##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	930550
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816305410778572	33.0	31.0	34.0	30.0	34.0
2	32.018568588469186	33.0	31.0	34.0	30.0	34.0
3	32.172197087743804	34.0	31.0	34.0	30.0	34.0
4	35.83645263553812	37.0	35.0	37.0	35.0	37.0
5	35.60807264520982	37.0	35.0	37.0	33.0	37.0
6	34.940332061683954	37.0	35.0	37.0	33.0	37.0
7	35.363049809252594	37.0	35.0	37.0	33.0	37.0
8	35.77116006662726	37.0	35.0	37.0	35.0	37.0
9	37.41473752082102	39.0	37.0	39.0	35.0	39.0
10	36.983259362742466	39.0	37.0	39.0	33.0	39.0
11	36.988954919133846	39.0	37.0	39.0	33.0	39.0
12	36.865280747944766	39.0	37.0	39.0	33.0	39.0
13	36.853489871581324	39.0	37.0	39.0	33.0	39.0
14	38.03850840900542	40.0	37.0	41.0	33.0	41.0
15	38.05015958304229	40.0	37.0	41.0	33.0	41.0
16	37.94188705604213	40.0	37.0	41.0	33.0	41.0
17	37.969702863897695	40.0	37.0	41.0	33.0	41.0
18	37.90072645209822	40.0	37.0	41.0	33.0	41.0
19	37.95239159636774	40.0	37.0	41.0	33.0	41.0
20	37.866409112890224	40.0	37.0	41.0	33.0	41.0
21	37.85584331846757	40.0	37.0	41.0	33.0	41.0
22	37.81116651442695	40.0	37.0	41.0	33.0	41.0
23	37.73811294395787	40.0	36.0	41.0	33.0	41.0
24	37.5747385954543	39.0	36.0	41.0	32.0	41.0
25	37.60407930793617	39.0	36.0	41.0	32.0	41.0
26	37.56641126215679	39.0	36.0	41.0	33.0	41.0
27	37.48497017892644	39.0	36.0	41.0	32.0	41.0
28	37.4176089409489	39.0	36.0	41.0	32.0	41.0
29	37.23578528827038	39.0	36.0	41.0	32.0	41.0
30	37.26377948525066	39.0	36.0	41.0	32.0	41.0
31	37.206377948525066	39.0	36.0	41.0	32.0	41.0
32	37.18834130353017	39.0	36.0	41.0	32.0	41.0
33	36.93983450647467	39.0	35.0	41.0	31.0	41.0
34	36.969956477352106	39.0	35.0	41.0	31.0	41.0
35	36.89054860029015	39.0	35.0	41.0	31.0	41.0
36	36.89633764977702	39.0	35.0	41.0	31.0	41.0
37	36.855734780506154	39.0	35.0	41.0	31.0	41.0
38	36.70536134544087	39.0	35.0	41.0	31.0	41.0
39	36.656517113535	39.0	35.0	41.0	31.0	41.0
40	36.60306055558541	39.0	35.0	40.0	31.0	41.0
41	36.57868787276342	39.0	35.0	40.0	31.0	41.0
42	36.51790876363441	39.0	35.0	40.0	30.0	41.0
43	36.47012841867713	39.0	35.0	40.0	30.0	41.0
44	36.40044597281178	39.0	35.0	40.0	30.0	41.0
45	36.22321422814465	38.0	35.0	40.0	30.0	41.0
46	36.10957498253721	38.0	35.0	40.0	30.0	41.0
47	35.981988071570576	38.0	35.0	40.0	30.0	41.0
48	35.895962602761806	38.0	35.0	40.0	29.0	41.0
49	35.794748267153835	38.0	35.0	40.0	29.0	41.0
50	35.72199451937026	38.0	34.0	40.0	29.0	41.0
51	34.49904035247972	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	5.0
11	9.0
12	17.0
13	15.0
14	30.0
15	55.0
16	96.0
17	183.0
18	280.0
19	582.0
20	1052.0
21	1659.0
22	2311.0
23	3140.0
24	3961.0
25	5029.0
26	6083.0
27	7317.0
28	9487.0
29	12092.0
30	16202.0
31	21239.0
32	28140.0
33	38630.0
34	59097.0
35	72623.0
36	87141.0
37	125660.0
38	190403.0
39	237931.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.91413680081672	31.783891247111924	22.208693783246467	26.09327816882489
2	24.410187523507602	30.522594164741285	20.231476008811995	24.835742302939124
3	21.03992262640374	30.45553704798238	21.670839825909407	26.833700499704477
