##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745746
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.625993300668057	31.0	31.0	34.0	30.0	34.0
2	31.837962255245085	33.0	31.0	34.0	30.0	34.0
3	32.01793506099932	33.0	31.0	34.0	30.0	34.0
4	35.70418748474682	37.0	35.0	37.0	33.0	37.0
5	35.46186369085453	37.0	35.0	37.0	33.0	37.0
6	34.762844185553796	37.0	35.0	37.0	32.0	37.0
7	35.283331053736795	37.0	35.0	37.0	33.0	37.0
8	35.72233575506942	37.0	35.0	37.0	35.0	37.0
9	37.3724217092683	39.0	37.0	39.0	35.0	39.0
10	36.880161609985166	39.0	37.0	39.0	32.0	39.0
11	36.89041711252893	39.0	37.0	39.0	33.0	39.0
12	36.80170460183494	39.0	37.0	39.0	33.0	39.0
13	36.759715774539856	39.0	35.0	39.0	33.0	39.0
14	37.90566627243056	40.0	37.0	41.0	33.0	41.0
15	37.8964499977204	40.0	37.0	41.0	33.0	41.0
16	37.79070085525098	40.0	37.0	41.0	33.0	41.0
17	37.781518104019334	39.0	37.0	41.0	33.0	41.0
18	37.76623273876092	39.0	37.0	41.0	33.0	41.0
19	37.80853936863222	40.0	37.0	41.0	33.0	41.0
20	37.71119389175403	39.0	36.0	41.0	33.0	41.0
21	37.69158667964696	39.0	36.0	41.0	33.0	41.0
22	37.67535058853819	39.0	36.0	41.0	33.0	41.0
23	37.56940298707603	39.0	36.0	41.0	32.0	41.0
24	37.4258138830111	39.0	36.0	41.0	32.0	41.0
25	37.4822727845674	39.0	36.0	41.0	32.0	41.0
26	37.4379413365945	39.0	36.0	41.0	32.0	41.0
27	37.332968597887216	39.0	36.0	41.0	32.0	41.0
28	37.272590131224305	39.0	36.0	41.0	32.0	41.0
29	37.035309609438066	39.0	36.0	41.0	31.0	41.0
30	37.06768122122009	39.0	36.0	41.0	31.0	41.0
31	37.03444068087526	39.0	35.0	41.0	31.0	41.0
32	36.911213737653306	39.0	35.0	41.0	31.0	41.0
33	36.75172511820379	39.0	35.0	40.0	31.0	41.0
34	36.714890860963386	39.0	35.0	40.0	30.0	41.0
35	36.69363831653136	39.0	35.0	40.0	30.0	41.0
36	36.662124905799025	39.0	35.0	40.0	30.0	41.0
37	36.557980867480346	39.0	35.0	40.0	30.0	41.0
38	36.373468982736746	39.0	35.0	40.0	30.0	41.0
39	36.31588369230274	39.0	35.0	40.0	30.0	41.0
40	36.28256537748778	39.0	35.0	40.0	30.0	41.0
41	36.240819260177055	38.0	35.0	40.0	30.0	41.0
42	36.145672923488696	38.0	35.0	40.0	30.0	41.0
43	36.029693756319176	38.0	35.0	40.0	29.0	41.0
44	35.907811775054775	38.0	35.0	40.0	29.0	41.0
45	35.65522175110561	38.0	34.0	40.0	28.0	41.0
46	35.479299922493716	38.0	34.0	40.0	28.0	41.0
47	35.383548822253154	38.0	34.0	40.0	27.0	41.0
48	35.2486583903903	38.0	34.0	40.0	27.0	41.0
49	35.123736500095205	37.0	34.0	40.0	26.0	41.0
50	35.01609529249905	37.0	34.0	40.0	26.0	41.0
51	33.67104215108093	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	10.0
12	9.0
13	11.0
14	23.0
15	33.0
16	70.0
17	118.0
18	278.0
19	562.0
20	968.0
21	1471.0
22	2089.0
23	2790.0
24	3621.0
25	4493.0
26	5646.0
27	7058.0
28	8955.0
29	11550.0
30	14734.0
31	19247.0
32	24860.0
33	33640.0
34	49506.0
35	61239.0
36	73108.0
37	104535.0
38	150189.0
39	164882.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.04462645458373	31.52775878113996	22.156203318556184	26.271411445720123
2	21.992474649545557	29.43294365641921	21.809302363002953	26.765279331032282
3	20.458976648885812	29.611154468143308	21.997167936536034	27.932700946434846
4	19.61177130014777	30.1752875643986	21.22451880398956	28.988422331464065
5	17.88785994158869	33.826530749075424	19.84040142354099	28.445207885794893
6	19.627862569829404	37.21267026574731	20.458708461057785	22.70075870336549
7	83.51261689636954	5.2446811649006495	6.218873450209589	5.02382848852022
8	86.16217854336463	3.4328041987486353	4.640185800527257	5.764831457359476
9	77.89140002091865	6.772413127257806	9.689089850968024	5.64709700085552
10	34.52516004108638	27.879733850399468	15.49052894685322	22.10457716166094
11	24.65289790357575	26.818380520981677	28.727877856535606	19.800843718906975
12	23.4452481139691	21.21969142308507	35.61695268898526	19.718107773960572
