FastQCFastQC Report
Sat 18 Jun 2016
SRR3553090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930550
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT46930.5043253989576058Illumina Single End Adapter 2 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC21340.22932674224920746No Hit
AATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT20080.21578636290365913No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGT19420.20869378324646715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14160.15216807264520982No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC11950.1284186771264306No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG11390.12240073075063135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT207.0325925E-445.00000439
TTTCGAT207.0325925E-445.00000421
ATATGCG406.8121153E-945.0000041
TCTAGCG502.1827873E-1145.0000041
CGTTAGT207.0325925E-445.0000046
ATTCCCG207.0325925E-445.0000041
CCCGTAT207.0325925E-445.00000417
CCCCGTA207.0325925E-445.00000416
TCTACCG800.045.0000041
ATATACG253.8902304E-545.0000041
TCGTAAT207.0325925E-445.00000439
TTACGAA253.8902304E-545.00000415
AACTCGC207.0325925E-445.00000431
TATCGCG207.0325925E-445.0000041
AATTGCG453.8380676E-1045.01
CGTAGCG453.8380676E-1045.01
CATAACG453.8380676E-1045.01
TATAGCG351.2115925E-745.01
CAATGCG351.2115925E-745.01
CGACTTA351.2115925E-745.017