SRR3553081_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553081_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465603
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	6039	1.2970277253368212	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	3660	0.7860774092950431	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGT	3545	0.7613782557242974	No Hit
AATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	3005	0.6453996215660123	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2410	0.5176083487434574	No Hit
AAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	1871	0.4018444898336136	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	1280	0.27491231800482385	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	951	0.20425126126764648	No Hit
AATGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	816	0.1752566027280752	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	752	0.16151098682783402	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	730	0.15678593136212612	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	691	0.14840969667291662	No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	690	0.14819492142447538	No Hit
AACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	674	0.14475851744941506	No Hit
AATGATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	643	0.13810048474773573	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	624	0.1340197550273516	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTAT	621	0.1333754292820278	No Hit
ACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCG	569	0.12220711636308185	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	498	0.10695807372375178	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCCTGTTC	494	0.10609897272998671	No Hit
AACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	484	0.10395122024557402	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGA	25	3.887218E-5	45.000004	27
TTTACGT	25	3.887218E-5	45.000004	15
GCAACTC	25	3.887218E-5	45.000004	25
TAGAACG	25	3.887218E-5	45.000004	1
TAGCGAG	25	3.887218E-5	45.000004	1
CCTACAA	25	3.887218E-5	45.000004	27
TCCTCGA	25	3.887218E-5	45.000004	39
GTCTATC	25	3.887218E-5	45.000004	40
CAATTCG	25	3.887218E-5	45.000004	1
ATATGCG	25	3.887218E-5	45.000004	1
TGCGGTC	25	3.887218E-5	45.000004	15
TAAAACG	25	3.887218E-5	45.000004	1
GCGTTAG	25	3.887218E-5	45.000004	1
ATTCACG	25	3.887218E-5	45.000004	28
TATCTAC	25	3.887218E-5	45.000004	18
GATATAG	25	3.887218E-5	45.000004	1
GTACTGG	50	2.1827873E-11	45.000004	2
TATTACG	50	2.1827873E-11	45.000004	1
AATTAGC	25	3.887218E-5	45.000004	15
TATAGCG	25	3.887218E-5	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content