FastQCFastQC Report
Sat 18 Jun 2016
SRR3553093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974822
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT38140.3912509155517623No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC17380.1782889594202839No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT16920.17357014921698524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15780.16187570653924513No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT15080.15469490840379063No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG12220.12535621887893378No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC9960.10217249918446648No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT207.032758E-445.00000413
CGCGAAA207.032758E-445.00000414
ACGATAG207.032758E-445.0000041
CGAATCG207.032758E-445.00000419
CGAATAT351.2116652E-745.00000413
TACTCGA207.032758E-445.00000424
GTAACAC207.032758E-445.00000411
TCGCGAA207.032758E-445.00000416
GACTAAC351.2116652E-745.00000429
CGCATAG253.8903687E-545.01
CCGTATA253.8903687E-545.06
GTCCGAA253.8903687E-545.035
ACCGTAT253.8903687E-545.05
TCGACAA253.8903687E-545.044
TGTCGTA253.8903687E-545.028
TTACGGG3450.041.0869563
GTTTACG501.0804797E-940.511
CGATTAG501.0804797E-940.51
TCGTTGA2400.040.31250424
TTAATCG2400.040.31250420