##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553096_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1159491
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.918547017613765	33.0	31.0	34.0	30.0	34.0
2	32.126071698702276	34.0	31.0	34.0	30.0	34.0
3	32.28447827538118	34.0	31.0	34.0	30.0	34.0
4	35.90861852312782	37.0	35.0	37.0	35.0	37.0
5	35.691309376269416	37.0	35.0	37.0	33.0	37.0
6	35.022734975950655	37.0	35.0	37.0	33.0	37.0
7	35.488465197228784	37.0	35.0	37.0	35.0	37.0
8	35.89778445887031	37.0	35.0	37.0	35.0	37.0
9	37.61333895649039	39.0	37.0	39.0	35.0	39.0
10	37.11493750274905	39.0	37.0	39.0	33.0	39.0
11	37.121641306400825	39.0	37.0	39.0	34.0	39.0
12	37.04211761885172	39.0	37.0	39.0	33.0	39.0
13	37.04372435836069	39.0	37.0	39.0	33.0	39.0
14	38.25410546524294	40.0	38.0	41.0	33.0	41.0
15	38.2729646025713	40.0	38.0	41.0	33.0	41.0
16	38.17088791547326	40.0	37.0	41.0	33.0	41.0
17	38.1619943578691	40.0	37.0	41.0	33.0	41.0
18	38.143836390278146	40.0	37.0	41.0	33.0	41.0
19	38.1802937668339	40.0	37.0	41.0	34.0	41.0
20	38.10381365616465	40.0	37.0	41.0	33.0	41.0
21	38.120039741576264	40.0	37.0	41.0	33.0	41.0
22	38.11789655978356	40.0	37.0	41.0	33.0	41.0
23	38.02138955800433	40.0	37.0	41.0	33.0	41.0
24	37.87868038647993	40.0	37.0	41.0	33.0	41.0
25	37.94802029511225	40.0	37.0	41.0	33.0	41.0
26	37.90481168029765	40.0	37.0	41.0	33.0	41.0
27	37.848090239596516	40.0	37.0	41.0	33.0	41.0
28	37.82472740193757	40.0	37.0	41.0	33.0	41.0
29	37.63018427913627	40.0	36.0	41.0	33.0	41.0
30	37.68014240731493	40.0	37.0	41.0	33.0	41.0
31	37.636884632998445	40.0	36.0	41.0	33.0	41.0
32	37.63800408972558	40.0	37.0	41.0	33.0	41.0
33	37.41301916099392	40.0	36.0	41.0	32.0	41.0
34	37.41415931645869	40.0	36.0	41.0	32.0	41.0
35	37.35967851410662	40.0	36.0	41.0	32.0	41.0
36	37.370903267037	40.0	36.0	41.0	32.0	41.0
37	37.303609083641014	39.0	36.0	41.0	32.0	41.0
38	37.130455518844045	39.0	35.0	41.0	32.0	41.0
39	37.131637071784084	39.0	35.0	41.0	32.0	41.0
40	37.06298884596776	39.0	35.0	41.0	31.0	41.0
41	37.0389231136766	39.0	35.0	41.0	31.0	41.0
42	37.00533682452041	39.0	35.0	41.0	31.0	41.0
43	36.94272314317231	39.0	35.0	41.0	31.0	41.0
44	36.86455177314873	39.0	35.0	41.0	31.0	41.0
45	36.68993549755884	39.0	35.0	40.0	31.0	41.0
46	36.59450396768927	39.0	35.0	40.0	31.0	41.0
47	36.487208611364814	39.0	35.0	40.0	31.0	41.0
48	36.41268453140214	39.0	35.0	40.0	30.0	41.0
49	36.31196533651404	38.0	35.0	40.0	30.0	41.0
50	36.23763961945371	38.0	35.0	40.0	30.0	41.0
51	35.08964105801597	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	15.0
11	6.0
12	12.0
13	12.0
14	19.0
15	45.0
16	79.0
17	166.0
18	332.0
19	560.0
20	965.0
21	1496.0
22	2173.0
23	2910.0
24	3829.0
25	4805.0
26	5767.0
27	7549.0
28	9945.0
29	12767.0
30	17096.0
31	22529.0
32	30619.0
33	42388.0
34	66988.0
35	84666.0
36	101863.0
37	150934.0
38	248284.0
39	340576.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.34168441152195	31.799470629784963	21.559546387164712	26.299298571528368
