##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	974822
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8736384693821	33.0	31.0	34.0	30.0	34.0
2	32.081702095356896	33.0	31.0	34.0	30.0	34.0
3	32.21607124172413	34.0	31.0	34.0	30.0	34.0
4	35.87171196382519	37.0	35.0	37.0	35.0	37.0
5	35.66220191994026	37.0	35.0	37.0	33.0	37.0
6	35.69109847746563	37.0	35.0	37.0	33.0	37.0
7	35.77782507986073	37.0	35.0	37.0	35.0	37.0
8	35.813551602241226	37.0	35.0	37.0	35.0	37.0
9	37.54150193573801	39.0	37.0	39.0	35.0	39.0
10	37.06959526970052	39.0	37.0	39.0	33.0	39.0
11	37.021682932884154	39.0	37.0	39.0	33.0	39.0
12	36.94266645602992	39.0	37.0	39.0	33.0	39.0
13	36.918744139955805	39.0	37.0	39.0	33.0	39.0
14	38.13234826460626	40.0	37.0	41.0	33.0	41.0
15	38.14271220797233	40.0	37.0	41.0	33.0	41.0
16	38.06079366284306	40.0	37.0	41.0	33.0	41.0
17	38.04648951295724	40.0	37.0	41.0	33.0	41.0
18	37.99191544712778	40.0	37.0	41.0	33.0	41.0
19	38.05400575694845	40.0	37.0	41.0	33.0	41.0
20	37.952864215210575	40.0	37.0	41.0	33.0	41.0
21	37.930830449046084	40.0	37.0	41.0	33.0	41.0
22	37.87123495366333	40.0	37.0	41.0	33.0	41.0
23	37.760530640465646	40.0	37.0	41.0	33.0	41.0
24	37.618814511777536	39.0	36.0	41.0	33.0	41.0
25	37.66977355865994	40.0	36.0	41.0	33.0	41.0
26	37.58231861816824	40.0	36.0	41.0	33.0	41.0
27	37.551455547782055	40.0	36.0	41.0	33.0	41.0
28	37.49363370953877	39.0	36.0	41.0	32.0	41.0
29	37.30907386168962	39.0	36.0	41.0	32.0	41.0
30	37.3769190683017	39.0	36.0	41.0	32.0	41.0
31	37.30525675456647	39.0	36.0	41.0	32.0	41.0
32	37.2523394014497	39.0	36.0	41.0	32.0	41.0
33	37.19637533826688	39.0	36.0	41.0	32.0	41.0
34	37.1052171575939	39.0	35.0	41.0	31.0	41.0
35	36.994251258178416	39.0	35.0	41.0	31.0	41.0
36	36.917241301488886	39.0	35.0	41.0	31.0	41.0
37	36.852496148014716	39.0	35.0	41.0	31.0	41.0
38	36.785823463155324	39.0	35.0	41.0	31.0	41.0
39	36.71945442347423	39.0	35.0	41.0	31.0	41.0
40	36.633226373635395	39.0	35.0	40.0	31.0	41.0
41	36.53330351592393	39.0	35.0	40.0	31.0	41.0
42	36.43364327025857	39.0	35.0	40.0	30.0	41.0
43	36.31331873921598	38.0	35.0	40.0	30.0	41.0
44	36.23621132883747	38.0	35.0	40.0	30.0	41.0
45	36.050705667291055	38.0	35.0	40.0	30.0	41.0
46	35.90088344333632	38.0	35.0	40.0	29.0	41.0
47	35.75673097242368	38.0	34.0	40.0	29.0	41.0
48	35.64855635182628	38.0	34.0	40.0	29.0	41.0
49	35.47180100572207	37.0	34.0	40.0	28.0	41.0
50	35.41489215467029	37.0	34.0	40.0	28.0	41.0
51	34.12516336315758	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	3.0
12	5.0
13	14.0
14	16.0
15	51.0
16	126.0
17	182.0
18	329.0
19	615.0
20	1015.0
21	1621.0
22	2209.0
23	2981.0
24	3904.0
25	4966.0
26	6093.0
27	7610.0
28	9845.0
29	12768.0
30	16318.0
31	21700.0
32	28420.0
33	39179.0
34	61724.0
35	76480.0
36	92001.0
37	135787.0
38	206148.0
39	242653.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.362178941386222	31.857303179452252	20.736708855565425	27.043809023596104
2	24.13763743534717	29.22923364470642	20.147370494305626	26.485758425640782
