##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	967419
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860767671505315	33.0	31.0	34.0	30.0	34.0
2	32.072782320793785	34.0	31.0	34.0	30.0	34.0
3	32.22247030500745	34.0	31.0	34.0	30.0	34.0
4	35.875508957339065	37.0	35.0	37.0	35.0	37.0
5	35.6503293815813	37.0	35.0	37.0	33.0	37.0
6	35.01477126250363	37.0	35.0	37.0	33.0	37.0
7	35.42730399134191	37.0	35.0	37.0	33.0	37.0
8	35.82086148814526	37.0	35.0	37.0	35.0	37.0
9	37.515604924029816	39.0	37.0	39.0	35.0	39.0
10	37.056867810121574	39.0	37.0	39.0	33.0	39.0
11	37.00707759512683	39.0	37.0	39.0	33.0	39.0
12	36.88742106574297	39.0	37.0	39.0	33.0	39.0
13	36.874105222245994	39.0	37.0	39.0	33.0	39.0
14	38.09111460494367	40.0	37.0	41.0	33.0	41.0
15	38.101544418705856	40.0	37.0	41.0	33.0	41.0
16	38.01129500247566	40.0	37.0	41.0	33.0	41.0
17	38.026827052187315	40.0	37.0	41.0	33.0	41.0
18	38.01114615280452	40.0	37.0	41.0	33.0	41.0
19	38.05283233014857	40.0	37.0	41.0	33.0	41.0
20	37.934982670383775	40.0	37.0	41.0	33.0	41.0
21	37.92961891383155	40.0	37.0	41.0	33.0	41.0
22	37.885993556049655	40.0	37.0	41.0	33.0	41.0
23	37.80456451651249	40.0	37.0	41.0	33.0	41.0
24	37.64485708881054	40.0	36.0	41.0	33.0	41.0
25	37.694521195056126	40.0	36.0	41.0	33.0	41.0
26	37.625583123755064	40.0	36.0	41.0	33.0	41.0
27	37.57493598947302	40.0	36.0	41.0	33.0	41.0
28	37.52026784671378	40.0	36.0	41.0	33.0	41.0
29	37.28304178437678	39.0	36.0	41.0	32.0	41.0
30	37.372013574263065	39.0	36.0	41.0	32.0	41.0
31	37.32208174534509	39.0	36.0	41.0	32.0	41.0
32	37.2885926366962	39.0	36.0	41.0	32.0	41.0
33	37.07741113209478	39.0	35.0	41.0	31.0	41.0
34	37.051201185835716	39.0	35.0	41.0	31.0	41.0
35	36.95545466855623	39.0	35.0	41.0	31.0	41.0
36	36.927734518342106	39.0	35.0	41.0	31.0	41.0
37	36.862555934915484	39.0	35.0	41.0	31.0	41.0
38	36.68537004131612	39.0	35.0	41.0	31.0	41.0
39	36.672258866116955	39.0	35.0	40.0	31.0	41.0
40	36.60379421946437	39.0	35.0	40.0	31.0	41.0
41	36.562050156137104	39.0	35.0	40.0	31.0	41.0
42	36.45806522303159	39.0	35.0	40.0	30.0	41.0
43	36.36133567771565	38.0	35.0	40.0	30.0	41.0
44	36.27442607598155	38.0	35.0	40.0	30.0	41.0
45	36.075740708007594	38.0	35.0	40.0	30.0	41.0
46	35.91805102029214	38.0	35.0	40.0	29.0	41.0
47	35.793778083746545	38.0	35.0	40.0	29.0	41.0
48	35.665388006644484	38.0	34.0	40.0	29.0	41.0
49	35.554353387725484	37.0	34.0	40.0	29.0	41.0
50	35.47964739166793	37.0	34.0	40.0	28.0	41.0
51	34.25843403943896	36.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	10.0
11	5.0
12	8.0
13	16.0
14	21.0
15	52.0
16	70.0
17	176.0
18	328.0
19	611.0
20	1038.0
21	1622.0
22	2218.0
23	2936.0
24	3960.0
25	4883.0
26	6182.0
27	7774.0
28	9872.0
29	12649.0
30	16487.0
31	21322.0
32	28731.0
33	39534.0
34	61330.0
35	75919.0
36	92493.0
37	135665.0
38	202386.0
39	239045.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.35508915991933	31.731132012085766	20.79926071330003	27.114518114694874
2	24.22497387378168	29.22901038743295	20.056666242858576	26.48934949592679
