SRR3553086_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522413
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	5304	1.0152886700752086	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGT	5019	0.9607341318075928	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	4157	0.7957305809771196	TruSeq Adapter, Index 10 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	4056	0.7763972182928067	RNA PCR Primer, Index 35 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2064	0.39508970871704957	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT	1128	0.215921119880248	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTTGTCTC	1057	0.20233034017147355	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	903	0.17285174756370916	RNA PCR Primer, Index 35 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	898	0.17189465040111943	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTAT	848	0.1623236787752219	No Hit
AACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	846	0.16194083991018599	RNA PCR Primer, Index 10 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	668	0.12786818092199084	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT	663	0.12691108375940108	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	599	0.11466024007825226	RNA PCR Primer, Index 10 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	545	0.10432359072228294	No Hit
TTCGCGGGGAAGTTCAACTTAGTAGATGATTTTACAGTTCCATGTGTACAG	534	0.10221797696458548	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	20	7.0297485E-4	45.000004	1
TTAGGAT	20	7.0297485E-4	45.000004	4
AGGTAAC	35	1.2102282E-7	45.000004	44
AAATCGG	20	7.0297485E-4	45.000004	4
ATTAGGC	20	7.0297485E-4	45.000004	29
ATAGGCG	20	7.0297485E-4	45.000004	1
GCGCGAA	20	7.0297485E-4	45.000004	42
GACGTAC	20	7.0297485E-4	45.000004	45
CGTTGAT	40	6.8030204E-9	45.000004	25
GATCCGT	20	7.0297485E-4	45.000004	43
ATTGTTG	20	7.0297485E-4	45.000004	1
ATTGTCG	20	7.0297485E-4	45.000004	33
CGCGATG	20	7.0297485E-4	45.000004	1
CGATTGT	35	1.2102282E-7	45.000004	27
TTCCGCA	20	7.0297485E-4	45.000004	17
CGCTGTT	20	7.0297485E-4	45.000004	27
ATCCGTC	20	7.0297485E-4	45.000004	44
AACGGTT	20	7.0297485E-4	45.000004	25
AAACGGT	20	7.0297485E-4	45.000004	24
AATGCGC	20	7.0297485E-4	45.000004	10