4	21.385954543012197	30.75922841330396	19.43710708720649	28.41770995647735
5	18.51732846166246	33.46676696577293	18.76943742947719	29.246467143087422
6	20.86475740153673	37.99215517704583	18.560958572887003	22.582128848530438
7	84.39901133738111	5.886303798828649	5.328784052442105	4.385900811348128
8	85.61968728171512	4.154854655848691	5.094191607114072	5.1312664553221214
9	78.50368061898877	8.442426521949384	8.082101982698404	4.971790876363441
10	35.774971790876364	33.82934823491483	13.346408038256946	17.049271935951857
11	27.722529686744394	28.148084466175916	25.14308742141744	18.986298425662245
12	28.277792703240017	23.39885014238891	28.716135618720113	19.60722153565096
13	26.691956369888775	24.321207887808285	26.61264843372199	22.374187308580947
14	23.027456880339585	25.919187577239267	28.907957659448712	22.145397882972436
15	20.75052388372468	29.112675299554024	27.860512600075225	22.27628821664607
16	22.468110257374672	26.91881145559078	28.0060179463758	22.607060340658748
17	23.227446134006772	26.40083821395949	27.184460802751058	23.18725484928268
18	22.772016549352532	26.93944441459352	26.924829401966576	23.363709634087368
19	23.21573263123959	30.431787652463598	25.968083391542635	20.384396324754178
20	24.904948686260813	28.197087743807426	25.1024662833808	21.79549728655096
21	25.343076675084625	27.603567782494228	25.675138359034978	21.37821718338617
22	23.54048680887647	27.166729353608083	25.48847455805706	23.804309279458387
23	23.59701251947773	27.512009026919564	24.710762452313148	24.18021600128956
24	21.734458116167858	28.173015958304227	25.904250174627908	24.188275750900004
25	23.323303422707	27.800333136317228	24.331739293965935	24.544624147009834
26	23.80312718284885	27.899736714846057	24.164311428724947	24.132824673580142
27	21.022621030573315	26.800924184621998	25.656547203266882	26.5199075815378
28	22.412981570039225	28.787706195260863	26.03600021492666	22.763312019773252
29	22.6797055504809	28.318413841276662	24.457793777873302	24.544086830369135
30	23.232496910429315	27.661383053033152	24.687872763419485	24.418247273118048
31	23.749288055451075	29.036483799903284	23.035194239965612	24.17903390468003
32	24.077373596260276	26.912578582558698	24.715598302079417	24.294449519101608
33	22.155391972489387	29.942077266132934	24.13895008328408	23.7635806780936
34	21.69426683144377	28.330342270700125	25.58809306324217	24.38729783461394
35	21.22110579764655	29.192198162377085	25.607221535650957	23.9794745043254
36	22.436945892214283	30.477352103594647	24.200204180323464	22.885497823867606
37	22.426521949384775	31.258932889151573	23.08000644779969	23.234538713663962
38	23.28493901456128	29.7581000483585	24.982644672505508	21.974316264574714
39	22.60125732093923	28.157756165708452	25.399494922357746	23.841491590994572
40	23.21218634141099	28.627155983020796	25.394873999247757	22.765783676320456
41	22.084896029230023	26.970286389769495	27.043361452904197	23.90145612809629
42	22.370103702111656	28.80457793777873	24.23910590510988	24.586212454999732