3	20.929769742578642	29.61638124703792	21.975293951100817	27.478555059282616
4	21.930875585491506	30.593174959120745	19.849777703006293	27.626171752381463
5	18.606781545759123	33.117841000716034	17.653479301862287	30.621898151662556
6	20.853140368190296	36.55826397024277	19.46047586123415	23.128119800332776
7	85.2298163151837	5.416168285081789	4.970651052192093	4.3833643475424235
8	86.79256315511961	4.346331945729579	4.028632919650972	4.832471979499847
9	79.96977909813279	7.304102697723276	7.684172084749831	5.041946119394105
10	38.29796619280238	30.8121893022521	13.636643407719562	17.253201097225958
11	28.394722318536104	29.333662966162027	23.035795252876937	19.235819462424935
12	26.76663021556756	23.926214221673288	28.717960817462057	20.58919474529709
13	25.418178908559717	25.001282285381333	27.110590446255827	22.469948359803123
14	21.966061496355234	26.377123208134407	28.384566618315958	23.2722486771944
15	20.994396925797734	28.471556858585465	27.5620574833149	22.9719887323019
16	22.582173976377227	26.918965718869703	27.14310920352639	23.355751101226684
17	22.195231539706736	26.59829179070641	27.956488466612367	23.249988202974492
18	23.787727400489526	26.406154149167744	26.12005063488514	23.68606781545759
19	24.31992712515721	28.558341933193958	25.57420739376009	21.54752354788874
20	25.45633972150813	27.74198776802329	24.906495749993333	21.895176760475245
21	25.703564343028777	26.990671117393738	25.115456975735057	22.190307563842424
22	24.233347216209726	25.259483269766175	25.17762217102199	25.32954734300211
23	23.77828978008293	28.0186536618993	23.791830713709786	24.41122584430799
24	21.818855134578417	27.752451216734954	26.597676293723367	23.831017354963265
25	24.03874758673891	28.223101243098743	23.385294956412555	24.352856213749792
26	25.09258100453211	27.52461475018003	23.84199371782746	23.540810527460398
27	23.378216741107607	26.05408987486946	25.4084335396616	25.15925984436133
28	23.992687895841495	27.344171551319114	26.12323070263084	22.539909850208552
29	23.2801475551434	29.18717468419876	23.71827882423663	23.814398936421213
30	25.577695209997316	25.606520985369635	25.270151884138848	23.545631920494202
31	25.548972017455498	27.165164512085283	22.35485042397484	24.931013046484384
32	25.1002747168201	26.312393442084815	24.802681925520762	23.78464991557433
33	24.146664724431744	26.58762317633373	22.879253853524027	26.386458245710497
34	23.529526416104684	26.331576431389525	25.789631337823728	24.349265814682063
35	23.964375034621703	26.28756839710224	25.32616210959539	24.42189445868066
36	24.78493509584314	28.361793229943515	23.728229358795762	23.125042315417584
37	23.694377024728617	28.36753786845188	22.73840762723861	25.199677479580888
38	23.662473764441096	26.45498357648884	25.541586053659028	24.34095660541104
39	22.23636725473984	24.791705562656567	26.025674430819162	26.946252751784428
40	23.73171717503298	25.618318010877882	25.21865530322459	25.431309510864548
41	21.502079353974366	25.826971488128088	26.642299824993692	26.028649332903854
