SRR3553089_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	943037
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	5136	0.5446233816912804	Illumina Single End Adapter 2 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC	2377	0.25205797863710544	No Hit
AATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	2377	0.25205797863710544	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGT	2192	0.2324405086969016	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1468	0.1556672749849688	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC	1326	0.14060954130113665	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1121	0.1188712637998297	No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	975	0.10338936860377695	Illumina Single End Adapter 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTAG	45	3.8380676E-10	45.000004	1
TAACGAA	20	7.032642E-4	45.0	45
TATGCGA	20	7.032642E-4	45.0	16
ATCCGTG	20	7.032642E-4	45.0	11
TGCGCGT	25	3.8902712E-5	45.0	37
CGTAGCA	25	3.8902712E-5	45.0	29
TATAGCG	20	7.032642E-4	45.0	1
CACGTAA	20	7.032642E-4	45.0	34
TCGACGT	20	7.032642E-4	45.0	12
TATCGCG	20	7.032642E-4	45.0	1
TTACGTA	30	2.1650867E-6	44.999996	45
ATTCACG	80	0.0	42.1875	1
TACGAAT	120	0.0	41.249996	11
ATTACCG	55	6.002665E-11	40.909092	1
ACTACCG	55	6.002665E-11	40.909092	1
TCGGGTC	50	1.0804797E-9	40.5	5
CATAACG	50	1.0804797E-9	40.5	1
CGAGATA	350	0.0	40.5	19
CCCGATC	95	0.0	40.263157	41
TCGATAG	45	1.927583E-8	40.000004	1