3	20.894875953438998	29.641034546561524	21.953775975042873	27.510313524956608
4	21.988507564974434	30.555736449253118	19.887143006287864	27.568612979484584
5	18.661820782928594	33.009275195132616	17.63320753468766	30.69569648725113
6	20.436129536426307	37.73876675980108	19.18951354066852	22.635590163104094
7	85.44002133511952	5.390528819467056	4.840818714538375	4.328631130875039
8	87.25019872464775	4.215236624461583	3.9044095681395543	4.630155082751114
9	80.2575719517603	7.256421467843819	7.469255824001802	5.016750756394075
10	38.45996409001684	30.75120501044532	13.588217721587025	17.200613177950817
11	28.508123160698723	29.338787019895207	22.92946489576905	19.22362492363702
12	26.841316947465373	23.933579968968978	28.725402333425333	20.49970075014032
13	25.503323792482885	24.977284919977798	27.051463740116745	22.467927547422576
14	21.960391515982217	26.385051358304935	28.31265459950652	23.34190252620633
15	21.106263160016496	28.370437214898615	27.51754927285902	23.00575035222587
16	22.754153060876416	26.849069534503663	26.995335009959494	23.40144239466043
17	22.163716032039893	26.54155024865131	27.979396724687028	23.31533699462177
18	23.98443693994019	26.329852938592275	26.105027914481727	23.580682206985802
19	24.410519123564868	28.561460959522194	25.428382117779368	21.59963779913357
20	25.65444755581604	27.570576968200953	24.832776697583984	21.942198778399018
21	25.94263705798625	26.856098546751717	25.179989229072408	22.021275166189625
22	24.27665778737031	25.15673146795752	25.17213327420694	25.394477470465226
23	23.87114580135391	27.907142613490123	23.836517579249527	24.38519400590644
24	22.013315843496976	27.682627692861107	26.5847580004114	23.719298463230512
25	24.09286979064914	28.241537534408568	23.401339026833252	24.26425364810904
26	25.14257007563424	27.437645942450995	23.865770674340695	23.55401330757407
27	23.348828170627208	25.97126994611435	25.45474091370957	25.22516096954887
28	24.08573741057391	27.281353787758977	26.13190354954782	22.5010052521193
29	23.37353308132257	29.072718232740936	23.762609582817788	23.79113910311871
30	25.42652149689018	25.596561572596777	25.387655193871527	23.589261736641518
31	25.593770641262992	27.10366449284126	22.449734809839377	24.852830056056373
32	25.19838870230996	26.228862571440086	24.769205483869968	23.80354324237998
33	24.119435322233695	26.689262873687614	22.864756635956084	26.3265451681226
34	23.704413496116988	26.18431103792669	25.78634490329423	24.324930562662097
35	23.920555622744644	26.469399505281576	25.357161684854233	24.252883187119544
36	24.725894364282695	28.358549914773228	23.78473029783372	23.13082542311036
37	23.79734117274935	28.393178136877612	22.853489542793763	24.955991147579283
38	23.74317643130846	26.515501556202636	25.52968258841309	24.211639424075816
39	22.28806752813414	24.88580439292592	25.921653389069267	26.90447468987068
40	23.706067381351822	25.619302494575773	25.267955250000256	25.406674874072145
41	21.676129991244743	25.896018167929302	26.601503588414122	25.82634825241183
