004832474	24.76922939500317
28	20.40106832453484	28.033157363489387	29.40732999258604	22.158444319389734
29	20.85644612375057	32.23195862475548	25.093299747210835	21.818295504283125
30	21.77434770569267	28.355977168582687	26.806370644299736	23.06330448142491
31	20.225634429348556	31.727809488079608	24.188082286000125	23.858473796571715
32	23.195147877196273	28.56928477637538	25.062036069351766	23.173531277076577
33	20.46216648355084	29.43716447374298	26.600030370429923	23.500638672276263
34	19.487990281462427	29.758555082134148	27.45004510902091	23.303409527382517
35	20.462345133138605	28.42940214915454	27.29068967673357	23.817563040973283
36	20.55095532867058	31.547909353199167	26.618073978794293	21.28306133933596
37	21.647149199203223	29.946673098051825	24.22935034077409	24.17682736197086
38	22.55647560093255	29.17526417807791	25.977972505828443	22.290287715161096
39	21.135318130253413	29.2585148859769	25.865065966360284	23.741101017409402
40	21.75791194361819	30.72880099329171	25.153504658288	22.3597824048021
41	18.128466918562587	29.298889692812036	29.014836848263975	23.55780654036141
42	21.665192807567596	27.78001089762485	26.350992845084008	24.20380344972354
43	19.625550464042306	27.403060267438434	28.503363078489695	24.468026190029565
44	21.880108261650186	25.35984493215782	26.97841019731847	25.781636608873526
45	22.940393565041852	26.866218256201375	27.4437923734491	22.749595805307678
46	19.815454975837643	26.89962572911363	31.297978579914425	21.986940715134303
47	20.048414038284605	29.437343123330745	26.30222150762387	24.21202133076078
48	20.14667131155595	26.604496610124073	30.73684022474118	22.5119918535788
49	21.421693419442434	25.885432019365616	27.64638100597583	25.04649355521612
50	20.202052683763434	25.55975382086806	26.84906789577583	27.389125599592678
51	20.179542835704904	24.762083411492526	30.342024635778152	24.716349117024414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1221.0
1	983.0
2	745.0
3	551.0
4	357.0
5	371.5
6	386.0
7	442.0
8	498.0
9	637.5
10	777.0
11	881.5
12	986.0
13	1000.0
14	1014.0
15	1123.5
16	1233.0
17	1517.0
18	1801.0
19	1834.0
20	1867.0
21	2432.0
22	2997.0
23	3966.0
24	4935.0
25	5602.5
26	7381.0
27	8492.0
28	9728.5
29	10965.0
30	12024.5
31	13084.0
32	14819.0
33	16554.0
34	18744.0
35	20934.0
36	21784.0
37	22634.0
38	23184.5
39	23735.0
40	24838.0
41	25941.0
42	26575.0
43	27209.0
44	35785.5
45	44362.0
46	47212.5
47	50063.0
48	52608.5
49	55154.0
50	51420.0
51	47686.0
52	42666.5
53	37647.0
54	32462.5
55	27278.0
56	24253.0
57	21228.0
58	19250.0
59	17272.0
60	15983.0
61	14694.0
62	13723.0
63	12752.0
64	10795.5
65	8839.0
66	7954.0
67	7069.0
68	6376.0
69	5683.0
70	5231.5
71	4780.0
72	4066.5
73	3353.0
74	3092.0
75	2410.5
76	1990.0
77	1663.0
78	1336.0
79	922.5
80	509.0
81	374.5
82	240.0
83	212.5
84	185.0
85	121.0
86	57.0
87	55.0
88	53.0
89	42.5
90	32.0
91	26.5
92	21.0
93	11.0
94	1.0
95	2.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	559755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.0827186037963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89794541761134	25.86808316274738
2	9.42854094979676	6.427006160725865
3	3.668262433067846	3.750730688133857
4	1.9648702034149745	2.678724729439063
5	1.355847745086883	2.310548858269399
6	0.9229916648777949	1.8874839112607604
7	0.7214714126012439	1.721279499546013
8	0.564878953248068	1.5402088327008727
9	0.4598960857956148	1.410705799124337
>10	4.389894605932237	32.046340814736475
>50	0.5570973991674208	12.440198558618938
