##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553089_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 943037 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82616270623528 33.0 31.0 34.0 30.0 34.0 2 32.03066581692977 33.0 31.0 34.0 30.0 34.0 3 32.16961900752568 34.0 31.0 34.0 30.0 34.0 4 35.836343642932356 37.0 35.0 37.0 35.0 37.0 5 35.62287163706196 37.0 35.0 37.0 33.0 37.0 6 35.651262887882446 37.0 35.0 37.0 33.0 37.0 7 35.73089284937919 37.0 35.0 37.0 35.0 37.0 8 35.77370665201896 37.0 35.0 37.0 35.0 37.0 9 37.446313347196345 39.0 37.0 39.0 35.0 39.0 10 37.002811130422245 39.0 37.0 39.0 33.0 39.0 11 37.01018941992732 39.0 37.0 39.0 33.0 39.0 12 36.9144847975212 39.0 37.0 39.0 33.0 39.0 13 36.89965505065019 39.0 37.0 39.0 33.0 39.0 14 38.079852646290654 40.0 37.0 41.0 33.0 41.0 15 38.09278745160582 40.0 37.0 41.0 33.0 41.0 16 37.991572971155954 40.0 37.0 41.0 33.0 41.0 17 37.976357237308825 40.0 37.0 41.0 33.0 41.0 18 37.88562484822971 40.0 37.0 41.0 33.0 41.0 19 37.95728269410426 40.0 37.0 41.0 33.0 41.0 20 37.88026132590768 40.0 37.0 41.0 33.0 41.0 21 37.86321639553909 40.0 37.0 41.0 33.0 41.0 22 37.81505391623022 40.0 37.0 41.0 33.0 41.0 23 37.70177522196902 40.0 36.0 41.0 33.0 41.0 24 37.570158965130744 39.0 36.0 41.0 32.0 41.0 25 37.597188657497 39.0 36.0 41.0 32.0 41.0 26 37.54366689748122 39.0 36.0 41.0 33.0 41.0 27 37.49913524071696 39.0 36.0 41.0 32.0 41.0 28 37.407078407315936 39.0 36.0 41.0 32.0 41.0 29 37.28422002530123 39.0 36.0 41.0 32.0 41.0 30 37.29239043643038 39.0 36.0 41.0 32.0 41.0 31 37.20984860615225 39.0 36.0 41.0 32.0 41.0 32 37.18020077685181 39.0 36.0 41.0 32.0 41.0 33 37.07279141751596 39.0 35.0 41.0 31.0 41.0 34 37.05912069197709 39.0 35.0 41.0 31.0 41.0 35 36.947200374958776 39.0 35.0 41.0 31.0 41.0 36 36.92434549227655 39.0 35.0 41.0 31.0 41.0 37 36.880004708192786 39.0 35.0 41.0 31.0 41.0 38 36.82460921469677 39.0 35.0 41.0 31.0 41.0 39 36.73503690735358 39.0 35.0 41.0 31.0 41.0 40 36.6482831532591 39.0 35.0 40.0 31.0 41.0 41 36.57756800634546 39.0 35.0 40.0 31.0 41.0 42 36.51487905564681 39.0 35.0 40.0 30.0 41.0 43 36.45788765446107 39.0 35.0 40.0 30.0 41.0 44 36.402037247743195 39.0 35.0 40.0 30.0 41.0 45 36.24540288451036 38.0 35.0 40.0 30.0 41.0 46 36.127952561776475 38.0 35.0 40.0 30.0 41.0 47 35.98535688419437 38.0 35.0 40.0 30.0 41.0 48 35.91787915002275 38.0 35.0 40.0 29.0 41.0 49 35.74946900280689 38.0 35.0 40.0 29.0 41.0 50 35.69069506286604 38.0 34.0 40.0 29.0 41.0 51 34.39281809727508 37.0 33.0 40.0 25.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 7.0 11 11.0 12 12.0 13 17.0 14 49.0 15 56.0 16 82.0 17 163.0 18 316.0 19 573.0 20 1008.0 21 1568.0 22 2314.0 23 3048.0 24 3894.0 25 4973.0 26 5915.0 27 7425.0 28 9457.0 29 12423.0 30 15997.0 31 21180.0 32 28075.0 33 38203.0 34 59257.0 35 72551.0 36 87581.0 37 126442.0 38 197461.0 39 242911.0 40 64.