42	24.313456733845417	26.832427314328122	24.815307534791025	24.038808417035433
43	23.531479121249426	25.195184299667467	26.012617076985258	25.26071950209785
44	24.842079802029936	23.565590504217923	25.18836202307377	26.403967670678373
45	24.94503415789849	24.025370599502388	25.91700183684629	25.112593405752833
46	22.060554940516983	24.73778166440808	28.076355746579303	25.12530764849564
47	23.347794492355433	26.30690528095892	25.28253011363225	25.062770113053396
48	23.0128827323011	25.887542006100766	26.700633334677114	24.398941926921015
49	22.799531537007233	25.919792768180077	26.279616174584124	25.00105952022857
50	23.116974134268606	24.529598860473072	26.092830510874816	26.26059649438351
51	22.633212703079018	23.817394531221737	27.291173731340816	26.258219034358433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2181.0
1	1770.0
2	1359.0
3	987.5
4	616.0
5	613.5
6	611.0
7	660.5
8	710.0
9	788.0
10	866.0
11	997.0
12	1128.0
13	1151.5
14	1175.0
15	1272.5
16	1370.0
17	1397.5
18	1425.0
19	1422.0
20	1419.0
21	1650.0
22	1881.0
23	3054.5
24	4228.0
25	4706.0
26	5965.0
27	6746.0
28	9038.5
29	11331.0
30	12772.0
31	14213.0
32	16554.0
33	18895.0
34	22323.0
35	25751.0
36	27245.0
37	28739.0
38	29927.5
39	31116.0
40	32977.5
41	34839.0
42	38571.5
43	42304.0
44	45882.5
45	49461.0
46	57243.5
47	65026.0
48	71923.0
49	78820.0
50	84107.0
51	89394.0
52	84307.5
53	79221.0
54	73657.5
55	68094.0
56	62895.0
57	57696.0
58	54720.0
59	51744.0
60	47330.0
61	42916.0
62	39715.5
63	36515.0
64	33622.0
65	30729.0
66	26511.0
67	22293.0
68	20085.5
69	17878.0
70	15489.5
71	13101.0
72	11218.5
73	9336.0
74	7817.5
75	5329.5
76	4360.0
77	3610.0
78	2860.0
79	2243.0
80	1626.0
81	1259.0
82	892.0
83	665.0
84	438.0
85	379.5
86	321.0
87	232.5
88	144.0
89	91.5
90	39.0
91	57.5
92	76.0
93	60.0
94	44.0
95	25.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	967419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.46860910040539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17278570277469	18.393735083497372
2	10.066325584478621	4.926179716080344
3	3.6651617197684034	2.690442282323479
4	1.785197961310327	1.7472524432857208
5	1.010273072387728	1.2359988446460437
6	0.6834610211859001	1.0034004337657008
7	0.49670774539179	0.8507623361394777
8	0.40375960234555985	0.7903548704262903
9	0.31108024189607325	0.6850530754033112
>10	4.400354236808616	27.152132611069373
>50	1.6995663052741048	29.10103612302317
>100	0.29972052321017556	9.757151114524385
>500	0.0025875153082892855	0.41786514409098013
>1k	0.0030187678596708327	1.2486359217243856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	3569	0.36891977519564945	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT	1576	0.1629076956313655	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1526	0.1577393042725024	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	1481	0.1530877520495256	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT	1450	0.14988334940703046	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1219	0.12600538132908284	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	1012	0.10460824110338954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0673565435452477E-4	0.0	0.0	0.4167790791787219	0.0