2	22.9336838319573	30.971434879615277	19.943492446254435	26.15138884217299
3	21.576019132533155	29.780395018158828	21.79395959088945	26.849626258418564
4	21.6201764394894	29.741067416650928	20.321589387067256	28.31716675679242
5	18.893117755980857	33.57102383718373	17.915878605353555	29.619979801481854
6	19.87190931193084	37.48610381624351	19.663197040770473	22.97878983105518
7	86.3177894438163	4.706375469926028	4.8084029975221885	4.167432088735488
8	88.1096964098902	3.319042579890659	3.7589769993902498	4.81228401082889
9	81.11559296277419	6.722346270906803	7.357883761064123	4.804177005254892
10	33.158429000311344	32.636044609229394	15.453246295141575	18.752280095317687
11	25.315591065389896	31.95358998043107	24.461509403695242	18.26930955048379
12	27.200124882383736	24.34059427800647	29.020923836407526	19.438357003202267
13	25.683769861085597	24.705237039356064	27.48136898000933	22.12962411954901
14	21.185675438619185	26.803657811919194	29.922008881483343	22.088657867978277
15	20.060699048116803	28.917171414008386	29.55909101493673	21.46303852293808
16	22.844420525903175	26.57976646649263	28.53898822845542	22.03682477914878
17	21.465884599362997	27.833161275076733	28.332518320538924	22.368435805021342
18	22.93687488734281	27.377357823389747	26.901373102507907	22.784394186759535
19	23.181033746704376	30.530120544273302	26.102660563988856	20.186185145033466
20	24.399758169748623	28.704060661100435	26.17519239045409	20.720988778696857
21	24.493592447030636	27.46179142399553	26.621077697023953	21.423538431949883
22	22.606040064131587	27.126730608517015	26.17881466954034	24.088414657811057
23	23.112210444065543	28.376761872235317	25.018391690836754	23.492635992862386
24	21.023880306099834	28.44868998551951	27.919147281005202	22.60828242737546
25	23.03234781468765	27.574944523070897	25.235728436012007	24.156979226229442
26	22.671758556125056	28.900698668639947	24.854699174034124	23.572843601200873
27	20.37971834192762	27.35519292517148	27.2030572035488	25.0620315293521
28	21.476578947141462	28.57460730613692	27.977879949046606	21.970933797675013
29	23.00957920328834	28.420401710750664	25.618913816493617	22.95110526946738
30	22.512809500030617	26.046342748671613	28.816265068034163	22.624582683263604
31	22.541356509019906	28.54580156292718	25.56733946188457	23.345502466168345
32	24.264267682974687	26.5499257864011	26.223575689677624	22.96223084094659
33	22.112375171519226	26.525173545978365	28.05067050973229	23.31178077277012
34	21.583177445965514	26.624354997149613	29.077155406984616	22.715312149900257
35	22.739978145582846	25.098081830734348	29.184961332170754	22.976978691512052
36	21.60568732314438	28.537780802093334	28.488448810728155	21.36808306403413
37	22.74049561402374	28.9716780897825	26.68550251791519	21.602323778278574
38	23.97991877470373	27.273519156250458	27.87533495300955	20.871227116036263
39	22.649593657906788	25.602441071125178	28.014275229389447	23.73369004157859
40	22.830880101699798	25.334564908222664	29.263271556225966	22.571283433851576