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.831459423119135 31.89471887105172 22.16424169995451 26.109580005874637 2 24.192476011015472 30.681298824966568 20.34077135891805 24.785453805099905 3 21.037880804252644 30.454478456306592 21.70296605541458 26.804674684026185 4 21.41358186370206 30.616826275109037 19.3957395096905 28.573852351498402 5 18.448798933657958 33.47843191730547 18.867022184707494 29.205746964329077 6 21.314115989086325 36.564949201356896 19.036262628083524 23.084672181473262 7 84.1121822367521 5.878454397865619 5.523749333271123 4.4856140321111475 8 85.17969072263337 4.214468785424113 5.214641631240344 5.391198860702178 9 78.30530509407372 8.340605935928282 8.3296837769886 5.024405193009394 10 35.65681940369254 33.77375437018908 13.471051506992831 17.098374719125548 11 27.580466089877703 28.16018883670524 25.28341942044692 18.975925652970137 12 28.020427618428545 23.30035831043745 29.095995173041988 19.583218898092014 13 26.706163172812943 24.23022638560311 26.65091613584621 22.412694305737737 14 22.79952960488295 25.823377025503767 29.192916078584403 22.184177291028877 15 20.64044146730192 29.11031062407944 27.82531332280706 22.423934585811587 16 22.271978724058545 26.95991779749893 28.107274688055718 22.6608287903868 17 23.08318761618049 26.457710567029714 27.31112352961761 23.147978287172187 18 22.6258354656286 26.90763989111774 27.000319181537947 23.46620546171571 19 23.10768294351123 30.479928147039832 26.15963106431667 20.25275784513227 20 24.74993027845143 28.213209025732816 25.279071764946657 21.757788930869097 21 25.09127425541098 27.628714461892802 25.66495270068937 21.615058582006856 22 23.35984696252639 27.176240168731447 25.673541971311835 23.790370897430325 23 23.679134540850466 27.436675337234913 24.68620001124028 24.197990110674343 24 21.57603572288256 28.30472187199442 25.94288453157193 24.17635787355109 25 23.23153810507965 27.737511889777387 24.342417105585465 24.688532899557494 26 23.63480966282341 28.02509339506297 24.23202907203005 24.108067870083573 27 21.00924990217775 26.579550961415087 25.70821717493587 26.702981961471288 28 22.237621641568676 28.87924864029725 25.949883196523572 22.9332465216105 29 22.563589763710226 28.280756746553955 24.468923276605263 24.686730213130556 30 23.17247361450293 27.62765405811225 24.681216113471688 24.518656213913133 31 23.70829564481563 29.157180471179817 23.031864073201795 24.102659810802756 32 23.92027036054789 27.008378250270137 24.67114227755645 24.400209111625525 33 22.10878258223166 29.980265885643938 24.09672154963167 23.814229982492733 34 21.658535137009473 28.30694871993358 25.57842375219636 24.456092390860594 35 21.163008450357726 29.36417128914348 25.49475789391084 23.97806236658795 36 22.264449857216633 30.52860068056715 24.27921704026459 22.92773242195163 37 22.340056646769956 31.16706979683724 23.18700114629649 23.305872410096317 38 23.23249246848215 29.778365005826917 24.987884886807198 22.001257638883732 39 22.599961613383144 28.01777660897717 25.5175565751927 23.864705202446988 40 23.125921888536716 28.710538398811497 25.382673214306546 22.78086649834524 41 22.066472471387655 26.893324440080296 27.099997136909792 23.94020595162226 42 22.287460619254603 28.88105132672419 24.24337539248195 24.58811266153926 43 20.755389237113707 26.978050702146362 26.553677109169634 25.712882951570297 44 22.571118630552142 25.123828651473907 25.56230561473198 26.74274710324197 45 23.70225134326649 26.187201562611012 26.637555048211258 23.47299204591124 46 20.672041499962358 26.261960029139896 