13	25.14059746884328	24.162114178285904	29.40853856406873	21.288749788802086
14	20.300745830349744	27.857742448501234	29.94612106534933	21.895390655799694
15	19.520453344704496	30.278405784275076	29.47116042191309	20.729980449107337
16	20.292834289422938	29.992785747426066	27.59304106223835	22.121338900912644
17	18.678075376870947	30.228388754347996	29.851718949883743	21.241816918897317
18	19.939094544255013	29.150407779592513	29.627648019566983	21.282849656585487
19	19.537080990042185	33.30088260614203	28.971258310470322	18.190778093345454
20	20.14653782923408	30.914681406269693	29.07424243643278	19.864538328063443
21	19.427392168379047	32.54083830151285	27.992372738170907	20.0393967919372
22	18.15095220088341	30.77911245920193	28.780979046484994	22.288956293429667
23	19.23644243482365	30.409415538266384	27.257940371118316	23.096201655791653
24	16.650709490899047	31.129633950433526	28.845746406953577	23.373910151713854
25	19.241940285298213	30.499928930225572	27.25405164761192	23.004079136864295
26	19.887200199531744	29.801031450386596	28.25640365486372	22.055364695217943
27	17.970327698707067	29.620943323866307	27.64131487128325	24.767414106143377
28	20.22753055329831	30.07538759845846	28.212018569325213	21.485063278918023
29	19.19929842064188	32.27627100916398	25.297487348239212	23.22694322195493
30	20.8231757193468	31.830676932896722	25.335033644163023	22.01111370359345
31	20.371547416948935	30.649309550436744	24.50217634422444	24.476966688389883
32	21.503434145137888	31.198692316150538	25.363729741761944	21.93414379694963
33	19.374827354085706	29.105620412311968	26.24298353594924	25.27656869765309
34	18.06379115677456	29.909111145081567	28.56776972320334	23.459327974940532
35	18.665604642867677	30.198351717608947	26.75642913270738	24.379614506815994
36	17.901403426904068	31.74552729749808	28.15650368892357	22.196565586674282
37	17.78366897040011	32.8839846274737	25.0610797778332	24.271266624292988
38	18.418335465426566	32.646772493583605	27.209130186417358	21.725761854572468
39	17.48088491255736	30.805931242004654	27.657003859222844	24.056179986215145
40	19.37455916625768	30.078471758480767	27.513255183400247	23.033713891861304
41	17.123792819539094	29.116750207175095	30.168180586955877	23.59127638632993
42	20.174965739004968	29.317488796453482	27.684761299423666	22.82278416511788
43	17.934926905407472	27.83065547787048	28.663110496067024	25.571307120655025
44	20.53688521292773	27.088579757719117	27.09582082907585	25.278714200277307
45	20.97899284743063	27.00611200060074	28.52392101332089	23.490974138647744
46	17.49241698916253	28.905820480431675	30.3111246992944	23.290637831111397
47	18.300064633266555	28.40752749595707	28.294218138615562	24.998189732160817
48	19.08773228418255	26.585995767996074	31.97201191826708	22.3542600295543
49	20.189313787804426	27.120762297082386	28.390899850619377	24.29902406449381
50	17.690071418418604	26.368629533380002	28.18157925084412	27.75971979735728
51	19.41948062745224	25.05169320385225	30.956250519613913	24.57257564908159
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1795.0
1	1449.5
2	1104.0
3	801.0
4	498.0
5	554.5
6	611.0
7	681.0
8	751.0
9	944.5
10	1138.0
11	1262.0
12	1386.0
13	1570.5
14	1755.0
15	1896.5
16	2038.0
17	2156.0
18	2274.0
19	2695.5
20	3117.0
21	3825.5
22	4534.0
23	6159.0
24	7784.0
25	8760.0
26	11367.5
27	12999.0
28	15109.5
29	17220.0
30	18796.0
31	20372.0
32	23476.0
33	26580.0
34	28674.5
35	30769.0
36	31945.5
37	33122.0
38	34943.5
39	36765.0
40	36122.0
41	35479.0
42	36946.5
43	38414.0
44	44375.0
45	50336.0
46	57183.0
47	64030.0
48	67776.0
49	71522.0
50	66914.0
51	62306.0
52	55501.0
53	48696.0
54	42117.0
55	35538.0
56	31173.0
57	26808.0
58	25253.0
59	23698.0
60	21344.0
61	18990.0
62	17427.5
63	15865.0
64	13439.0
65	11013.0