41	21.334620104856356	25.747159745095043	31.36471089469431	21.55350925535429
42	23.562149253422408	26.726813748446514	26.706201255550926	23.00483574258015
43	20.810424574231277	26.726727503706364	27.73009881059879	24.732749111463566
44	22.782151823515665	25.28678532218016	27.24100488921432	24.690057965089853
45	23.553524779407518	25.57975870446601	28.294053166432516	22.57266334969396
46	21.135912223553266	25.99787320470793	29.727354502967252	23.138860068771557
47	21.784300179992773	27.23315661786077	27.641094238765113	23.341448963381346
48	21.02543271142251	25.49118535633308	30.616451529162365	22.86693040308204
49	22.156187499514875	25.85927790728863	28.369776048283256	23.614758544913244
50	21.208702784238948	24.740769872297413	28.24334125922495	25.807186084238687
51	21.160750708716154	23.782245830282424	31.071565022928166	23.98543843807326
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2212.0
1	1820.5
2	1429.0
3	1020.0
4	611.0
5	639.0
6	667.0
7	727.0
8	787.0
9	867.0
10	947.0
11	1030.0
12	1113.0
13	1221.5
14	1330.0
15	1331.5
16	1333.0
17	1520.5
18	1708.0
19	1879.0
20	2050.0
21	2809.5
22	3569.0
23	4449.5
24	5330.0
25	6471.5
26	8986.5
27	10360.0
28	13427.5
29	16495.0
30	19196.5
31	21898.0
32	24519.5
33	27141.0
34	30563.0
35	33985.0
36	38912.5
37	43840.0
38	46849.0
39	49858.0
40	53083.5
41	56309.0
42	65998.0
43	75687.0
44	82912.5
45	90138.0
46	99002.0
47	107866.0
48	103951.5
49	100037.0
50	94015.0
51	87993.0
52	81991.5
53	75990.0
54	69082.0
55	62174.0
56	60464.5
57	58755.0
58	54222.5
59	49690.0
60	47443.0
61	45196.0
62	41126.5
63	37057.0
64	31480.0
65	25903.0
66	21155.5
67	16408.0
68	14234.0
69	12060.0
70	10103.0
71	8146.0
72	6819.0
73	5492.0
74	4445.5
75	3099.5
76	2800.0
77	2239.0
78	1678.0
79	1352.0
80	1026.0
81	834.5
82	643.0
83	473.0
84	303.0
85	263.0
86	223.0
87	140.5
88	58.0
89	50.0
90	42.0
91	62.5
92	83.0
93	67.5
94	52.0
95	28.5
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1159491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.985545979475006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66321474217196	17.668524241943064
2	10.702137419876397	5.1339321832621065
3	3.969326124544506	2.8561936280338074
4	1.9996354764077369	1.9184939464626873
5	1.1745739157080777	1.4086398330754046
6	0.7446566003763038	1.0716597076347227
7	0.5297782583924211	0.8894914542918323
8	0.4223085219397401	0.8103440376407813
9	0.3501688996974163	0.7559093019847095
>10	4.475823436378857	26.223715171537563
>50	1.5993059995364485	27.2317976581692
>100	0.3613586818813289	11.730389910485444
>500	0.0051412820592871495	0.772225271788976
>1k	0.0025706410296435747	1.5286836536897088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	4626	0.3989681679288585	TruSeq Adapter, Index 20 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	3146	0.2713259525084714	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGT	2632	0.22699615607193158	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCC	2612	0.22527126126895336	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1724	0.14868593201672112	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1600	0.13799158423825628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.624474014891017E-5	0.0	0.0	0.44243551696390915	0.0