28.690072605846854 24.375925865050892 47 22.09330068703561 27.857549597735826 25.92347914238784 24.125670572840725 48 22.217261888982087 25.016197667747925 28.5974993558047 24.169041087465285 49 22.285763973205714 25.81234882618604 27.157258941059574 24.74462825954867 50 21.904972975609653 25.00516946843019 26.65547587210258 26.434381683857577 51 21.538815550185202 24.828294117834186 28.50036636950618 25.13252396247443 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1968.0 1 1642.5 2 1317.0 3 945.0 4 573.0 5 602.0 6 631.0 7 672.5 8 714.0 9 845.0 10 976.0 11 1120.5 12 1265.0 13 1239.0 14 1213.0 15 1389.0 16 1565.0 17 1790.5 18 2016.0 19 2357.0 20 2698.0 21 2578.0 22 2458.0 23 3067.5 24 3677.0 25 4350.0 26 6576.0 27 8129.0 28 9749.5 29 11370.0 30 13152.5 31 14935.0 32 17561.5 33 20188.0 34 21666.0 35 23144.0 36 26845.5 37 30547.0 38 31921.5 39 33296.0 40 37185.0 41 41074.0 42 51615.5 43 62157.0 44 67719.5 45 73282.0 46 81267.5 47 89253.0 48 84774.5 49 80296.0 50 75331.0 51 70366.0 52 65856.0 53 61346.0 54 55853.0 55 50360.0 56 47229.5 57 44099.0 58 43467.5 59 42836.0 60 41256.0 61 39676.0 62 36404.0 63 33132.0 64 29624.0 65 26116.0 66 21939.5 67 17763.0 68 16066.0 69 14369.0 70 11789.0 71 9209.0 72 8177.0 73 7145.0 74 5943.0 75 3712.5 76 2684.0 77 2538.5 78 2393.0 79 1868.5 80 1344.0 81 1136.5 82 929.0 83 658.5 84 388.0 85 276.5 86 165.0 87 118.0 88 71.0 89 53.5 90 36.0 91 44.5 92 53.0 93 42.0 94 31.0 95 19.5 96 8.0 97 8.0 98 8.0 99 6.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 943037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.836846112047947 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1986709681171 20.180951606018862 2 9.93991700515862 5.3351204606794065 3 3.53919867959145 2.8494279097247706 4 1.7884383674003401 1.9198418098722076 5 1.0454462965544347 1.4028240689520903 6 0.7204758254267198 1.160117931265656 7 0.5650512485142367 1.061493538125799 8 0.45528215095570307 0.9774669618208309 9 0.3683369834621647 0.8896502648295057 >10 4.901493003870971 31.07200175471204 >50 1.2950671054052998 23.868551224292744 >100 0.17506283407165263 6.765954101287136 >500 0.00477444092922689 0.8085062092438563 >1k 0.002387220464613445 1.1596917510033709 >5k 3.9787007743557416E-4 0.5484004081717441 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT 5136 0.5446233816912804 Illumina Single End Adapter 2 (95% over 22bp) AATGATACGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC 2377 0.25205797863710544 No Hit AATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT 2377 0.25205797863710544 No Hit AATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGT 2192 0.2324405086969016 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1468 0.1556672749849688 No Hit TGAATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC 1326 0.14060954130113665 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 1121 0.1188712637998297 No Hit CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC 975 0.10338936860377695 Illumina Single End Adapter 2 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0604037805515585E-4 0.0 0.0 0.6154583542321245 0.0 2 1.0604037805515585E-4 0.0 0.0 0.8671982117350645 0.0 3 1.0604037805515585E-4 0.0 0.0 