##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1176330
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.927007727423426	33.0	31.0	34.0	30.0	34.0
2	32.132492582863655	34.0	31.0	34.0	30.0	34.0
3	32.27357034165583	34.0	31.0	34.0	30.0	34.0
4	35.90004505538412	37.0	35.0	37.0	35.0	37.0
5	35.69817143148606	37.0	35.0	37.0	33.0	37.0
6	35.726960971836135	37.0	35.0	37.0	33.0	37.0
7	35.84358470837265	37.0	35.0	37.0	35.0	37.0
8	35.89638536805148	37.0	35.0	37.0	35.0	37.0
9	37.63853850535139	39.0	37.0	39.0	35.0	39.0
10	37.12834663742317	39.0	37.0	39.0	33.0	39.0
11	37.12851580763901	39.0	37.0	39.0	34.0	39.0
12	37.08282879804137	39.0	37.0	39.0	33.0	39.0
13	37.0762031062712	39.0	37.0	39.0	33.0	39.0
14	38.278869874950054	40.0	38.0	41.0	33.0	41.0
15	38.29707480043865	40.0	38.0	41.0	33.0	41.0
16	38.2028223372693	40.0	37.0	41.0	33.0	41.0
17	38.163258609403826	40.0	37.0	41.0	33.0	41.0
18	38.11487082706383	40.0	37.0	41.0	33.0	41.0
19	38.17390358147799	40.0	37.0	41.0	34.0	41.0
20	38.10727601948433	40.0	37.0	41.0	33.0	41.0
21	38.108647233344385	40.0	37.0	41.0	33.0	41.0
22	38.09244259689033	40.0	37.0	41.0	33.0	41.0
23	37.96434078872425	40.0	37.0	41.0	33.0	41.0
24	37.841521511820666	40.0	37.0	41.0	33.0	41.0
25	37.916563379323826	40.0	37.0	41.0	33.0	41.0
26	37.85833227070635	40.0	37.0	41.0	33.0	41.0
27	37.83114602194962	40.0	37.0	41.0	33.0	41.0
28	37.78509856927903	40.0	37.0	41.0	33.0	41.0
29	37.6437003221885	40.0	36.0	41.0	33.0	41.0
30	37.66411041119414	40.0	37.0	41.0	33.0	41.0
31	37.60842535683014	40.0	36.0	41.0	33.0	41.0
32	37.60412384279921	40.0	36.0	41.0	33.0	41.0
33	37.51179855992791	40.0	36.0	41.0	33.0	41.0
34	37.4672132819872	40.0	36.0	41.0	33.0	41.0
35	37.38614164392645	40.0	36.0	41.0	32.0	41.0
36	37.359516462217236	40.0	36.0	41.0	32.0	41.0
37	37.285384203412306	39.0	36.0	41.0	32.0	41.0
38	37.211274047248644	39.0	36.0	41.0	32.0	41.0
39	37.15234585532971	39.0	35.0	41.0	32.0	41.0
40	37.06312174304829	39.0	35.0	41.0	31.0	41.0
41	37.00032048829835	39.0	35.0	41.0	31.0	41.0
42	36.95917217107444	39.0	35.0	41.0	31.0	41.0
43	36.887257827310364	39.0	35.0	41.0	31.0	41.0
44	36.82256169612268	39.0	35.0	41.0	31.0	41.0
45	36.6587717732269	39.0	35.0	40.0	31.0	41.0
46	36.56848588406314	39.0	35.0	40.0	31.0	41.0
47	36.434928974012394	39.0	35.0	40.0	30.0	41.0
48	36.37989679766732	39.0	35.0	40.0	30.0	41.0
49	36.22244098169731	38.0	35.0	40.0	30.0	41.0
50	36.16271879489599	38.0	35.0	40.0	30.0	41.0
51	34.93838208665935	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	6.0
11	8.0
12	5.0
13	20.0
14	27.0
15	73.0
16	86.0
17	181.0
18	302.0
19	573.0
20	946.0
21	1449.0
22	2201.0
23	2945.0
24	3909.0
25	4821.0
26	6162.0
27	7763.0
28	9994.0
29	13174.0
30	17310.0
31	23015.0
32	31011.0
33	42586.0
34	67689.0
35	84414.0
36	101514.0
37	150805.0
38	254779.0
39	348467.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.293115027245754	31.998163780571776	21.452568581945542	26.25615261023692
2	22.828883051524656	31.228566813734243	19.997959756190863	25.944590378550238
3	21.663393775556177	29.677216427363067	21.8138617564799	26.845528040600854
4	21.69221221936021	29.625275220388836	20.387136262783404	28.295376297467545
5	18.836465957681945	33.691141091360414	17.9615414041978	29.510851546759838
6	20.337660350411873	36.184829087075904	20.039529723801994	23.437980838710224
7	86.13407802232366	4.743651866398035	4.88417365875222	4.238096452526077
8	87.74060000170022	3.4006613790347946	3.8661770081524742	4.992561611112528
9	80.91504934839713	6.721328198719748	7.467717392228371	4.895905060654748
10	33.00782943561756	32.57130227062134	15.572500913859205	18.848367379901898
11	25.374682274531807	31.812161553305618	24.515994661362033	18.297161510800542
12	27.130227062133926	24.257138728078008	29.252165633793236	19.360468575994833
13	25.750172145571398	24.55773464929059	27.495345693810407	22.1967475113276