43	20.814679490623824	27.202944495191016	26.36859921551771	25.613776798667452
44	22.632851539412176	25.209392294879372	25.538230078985546	26.619526086722907
45	23.64225458062436	26.24834775132986	26.606415560689918	23.502982107355866
46	20.727634194831012	26.36763204556445	28.58341840846811	24.321315351136423
47	22.122400730750634	27.92767718016227	26.001611949922086	23.94831013916501
48	22.272312073504917	25.129332115415615	28.432969749073127	24.16538606200634
49	22.241685024985223	25.978184944387728	27.073773574767614	24.70635645585944
50	21.93036376336575	25.158884530653914	26.650797915211434	26.259953790768897
51	21.663102466283384	24.943205631078396	28.44779968835635	24.945892214281876
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1870.0
1	1629.0
2	1388.0
3	972.5
4	557.0
5	583.5
6	610.0
7	674.0
8	738.0
9	881.5
10	1025.0
11	1119.0
12	1213.0
13	1222.0
14	1231.0
15	1439.5
16	1648.0
17	1843.0
18	2038.0
19	2339.0
20	2640.0
21	2567.5
22	2495.0
23	3077.5
24	3660.0
25	4299.0
26	6568.0
27	8198.0
28	9719.0
29	11240.0
30	12912.0
31	14584.0
32	17209.5
33	19835.0
34	21317.0
35	22799.0
36	26766.5
37	30734.0
38	31843.0
39	32952.0
40	36929.0
41	40906.0
42	50745.0
43	60584.0
44	65959.0
45	71334.0
46	79038.5
47	86743.0
48	82568.0
49	78393.0
50	73855.5
51	69318.0
52	64953.5
53	60589.0
54	55134.0
55	49679.0
56	46838.0
57	43997.0
58	43390.0
59	42783.0
60	41174.0
61	39565.0
62	36322.5
63	33080.0
64	29470.0
65	25860.0
66	21796.0
67	17732.0
68	16115.5
69	14499.0
70	11961.0
71	9423.0
72	8247.5
73	7072.0
74	5810.5
75	3656.0
76	2763.0
77	2549.0
78	2335.0
79	1852.0
80	1369.0
81	1123.0
82	877.0
83	622.0
84	367.0
85	266.0
86	165.0
87	111.5
88	58.0
89	49.0
90	40.0
91	37.5
92	35.0
93	33.0
94	31.0
95	17.5
96	4.0
97	4.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	930550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.769466967878547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.10618680136659	20.105525866624983
2	9.970357381266592	5.338023051515201
3	3.486238677279278	2.799742533407047
4	1.7652249170270837	1.8901652042893065
5	1.0519653419415007	1.4080275736228034
6	0.7333879036267038	1.1779441956466048
7	0.5591013810996641	1.0476792167129914
8	0.45036673199667226	0.9644861884493063
9	0.40335332398860274	0.9717798134607223
>10	4.988894421103218	31.54586512565726
>50	1.3011536284645127	23.763062643566847
>100	0.17679609498184495	6.731797112718669
>500	0.004101997563383873	0.6607164705526178
>1k	0.0028713982943687115	1.5951850037756157
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	4693	0.5043253989576058	Illumina Single End Adapter 2 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC	2134	0.22932674224920746	No Hit
AATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	2008	0.21578636290365913	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGT	1942	0.20869378324646715	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1416	0.15216807264520982	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC	1195	0.1284186771264306	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1139	0.12240073075063135	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0746332813927248E-4	0.0	0.0	0.561603352855838	0.0