42	24.33952044578395	26.791660426211145	24.85079327302831	24.018025854976603
43	23.493212094105385	25.21455199000433	25.983102556158972	25.309133359731316
44	24.850382941706282	23.494956002223997	25.10766068061656	26.547000375453162
45	24.97614949190724	23.983455441095913	25.921347692194065	25.119047374802783
46	22.022379470303296	24.556380549474675	28.076715543965975	25.344524436256055
47	23.15961272929827	26.377123208134407	25.360527357815066	25.102736704752253
48	23.042873468181885	25.583337265675173	26.842644092972872	24.531145173170078
49	22.76159134693308	25.853745606890282	26.278233359526148	25.106429686650483
50	23.03733399533453	24.53391490959375	26.15164614668114	26.27710494839058
51	22.50923758388711	23.630057590001048	27.445831136351046	26.4148736897608
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2231.0
1	1835.0
2	1439.0
3	1017.0
4	595.0
5	609.0
6	623.0
7	677.5
8	732.0
9	810.0
10	888.0
11	1003.0
12	1118.0
13	1143.5
14	1169.0
15	1309.5
16	1450.0
17	1461.5
18	1473.0
19	1467.5
20	1462.0
21	1693.5
22	1925.0
23	3052.0
24	4179.0
25	4761.5
26	6179.5
27	7015.0
28	9262.5
29	11510.0
30	12945.5
31	14381.0
32	16734.0
33	19087.0
34	22350.5
35	25614.0
36	27403.0
37	29192.0
38	30128.5
39	31065.0
40	32948.0
41	34831.0
42	38805.0
43	42779.0
44	45921.5
45	49064.0
46	57299.0
47	65534.0
48	72479.0
49	79424.0
50	84967.0
51	90510.0
52	85361.5
53	80213.0
54	74661.5
55	69110.0
56	63523.0
57	57936.0
58	55008.5
59	52081.0
60	47833.5
61	43586.0
62	40152.5
63	36719.0
64	33644.0
65	30569.0
66	26562.5
67	22556.0
68	20208.5
69	17861.0
70	15314.5
71	12768.0
72	11092.5
73	9417.0
74	7880.0
75	5412.5
76	4482.0
77	3698.0
78	2914.0
79	2256.5
80	1599.0
81	1290.5
82	982.0
83	725.5
84	469.0
85	378.0
86	287.0
87	212.0
88	137.0
89	94.5
90	52.0
91	56.5
92	61.0
93	49.0
94	37.0
95	22.5
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	974822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.620087585242132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26382110109995	18.530018675090755
2	10.107366175820156	4.976884810096121
3	3.653557230295497	2.698526970227094
4	1.7769014693629255	1.7498987922424265
5	1.0212974256346694	1.2572216034853936
6	0.6642196467187159	0.9811887526832024
7	0.49632991204576216	0.8553780134019531
8	0.39271079484705	0.7734859331843541
9	0.3115256931857959	0.6902810866158807
>10	4.328160562447423	26.76741868452438
>50	1.6726630642714961	28.892915591956992
>100	0.30599030699896984	10.10709508832375
>500	0.0029381785308543054	0.5258988068430694
>1k	0.002518438740732262	1.1937871913246487
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	3814	0.3912509155517623	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	1738	0.1782889594202839	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT	1692	0.17357014921698524	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1578	0.16187570653924513	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGT	1508	0.15469490840379063	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1222	0.12535621887893378	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	996	0.10217249918446648	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0258283050649246E-4	0.0	0.0	0.44787663799134614	0.0
