##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003106
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.881610717112647	33.0	31.0	34.0	30.0	34.0
2	32.09131637135059	34.0	31.0	34.0	30.0	34.0
3	32.24988984215028	34.0	31.0	34.0	30.0	34.0
4	35.89692016596452	37.0	35.0	37.0	35.0	37.0
5	35.681114458491926	37.0	35.0	37.0	33.0	37.0
6	35.00466650583288	37.0	35.0	37.0	33.0	37.0
7	35.44482138477888	37.0	35.0	37.0	33.0	37.0
8	35.844126144196125	37.0	35.0	37.0	35.0	37.0
9	37.468671306920704	39.0	37.0	39.0	35.0	39.0
10	37.06011328812708	39.0	37.0	39.0	33.0	39.0
11	37.084493562993345	39.0	37.0	39.0	33.0	39.0
12	37.070304633807396	39.0	37.0	39.0	33.0	39.0
13	37.059895963138494	39.0	37.0	39.0	33.0	39.0
14	38.25802158495712	40.0	38.0	41.0	33.0	41.0
15	38.284581091130946	40.0	38.0	41.0	33.0	41.0
16	38.16776292834456	40.0	37.0	41.0	33.0	41.0
17	38.167027213474945	40.0	37.0	41.0	33.0	41.0
18	38.14212356420956	40.0	37.0	41.0	33.0	41.0
19	38.18390778242778	40.0	37.0	41.0	34.0	41.0
20	38.07924785615877	40.0	37.0	41.0	33.0	41.0
21	38.056345989357055	40.0	37.0	41.0	33.0	41.0
22	38.030784383704216	40.0	37.0	41.0	33.0	41.0
23	37.92920588651648	40.0	37.0	41.0	33.0	41.0
24	37.83109860772441	40.0	37.0	41.0	33.0	41.0
25	37.808389143320845	40.0	37.0	41.0	33.0	41.0
26	37.797137092191655	40.0	37.0	41.0	33.0	41.0
27	37.715686079038505	40.0	37.0	41.0	33.0	41.0
28	37.57810241390242	40.0	37.0	41.0	33.0	41.0
29	37.38156884716072	40.0	36.0	41.0	32.0	41.0
30	37.496050267868	40.0	36.0	41.0	32.0	41.0
31	37.478075098743304	40.0	36.0	41.0	32.0	41.0
32	37.46878096631861	40.0	36.0	41.0	32.0	41.0
33	37.23424543368298	40.0	36.0	41.0	32.0	41.0
34	37.235299160806534	40.0	36.0	41.0	31.0	41.0
35	37.1698205374108	39.0	36.0	41.0	31.0	41.0
36	37.16171870171248	39.0	36.0	41.0	31.0	41.0
37	37.08580249744294	39.0	36.0	41.0	31.0	41.0
38	36.92331418613786	39.0	35.0	41.0	31.0	41.0
39	36.91136031486204	39.0	35.0	41.0	31.0	41.0
40	36.83423386960102	39.0	35.0	41.0	31.0	41.0
41	36.79234098888851	39.0	35.0	41.0	31.0	41.0
42	36.71979232503843	39.0	35.0	41.0	31.0	41.0
43	36.6665536842567	39.0	35.0	41.0	31.0	41.0
44	36.61230418320696	39.0	35.0	40.0	31.0	41.0
45	36.428840022888906	39.0	35.0	40.0	30.0	41.0
46	36.31624574072929	39.0	35.0	40.0	30.0	41.0
47	36.17022827099031	38.0	35.0	40.0	30.0	41.0
48	36.07299128905619	38.0	35.0	40.0	30.0	41.0
49	35.97125229038606	38.0	35.0	40.0	29.0	41.0
50	35.88083113848387	38.0	35.0	40.0	29.0	41.0
51	34.69800798719178	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	5.0
12	7.0
13	16.0
14	29.0
15	32.0
16	61.0
17	142.0
18	246.0
19	507.0
20	1008.0
21	1691.0
22	2549.0
23	3172.0
24	3931.0
25	4859.0
26	5914.0
27	7332.0
28	9241.0
29	12073.0
30	15644.0
31	20790.0
32	27772.0
33	38018.0
34	57770.0
35	71993.0
36	91407.0
37	135893.0
38	213807.0
39	277127.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.010048788463035	32.847575430712205	20.924907238118404	26.217468542706353
2	23.668286302743677	31.162708627004527	19.93049587979735	25.23850919045445