14	21.06951280678041	26.76493841014001	30.09521137775964	22.070337405319936
15	19.902748378431223	28.902093800209126	29.636751591815226	21.55840622954443
16	22.72092014995792	26.56813989271718	28.706995485960572	22.003944471364328
17	21.364328037200444	27.76771824232996	28.434452917123597	22.433500803346
18	22.735201856621867	27.45675108175427	26.989620259621024	22.81842680200284
19	23.04863431180026	30.529953329422867	26.287776389278516	20.133635969498357
20	24.36841702583459	28.711160983737553	26.19732558040686	20.723096410020997
21	24.38023343789583	27.474688225242915	26.60758460636046	21.537493730500795
22	22.42474475699846	27.198150178946385	26.267203930869737	24.109901133185417
23	23.16841362542824	28.279224367311894	25.064990266336828	23.48737174092304
24	20.86956891348516	28.536805148215212	27.933233021346048	22.660392916953576
25	22.996693104826026	27.442044324296756	25.295112765975535	24.266149804901683
26	22.59850552140981	28.869534909421674	24.8256016593983	23.70635790977022
27	20.1826018209176	27.35218858653609	27.278484778931084	25.186724813615225
28	21.450953388929978	28.37129036919912	28.083105931158777	22.09465031071213
29	22.97425042292554	28.353693266345324	25.700696233199867	22.971360077529265
30	22.458238759531763	26.05357340202154	28.827964941810546	22.66022289663615
31	22.34866066494946	28.639752450417827	25.645779670670642	23.36580721396207
32	24.33849344996727	26.457881716865167	26.13628828645023	23.067336546717332
33	22.04534441865803	26.48151454098765	28.255846573665554	23.21729446668877
34	21.532648151454097	26.50990793399811	29.10798840461435	22.849455509933435
35	22.82463254358896	24.912482041603973	29.21425110300681	23.04863431180026
36	21.46259978067379	28.498295546317788	28.646553263114942	21.392551409893482
37	22.71811481472036	28.938138107503846	26.74751132760365	21.596235750172145
38	23.95203726845358	27.233174364336538	27.9925701121284	20.82221825508148
39	22.57283245347819	25.583552234492025	28.082510860047776	23.761104451982014
40	22.833728630571354	25.23424549233633	29.3578332610747	22.574192616017612
41	21.30822133244923	25.615771084644617	31.511395611775605	21.56461197113055
42	23.60375064820246	26.621696292706982	26.741390596176245	23.03316246291432
43	20.7450290309692	26.536431103516872	27.838531704538696	24.880008160975237
44	22.833643620412637	25.044587828245472	27.30509295860855	24.816675592733333
45	23.508114219649247	25.5481029983083	28.37112034888169	22.57266243316076
46	21.093825712172602	25.840877984919196	29.842051125109453	23.223245177798745
47	21.76795627077436	27.123511259595524	27.72733841694082	23.381194052689295
48	21.11839364804094	25.239346101859173	30.742903777001352	22.899356473098536
49	22.142085979274523	25.691600146217475	28.453835233310382	23.712478641197624
50	21.233242372463508	24.54549318643578	28.256781685411408	25.964482755689307
51	21.18070609437828	23.515085052663792	31.152992782637522	24.1512160703204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2236.0
1	1852.0
2	1468.0
3	1047.5
4	627.0
5	667.0
6	707.0
7	768.5
8	830.0
9	895.5
10	961.0
11	1071.5
12	1182.0
13	1263.0
14	1344.0
15	1350.0
16	1356.0
17	1563.0
18	1770.0
19	1839.5
20	1909.0
21	2808.0
22	3707.0
23	4594.0
24	5481.0
25	6572.0
26	8999.0
27	10335.0
28	13441.5
29	16548.0
30	19276.0
31	22004.0
32	24585.5
33	27167.0
34	30809.0
35	34451.0
36	39154.5
37	43858.0
38	47152.5
39	50447.0
40	53505.0
41	56563.0
42	66866.0
43	77169.0
44	85213.0
45	93257.0
46	101419.0
47	109581.0
48	106127.0
49	102673.0
50	96132.0
51	89591.0
52	83292.5
53	76994.0
54	69969.0
55	62944.0
56	60979.5
57	59015.0
58	54624.5
59	50234.0
60	48073.0
61	45912.0
62	41585.0
63	37258.0
64	31719.0
65	26180.0
66	21360.0
67	16540.0
68	14335.5
69	12131.0
70	10104.0
71	8077.0
72	6879.5
73	5682.0
74	4649.0
75	3177.5
76	2739.0
77	2182.5
78	1626.0
79	1317.0
80	1008.0
81	814.0
82	620.0
83	485.0
84	350.0
85	288.5
86	227.0
87	150.5
