FastQCFastQC Report
Sat 18 Jun 2016
SRR3553098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745746
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT50710.6799902379630598Illumina PCR Primer Index 1 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT14980.20087268319240065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14710.19725214751403292No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC12260.16439913858069638No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGT11320.15179431066341623No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC9740.1306074722492645TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTA207.0316903E-445.00000442
TCTAGCG302.1644719E-645.0000041
AGTTGCG207.0316903E-445.0000041
TCCGTAG302.1644719E-645.0000041
TCGGTAC207.0316903E-445.00000442
AGTCGGC207.0316903E-445.00000430
CCTAGCG253.889483E-545.01
CGCCCTC253.889483E-545.010
ATACCCG603.6379788E-1241.2500041
CGGGATA2300.040.10876
TTGGCGG451.9266736E-840.02
TTGCCGG451.9266736E-840.02
ATAGGCG403.4560435E-739.3750041
ACGCATG356.2453364E-638.571431
CGCGACT356.2453364E-638.571439
GATAGGG3850.038.571433
AACGGGA5900.038.516954
ACTAGGG5850.038.4615363
TAGGGTC2050.038.414635
CGAACAG659.094947E-1238.076921
Produced by FastQC (version 0.11.5)