FastQCFastQC Report
Sat 18 Jun 2016
SRR3553088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003106
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT39880.397565162604949Illumina Single End Adapter 2 (95% over 22bp)
AACCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATC21890.2182222018410816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16930.16877578241980407No Hit
AACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT14950.14903709079598765No Hit
AACCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGA14910.1486383293490419No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG11740.1170364846785883No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA11540.11504267744385938No Hit
AACCCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT11160.1112544436978744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG253.8904498E-545.01
TAGACGC207.032858E-445.016
TAGCGTA253.8904498E-545.029
CATTCGC207.032858E-445.012
ACGATAG406.8139343E-945.01
CGTACCG207.032858E-445.01
CGTCGAA253.8904498E-545.027
ACCGTCC207.032858E-445.026
CGACTTA253.8904498E-545.030
TAAATCG207.032858E-445.01
ACGACAT207.032858E-445.031
AAACTCG302.165225E-644.9999961
GTCGCGA302.165225E-644.99999617
CAACGAA302.165225E-644.99999643
GTATGCG302.165225E-644.9999961
CTAGGTC302.165225E-644.99999629
AACCCCG17850.043.1092451
CCAAACG850.042.352941
AGGGATC9700.042.2164966
ACCCCGG19250.042.19482