88	74.0
89	65.0
90	56.0
91	72.0
92	88.0
93	75.5
94	63.0
95	35.0
96	7.0
97	4.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1176330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.180844294438668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.98213581880498	17.889505068045374
2	10.607728946097433	5.1300768392638405
3	3.971480116861547	2.881012269728645
4	1.9656921363535271	1.9012838191986858
5	1.1069879815822377	1.3383952009227515
6	0.7632728218872169	1.1073948756138967
7	0.5365674666269328	0.9082258054777095
8	0.4394088854535975	0.8500222272597023
9	0.3374834034962062	0.7344570268709083
>10	4.339482606488683	25.616681257326384
>50	1.5581777570335247	26.723825443466993
>100	0.3841402283225499	12.522960623877372
>500	0.0049612206609921045	0.7879228622610078
>1k	0.0024806103304960522	1.6082366806867425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	4931	0.4191850926185679	TruSeq Adapter, Index 20 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	3415	0.290309692008195	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCC	3069	0.2608961770931626	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGT	2986	0.2538403339199034	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1759	0.14953286917786676	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1580	0.1343160507680668	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.47495175673492984	0.0
2	0.0	0.0	0.0	0.6850118589171406	0.0
3	0.0	0.0	0.0	0.9782118963216104	0.0
4	0.0	0.0	0.0	2.2273511684646317	0.0
5	0.0	0.0	0.0	2.428740234458018	0.0
6	0.0	0.0	0.0	3.491197198065169	0.0
7	0.0	0.0	0.0	4.251273027126742	0.0
8	0.0	0.0	0.0	4.929739103822907	0.0
9	0.0	0.0	0.0	5.990920915049348	0.0
10	0.0	0.0	0.0	7.375141329388862	0.0
11	0.0	0.0	0.0	8.737429122780172	0.0
12	0.0	0.0	0.0	9.300111363307915	0.0
13	0.0	0.0	0.0	9.576139348652164	0.0
14	0.0	0.0	0.0	9.874780036214327	0.0
15	0.0	0.0	0.0	10.116463917438134	0.0
16	0.0	0.0	0.0	10.583764759888806	0.0
17	0.0	0.0	0.0	11.143131604226705	0.0
18	0.0	0.0	0.0	11.904482585668987	0.0
19	0.0	0.0	0.0	12.229391412273767	0.0
20	0.0	0.0	0.0	12.601395866806083	0.0
21	0.0	0.0	0.0	13.045573946086558	0.0
22	0.0	0.0	0.0	13.523841099011332	0.0
23	8.501015871396631E-5	0.0	0.0	13.994712368127992	0.0
24	8.501015871396631E-5	0.0	0.0	14.328292230921596	0.0
25	8.501015871396631E-5	0.0	0.0	14.639259391497284	0.0
26	8.501015871396631E-5	0.0	0.0	14.930504195251332	0.0
27	8.501015871396631E-5	0.0	0.0	15.249292290428706	0.0
28	8.501015871396631E-5	0.0	0.0	15.57454115766834	0.0
29	8.501015871396631E-5	0.0	0.0	15.919427371570903	0.0
30	8.501015871396631E-5	0.0	0.0	16.3923388844967	0.0
31	1.7002031742793263E-4	0.0	0.0	16.71282718284835	0.0
32	1.7002031742793263E-4	0.0	0.0	17.038076050087984	0.0
33	1.7002031742793263E-4	0.0	0.0	17.373271105897153	0.0
34	1.7002031742793263E-4	0.0	0.0	17.691209099487388	0.0
35	2.5503047614189897E-4	0.0	0.0	18.031844805454252	0.0
36	2.5503047614189897E-4	0.0	0.0	18.365254647930428	0.0
37	2.5503047614189897E-4	0.0	0.0	18.683957732949086	0.0
38	2.5503047614189897E-4	0.0	0.0	19.036409850977194	0.0
39	2.5503047614189897E-4	0.0	0.0	19.38852192837044	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCC	25	3.890861E-5	45.000004	42
CGAGTTA	25	3.890861E-5	45.000004	16
CGAAACG	50	2.1827873E-11	45.000004	1
TAACGCG	35	1.2119563E-7	45.000004	1
TTATCGG	25	3.890861E-5	45.000004	2
CCGTATC	25	3.890861E-5	45.000004	41
CGAGGTA	25	3.890861E-5	45.000004	43
CGTAACG	50	2.1827873E-11	45.000004	1
CACCGTA	25	3.890861E-5	45.000004	25
TATCCCG	25	3.890861E-5	45.000004	1
TATCACG	45	3.8562575E-10	45.0	1
GATCGGT	20	7.0333545E-4	45.0	9
TAGCGAA	20	7.0333545E-4	45.0	32
TAACGGT	20	7.0333545E-4	45.0	22
CTAGTAT	20	7.0333545E-4	45.0	43
TTGACGT	20	7.0333545E-4	45.0	39
ACTACGT	20	7.0333545E-4	45.0	22
ACGTCGC	20	7.0333545E-4	45.0	45
TCATACG	45	3.8562575E-10	45.0	1
TATCGCG	20	7.0333545E-4	45.0	1
>>END_MODULE