66	9641.5
67	8270.0
68	6971.0
69	5672.0
70	4890.5
71	4109.0
72	3553.5
73	2998.0
74	2489.5
75	1660.0
76	1339.0
77	1146.5
78	954.0
79	757.5
80	561.0
81	437.0
82	313.0
83	253.5
84	194.0
85	217.5
86	241.0
87	146.5
88	52.0
89	31.0
90	10.0
91	8.5
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	745746.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.04010108550297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77548296672501	25.794050999901273
2	9.86749905112775	6.717813303229864
3	3.712306667497959	3.7910188266605154
4	1.9530295586806985	2.659252944018649
5	1.290241651296478	2.195997811742919
6	0.8769184791278476	1.7910236203954475
7	0.6541608445209589	1.5587390891569999
8	0.5458364776066068	1.4864263099107018
9	0.4584835258485634	1.4046143009330618
>10	4.118400531480095	29.914877525115823
>50	0.6100648146801904	14.312343863665355
>100	0.13316339133349694	6.404626075780671
>500	0.002406567313255969	0.5495452888575156
>1k	0.0016043782088373127	0.7273112431662075
>5k	4.010945522093282E-4	0.6923587974649594
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	5071	0.6799902379630598	Illumina PCR Primer Index 1 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	1498	0.20087268319240065	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1471	0.19725214751403292	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	1226	0.16439913858069638	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGT	1132	0.15179431066341623	No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	974	0.1306074722492645	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.801211136231371	0.0
2	0.0	0.0	0.0	1.133764042985145	0.0
3	0.0	0.0	0.0	1.6012154272366195	0.0
4	0.0	0.0	0.0	3.3421567128754295	0.0
5	0.0	0.0	0.0	3.6167810487753203	0.0
6	0.0	0.0	0.0	5.1677112582568325	0.0
7	0.0	0.0	0.0	6.405398084602532	0.0
8	0.0	0.0	0.0	7.781738018038313	0.0
9	0.0	0.0	0.0	9.377053313058333	0.0
10	0.0	0.0	0.0	11.850281463125514	0.0
11	0.0	0.0	0.0	13.830178103536593	0.0
12	0.0	0.0	0.0	14.791631467014238	0.0
13	0.0	0.0	0.0	15.235080040657275	0.0
14	0.0	0.0	0.0	15.61469991122983	0.0
15	0.0	0.0	0.0	15.945509597101426	0.0
16	0.0	0.0	0.0	16.612895007147205	0.0
17	0.0	0.0	0.0	17.513872015404708	0.0
18	0.0	0.0	0.0	18.655279411488632	0.0
19	0.0	0.0	0.0	19.187632250122697	0.0
20	0.0	0.0	0.0	19.78515473096738	0.0
21	0.0	0.0	0.0	20.352237893330972	0.0
22	0.0	0.0	0.0	20.961962920350896	0.0
23	0.0	0.0	0.0	21.58576780834225	0.0
24	0.0	0.0	0.0	22.073199185781753	0.0
25	0.0	0.0	0.0	22.49586320275268	0.0
26	0.0	0.0	0.0	22.89076977952279	0.0
27	0.0	0.0	0.0	23.33488882273589	0.0
28	0.0	0.0	0.0	23.744277542219468	0.0
29	0.0	0.0	0.0	24.197112689843458	0.0
30	0.0	0.0	0.0	24.845993139755358	0.0
31	0.0	0.0	0.0	25.254577295754856	0.0
32	0.0	0.0	0.0	25.679923191006054	0.0
33	0.0	0.0	0.0	26.08569137481126	0.0
34	0.0	0.0	0.0	26.473088692396608	0.0
35	0.0	0.0	0.0	26.889182107580865	0.0
36	0.0	0.0	0.0	27.30366639579696	0.0
37	0.0	0.0	0.0	27.695086530802712	0.0
38	0.0	0.0	0.0	28.153285434987247	0.0
39	0.0	0.0	0.0	28.609338836547565	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	20	7.0316903E-4	45.000004	42
TCTAGCG	30	2.1644719E-6	45.000004	1
AGTTGCG	20	7.0316903E-4	45.000004	1
TCCGTAG	30	2.1644719E-6	45.000004	1
TCGGTAC	20	7.0316903E-4	45.000004	42
AGTCGGC	20	7.0316903E-4	45.000004	30
CCTAGCG	25	3.889483E-5	45.0	1
CGCCCTC	25	3.889483E-5	45.0	10
ATACCCG	60	3.6379788E-12	41.250004	1
CGGGATA	230	0.0	40.1087	6
TTGGCGG	45	1.9266736E-8	40.0	2
TTGCCGG	45	1.9266736E-8	40.0	2
ATAGGCG	40	3.4560435E-7	39.375004	1
ACGCATG	35	6.2453364E-6	38.57143	1
CGCGACT	35	6.2453364E-6	38.57143	9
GATAGGG	385	0.0	38.57143	3
AACGGGA	590	0.0	38.51695	4
ACTAGGG	585	0.0	38.461536	3
TAGGGTC	205	0.0	38.41463	5
CGAACAG	65	9.094947E-12	38.07692	1
>>END_MODULE