3	21.06078520116518	29.647215747887063	20.91124965856051	28.380749392387244
4	20.483278935625947	31.040986695324325	19.155503007658215	29.320231361391517
5	18.491365817770006	32.46915081756065	17.513702440220673	31.52578092444866
6	19.076647931524686	38.67836499831523	18.482194304490253	23.76279276566983
7	86.29696163715501	5.1036480690973836	4.669695924458631	3.9296943692889883
8	87.71625331719679	4.175630491692802	3.802489467713283	4.305626723397128
9	80.55659122764692	8.053784943964047	6.992880114364783	4.3967437140242405
10	32.50304554055105	39.02169860413556	12.720290776847113	15.754965078466284
11	24.33202473118494	30.233594455620842	26.496501865206668	18.937878947987553
12	25.8997553598523	22.570795110387138	31.231893738049614	20.297555791710945
13	27.82517500643003	22.63918269853834	27.82068894015189	21.714953354879743
14	21.666603529437566	28.31046768736305	27.61143887086709	22.411489912332296
15	20.540002751453983	29.40437002669708	28.96782593265318	21.087801289195756
16	22.17452592248476	28.51363664458193	27.069821135552974	22.24201629738034
17	21.96108885800703	26.393023269724235	27.91798673320666	23.727901139062073
18	23.688922207623122	25.94062840816424	27.202209935938974	23.16823944827366
19	23.28916385705997	29.863942594302095	26.403989209515245	20.442904339122688
20	24.757204123990885	27.353440214693165	25.579151156507884	22.310204504808066
21	25.508769761121957	27.588709468391176	26.149878477449047	20.752642293037823
22	22.55364836816847	26.69648073084998	25.70426256048713	25.045608340494425
23	22.372710361616818	27.476458121075936	25.883107069442314	24.267724447864932
24	21.778854876752806	27.69158992170319	25.953289084104767	24.576266117439232
25	22.623331931022246	28.12454516272458	25.08099841891086	24.171124487342315
26	23.857697990042926	28.56228554110932	24.310890374496815	23.269126094350945
27	21.837173738368627	27.1678167611399	25.239406403710074	25.7556030967814
28	22.42823789310402	26.89596114468461	28.557001951937284	22.11879901027409
29	23.74544664272769	28.140495620602408	24.576565188524444	23.53749254814546
30	22.469310322139435	25.541966651580196	27.200714580512926	24.788008445767446
31	24.112506554641282	26.720805179113672	24.710648725059965	24.456039541185078
32	23.644061544841723	27.018081837811756	25.291345082174765	24.046511535171756
33	22.98879679714806	27.486128086164374	25.633083642207303	23.891991474480264
34	22.030872111222543	26.003832097505146	26.931450913462783	25.033844877809525
35	22.754225375982198	26.602672100455983	26.29901525860677	24.34408726495505
36	23.425639962277167	27.03253694026354	25.83884454883133	23.70297854862796
37	22.961381947670535	27.871830095722682	24.120681164303672	25.046106792303107
38	22.599206863482024	27.621208526317258	25.860277976604667	23.91930663359605
39	22.727009907228148	25.825087278911703	25.478962342962756	25.968940470897394
40	23.882620580477038	26.922678161629975	26.349358891283675	22.845342366609312
