##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522413
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.699639940047433	31.0	31.0	34.0	30.0	34.0
2	31.896323406959628	33.0	31.0	34.0	30.0	34.0
3	32.05792543447426	33.0	31.0	34.0	30.0	34.0
4	35.734202632782875	37.0	35.0	37.0	33.0	37.0
5	35.486404434805415	37.0	35.0	37.0	33.0	37.0
6	34.89514043486667	37.0	35.0	37.0	32.0	37.0
7	35.22362383784477	37.0	35.0	37.0	33.0	37.0
8	35.63544551915821	37.0	35.0	37.0	33.0	37.0
9	37.33572097172161	39.0	37.0	39.0	34.0	39.0
10	36.897984927633885	39.0	37.0	39.0	33.0	39.0
11	36.913551155886246	39.0	37.0	39.0	33.0	39.0
12	36.758068807629215	39.0	35.0	39.0	32.0	39.0
13	36.74734166263091	39.0	35.0	39.0	33.0	39.0
14	37.87191168673061	40.0	37.0	41.0	33.0	41.0
15	37.907247713973426	40.0	37.0	41.0	33.0	41.0
16	37.74037782367591	40.0	36.0	41.0	33.0	41.0
17	37.7569049774795	40.0	37.0	41.0	33.0	41.0
18	37.69605465407637	39.0	36.0	41.0	33.0	41.0
19	37.676707892031786	40.0	36.0	41.0	32.0	41.0
20	37.66145559164875	39.0	36.0	41.0	32.0	41.0
21	37.67192432041316	39.0	36.0	41.0	33.0	41.0
22	37.642449556194045	39.0	36.0	41.0	33.0	41.0
23	37.51754263389311	39.0	36.0	41.0	32.0	41.0
24	37.36492200615222	39.0	36.0	41.0	32.0	41.0
25	37.43913532013943	39.0	36.0	41.0	32.0	41.0
26	37.363737119864936	39.0	36.0	41.0	32.0	41.0
27	37.23697151487425	39.0	36.0	41.0	32.0	41.0
28	37.20035489162789	39.0	36.0	41.0	32.0	41.0
29	37.005151096929055	39.0	35.0	41.0	31.0	41.0
30	36.92897573375854	39.0	35.0	41.0	31.0	41.0
31	36.94033456288415	39.0	35.0	41.0	31.0	41.0
32	36.84158702023112	39.0	35.0	41.0	31.0	41.0
33	36.5867752142462	39.0	35.0	40.0	30.0	41.0
34	36.607337489687275	39.0	35.0	40.0	30.0	41.0
35	36.47297444741995	39.0	35.0	40.0	30.0	41.0
36	36.43497003328784	39.0	35.0	40.0	30.0	41.0
37	36.21671359633087	39.0	35.0	40.0	30.0	41.0
38	36.135304825875316	38.0	35.0	40.0	30.0	41.0
39	36.17486356579947	39.0	35.0	40.0	30.0	41.0
40	36.01798002729641	38.0	35.0	40.0	29.0	41.0
41	36.02628954486202	38.0	35.0	40.0	29.0	41.0
42	35.92946385331146	38.0	35.0	40.0	29.0	41.0
43	35.85005350173139	38.0	35.0	40.0	29.0	41.0
44	35.64928514412926	38.0	35.0	40.0	28.0	41.0
45	35.407170189103255	38.0	34.0	40.0	27.0	41.0
46	35.218591420963875	38.0	34.0	40.0	26.0	41.0
47	35.08020091383637	38.0	34.0	40.0	26.0	41.0
48	35.00056468732593	37.0	34.0	40.0	26.0	41.0
49	34.90558810749349	37.0	34.0	40.0	26.0	41.0
50	34.837733747054536	37.0	34.0	40.0	26.0	41.0
51	33.41712017120554	36.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	3.0
11	2.0
12	7.0
13	7.0
14	14.0
15	36.0
16	55.0
17	129.0
18	240.0
19	458.0
20	840.0
21	1353.0
22	1951.0
23	2431.0
24	2950.0
25	3525.0
26	4180.0
27	5161.0
28	6646.0
29	8366.0
30	10640.0
31	13887.0
32	17923.0
33	23847.0
34	35108.0
35	42782.0
36	49950.0
37	70810.0
38	102508.0
39	116571.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.662579223717632	34.808283867361645	19.360927082595573	25.16820982632515
2	22.936642082030883	33.8906191078706	19.672557918734796	23.50018089136373
3	20.314195856534965	29.786395055253216	23.700214198344987	26.199194889866828