2	1.0258283050649246E-4	0.0	0.0	0.6421685189706429	0.0
3	1.0258283050649246E-4	0.0	0.0	0.8944196991861079	0.0
4	1.0258283050649246E-4	0.0	0.0	1.9313269499457337	0.0
5	1.0258283050649246E-4	0.0	0.0	2.1152579650438748	0.0
6	1.0258283050649246E-4	0.0	0.0	3.054608943991826	0.0
7	1.0258283050649246E-4	0.0	0.0	3.784896114367546	0.0
8	1.0258283050649246E-4	0.0	0.0	4.507284406794266	0.0
9	1.0258283050649246E-4	0.0	0.0	5.412577886014062	0.0
10	1.0258283050649246E-4	0.0	0.0	6.657420534210348	0.0
11	1.0258283050649246E-4	0.0	0.0	7.874770983830894	0.0
12	1.0258283050649246E-4	0.0	0.0	8.418767734006824	0.0
13	1.0258283050649246E-4	0.0	0.0	8.665684607035951	0.0
14	1.0258283050649246E-4	0.0	0.0	8.941940169589936	0.0
15	1.0258283050649246E-4	0.0	0.0	9.165468157263582	0.0
16	1.0258283050649246E-4	0.0	0.0	9.574158154001449	0.0
17	1.0258283050649246E-4	0.0	0.0	10.048706327924483	0.0
18	1.0258283050649246E-4	0.0	0.0	10.722572941521632	0.0
19	1.0258283050649246E-4	0.0	0.0	11.02826977643098	0.0
20	1.0258283050649246E-4	0.0	0.0	11.371819675797223	0.0
21	1.0258283050649246E-4	0.0	0.0	11.745323761671361	0.0
22	1.0258283050649246E-4	0.0	0.0	12.136574677223123	0.0
23	1.0258283050649246E-4	0.0	0.0	12.553676466062521	0.0
24	1.0258283050649246E-4	0.0	0.0	12.860912043429467	0.0
25	1.0258283050649246E-4	0.0	0.0	13.13870634844105	0.0
26	1.0258283050649246E-4	0.0	0.0	13.379673417300799	0.0
27	1.0258283050649246E-4	0.0	0.0	13.670803490278225	0.0
28	1.0258283050649246E-4	0.0	0.0	13.946135807357651	0.0
29	1.0258283050649246E-4	0.0	0.0	14.24742158055522	0.0
30	1.0258283050649246E-4	0.0	0.0	14.667703437140318	0.0
31	1.0258283050649246E-4	0.0	0.0	14.950729466507731	0.0
32	1.0258283050649246E-4	0.0	0.0	15.262068357094936	0.0
33	1.0258283050649246E-4	0.0	0.0	15.56520062124162	0.0
34	1.0258283050649246E-4	0.0	0.0	15.851304135524229	0.0
35	2.0516566101298493E-4	0.0	0.0	16.17208064651803	0.0
36	2.0516566101298493E-4	0.0	0.0	16.454286013241394	0.0
37	2.0516566101298493E-4	0.0	0.0	16.740286944693494	0.0
38	2.0516566101298493E-4	0.0	0.0	17.03234026314548	0.0
39	2.0516566101298493E-4	0.0	0.0	17.337421601071785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCT	20	7.032758E-4	45.000004	13
CGCGAAA	20	7.032758E-4	45.000004	14
ACGATAG	20	7.032758E-4	45.000004	1
CGAATCG	20	7.032758E-4	45.000004	19
CGAATAT	35	1.2116652E-7	45.000004	13
TACTCGA	20	7.032758E-4	45.000004	24
GTAACAC	20	7.032758E-4	45.000004	11
TCGCGAA	20	7.032758E-4	45.000004	16
GACTAAC	35	1.2116652E-7	45.000004	29
CGCATAG	25	3.8903687E-5	45.0	1
CCGTATA	25	3.8903687E-5	45.0	6
GTCCGAA	25	3.8903687E-5	45.0	35
ACCGTAT	25	3.8903687E-5	45.0	5
TCGACAA	25	3.8903687E-5	45.0	44
TGTCGTA	25	3.8903687E-5	45.0	28
TTACGGG	345	0.0	41.086956	3
GTTTACG	50	1.0804797E-9	40.5	11
CGATTAG	50	1.0804797E-9	40.5	1
TCGTTGA	240	0.0	40.312504	24
TTAATCG	240	0.0	40.312504	20
>>END_MODULE