2	1.0746332813927248E-4	0.0	0.0	0.7891032185266778	0.0
3	1.0746332813927248E-4	0.0	0.0	1.1011767234431251	0.0
4	1.0746332813927248E-4	0.0	0.0	2.328515394121756	0.0
5	1.0746332813927248E-4	0.0	0.0	2.536349470743109	0.0
6	1.0746332813927248E-4	0.0	0.0	3.665466659502445	0.0
7	1.0746332813927248E-4	0.0	0.0	4.481113320079523	0.0
8	1.0746332813927248E-4	0.0	0.0	5.187899629251518	0.0
9	1.0746332813927248E-4	0.0	0.0	6.265756810488421	0.0
10	1.0746332813927248E-4	0.0	0.0	7.579281070334749	0.0
11	1.0746332813927248E-4	0.0	0.0	9.013056794368921	0.0
12	1.0746332813927248E-4	0.0	0.0	9.619794745043254	0.0
13	1.0746332813927248E-4	0.0	0.0	9.968513244855194	0.0
14	1.0746332813927248E-4	0.0	0.0	10.271559830207941	0.0
15	1.0746332813927248E-4	0.0	0.0	10.521089678147332	0.0
16	1.0746332813927248E-4	0.0	0.0	11.035194239965612	0.0
17	1.0746332813927248E-4	0.0	0.0	11.619149965074419	0.0
18	1.0746332813927248E-4	0.0	0.0	12.418247273118048	0.0
19	1.0746332813927248E-4	0.0	0.0	12.769007576164634	0.0
20	1.0746332813927248E-4	0.0	0.0	13.230777497179087	0.0
21	1.0746332813927248E-4	0.0	0.0	13.676857772285208	0.0
22	1.0746332813927248E-4	0.0	0.0	14.135081403471066	0.0
23	1.0746332813927248E-4	0.0	0.0	14.641233679007039	0.0
24	1.0746332813927248E-4	0.0	0.0	14.985761109021546	0.0
25	1.0746332813927248E-4	0.0	0.0	15.284401697920584	0.0
26	1.0746332813927248E-4	0.0	0.0	15.57466014722476	0.0
27	1.0746332813927248E-4	0.0	0.0	15.888882918703992	0.0
28	1.0746332813927248E-4	0.0	0.0	16.20847885659019	0.0
29	1.0746332813927248E-4	0.0	0.0	16.522271774756863	0.0
30	1.0746332813927248E-4	0.0	0.0	17.001020901617323	0.0
31	1.0746332813927248E-4	0.0	0.0	17.323088496050723	0.0
32	1.0746332813927248E-4	0.0	0.0	17.646123260437374	0.0
33	1.0746332813927248E-4	0.0	0.0	17.975283434527967	0.0
34	1.0746332813927248E-4	0.0	0.0	18.272312073504917	0.0
35	1.0746332813927248E-4	0.0	0.0	18.600505077642254	0.0
36	1.0746332813927248E-4	0.0	0.0	18.91891891891892	0.0
37	1.0746332813927248E-4	0.0	0.0	19.263553812261566	0.0
38	1.0746332813927248E-4	0.0	0.0	19.605931975713286	0.0
39	1.0746332813927248E-4	0.0	0.0	19.94702057922734	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.0325925E-4	45.000004	39
TTTCGAT	20	7.0325925E-4	45.000004	21
ATATGCG	40	6.8121153E-9	45.000004	1
TCTAGCG	50	2.1827873E-11	45.000004	1
CGTTAGT	20	7.0325925E-4	45.000004	6
ATTCCCG	20	7.0325925E-4	45.000004	1
CCCGTAT	20	7.0325925E-4	45.000004	17
CCCCGTA	20	7.0325925E-4	45.000004	16
TCTACCG	80	0.0	45.000004	1
ATATACG	25	3.8902304E-5	45.000004	1
TCGTAAT	20	7.0325925E-4	45.000004	39
TTACGAA	25	3.8902304E-5	45.000004	15
AACTCGC	20	7.0325925E-4	45.000004	31
TATCGCG	20	7.0325925E-4	45.000004	1
AATTGCG	45	3.8380676E-10	45.0	1
CGTAGCG	45	3.8380676E-10	45.0	1
CATAACG	45	3.8380676E-10	45.0	1
TATAGCG	35	1.2115925E-7	45.0	1
CAATGCG	35	1.2115925E-7	45.0	1
CGACTTA	35	1.2115925E-7	45.0	17
>>END_MODULE