FastQCFastQC Report
Sat 18 Jun 2016
SRR3553094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967419
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT35690.36891977519564945No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT15760.1629076956313655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15260.1577393042725024No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC14810.1530877520495256No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT14500.14988334940703046No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG12190.12600538132908284No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC10120.10460824110338954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG351.2116652E-745.0000049
AATTGCG302.165143E-645.0000041
TAGTGCG302.165143E-645.0000041
CCGTTAG253.8903454E-545.00000440
TGCGTAA253.8903454E-545.00000421
ATCTCGC207.0327334E-445.011
GGTACGT207.0327334E-445.08
ACGCATT207.0327334E-445.022
GCGCGAT207.0327334E-445.038
CGCGATA207.0327334E-445.045
CTTGCGA207.0327334E-445.037
ACTACCG207.0327334E-445.01
CGAATAT551.8189894E-1245.013
GACGCAT207.0327334E-445.016
GATTACC207.0327334E-445.021
TCGGTAC207.0327334E-445.042
CTTACCG900.042.51
AACGGGC3500.042.428574
TATACGG850.042.352942
CGATTGG750.042.0000042