2	2.0673565435452477E-4	0.0	0.0	0.5935380636518406	0.0
3	2.0673565435452477E-4	0.0	0.0	0.8372794001358254	0.0
4	2.0673565435452477E-4	0.0	0.0	1.7931216980439706	0.0
5	2.0673565435452477E-4	0.0	0.0	1.956339497156868	0.0
6	2.0673565435452477E-4	0.0	0.0	2.8432354543377794	0.0
7	2.0673565435452477E-4	0.0	0.0	3.535283057289551	0.0
8	2.0673565435452477E-4	0.0	0.0	4.2161669349061786	0.0
9	2.0673565435452477E-4	0.0	0.0	5.089211603245336	0.0
10	2.0673565435452477E-4	0.0	0.0	6.305747561294537	0.0
11	2.0673565435452477E-4	0.0	0.0	7.4618133404450395	0.0
12	2.0673565435452477E-4	0.0	0.0	7.977722165886757	0.0
13	2.0673565435452477E-4	0.0	0.0	8.217122053629296	0.0
14	2.0673565435452477E-4	0.0	0.0	8.476161828535515	0.0
15	2.0673565435452477E-4	0.0	0.0	8.696645403904617	0.0
16	2.0673565435452477E-4	0.0	0.0	9.090476825449986	0.0
17	2.0673565435452477E-4	0.0	0.0	9.565141887847975	0.0
18	2.0673565435452477E-4	0.0	0.0	10.197546254518466	0.0
19	2.0673565435452477E-4	0.0	0.0	10.484805446244078	0.0
20	2.0673565435452477E-4	0.0	0.0	10.816926274964622	0.0
21	2.0673565435452477E-4	0.0	0.0	11.182951750999308	0.0
22	2.0673565435452477E-4	0.0	0.0	11.57481918382831	0.0
23	2.0673565435452477E-4	0.0	0.0	11.988910699500423	0.0
24	2.0673565435452477E-4	0.0	0.0	12.291261593993916	0.0
25	2.0673565435452477E-4	0.0	0.0	12.558880898555849	0.0
26	2.0673565435452477E-4	0.0	0.0	12.805413166373619	0.0
27	2.0673565435452477E-4	0.0	0.0	13.088744380666496	0.0
28	2.0673565435452477E-4	0.0	0.0	13.371662123650662	0.0
29	2.0673565435452477E-4	0.0	0.0	13.658611211894742	0.0
30	2.0673565435452477E-4	0.0	0.0	14.080041843296442	0.0
31	2.0673565435452477E-4	0.0	0.0	14.358308034057632	0.0
32	3.1010348153178715E-4	0.0	0.0	14.650838984969285	0.0
33	3.1010348153178715E-4	0.0	0.0	14.961666041291313	0.0
34	3.1010348153178715E-4	0.0	0.0	15.235900886792589	0.0
35	3.1010348153178715E-4	0.0	0.0	15.534117068198992	0.0
36	3.1010348153178715E-4	0.0	0.0	15.802770051032697	0.0
37	3.1010348153178715E-4	0.0	0.0	16.085481058362507	0.0
38	3.1010348153178715E-4	0.0	0.0	16.375221077940374	0.0
39	3.1010348153178715E-4	0.0	0.0	16.671266534976056	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATCG	35	1.2116652E-7	45.000004	9
AATTGCG	30	2.165143E-6	45.000004	1
TAGTGCG	30	2.165143E-6	45.000004	1
CCGTTAG	25	3.8903454E-5	45.000004	40
TGCGTAA	25	3.8903454E-5	45.000004	21
ATCTCGC	20	7.0327334E-4	45.0	11
GGTACGT	20	7.0327334E-4	45.0	8
ACGCATT	20	7.0327334E-4	45.0	22
GCGCGAT	20	7.0327334E-4	45.0	38
CGCGATA	20	7.0327334E-4	45.0	45
CTTGCGA	20	7.0327334E-4	45.0	37
ACTACCG	20	7.0327334E-4	45.0	1
CGAATAT	55	1.8189894E-12	45.0	13
GACGCAT	20	7.0327334E-4	45.0	16
GATTACC	20	7.0327334E-4	45.0	21
TCGGTAC	20	7.0327334E-4	45.0	42
CTTACCG	90	0.0	42.5	1
AACGGGC	350	0.0	42.42857	4
TATACGG	85	0.0	42.35294	2
CGATTGG	75	0.0	42.000004	2
>>END_MODULE