1.2032401697918533 0.0 4 1.0604037805515585E-4 0.0 0.0 2.5558912322634213 0.0 5 1.0604037805515585E-4 0.0 0.0 2.7839840854600615 0.0 6 1.0604037805515585E-4 0.0 0.0 4.008432330862946 0.0 7 1.0604037805515585E-4 0.0 0.0 4.867783554621929 0.0 8 1.0604037805515585E-4 0.0 0.0 5.6004165266050006 0.0 9 1.0604037805515585E-4 0.0 0.0 6.734730450661003 0.0 10 1.0604037805515585E-4 0.0 0.0 8.080488888558985 0.0 11 1.0604037805515585E-4 0.0 0.0 9.59739649663799 0.0 12 1.0604037805515585E-4 1.0604037805515585E-4 0.0 10.217520627504541 0.0 13 1.0604037805515585E-4 1.0604037805515585E-4 0.0 10.579012276294568 0.0 14 1.0604037805515585E-4 1.0604037805515585E-4 0.0 10.896921329703925 0.0 15 1.0604037805515585E-4 1.0604037805515585E-4 0.0 11.174641079830378 0.0 16 1.0604037805515585E-4 1.0604037805515585E-4 0.0 11.6995409512034 0.0 17 1.0604037805515585E-4 1.0604037805515585E-4 0.0 12.296442239275871 0.0 18 1.0604037805515585E-4 1.0604037805515585E-4 0.0 13.09831957812896 0.0 19 1.0604037805515585E-4 1.0604037805515585E-4 0.0 13.45556961179678 0.0 20 1.0604037805515585E-4 1.0604037805515585E-4 0.0 13.92882781905694 0.0 21 1.0604037805515585E-4 1.0604037805515585E-4 0.0 14.386710171499104 0.0 22 1.0604037805515585E-4 1.0604037805515585E-4 0.0 14.859968378759264 0.0 23 1.0604037805515585E-4 1.0604037805515585E-4 0.0 15.375006494973157 0.0 24 1.0604037805515585E-4 1.0604037805515585E-4 0.0 15.725045782933226 0.0 25 1.0604037805515585E-4 1.0604037805515585E-4 0.0 16.04062194802537 0.0 26 1.0604037805515585E-4 1.0604037805515585E-4 0.0 16.34665447909255 0.0 27 1.0604037805515585E-4 1.0604037805515585E-4 0.0 16.669229309136334 0.0 28 1.0604037805515585E-4 1.0604037805515585E-4 0.0 16.99392494674122 0.0 29 1.0604037805515585E-4 1.0604037805515585E-4 0.0 17.33537496407882 0.0 30 1.0604037805515585E-4 1.0604037805515585E-4 0.0 17.81457143251007 0.0 31 1.0604037805515585E-4 1.0604037805515585E-4 0.0 18.134919414614696 0.0 32 1.0604037805515585E-4 1.0604037805515585E-4 0.0 18.46428082885401 0.0 33 1.0604037805515585E-4 1.0604037805515585E-4 0.0 18.787703981922235 0.0 34 1.0604037805515585E-4 1.0604037805515585E-4 0.0 19.09649356281885 0.0 35 1.0604037805515585E-4 1.0604037805515585E-4 0.0 19.44451808359587 0.0 36 1.0604037805515585E-4 1.0604037805515585E-4 0.0 19.773243255566854 0.0 37 1.0604037805515585E-4 1.0604037805515585E-4 0.0 20.111936223075023 0.0 38 1.0604037805515585E-4 1.0604037805515585E-4 0.0 20.456249330620114 0.0 39 1.0604037805515585E-4 1.0604037805515585E-4 0.0 20.80501613404352 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTAG 45 3.8380676E-10 45.000004 1 TAACGAA 20 7.032642E-4 45.0 45 TATGCGA 20 7.032642E-4 45.0 16 ATCCGTG 20 7.032642E-4 45.0 11 TGCGCGT 25 3.8902712E-5 45.0 37 CGTAGCA 25 3.8902712E-5 45.0 29 TATAGCG 20 7.032642E-4 45.0 1 CACGTAA 20 7.032642E-4 45.0 34 TCGACGT 20 7.032642E-4 45.0 12 TATCGCG 20 7.032642E-4 45.0 1 TTACGTA 30 2.1650867E-6 44.999996 45 ATTCACG 80 0.0 42.1875 1 TACGAAT 120 0.0 41.249996 11 ATTACCG 55 6.002665E-11 40.909092 1 ACTACCG 55 6.002665E-11 40.909092 1 TCGGGTC 50 1.0804797E-9 40.5 5 CATAACG 50 1.0804797E-9 40.5 1 CGAGATA 350 0.0 40.5 19 CCCGATC 95 0.0 40.263157 41 TCGATAG 45 1.927583E-8 40.000004 1 >>END_MODULE