4	24.074822027782616	30.83001380134108	17.980984393573667	27.11417977730263
5	18.042621450844447	35.41470062957852	17.883551902422028	28.65912601715501
6	19.797363388736496	37.331766246245785	22.745796907810487	20.12507345720723
7	79.0541200161558	9.262977758976136	6.581000089967133	5.10190213490093
8	80.81843292567375	5.3388793923581535	5.135974793889126	8.706712888078973
9	75.54903878731962	8.74997367982803	9.738463629350724	5.962523903501635
10	35.3676114491791	32.379554107573895	14.07507087304489	18.177763570202117
11	27.265975387289366	28.67692802437918	24.060082731478737	19.997013856852718
12	26.97903765794496	24.099132295712398	31.07139370574622	17.850436340596424
13	26.389082966924637	25.467015560485667	25.514104740885085	22.62979673170461
14	20.649371282873894	27.416813900113514	30.290019582207943	21.643795234804646
15	19.8251192064516	29.249080708175335	27.97078173782046	22.95501834755261
16	21.588857857671996	27.477685279654217	28.111283601288633	22.82217326138515
17	20.009456119966387	28.767852254825204	27.43710435996041	23.785587265247994
18	21.786019873165486	28.201442154004592	25.26104825109636	24.75148972173357
19	22.207334044137493	30.875763045712873	27.32052992555698	19.59637298459265
20	24.543991056884114	29.196631783665417	25.291101102001672	20.9682760574488
21	22.916925880481536	28.945298068769343	25.890626764647894	22.247149286101227
22	22.137657370700957	28.63500716865775	25.679108291715558	23.548227168925735
23	22.967460610666272	28.11568624823655	23.803006433607127	25.11384670749005
24	20.095403445166944	30.81929431312008	25.280381613780666	23.804920627932308
25	23.78214171546267	26.73172375113177	24.321561676298252	25.16457285710731
26	24.364631048614793	28.494313885757055	23.98485489449918	23.156200171128972
27	20.722493506095752	26.89404742990699	26.77517596231334	25.608283101683917
28	24.606585211317483	27.04603445932624	27.139638561827518	21.207741767528756
29	24.2292975098246	28.992770088033797	24.63931793427805	22.138614467863547
30	25.290718263136636	25.946329819510616	25.775392266272085	22.98755965108066
31	26.007009779618805	27.380635627367617	24.384155830731626	22.228198762281952
32	24.928552696812677	24.725073840046093	25.92757071512386	24.418802748017374
33	26.791255194644847	24.90385959001786	25.844877520276103	22.460007695061186
34	23.23678775221903	24.47393154458254	29.458876406215005	22.83040429698342
35	26.493789396511957	23.430312798494676	26.709136258094652	23.36676154689872
36	25.301437751357643	25.840666292760712	29.98317423188167	18.874721723999976
37	23.076378267768987	26.386594514301905	27.99202929482995	22.544997923099157
38	23.212477484289252	25.667240286899446	29.110684458464853	22.00959777034645
39	23.54861000779077	23.11045092675718	30.092283308416906	23.24865575703514
40	25.40193295342957	24.403872032280972	26.084534649788576	24.109660364500883
41	21.640923943316878	22.151056730977214	30.935677328090993	25.272341997614916
42	23.69638580969463	24.784031025261623	25.883927084509768	25.635656080533987
43	23.317375333309087	22.631519506597268	28.98051924435265	25.070585915740995
