FastQCFastQC Report
Sat 18 Jun 2016
SRR3553095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176330
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT49310.4191850926185679TruSeq Adapter, Index 20 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT34150.290309692008195No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCC30690.2608961770931626No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGT29860.2538403339199034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17590.14953286917786676No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG15800.1343160507680668No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC253.890861E-545.00000442
CGAGTTA253.890861E-545.00000416
CGAAACG502.1827873E-1145.0000041
TAACGCG351.2119563E-745.0000041
TTATCGG253.890861E-545.0000042
CCGTATC253.890861E-545.00000441
CGAGGTA253.890861E-545.00000443
CGTAACG502.1827873E-1145.0000041
CACCGTA253.890861E-545.00000425
TATCCCG253.890861E-545.0000041
TATCACG453.8562575E-1045.01
GATCGGT207.0333545E-445.09
TAGCGAA207.0333545E-445.032
TAACGGT207.0333545E-445.022
CTAGTAT207.0333545E-445.043
TTGACGT207.0333545E-445.039
ACTACGT207.0333545E-445.022
ACGTCGC207.0333545E-445.045
TCATACG453.8562575E-1045.01
TATCGCG207.0333545E-445.01