41	21.685245627082285	25.82917458374289	28.454121498625266	24.031458290549555
42	23.433316120130872	26.93015493876021	25.811429699353805	23.82509924175511
43	22.435914050957724	26.180981870310816	26.7003686549577	24.682735423773757
44	22.84205258467201	24.4577342773346	26.562795955761402	26.137417182231985
45	23.659513550910873	25.665383319409912	26.562397194314457	24.112705935364755
46	22.242714129912493	25.06793898152339	27.882995416237165	24.806351472326952
47	22.236633017846568	27.629183755256175	25.97322715645206	24.1609560704452
48	22.620640291255363	25.12565970096879	28.32003796208975	23.9336620456861
49	22.373906645957657	25.776139311299108	27.380356612362007	24.469597430381235
50	21.396143578046587	24.686224586434534	27.89027281264393	26.02735902287495
51	21.671687737886124	24.504090295542046	28.15156125075515	25.672660715816676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2103.0
1	1732.5
2	1362.0
3	987.5
4	613.0
5	599.5
6	586.0
7	619.0
8	652.0
9	738.0
10	824.0
11	927.5
12	1031.0
13	1131.5
14	1232.0
15	1479.0
16	1726.0
17	1686.5
18	1647.0
19	1665.5
20	1684.0
21	1851.5
22	2019.0
23	3416.0
24	4813.0
25	6333.5
26	8729.0
27	9604.0
28	12308.5
29	15013.0
30	16884.0
31	18755.0
32	21696.5
33	24638.0
34	26821.5
35	29005.0
36	33238.5
37	37472.0
38	38669.0
39	39866.0
40	41872.5
41	43879.0
42	45229.0
43	46579.0
44	48513.5
45	50448.0
46	58900.5
47	67353.0
48	73272.0
49	79191.0
50	84713.0
51	90235.0
52	85267.0
53	80299.0
54	74006.0
55	67713.0
56	62916.5
57	58120.0
58	53602.0
59	49084.0
60	46406.5
61	43729.0
62	39950.0
63	36171.0
64	30426.0
65	24681.0
66	21344.5
67	18008.0
68	16260.5
69	14513.0
70	12230.0
71	9947.0
72	8917.5
73	7888.0
74	6501.0
75	4352.5
76	3591.0
77	2770.5
78	1950.0
79	1513.0
80	1076.0
81	814.5
82	553.0
83	451.5
84	350.0
85	213.0
86	76.0
87	53.0
88	30.0
89	24.5
90	19.0
91	12.0
92	5.0
93	3.0
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1003106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.1898234133648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95108718225008	17.38102476396089
2	10.39856472310549	4.822817593625219
3	3.7624568451176184	2.617521295160496
4	1.8013510730823616	1.6709205316102063
5	1.0720348262182817	1.243014915648959
6	0.6921467452794089	0.9630456479498808
7	0.4796702246226991	0.7786427463954595
8	0.3661168217354015	0.6792147555765057
9	0.2983161425840031	0.6226108801091268
>10	3.9409078317511477	23.051104621065686
>50	1.7304142625656695	28.75101116483466
>100	0.49903989626364914	15.154066091066603
>500	0.004385236346780749	0.8107971449360079
>1k	0.003508189077424599	1.4542078480603178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	3988	0.397565162604949	Illumina Single End Adapter 2 (95% over 22bp)
AACCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATC	2189	0.2182222018410816	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1693	0.16877578241980407	No Hit
AACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	1495	0.14903709079598765	No Hit
AACCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGA	1491	0.1486383293490419	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1174	0.1170364846785883	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1154	0.11504267744385938	No Hit
AACCCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	1116	0.1112544436978744	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44103016032203973	0.0
2	0.0	0.0	0.0	0.6340307006438004	0.0
3	0.0	0.0	0.0	0.848464668738897	0.0
4	0.0	0.0	0.0	1.8546394897448526	0.0
5	0.0	0.0	0.0	2.008262337180717	0.0
6	0.0	0.0	0.0	2.9819381002605905	0.0
7	0.0	0.0	0.0	3.62105300935295	0.0
8	0.0	0.0	0.0	4.214709113493489	0.0
9	0.0	0.0	0.0	4.965875989177614	0.0
10	9.96903617364466E-5	0.0	0.0	6.098557879227121	0.0
11	9.96903617364466E-5	0.0	0.0	7.0742274495417234	0.0
12	9.96903617364466E-5	0.0	0.0	7.657515756061672	0.0
13	9.96903617364466E-5	0.0	0.0	7.901059309783811	0.0
14	9.96903617364466E-5	0.0	0.0	8.148092026166726	0.0
15	9.96903617364466E-5	0.0	0.0	8.350862221938659	0.0
16	9.96903617364466E-5	0.0	0.0	8.769860812316944	0.0
17	9.96903617364466E-5	0.0	0.0	9.282767723450961	0.0
18	9.96903617364466E-5	0.0	0.0	10.225041022583854	0.0
19	9.96903617364466E-5	0.0	0.0	10.49789354265651	0.0
20	9.96903617364466E-5	0.0	0.0	11.022962677922374	0.0
21	9.96903617364466E-5	0.0	0.0	11.39809750913662	0.0
22	9.96903617364466E-5	0.0	0.0	11.824971638092086	0.0
23	9.96903617364466E-5	0.0	0.0	12.265104585158499	0.0
24	9.96903617364466E-5	0.0	0.0	12.575141610158846	0.0
25	9.96903617364466E-5	0.0	0.0	12.839420759122167	0.0
26	9.96903617364466E-5	0.0	0.0	13.083662145376461	0.0
27	9.96903617364466E-5	0.0	0.0	13.466971586253099	0.0
28	9.96903617364466E-5	0.0	0.0	13.77162533171968	0.0
29	9.96903617364466E-5	0.0	0.0	14.054646268689451	0.0
30	9.96903617364466E-5	0.0	0.0	14.570145129228615	0.0
31	9.96903617364466E-5	0.0	0.0	14.855957396327009	0.0
32	9.96903617364466E-5	0.0	0.0	15.143065638127975	0.0
33	9.96903617364466E-5	0.0	0.0	15.429176976311576	0.0
34	9.96903617364466E-5	0.0	0.0	15.697044978297408	0.0
35	9.96903617364466E-5	0.0	0.0	16.00249624665788	0.0
36	9.96903617364466E-5	0.0	0.0	16.30047073788812	0.0
37	9.96903617364466E-5	0.0	0.0	16.58807743149777	0.0
38	9.96903617364466E-5	0.0	0.0	16.87638195763957	0.0
39	9.96903617364466E-5	0.0	0.0	17.174256758508076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.8904498E-5	45.0	1
TAGACGC	20	7.032858E-4	45.0	16
TAGCGTA	25	3.8904498E-5	45.0	29
CATTCGC	20	7.032858E-4	45.0	12
ACGATAG	40	6.8139343E-9	45.0	1
CGTACCG	20	7.032858E-4	45.0	1
CGTCGAA	25	3.8904498E-5	45.0	27
ACCGTCC	20	7.032858E-4	45.0	26
CGACTTA	25	3.8904498E-5	45.0	30
TAAATCG	20	7.032858E-4	45.0	1
ACGACAT	20	7.032858E-4	45.0	31
AAACTCG	30	2.165225E-6	44.999996	1
GTCGCGA	30	2.165225E-6	44.999996	17
CAACGAA	30	2.165225E-6	44.999996	43
GTATGCG	30	2.165225E-6	44.999996	1
CTAGGTC	30	2.165225E-6	44.999996	29
AACCCCG	1785	0.0	43.109245	1
CCAAACG	85	0.0	42.35294	1
AGGGATC	970	0.0	42.216496	6
ACCCCGG	1925	0.0	42.1948	2
>>END_MODULE