2	8.624474014891017E-5	0.0	0.0	0.648042977478911	0.0
3	8.624474014891017E-5	0.0	0.0	0.9220425169319986	0.0
4	8.624474014891017E-5	0.0	0.0	2.0972995909411973	0.0
5	8.624474014891017E-5	0.0	0.0	2.2925576826383303	0.0
6	8.624474014891017E-5	0.0	0.0	3.3175764193081276	0.0
7	8.624474014891017E-5	0.0	0.0	4.0559176397229475	0.0
8	8.624474014891017E-5	0.0	0.0	4.728281633923851	0.0
9	8.624474014891017E-5	0.0	0.0	5.711989140062321	0.0
10	8.624474014891017E-5	0.0	0.0	7.041193075237324	0.0
11	8.624474014891017E-5	0.0	0.0	8.333225527408148	0.0
12	8.624474014891017E-5	0.0	0.0	8.877947306188664	0.0
13	8.624474014891017E-5	0.0	0.0	9.131334352746162	0.0
14	8.624474014891017E-5	0.0	0.0	9.414734568875481	0.0
15	8.624474014891017E-5	0.0	0.0	9.653028785906919	0.0
16	8.624474014891017E-5	0.0	0.0	10.111505824538526	0.0
17	8.624474014891017E-5	0.0	0.0	10.665197056294529	0.0
18	8.624474014891017E-5	0.0	0.0	11.377923588885123	0.0
19	8.624474014891017E-5	0.0	0.0	11.704359930348748	0.0
20	8.624474014891017E-5	0.0	0.0	12.07538480246936	0.0
21	8.624474014891017E-5	0.0	0.0	12.507557195355549	0.0
22	8.624474014891017E-5	0.0	0.0	12.966637947168197	0.0
23	8.624474014891017E-5	0.0	0.0	13.424080048918016	0.0
24	8.624474014891017E-5	0.0	0.0	13.761038248679808	0.0
25	8.624474014891017E-5	0.0	0.0	14.056771462650422	0.0
26	8.624474014891017E-5	0.0	0.0	14.32663125457636	0.0
27	8.624474014891017E-5	0.0	0.0	14.628919068798291	0.0
28	8.624474014891017E-5	0.0	0.0	14.938796420153325	0.0
29	8.624474014891017E-5	0.0	0.0	15.267906348561567	0.0
30	8.624474014891017E-5	0.0	0.0	15.726297142453024	0.0
31	8.624474014891017E-5	0.0	0.0	16.03393213056419	0.0
32	8.624474014891017E-5	0.0	0.0	16.353813871776495	0.0
33	8.624474014891017E-5	0.0	0.0	16.688702197774713	0.0
34	8.624474014891017E-5	0.0	0.0	16.998148325429003	0.0
35	8.624474014891017E-5	0.0	0.0	17.3347615462302	0.0
36	8.624474014891017E-5	0.0	0.0	17.665682614181566	0.0
37	8.624474014891017E-5	0.0	0.0	17.968229162623945	0.0
38	8.624474014891017E-5	0.0	0.0	18.30958584413333	0.0
39	8.624474014891017E-5	0.0	0.0	18.654306070508525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	25	3.8908263E-5	45.000004	1
CGCGAAT	25	3.8908263E-5	45.000004	11
CGCTAAG	65	0.0	45.000004	1
ACTACCG	100	0.0	45.000004	1
ACGTATA	25	3.8908263E-5	45.000004	42
ATACGTT	25	3.8908263E-5	45.000004	44
ATCGCGC	25	3.8908263E-5	45.000004	1
GATACGT	45	3.8562575E-10	45.0	11
CACGATC	20	7.033312E-4	45.0	35
ATATCCG	35	1.211938E-7	45.0	1
CATACCG	55	1.8189894E-12	45.0	1
TATAGCG	45	3.8562575E-10	45.0	1
TCATACG	45	3.8562575E-10	45.0	1
GCGCTAA	20	7.033312E-4	45.0	15
TATAACG	20	7.033312E-4	45.0	1
TATCGCG	45	3.8562575E-10	45.0	1
GTCGGAC	20	7.033312E-4	45.0	45
CGTACCG	30	2.1655196E-6	44.999996	1
GTGCGAC	30	2.1655196E-6	44.999996	42
TTTCGCG	85	0.0	42.35294	1
>>END_MODULE