>100	0.060832474621728735	3.4664724861811
>500	0.004268945587489735	1.1143272257519203
>1k	0.0032017091906173015	3.337889272763994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	4350	0.7771257067824315	RNA PCR Primer, Index 33 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	4232	0.7560450554260346	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGT	4057	0.7247813775669713	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	3684	0.6581450813302249	TruSeq Adapter, Index 1 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1021	0.18240122910916381	No Hit
AACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	1009	0.18025743405597092	Illumina Paired End PCR Primer 2 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	983	0.17561254477405294	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	945	0.16882386043894204	No Hit
AGGCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTC	843	0.15060160248680227	No Hit
AGGCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAAC	777	0.13881072969424121	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	707	0.1263052585506159	Illumina Paired End PCR Primer 2 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCGAACTC	698	0.1246974122607212	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTAT	666	0.11898062545220678	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7514001661441166	0.0
2	0.0	0.0	0.0	1.056890961224107	0.0
3	0.0	0.0	0.0	1.5930183741101018	0.0
4	0.0	0.0	0.0	3.7066216469705497	0.0
5	0.0	0.0	0.0	4.020151673500013	0.0
6	0.0	0.0	0.0	5.534385579405275	0.0
7	0.0	0.0	0.0	6.655411742637404	0.0
8	0.0	0.0	0.0	7.763575135550375	0.0
9	0.0	0.0	0.0	9.69424123053836	0.0
10	0.0	0.0	0.0	11.707443435074273	0.0
11	0.0	0.0	0.0	14.13940027333387	0.0
12	0.0	0.0	0.0	15.000133987190825	0.0
13	0.0	0.0	0.0	15.45247474341453	0.0
14	0.0	0.0	0.0	15.907495243454726	0.0
15	0.0	0.0	0.0	16.230850997311325	0.0
16	0.0	0.0	0.0	16.888817429053784	0.0
17	0.0	0.0	0.0	17.762949861993192	0.0
18	0.0	0.0	0.0	19.004028548204126	0.0
19	0.0	0.0	0.0	19.47637805825763	0.0
20	0.0	0.0	0.0	20.103438111316557	0.0
21	0.0	0.0	0.0	20.610802940572214	0.0
22	0.0	0.0	0.0	21.168189654402372	0.0
23	0.0	0.0	0.0	21.712892247501138	0.0
24	0.0	0.0	0.0	22.151476985466857	0.0
25	0.0	0.0	0.0	22.548972318246374	0.0
26	0.0	0.0	0.0	22.875007815919464	0.0
27	0.0	0.0	0.0	23.318416092754866	0.0
28	0.0	0.0	0.0	23.690900483247134	0.0
29	0.0	0.0	0.0	24.085001473859098	0.0
30	0.0	0.0	0.0	24.757617171798376	0.0
31	0.0	0.0	0.0	25.09937383319488	0.0
32	0.0	0.0	0.0	25.4668560352297	0.0
33	0.0	0.0	0.0	25.801288063527792	0.0
34	0.0	0.0	0.0	26.12482246697216	0.0
35	0.0	0.0	0.0	26.480156497038884	0.0
36	0.0	0.0	0.0	26.816017722039106	0.0
37	0.0	0.0	0.0	27.150449750337202	0.0
38	0.0	0.0	0.0	27.49881644648105	0.0
39	0.0	0.0	0.0	27.849684236853623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTACG	20	7.0301816E-4	45.000004	1
CATAGAT	20	7.0301816E-4	45.000004	26
GTATCAC	20	7.0301816E-4	45.000004	38
TATAGCG	35	1.2104283E-7	45.000004	1
CCGAACG	20	7.0301816E-4	45.000004	1
CGTATCG	25	3.888232E-5	45.0	27
AATCACG	55	1.8189894E-12	45.0	1
CGTCAAC	25	3.888232E-5	45.0	17
CGTACGG	85	0.0	42.352943	2
CGGTAGT	85	0.0	42.352943	12
CGCCGGT	85	0.0	42.352943	28
CCACGCG	55	6.002665E-11	40.909092	1
GGCAACC	100	0.0	40.5	8
GGTACCG	40	3.453988E-7	39.375004	7
ATATTGG	40	3.453988E-7	39.375004	2
GCCAGGG	1195	0.0	38.786613	3
CGACCGG	35	6.242546E-6	38.57143	2
TCCACCG	35	6.242546E-6	38.57143	1
ATCGGGT	35	6.242546E-6	38.57143	4
TAACGGG	305	0.0	38.360657	3
>>END_MODULE