44	23.158688623751704	22.190871972940947	28.426742826078215	26.223696577229127
45	25.64446137442981	23.01244417730799	28.422148759697784	22.920945688564412
46	21.11700895651525	22.7278034811538	33.045119474438806	23.110068087892145
47	23.713613558621244	24.65348297228438	27.467157210865732	24.165746258228644
48	21.422514370813897	22.83155281357853	32.13415439508588	23.611778420521695
49	22.089419673706434	22.60204091398951	28.96061162337078	26.34792778893328
50	20.966936121421178	22.688945336352656	28.316868071812912	28.027250470413257
51	21.062262998815115	21.43897644201044	31.929144182859154	25.56961637631529
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2522.0
1	2052.0
2	1582.0
3	1085.0
4	588.0
5	567.0
6	546.0
7	564.0
8	582.0
9	653.5
10	725.0
11	802.5
12	880.0
13	914.5
14	949.0
15	999.0
16	1049.0
17	1125.0
18	1201.0
19	1246.0
20	1291.0
21	1646.0
22	2001.0
23	2216.0
24	2431.0
25	2634.5
26	3525.5
27	4213.0
28	5486.0
29	6759.0
30	7378.0
31	7997.0
32	8808.5
33	9620.0
34	10841.5
35	12063.0
36	14097.5
37	16132.0
38	16452.5
39	16773.0
40	18030.5
41	19288.0
42	20444.0
43	21600.0
44	31743.0
45	41886.0
46	45142.5
47	48399.0
48	49745.0
49	51091.0
50	48565.0
51	46039.0
52	42040.5
53	38042.0
54	33825.5
55	29609.0
56	28057.5
57	26506.0
58	25518.0
59	24530.0
60	22542.5
61	20555.0
62	19570.0
63	18585.0
64	16060.0
65	13535.0
66	11521.5
67	9508.0
68	8252.0
69	6996.0
70	5873.5
71	4751.0
72	3935.5
73	3120.0
74	2639.5
75	1930.5
76	1702.0
77	1387.0
78	1072.0
79	708.0
80	344.0
81	266.0
82	188.0
83	124.5
84	61.0
85	67.5
86	74.0
87	44.0
88	14.0
89	9.5
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	3.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	522413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.474588307144842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97559317615945	20.873981439061037
2	10.304775495543481	5.662389286752233
3	3.8036405891843614	3.135103777685585
4	1.8476883929921577	2.030579116693983
5	1.0683603921153102	1.467638096851399
6	0.654217917038495	1.0784620760274293
7	0.49509420131682674	0.9521756548104153
8	0.3587191548526322	0.7884528877970407
9	0.2998207163738709	0.741370567349281
>10	3.696692510718708	25.070328360027027
>50	1.272150914407537	24.548057269975903
>100	0.21190703253919416	7.948375487906849
>500	0.006378472551346982	1.2664437573088227
>1k	0.0035435958618594346	2.4265716641424584
>5k	0.0014174383447437737	2.0100705576105438
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	5304	1.0152886700752086	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGT	5019	0.9607341318075928	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	4157	0.7957305809771196	TruSeq Adapter, Index 10 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	4056	0.7763972182928067	RNA PCR Primer, Index 35 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2064	0.39508970871704957	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT	1128	0.215921119880248	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTTGTCTC	1057	0.20233034017147355	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	903	0.17285174756370916	RNA PCR Primer, Index 35 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	898	0.17189465040111943	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTAT	848	0.1623236787752219	No Hit
AACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	846	0.16194083991018599	RNA PCR Primer, Index 10 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	668	0.12786818092199084	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT	663	0.12691108375940108	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	599	0.11466024007825226	RNA PCR Primer, Index 10 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	545	0.10432359072228294	No Hit
TTCGCGGGGAAGTTCAACTTAGTAGATGATTTTACAGTTCCATGTGTACAG	534	0.10221797696458548	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9316383780648644	0.0
2	0.0	0.0	0.0	1.2132163632987694	0.0
3	0.0	0.0	0.0	1.680662617507604	0.0
4	0.0	0.0	0.0	3.8657154397000073	0.0
5	0.0	0.0	0.0	4.167392465348297	0.0
6	0.0	0.0	0.0	5.649170292469751	0.0
7	0.0	0.0	0.0	6.744472285337463	0.0
8	0.0	0.0	0.0	7.5553250014835	0.0
9	0.0	0.0	0.0	9.615380934241683	0.0
10	0.0	0.0	0.0	11.33030763017	0.0
11	0.0	0.0	0.0	13.852067234161478	0.0
12	0.0	0.0	0.0	14.66158001427989	0.0
13	0.0	0.0	0.0	15.03427364939234	0.0
14	0.0	0.0	0.0	15.560102830519149	0.0
15	0.0	0.0	0.0	15.92226839684311	0.0
16	0.0	0.0	0.0	16.56582052896846	0.0
17	0.0	0.0	0.0	17.310824960328322	0.0
18	0.0	0.0	0.0	18.603097549257004	0.0
19	0.0	0.0	0.0	19.046807793833615	0.0
20	0.0	0.0	0.0	19.53282173299669	0.0
21	0.0	0.0	0.0	20.036063421086382	0.0
22	0.0	0.0	0.0	20.55385298604744	0.0
23	0.0	0.0	0.0	21.04024976407555	0.0
24	0.0	0.0	0.0	21.416963207270875	0.0
25	0.0	0.0	0.0	21.730699657167797	0.0
26	0.0	0.0	0.0	22.04041629898184	0.0
27	0.0	0.0	0.0	22.391479538219762	0.0
28	0.0	0.0	0.0	22.744074132917827	0.0
29	0.0	0.0	0.0	23.102411310591428	0.0
30	0.0	0.0	0.0	23.583639763941555	0.0
31	0.0	0.0	0.0	23.922643578930845	0.0
32	0.0	0.0	0.0	24.268538493490784	0.0
33	0.0	0.0	0.0	24.610030761102806	0.0
34	0.0	0.0	0.0	24.935826635248358	0.0
35	0.0	0.0	0.0	25.28401858299851	0.0
36	0.0	0.0	0.0	25.61000587657658	0.0
37	0.0	0.0	0.0	25.938864461642417	0.0
38	0.0	0.0	0.0	26.300264350236308	0.0
39	0.0	0.0	0.0	26.666449724643147	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	20	7.0297485E-4	45.000004	1
TTAGGAT	20	7.0297485E-4	45.000004	4
AGGTAAC	35	1.2102282E-7	45.000004	44
AAATCGG	20	7.0297485E-4	45.000004	4
ATTAGGC	20	7.0297485E-4	45.000004	29
ATAGGCG	20	7.0297485E-4	45.000004	1
GCGCGAA	20	7.0297485E-4	45.000004	42
GACGTAC	20	7.0297485E-4	45.000004	45
CGTTGAT	40	6.8030204E-9	45.000004	25
GATCCGT	20	7.0297485E-4	45.000004	43
ATTGTTG	20	7.0297485E-4	45.000004	1
ATTGTCG	20	7.0297485E-4	45.000004	33
CGCGATG	20	7.0297485E-4	45.000004	1
CGATTGT	35	1.2102282E-7	45.000004	27
TTCCGCA	20	7.0297485E-4	45.000004	17
CGCTGTT	20	7.0297485E-4	45.000004	27
ATCCGTC	20	7.0297485E-4	45.000004	44
AACGGTT	20	7.0297485E-4	45.000004	25
AAACGGT	20	7.0297485E-4	45.000004	24
AATGCGC	20	7.0297485E-4	45.000004	10
>>END_MODULE